The role of B-cell-specific activator protein in the response of malignant B-1 cells to LPS.

Abstract:

:Chronic lymphocytic leukemia (CLL) results from the uncontrolled proliferation and accumulation of B-1 cells, many of which demonstrate self-reactivity. The response of B-1 cells to mitogen after undergoing malignant transformation is still unclear. Using our established malignant B-1 cell lines derived from the NZB murine model of human CLL, we investigated the response of malignant B-1 cells to the mitogen LPS. Interestingly, these malignant B-1 cells proliferated initially, but the proliferation rate decreased after a 48-h transition. Prolonged LPS treatment induced apoptosis and pathological differentiation. We studied possible underlying molecular mechanisms and found that the level of the DNA binding protein BSAP (B-cell-specific activator protein) was upregulated by LPS at the initial activation stage, followed by an increase in the apoptotic factor caspase-3 (CPP32) at 48 h and a subsequent decrease of BSAP at 72 h. The pathological differentiation induced by LPS was partially prevented by treatment with antisense BSAP. This study indicates that malignant B-1 cells could be driven to apoptosis and pathological differentiation when activated by the mitogen LPS, and BSAP may be an important factor in regulating these responses.

journal_name

Exp Cell Res

authors

Zhang M,Chong SY,Raveche ES

doi

10.1006/excr.2000.5122

keywords:

subject

Has Abstract

pub_date

2001-04-01 00:00:00

pages

233-43

issue

2

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(00)95122-X

journal_volume

264

pub_type

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