Abstract:
:We have studied the internalization of targeted fusogenic liposome content to leukemic T cells (CEM) in vitro. We describe a method for the covalent coupling of T101 antibody to the surface of liposomes and the incorporation of fusogenic viral protein into the liposome membrane. Hygromycin B, an impermeant inhibitor of protein synthesis, was encapsulated in the targeted fusogenic liposomes and delivered directly to the cytoplasm of leukemic T cells by fusion between the two membranes. The cytotoxic effect was measured by [3H]thymidine incorporation. We show that CEM are rapidly and specifically killed by the drug encapsulated in the targeted fusogenic liposomes. This effect is due to the binding of the liposome by means of the antibody and then to the fusion of the liposome with the targeted cell membrane, mediated by F protein.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Sechoy O,Vidal M,Philippot JR,Bienvenue Adoi
10.1016/0014-4827(89)90042-6subject
Has Abstractpub_date
1989-11-01 00:00:00pages
122-31issue
1eissn
0014-4827issn
1090-2422pii
0014-4827(89)90042-6journal_volume
185pub_type
杂志文章abstract::Terminally differentiated skeletal muscle myotubes are arrested in G0 phase of the cell cycle and are unable to be released from this arrest by stimulation with mitogens including serum and growth factors. To inspect a possibility of reversing the quiescence at the G0 phase, we have exploited the mouse skeletal muscle...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1994.1258
更新日期:1994-09-01 00:00:00
abstract::A new beta 1-containing integrin was isolated from rat liver by affinity chromatography on Sepharose conjugated with the peptide GRGDSPC. The interaction was weakened but not abolished when the arginine and/or aspartic acid in the peptide were replaced with lysine and glutamic acid, respectively. In contrast, the cyst...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1994.1189
更新日期:1994-07-01 00:00:00
abstract::Alternative splicing is an important mechanism that can disrupt cell cycle control resulting in tumorigenesis. Although many alterations of Transforming Growth Factor Beta (TGFβ) signaling are reported in cancers, the role of splice aberrations in destabilizing this signaling is the least understood mechanism. In this...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2014.05.004
更新日期:2014-09-10 00:00:00
abstract::Poly(ADP-ribosyl)ation (PARylation) is a post-translational modification of proteins catalysed by Poly(ADP-ribose) polymerases (PARP). A wealth of recent advances in the biochemical and functional characterization of the DNA-dependent PARP family members have highlighted their key contribution in the DNA damage respon...
journal_title:Experimental cell research
pub_type: 杂志文章,评审
doi:10.1016/j.yexcr.2014.07.003
更新日期:2014-11-15 00:00:00
abstract::The importance of mononuclear leukocyte (MO) adhesion to dysfunctional endothelium and migration to the subendothelial space in the early phases of atherogenesis is well established. Few studies have addressed the relevance of polymorphonuclear leukocytes (PMNs) in the context of evolving lesions, and nothing is known...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2001.5459
更新日期:2002-04-01 00:00:00
abstract::PDGF acts as an autocrine and paracrine factor in certain tumors through upregulation of the PDGF beta-receptor expression. In order to elucidate the control mechanism for the receptor expression, we have isolated an enhancer from two P1 clones that together contain a 102 kb NotI region covering the entire human PDGFR...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2006.03.005
更新日期:2006-07-01 00:00:00
abstract::Translation is mediated partly by regulation of free eukaryotic initiation factor 4E (eIF4E) levels through PI3K-Akt-mTOR signaling. Cancer cells treated with the plant-derived perillyl alcohol (POH) or the mechanistic target of rapamycin (mTOR) inhibitor rapamycin dephosphorylate eIF4E-binding protein (4E-BP1) and at...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2013.05.029
更新日期:2013-08-01 00:00:00
abstract::The protein encoded by C-terminal alternatively spliced p53 mRNA (p53as) has been shown previously to occur naturally in mouse cells and to bind sequence-specifically to DNA more efficiently than p53 (p53r, regular form). In the current study, p53as and p53r proteins ectopically expressed in p53-deficient cells each t...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2002.5596
更新日期:2002-10-01 00:00:00
abstract::Chronic lymphocytic leukemia (CLL) results from the uncontrolled proliferation and accumulation of B-1 cells, many of which demonstrate self-reactivity. The response of B-1 cells to mitogen after undergoing malignant transformation is still unclear. Using our established malignant B-1 cell lines derived from the NZB m...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2000.5122
更新日期:2001-04-01 00:00:00
abstract::Previous studies have demonstrated that treatment of fibroblasts from confluent, density-inhibited cultures with 50 mM KCl solution led to the onset of DNA replication. In the present study we show that such treatment can induce aphidicolin-sensitive DNA replication in differentiated nondividing cells: dorsal root gan...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1993.1296
更新日期:1993-11-01 00:00:00
abstract::Bone morphogenetic proteins (BMPs) are multifunctional signaling molecules that have gained increasing interest in cancer research. To obtain a systematic view on BMP signaling in pancreatic cancer we first determined the mRNA expression levels of seven BMP ligands (BMP2-BMP8) and six BMP specific receptors in pancrea...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2011.06.001
更新日期:2011-09-10 00:00:00
abstract::Skin fibroblasts from patients with inherited adenomatosis of the large bowel (ACR-SF) possess alterations in actin microfilament (MF) organization which serve to distinguish "predisposed" cells from fibroblasts derived from normal individuals (NSF). MF bundle frequency and diameter were considerably reduced in ACR-SF...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(91)90389-c
更新日期:1991-08-01 00:00:00
abstract::The ccl mutation in Paramecium tetraurelia reversibly and rapidly blocks cell cycle progression and DNA synthesis at the restrictive temperature. Progression through the cell cycle is blocked during both the G1 and S portions of the cell cycle, while at the restrictive temperature there is neither residual cell cycle ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(84)90220-9
更新日期:1984-12-01 00:00:00
abstract::Bone morphogenetic protein (BMP), a member of the TGF-beta superfamily, is involved in development, morphogenesis, cell proliferation and apoptosis. Dysregulation of BMP signaling has been suggested in tumorigenesis. In an analysis of human colon normal mucosa and tumors at different stages by immunohistochemistry, we...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2006.12.020
更新日期:2007-03-10 00:00:00
abstract::Oligomerization of Fas receptor by its ligand, FasL, activates a signaling cascade that leads to apoptosis of Fas bearing cells. Interestingly, many epithelia coexpress Fas and FasL, yet FasL does not trigger Fas present on the same or neighboring cells to induce spontaneous apoptosis. Here, we show that Fas and FasL ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/s0014-4827(02)00056-3
更新日期:2003-04-01 00:00:00
abstract::The results of Phase I/II clinical trials indicate that ricin A-chain-containing immunotoxins cause vascular leak syndrome, characterized by hypoalbuminemia with resultant weight gain and edema. Vascular leak syndrome may be a dose-limiting factor during treatment with ricin A-chain-containing immunotoxins. In this re...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1993.1142
更新日期:1993-06-01 00:00:00
abstract::Psoriasis is a chronic inflammatory skin disease severely affecting patients' physical and psychological well-being. Aberrant keratin expression in psoriasis plays a crucial role in keratinocyte dysfunction and disease development. Little is yet known about the mechanism of this keratin dysregulation. VEGF (Vascular e...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2017.09.021
更新日期:2017-11-15 00:00:00
abstract::Polo-like kinases (Plks) are essential for progression through mitosis. The activity of these kinases peak during M phase and this activation has been attributed to phosphorylation. Kinases capable of activating Plks in vitro have been previously identified both in mammalian cells and in Xenopus laevis oocytes (SLK an...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2005.10.033
更新日期:2006-02-01 00:00:00
abstract::The effect of hydrocortisone (HC) on PDGF beta-receptor expression was studied in the human malignant mesothelioma cell line Mero-14. HC was found to induce a time- and dose-dependent increase of PDGF beta-receptor mRNA. Nuclear run off analysis revealed that HC induced increased transcription of the PDGF beta-recepto...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/s0014-4827(05)80074-6
更新日期:1992-05-01 00:00:00
abstract::The fruit fly Drosophila melanogaster has recently emerged as an excellent system to investigate the genetics of cardiovascular development and disease. Drosophila provides an inexpensive and genetically-tractable in vivo system with a large number of conserved features. In addition, the Drosophila embryo is transpare...
journal_title:Experimental cell research
pub_type: 杂志文章,评审
doi:10.1016/j.yexcr.2017.03.057
更新日期:2017-09-01 00:00:00
abstract::In primary cultures of ovine thyroid cells, TSH induced the expression of several differentiated functions including the formation of follicles, and synthesis and storage of iodinated thyroglobulin in the follicular lumen. In the present report, these follicles were shown by transmission (TEM) and scanning electron mi...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(86)90031-5
更新日期:1986-06-01 00:00:00
abstract::Multidrug-resistant (MDR) cells demonstrate the increased activity of the membrane transport system performing efflux of diverse lipophylic drugs and fluorescent dyes from the cells. In order to detect MDR cells we have developed a simple test consisting of three steps: staining of the cells with fluorescent dye rhoda...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(89)90318-2
更新日期:1989-12-01 00:00:00
abstract::Adriamycin, an anticancer agent acting on topoisomerase II, promotes the arrest of cell division and neurite extension in a "neurite-minus" murine neuroblastoma cell line, N1A-103. This morphological differentiation is accompanied by a blockade in the S phase of the cell cycle, modification of the amount of peripherin...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(92)90041-6
更新日期:1992-11-01 00:00:00
abstract::Hypoxia can activate the endothelium toward a pro-inflammatory phenotype and enhance leukocyte adhesion. This process is involved in pathological conditions such as vascular remodeling or ischemia-reperfusion injury. This study was aimed to obtain a global picture of the response of the endothelial cells to hypoxia wi...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2008.11.020
更新日期:2009-03-10 00:00:00
abstract::Nuclear and territorial positioning of p- and q-telomeres and centromeres of chromosomes 3, 8, 9, 13, and 19 were studied by repeated fluorescence in situ hybridization, high-resolution cytometry, and three-dimensional image analysis in human blood lymphocytes before and after stimulation. Telomeres were found on the ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/s0014-4827(03)00208-8
更新日期:2003-09-10 00:00:00
abstract::The tumor-associated immune response is governed by the signalling events of various regulatory molecules, one of which is the cytotoxic T lymphocyte-associated antigen 4 (CTLA-4). In conventional T cells, CTLA-4 could outcompete CD28 in binding to CD80/86 but does not produce a co-stimulatory signal, resulting in T c...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2017.09.030
更新日期:2017-11-15 00:00:00
abstract::Mip130/LIN-9 is part of a large complex that includes homologs of the Drosophila dREAM (drosophila RB-like, E2F, and Myb) and C. elegans DRM complexes. This complex also includes proteins such as Mip40/LIN-37, Mip120/LIN-54, and LIN-52. In mammalian cells, Mip130/LIN-9 specifically associates with the p107/p130-E2F4 r...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2009.07.014
更新日期:2009-10-15 00:00:00
abstract::We examined the transcriptional activity profile of the gene for atrial natriuretic factor (ANF) in mouse embryonal carcinoma P19 cells which had been induced for in vitro cardiac myogenesis. Differentiation was assessed visually, by the degree of spontaneous beating activity, and by the appearance of striated muscle ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1993.1210
更新日期:1993-08-01 00:00:00
abstract::Cisplatin resistance in cancer cells is due to a pleiotropic phenotype transition that allows cells to resist cell death. miRNAs have been shown to be reliable markers of phenotype, critical in cell differentiation, and dysregulated in cancer and other pathologies. Here we investigate the influence of miRNA on cisplat...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2012.10.012
更新日期:2013-02-15 00:00:00
abstract::In preliminary experiments it was established that the hypertrophic and hyperplastic responses of neonatal cardiac myocytes in culture were associated with enhanced expression of IGF-1 and IGF-1 receptors in these cells. Therefore, to determine the role of IGF-1 receptors on myocyte growth, cells were exposed to antis...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1994.1343
更新日期:1994-12-01 00:00:00