12-Lipoxygenase and the regulation of hypoxia-inducible factor in prostate cancer cells.


:12-Lipoxygenase, an arachidonic acid metabolizing enzyme of the lipoxygenase pathway, has been implicated as a major factor in promoting prostate cancer progression and metastasis. The ability of 12-LOX to aggravate the disease was linked to its proangiogenic role. Recent studies clearly demonstrated that 12-LOX enhances the expression and secretion of the angiogenic factor, vascular endothelial growth factor (VEGF) thus providing a direct link between this enzyme and its angiogenic properties. In the present study we have investigated the relationship between 12-LOX and hypoxia inducible factor-1alpha (HIF-1alpha), a transcription factor involved in the regulation of VEGF expression under hypoxic conditions in solid tumors. Our findings have revealed that HIF-1 is one of the target transcription factors regulated by 12-LOX and 12(S)-HETE, in hypoxic tumor cells of the prostate. Regulation of HIF-1alpha by 12-LOX adds to the complexity of pathways mediated by this enzyme in promoting prostate cancer angiogenesis and metastasis. We have evidence that 12-LOX increases the protein level, mRNA, and functional activity of HIF-1alpha under hypoxic conditions, one of the mechanisms by which it upregulates VEGF secretion and activity.


Exp Cell Res


Krishnamoorthy S,Jin R,Cai Y,Maddipati KR,Nie D,Pagès G,Tucker SC,Honn KV




Has Abstract


2010-06-10 00:00:00














  • Antibodies against Tetrahymena 14-nm filament-forming protein recognize the replication band in Euplotes.

    abstract::Tetrahymena 14-nm filament-forming protein (49K protein) is a structural protein which is involved in activity of the pronuclei during conjugation (O. Numata, T. Sugai, and Y. Watanabe (1985) Nature (London) 314, 192-194). Using monoclonal and polyclonal antibodies, we here demonstrate the presence of a cross-reactive...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Numata O,Tomiyoshi T,Kurasawa Y,Fu ZX,Takahashi M,Kosaka T,Chiba J,Watanabe Y

    更新日期:1991-03-01 00:00:00

  • High glucose increases glomerular filtration barrier permeability by activating protein kinase G type Iα subunits in a Nox4-dependent manner.

    abstract::Hyperglycemia is a primary factor that disturbs podocyte function in the glomerular filtration process; this disturbance leads to the development of diabetic nephropathy, and ultimately, renal failure. Podocyte function may also be altered by biological agents that modify protein kinase activity, including the cGMP-ac...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Piwkowska A,Rogacka D,Audzeyenka I,Angielski S,Jankowski M

    更新日期:2014-01-01 00:00:00

  • A-type nuclear lamins act as transcriptional repressors when targeted to promoters.

    abstract::Regions of heterochromatin are often found at the periphery of the mammalian nucleus, juxtaposed to the nuclear lamina. Genes in these regions are likely maintained in a transcriptionally silent state, although other locations at the nuclear periphery associated with nuclear pores are sites of active transcription. As...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Lee DC,Welton KL,Smith ED,Kennedy BK

    更新日期:2009-04-01 00:00:00

  • A C-terminal PDZ binding domain modulates the function and localization of Kv1.3 channels.

    abstract::The voltage-gated potassium channel, Kv1.3, plays an important role in regulating membrane excitability in diverse cell types ranging from T-lymphocytes to neurons. In the present study, we test the hypothesis that the C-terminal PDZ binding domain modulates the function and localization of Kv1.3. We created a mutant ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Doczi MA,Damon DH,Morielli AD

    更新日期:2011-10-01 00:00:00

  • Phosphorylation of filamin A by Cdk1 regulates filamin A localization and daughter cell separation.

    abstract::In cell culture, many adherent mammalian cells undergo substantial actin cytoskeleton rearrangement prior to mitosis as they detach from the extracellular matrix and become spherical. At the end of mitosis, the actin cytoskeleton is required for cytokinesis and the reassembly of interphase structures as cells spread a...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Szeto SGY,Williams EC,Rudner AD,Lee JM

    更新日期:2015-01-15 00:00:00

  • Differentiation-linked expression of prothymosin alpha gene in human myeloid leukemic cells.

    abstract::Prothymosin alpha (ProT alpha) is a nuclear protein related to cell proliferation. Its gene is highly activated during postnatal development at stages containing many proliferating but also differentiating cells. In this report, a study on ProT alpha gene expression during differentiation of human myeloid leukemic (HL...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Dosil M,Alvarez-Fernández L,Gómez-Márquez J

    更新日期:1993-01-01 00:00:00

  • MZF-1 and DbpA interact with DNase I hypersensitive sites that correlate with expression of the human MUC1 mucin gene.

    abstract::The MUC1 mucin is a large membrane-tethered glycoprotein that shows differential expression in many adenocarcinomas, where it contributes to their invasive and metastatic properties. We previously identified DNase I hypersensitive sites at -750 and -250 bp in the human MUC1 gene promoter and showed concordance between...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Shiraga T,Winpenny JP,Carter EJ,McCarthy VA,Hollingsworth MA,Harris A

    更新日期:2005-08-01 00:00:00

  • Cell cycle-dependent DNA damage signaling induced by ICRF-193 involves ATM, ATR, CHK2, and BRCA1.

    abstract::Topoisomerase II is essential for cell proliferation and survival and has been a target of various anticancer drugs. ICRF-193 has long been used as a catalytic inhibitor to study the function of topoisomerase II. Here, we show that ICRF-193 treatment induces DNA damage signaling. Treatment with ICRF-193 induced G2 arr...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Park I,Avraham HK

    更新日期:2006-07-01 00:00:00

  • Expression of lamin A mutated in the carboxyl-terminal tail generates an aberrant nuclear phenotype similar to that observed in cells from patients with Dunnigan-type partial lipodystrophy and Emery-Dreifuss muscular dystrophy.

    abstract::Autosomal dominantly inherited missense mutations in lamins A and C cause familial partial lipodystrophy of the Dunnigan-type (FPLD), and myopathies including Emery-Dreifuss muscular dystrophy (EDMD). While mutations responsible for FPLD are restricted to the carboxyl-terminal tails, those responsible for EDMD are spr...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Favreau C,Dubosclard E,Ostlund C,Vigouroux C,Capeau J,Wehnert M,Higuet D,Worman HJ,Courvalin JC,Buendia B

    更新日期:2003-01-01 00:00:00

  • Disintegration of adhesion plaques in chicken embryo fibroblasts upon Rous sarcoma virus-induced transformation: different dissociation rates for talin and vinculin.

    abstract::The localization of talin and vinculin in chicken embryo fibroblasts (CEF) during transformation was studied by immunoelectron microscopy. CEF cells were infected with a temperature-sensitive mutant of Rous sarcoma virus. After 16 h at 42 degrees C, transformation was induced by incubation at 37 degrees C for differen...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Brands R,de Boer A,Feltkamp CA,Roos E

    更新日期:1990-01-01 00:00:00

  • Basal lamina formation by epithelial cell lines correlates with laminin A chain synthesis and secretion.

    abstract::Laminin is a major component of the basal lamina upon which all epithelial cells reside in vivo. The synthesis of basal lamina components and their subsequent assembly into a morphologically distinct basal lamina is a differentiated function of epithelial cells in vivo. Ultrastructural studies in our laboratory show t...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Ecay TW,Valentich JD

    更新日期:1992-11-01 00:00:00

  • Downregulation of the growth hormone-induced Janus kinase 2/signal transducer and activator of transcription 5 signaling pathway requires an intact actin cytoskeleton.

    abstract::Transient activation of the signal transducers and activators of transcription (STAT) proteins in response to growth hormone (GH) and other type II cytokines plays a pivotal role on specific gene transcription. The negative regulation of STATs seems to be exerted at the GH receptor (GHR)/Janus Kinase (JAK) complex and...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Rico-Bautista E,Negrín-Martínez C,Novoa-Mogollón J,Fernández-Perez L,Flores-Morales A

    更新日期:2004-03-10 00:00:00

  • Intracellular protein delivery activity of peptides derived from insulin-like growth factor binding proteins 3 and 5.

    abstract::Insulin-like growth factor binding proteins (IGFBPs) have various IGF-independent cellular activities, including receptor-independent cellular uptake followed by transcriptional regulation, although mechanisms of cellular entry remain unclear. Herein, we focused on their receptor-independent cellular entry mechanism i...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Goda N,Tenno T,Inomata K,Shirakawa M,Tanaka T,Hiroaki H

    更新日期:2008-08-01 00:00:00

  • Modification of integrin-mediated cell attachment to substrata by serine proteinases in the presence and absence of divalent cations.

    abstract::The sensitivity to serine proteinases of cellular proteins involved in cell-matrix adhesion was investigated using C32 melanoma cells. Cells dissociated from monolayers by the metal chelator ethylenediaminetetraacetic acid were incubated with proteolytic enzymes, and then attachment was quantified by standard cell adh...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Fujii K,Lazarus GS,Schechter NM

    更新日期:1993-09-01 00:00:00

  • CHO cell aggregation induced by fibronectin-coated beads. Differences between wild-type and adhesion-variant cells.

    abstract::ADvF11 (F11), a Chinese Hamster Ovary (CHO) cell variant, is defective in its ability to adhere to fibronectin (Fn)-coated substrata but will adhere to substrata coated with poly-L-lysine, conA or extracellular matrix (ECM) [1]. We have observed that both F11 and CHO wild-type (WT) cells were able to bind 3H-Fn beads ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Cheung E,Juliano RL

    更新日期:1984-05-01 00:00:00

  • Expression of different mutant p53 transgenes in neuroblastoma cells leads to different cellular responses to genotoxic agents.

    abstract::The involvement of p53 as a determinant of chemosensitivity or radiosensitivity is not well understood and is complicated by numerous contradictory reports. Here we have addressed this issue using a series of isogenic clones derived from two neuroblastoma cell lines that express wild-type p53 genes, Nub7 and IMR32. Tw...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Gangopadhyay S,Jalali F,Reda D,Peacock J,Bristow RG,Benchimol S

    更新日期:2002-04-15 00:00:00

  • Significant variations in differentiation properties between independent mouse ES cell lines cultured under defined conditions.

    abstract::Mouse embryonic stem (ES) cells are isolated from the inner cell mass (ICM)/epiblast of preimplantation embryos and are widely used in cell differentiation studies. We have previously observed differences in transcript and antigen expression following differentiation of ES cells lines in vitro. We have investigated th...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Ward CM,Barrow KM,Stern PL

    更新日期:2004-02-15 00:00:00

  • Turbidimetric studies on microtubule sliding using the stopped-flow-light-scattering method.

    abstract::The turbidity of axonemes during active sliding of microtubules was analysed using the stopped-flow-light-scattering method with high time resolution. Flagella of sea-urchin spermatozoa were demembranated and used after a brief treatment with trypsin. The turbidity of the suspension of flagellar axonemes during ATP-in...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Kamimura S,Nakanishi M,Yano M,Shimizu H

    更新日期:1986-03-01 00:00:00

  • Glucocorticoid hormones may partially substitute for adenovirus E1A in cooperation with ras.

    abstract::The effects of hormonal promotion of T24-ras oncogene-transfected rat embryo fibroblasts (REF) were compared to cotransformation of these cells with adenovirus E1A and ras. Cotransfection of E1A + ras resulted in the appearance of morphologically transformed cells which were very efficiently established into cell line...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Marshall H,Martens I,Svensson C,Akusjärvi G,Linder S

    更新日期:1991-05-01 00:00:00

  • Trefoil peptides promote restitution of wounded corneal epithelial cells.

    abstract::The ocular surface shares many characteristics with mucosal surfaces. In both, healing is regulated by peptide growth factors, cytokines, and extracellular matrix proteins. However, these factors are not sufficient to ensure most rapid healing. Trefoil peptides are abundantly expressed epithelial cell products which e...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Göke MN,Cook JR,Kunert KS,Fini ME,Gipson IK,Podolsky DK

    更新日期:2001-04-01 00:00:00

  • Actin organization during the cell cycle in meristematic plant cells. Actin is present in the cytokinetic phragmoplast.

    abstract::The distribution and organisation of F-actin during the cell cycle of meristematic root-tip cells of Allium was investigated using a rhodamine-labelled phalloidin to stain F-actin in isolated cell preparations. Such preparations could, in addition, be stained for tubulin by immunofluorescence, enabling a comparison be...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Clayton L,Lloyd CW

    更新日期:1985-01-01 00:00:00

  • Mitochondrial DNA is synthesized during meiosis and spermiogenesis in the mouse.

    abstract::Following intratesticular injection of [3H]thymidine, spermatozoa were isolated from the caput and cauda epididymis of CD-1 mice. Quantitation of the amount of radiolabelled DNA in whole spermatozoa, sperm heads (nuclei) and sperm midpiece-tails (mitochondria) at time intervals 3-36 days postinjection demonstrated mit...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Hecht NB,Liem H

    更新日期:1984-09-01 00:00:00

  • Osteopontin is associated with nuclear factor kappaB gene expression during tail-suspension-induced bone loss.

    abstract::Osteoporosis due to unloading-induced bone loss is a critical issue in the modern aging society. Although the mechanisms underlying this phenomenon are largely unknown, osteopontin (OPN) is one of the critical mediators required for unloading-induced bone loss [M. Ishijima, S.R. Rittling, T. Yamashita, K. Tsuji, H. Ku...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Ishijima M,Ezura Y,Tsuji K,Rittling SR,Kurosawa H,Denhardt DT,Emi M,Nifuji A,Noda M

    更新日期:2006-10-01 00:00:00

  • Multiple proteases are involved in thymocyte apoptosis.

    abstract::To investigate the involvement of proteases in apoptosis, rat thymocytes were treated with the glucocorticoid hormone methylprednisolone or the topoisomerase II inhibitor etoposide in the presence of selective substrate inhibitors of either interleukin-1 beta-converting enzyme (ICE), (Z-Val-Ala-Asp-chloromethylketone,...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Zhivotovsky B,Gahm A,Ankarcrona M,Nicotera P,Orrenius S

    更新日期:1995-12-01 00:00:00

  • An integral membrane protein from the nuclear pore complex is also present in the annulate lamellae: implications for annulate lamella formation.

    abstract::Annulate lamellae (AL) are cytoplasmic arrays of stacked membrane cisternae containing densely packed pore complexes which are similar in structure to the nuclear pore complexes (NPCs) and thus referred to as annulate lamella pore complexes (ALPCs). We have recently shown that the integral nuclear pore membrane protei...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Imreh G,Hallberg E

    更新日期:2000-08-25 00:00:00

  • Nuclear translocation of a leukocyte elastase Inhibitor/Elastase complex during staurosporine-induced apoptosis: role in the generation of nuclear L-DNase II activity.

    abstract::Using L1210 murine leukemia cells, we have previously shown that in response to treatment with drugs having different targets, apoptotic cell death occurs through at least two different signaling pathways. Here, we present evidence that nuclear extracts from staurosporine-treated cells elicit DNase II activity that is...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Belmokhtar CA,Torriglia A,Counis MF,Courtois Y,Jacquemin-Sablon A,Ségal-Bendirdjian E

    更新日期:2000-01-10 00:00:00

  • Basic FGF and TGF-beta differentially modulate integrin expression of human microvascular endothelial cells.

    abstract::Basic fibroblast growth factor (bFGF) and transforming growth factor-beta (TGF-beta) are known to alter the migratory and proliferative capacity of endothelial cells in vitro and to stimulate angiogenesis in vivo. One mechanism by which these cytokines induce their effects may be through the regulation of integrin adh...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Enenstein J,Waleh NS,Kramer RH

    更新日期:1992-12-01 00:00:00

  • Molecular mechanisms of mechanotransduction in integrin-mediated cell-matrix adhesion.

    abstract::Cell-matrix adhesion complexes are multi-protein structures linking the extracellular matrix (ECM) to the cytoskeleton. They are essential to both cell motility and function by bidirectionally sensing and transmitting mechanical and biochemical stimulations. Several types of cell-matrix adhesions have been identified ...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Li Z,Lee H,Zhu C

    更新日期:2016-11-15 00:00:00

  • The zeta isozyme of protein kinase C binds to tubulin through the pseudosubstrate domain.

    abstract::It has been suggested that the protein kinase C zeta (zeta PKC) isoform is involved in mitogenic signaling in Xenopus oocytes and mammalian cells. Thus, the characterization of potential regulatory molecules that bind to zeta PKC is of great interest. We report here the identification by affinity chromatography of tub...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: García-Rocha M,Avila J,Lozano J

    更新日期:1997-01-10 00:00:00

  • Serum and serotonin induce retraction of calf aortic smooth muscle (CASM) cells in vitro: inhibition by ketanserin, a 5-HT2 receptor antagonist.

    abstract::Calf aortic smooth muscle (CASM) cells cultured in vitro at high cell density (4 x 10(4) cells/cm2) on bacteriological petri dishes in the presence of serum pile up in clusters and create open spaces in the monolayer. This phenomenon is clearly visible 6 days after plating and is markedly enhanced by the addition of f...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: De Wever B,Rooman RP,De Brabander M

    更新日期:1990-01-01 00:00:00