Abstract：
:Tumor hypoxia correlates to aggressive disease, and while this is explained by a variety of factors, one clue to understand this phenomena was the finding that hypoxia induces a de-differentiated, stem cell-like phenotype in neuroblastoma and breast tumor cells. The hypoxia inducible transcription factors (HIFs) are regulated at the translational level by fluctuating oxygen concentrations, but emerging data reveal that both HIF-1α and HIF-2α expression can be induced by aberrantly activated growth factor signaling independently of oxygen levels. Furthermore, HIF-2α is regulated by hypoxia also at the transcriptional level in neuroblastoma and glioma cells. In cultured tumor cells, HIF-2α is stabilized at physiological oxygen concentrations followed by induced expression of classical hypoxia-driven genes, resulting in a pseudohypoxic phenotype. In addition, in neuroblastoma and glioma specimens, a small subset of HIF-2α positive, HIF-1α negative, tumor cells is found adjacent to blood vessels, i.e. in areas with presumably adequate oxygenation. These tumor niches are thus pseudohypoxic, and the HIF-2α expressing cells present immature features. We have postulated that this niche in neuroblastomas encompass the tumor stem cells. Oncogenes or tumor suppressor genes associated with pseudohypoxia are frequently mutated or deleted in the germline, implicating that the pseudohypoxic phenotype indeed is tumorigenic. In summary, the hypoxic and pseudohypoxic phenotypes of solid tumors are attractive therapeutic targets.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Mohlin S,Wigerup C,Jögi A,Påhlman Sdoi
10.1016/j.yexcr.2017.03.007subject
Has Abstractpub_date
2017-07-15 00:00:00pages
192-196issue
2eissn
0014-4827issn
1090-2422pii
S0014-4827(17)30113-1journal_volume
356pub_type
杂志文章,评审abstract：:We report the isolation of six cell lines (designated EB cell lines) from cultures of the hypoxanthine guanine phosphoribosyl transferase-deficient (HGPRT-) feeder-dependent embryonal carcinoma cell line PSA4TG12 which have undergone in vitro differentiation, and of clonal derivatives of these lines. Whereas some line...
journal_title：Experimental cell research
pub_type： 杂志文章
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journal_title：Experimental cell research
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journal_title：Experimental cell research
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journal_title：Experimental cell research
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pub_type： 杂志文章
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：1995-10-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：1982-11-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2004.01.021
更新日期：2004-06-10 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：1991-09-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：1989-11-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：1986-05-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2011.08.020
更新日期：2012-01-01 00:00:00
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pub_type： 杂志文章
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更新日期：2017-03-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章,评审
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pub_type： 杂志文章
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更新日期：1999-08-25 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：1991-03-01 00:00:00
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journal_title：Experimental cell research
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更新日期：1984-03-01 00:00:00
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pub_type： 杂志文章
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更新日期：2019-01-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2019.03.029
更新日期：2019-06-01 00:00:00
abstract：:Using a device named the cell force monitor, the contractile force developed by fibroblasts has been studied by measuring the macroscopic contraction of porous collagen-glycosaminoglycan (GAG) matrices over the first 24 h following cell attachment. In this paper, the effect of a variation in the stiffness that resists...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2001.5408
更新日期：2002-01-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：1991-12-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2004.05.025
更新日期：2004-09-10 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2004.06.024
更新日期：2004-10-01 00:00:00
abstract：:The low density lipoprotein receptor-related protein (LRP) binds multiple, distinct ligands and participates in constitutive endocytosis and signal transduction. Using an in vitro reconstitution system and a new biochemical complementation assay, we have explored the limiting cytosolic requirements for endocytosis of ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2005.12.022
更新日期：2006-05-01 00:00:00
