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Repression of transcription and interference with DNA binding of TATA-binding protein by C-terminal alternatively spliced p53.

Abstract：

:The protein encoded by C-terminal alternatively spliced p53 mRNA (p53as) has been shown previously to occur naturally in mouse cells and to bind sequence-specifically to DNA more efficiently than p53 (p53r, regular form). In the current study, p53as and p53r proteins ectopically expressed in p53-deficient cells each transactivated reporter plasmids containing p53 binding sites. However, p53as consistently was more efficient in transcriptional repression of promoters lacking p53 binding sites and in concentration-dependent repression of the p21(WAF1/Cip-l/Sdi) promoter sequence. The p53as protein, like p53r, associated with TATA-binding protein (TBP), indicating that this interaction does not require the last 26 amino acids of p53. Consistent with its stronger repression effects, p53as interfered with TBP binding to a TATA-containing DNA sequence more efficiently than p53r protein. Taken together, these in vitro and in vivo results demonstrate a novel role in transcriptional repression for a naturally occurring C-terminal variant form of mouse p53 protein associated with differences in DNA binding properties and interference with transcription factor binding.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Huang H,Kaku S,Knights C,Park B,Clifford J,Kulesz-Martin M

doi

10.1006/excr.2002.5596

keywords:

subject

Has Abstract

pub_date

2002-10-01 00:00:00

pages

248-59

issue

2

eissn

0014-4827

issn

1090-2422

pii

S0014482702955965

journal_volume

279

pub_type

杂志文章

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