Abstract：
:The mitochondrion depends upon the import of cytosolically synthesized preproteins for most of the proteins that comprise its structural elements and metabolic pathways. Here we have examined the influence of redox conditions on mitochondrial preprotein import and processing by mammalian mitochondria. Paraquat pretreatment of isolated mitochondria inhibited the subsequent import preornithine transcarbamylase (pOTC) in vitro. In intact cells oxidizing conditions led to decreased levels of mature OTC and accumulation of its preprotein. Implicating a mitochondrial import lesion, the fluorescence of pOTC-GFP (a protein in which the presequence of pOTC was fused to green fluorescent protein) transfected cells was decreased by paraquat treatment while cytosolic wild-type GFP remained largely unaffected. The accumulation of preproteins was enhanced by proteasome inhibitors. We observed that precursor proteins that failed to be imported, due to oxidizing conditions or an intrinsically slower import rate, are susceptible to degradation. Inhibition of the proteasome was also found to lead to higher levels of the translocase outer membrane protein 20 (Tom20) and to the perinuclear accumulation of mitochondria. These studies indicate that cellular redox conditions influence mitochondrial import, which, in turn, affects mitochondrial protein levels. A role for the proteasome in this process and in general mitochondrial function was also indicated.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Wright G,Terada K,Yano M,Sergeev I,Mori Mdoi
10.1006/excr.2000.5096keywords:
subject
Has Abstractpub_date
2001-02-01 00:00:00pages
107-17issue
1eissn
0014-4827issn
1090-2422pii
S0014-4827(00)95096-1journal_volume
263pub_type
杂志文章abstract：:Drosophila sHSPs (small heat shock proteins) are expressed in the absence of stress in specific regions of the central nervous system and in gonads of young adults flies. In these two organs, the sHSPs show a cell-specific and developmental stage-specific pattern of expression suggesting distinct regulation and functi...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1996.0052
更新日期：1996-02-25 00:00:00
abstract：:The characteristics underlying cytosolic free calcium oscillation were evaluated by superfused dual wave-length microspectrofluorometry of fura-2-loaded single acinar cells from rat pancreas. Application of a physiological concentration of cholecystokinin octapeptide (CCK) (20 pM) induced a small basal increase in cyt...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(90)90173-8
更新日期：1990-06-01 00:00:00
abstract：:A single cell of wild-type Dictyostelium discoideum forms a visible colony on a plastic dish in several days, but due to enhanced cell migration, amiB-null mutant cells scatter over a large area and do not form noticeable colonies. Here, with an aim to identify genes involved in cell migration, we isolated suppresser ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2007.12.002
更新日期：2008-03-10 00:00:00
abstract：:Lithium has been found to be a novel inhibitor of the terminal differentiation of Friend murine erythroleukemia cells. A general method for the quantitative analysis of differentiation inhibitors has been developed and used to compare the site of inhibition by lithium with that by vanadate. Lithium inhibits the commit...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(87)90012-7
更新日期：1987-02-01 00:00:00
abstract：:12-Lipoxygenase, an arachidonic acid metabolizing enzyme of the lipoxygenase pathway, has been implicated as a major factor in promoting prostate cancer progression and metastasis. The ability of 12-LOX to aggravate the disease was linked to its proangiogenic role. Recent studies clearly demonstrated that 12-LOX enhan...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2010.03.005
更新日期：2010-06-10 00:00:00
abstract：:Mesenchymal stem cells (MSCs) are frequently used as a therapeutic, but reliable imaging technique to longitudinally evaluate the engraftment of transplanted cells is inadequate. For magnetic resonance imaging (MRI), it is essential to understand the technical competence of in vitro stem cells labeling with iron oxide...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2016.12.025
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abstract：:Pirh2, a recently identified ubiquitin-protein ligase, has been reported to promote p53 degradation. Pirh2 physically interacts with p53 and promotes ubiquitination of p53 independently of MDM2. Like MDM2, Pirh2 is thought to participate in an autoregulatory feedback loop that controls p53 function. We have previously...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2006.07.005
更新日期：2006-10-15 00:00:00
abstract：:Rat myotubes in tissue culture form broad areas of close contact with the substrate. These areas often display two distinct, interdigitating sets of membrane domains. One, the "contact domain", is close to the substrate; the other, termed the "AChR domain", is further from the substrate and is rich in acetylcholine re...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(89)90261-9
更新日期：1989-06-01 00:00:00
abstract：:Over the last two decades molecular studies of inherited tumor syndromes that are associated with the development of kidney cancer have led to the identification of genes and biochemical pathways, which play key roles in the malignant transformation of renal epithelial cells. Some of these findings have broad biologic...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2012.02.026
更新日期：2012-05-15 00:00:00
abstract：:A temperature-sensitive mutant of rat fibroblast line 3Y1, 3Y1tsD123, is arrested in the G1 phase of the cell cycle at restrictive temperature. Using a human cDNA expression library, we succeeded in the molecular cloning of a cDNA (designated as D123 cDNA) that functionally complemented the temperature-sensitive mutat...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1996.0078
更新日期：1996-03-15 00:00:00
abstract：:Two HL60 clones (C12 and C13) totally insensitive to differentiation induction by dimethylsulfoxide (Me2SO) are described. They have been growing continuously in the presence of the inducer for more than 6 months. The morphological and cytochemical features of the two populations are quite similar to those of the orig...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(83)90275-6
更新日期：1983-08-01 00:00:00
abstract：:Cell surface beta-1,4-galactosyltransferase (GalTase) partially mediates a variety of cell interactions with laminin-containing matrices, including mesenchymal cell spreading and migration and neurite initiation, by binding to N-linked oligosaccharides within the E8 domain of laminin. Previous studies using indirect i...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(92)90350-h
更新日期：1992-07-01 00:00:00
abstract：:In the present study, we have tested the influence of inhibition of endocytosis by hypertonic medium on the regulation of cell surface insulin receptors. We show that active internalization of 125I-insulin is markedly inhibited by hypertonic media and that, in parallel, cell surface invaginations are significantly dim...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(89)90034-7
更新日期：1989-11-01 00:00:00
abstract：:Tumor hypoxia correlates to aggressive disease, and while this is explained by a variety of factors, one clue to understand this phenomena was the finding that hypoxia induces a de-differentiated, stem cell-like phenotype in neuroblastoma and breast tumor cells. The hypoxia inducible transcription factors (HIFs) are r...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2017.03.007
更新日期：2017-07-15 00:00:00
abstract：:Bovine papilloma virus-transformed hamster embryo fibroblasts (HEF-BPV) reacted to exposure to tricyclodecan-9-yl-xanthogenate (D609) with immediate reversion to the growth kinetics and the flat morphology of the untransformed parental cells. After six population doublings in the presence of D609, clones which display...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(85)90109-0
更新日期：1985-12-01 00:00:00
abstract：:JANEX-1/WHI-P131, a selective Janus kinase 3 (JAK3) inhibitor, has been shown to delay the onset of diabetes in the NOD mouse model. However, the molecular mechanism by which JANEX-1 protects pancreatic beta-cells is unknown. In the current study, we investigated the role of JANEX-1 on interleukin (IL)-1beta and inter...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2009.04.021
更新日期：2009-07-15 00:00:00
abstract：:A novel C3G isoform, designated p87C3G, lacking the most amino terminal region of the cognate protein has been found to be overexpressed in two CML cell lines, K562 and Boff 210, both expressing Bcr-Abl p210. p87C3G expression is also highly augmented in patients diagnosed with chronic myeloid leukemia (CML) Ph+, in c...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2005.12.007
更新日期：2006-04-01 00:00:00
abstract：:Heat shock factor 1 (Hsf1) is an ancient transcription factor that monitors protein homeostasis (proteostasis) and counteracts disturbances by triggering a transcriptional programme known as the heat shock response (HSR). The HSR is transiently activated and upregulates the expression of core proteostasis genes, inclu...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
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更新日期：2020-11-01 00:00:00
abstract：:The sodium-coupled bicarbonate cotransporter 1 (NBCe1) plays an essential role in the maintenance of acid-base homeostasis in the human body. However, little research has been done regarding the modification of NBCe1. Nedd4-2 is one of the most important ubiquitin E3 ligases in the kidney where it is responsible for m...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2020.111958
更新日期：2020-05-15 00:00:00
abstract：:Screening for microbial metabolites that induce transcriptional activation of the SV40 promoter resulted in the identification of two known compounds, FR901228 and trichostatin A (TSA). FR901228 is a potent antitumor drug that is currently under clinical investigation. TSA is a specific inhibitor of histone deacetylas...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1998.4027
更新日期：1998-05-25 00:00:00
abstract：:Both hypoxia and overexpression of the urokinase plasminogen activator receptor (uPAR) are associated with a poor clinical outcome in human cancers. Hypoxia has been shown to induce uPAR expression in breast cancer cells and to increase their invasion through Matrigel, a phenomenon which can be blocked using an anti-u...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1999.4804
更新日期：2000-03-15 00:00:00
abstract：:The goal of this study was to assess the in vitro differentiation capacity of human bone marrow-derived stem cells (hBMSCs) along retinal lineages. Mononuclear cells (MNC) were isolated from bone marrow (BM) and mobilized peripheral blood (mPB) using Ficoll-Paque density gradient centrifugation, and were sorted by mag...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2015.02.001
更新日期：2015-04-10 00:00:00
abstract：:Local regulation of the cortical cytoskeleton controls cell surface dynamics. GAP-43 and MARCKS are two abundant cytosolic protein kinase C substrates that are anchored to the cell membrane via acyl groups and interact with the cortical cytoskeleton. Each of them has been implicated in several forms of motility involv...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1997.3709
更新日期：1997-10-10 00:00:00
abstract：:CIN85 is a multidomain protein that associates with receptors carrying tyrosine kinase domains. Here we report that it is also a component of the signaling complex associated with tumor necrosis factor receptor 1 (TNFR1), which lacks a tyrosine kinase domain. This was established by showing that CIN85 was co-precipita...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2004.11.005
更新日期：2005-03-10 00:00:00
abstract：:The assumption that cells are temporally organized systems, i.e. showing relevant dynamics of their state variables such as gene expression or protein and metabolite concentration, while tacitly given for granted at the molecular level, is not explicitly taken into account when interpreting biological experimental dat...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2011.09.007
更新日期：2011-12-10 00:00:00
abstract：:Protein kinases are thought to play a key role in signal transduction and oncogenesis, but little is known about the intranuclear phosphorylation events associated with transformation. Here we report on cell cycle-dependent phosphorylation of cytoskeleton-associated 350 kD protein and the regular interchange in its lo...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(86)90169-2
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abstract：:Numerous factors that promote movement of macromolecules in and out of the nucleus have now been identified. These include both soluble cytoplasmic and nucleoplasmic proteins and proteins of the nuclear pore complex (NPC). Genetic analyses of the nuclear transport process in the model organism, the budding yeast Sacch...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1006/excr.1996.0362
更新日期：1996-12-15 00:00:00
abstract：:Barrier-to-autointegration factor (BAF) is an essential chromatin protein conserved in metazoans. BAF has roles in nuclear assembly, chromatin organization, gene expression, and gonad development and is exploited by retroviruses. BAF forms stable dimers that bind nonspecifically to dsDNA and specifically to LEM-domain...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2005.11.013
更新日期：2006-02-15 00:00:00
abstract：:Several lines of evidence indicate that telomere shortening during in vitro aging of human somatic cells plays a causal role in cellular senescence. A critical telomere length seems to be associated with the replicative block characterizing senescent cells. In this paper we analyzed the mean length of the terminal res...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1999.4398
更新日期：1999-04-10 00:00:00
abstract：:The retinoblastoma tumor suppressor protein (RB) is activated/dephosphorylated to mediate cell cycle inhibition in response to antimitogenic signals. To elucidate the mode of RB action at this critical transition, we utilized cell lines that can be induced to express a constitutively active allele of RB (PSM-RB). As e...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2002.5510
更新日期：2002-06-10 00:00:00
