Abstract：
:Embryonic stem (ES) cells have been tested for potential cell transplantation therapy for CNS disorders. Understanding their differentiation mechanism and identifying factors involved in driving excitatory and inhibitory neuron lineages should enhance the efficacy and efficiency of the cell transplantation therapy. We tested the hypothesis that selective expression of Src family tyrosine kinases is required for phenotype-specific differentiation and functional maturation of ES cell derived neurons. Cultured mouse pluripotent ES cells were treated with retinoic acid (RA) to induce neural differentiation. After RA induction, neurons derived from ES cells showed significant neurite growth, increased expression of Src, Fyn and Lck and an extension of Src kinase expression from cell body to neurite processes. ES cell derived neuron-like cells expressed neurofilament, synaptophysin, glutamate receptors, NMDA and kainate currents, became vulnerable to excitotoxicity and formed functional excitatory synapses. These developmental events were blocked or attenuated when cells were grown in the presence of Src family kinase inhibitor PP2. However, there was no change in the expression of GABAergic-specific protein GAD67 during PP2 treatment. Our data suggest that Src tyrosine kinases are involved in the terminal differentiation of excitatory neuronal phenotype during ES cell neural differentiation after RA induction.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Theus MH,Wei L,Francis K,Yu SPdoi
10.1016/j.yexcr.2006.06.022subject
Has Abstractpub_date
2006-10-01 00:00:00pages
3096-107issue
16eissn
0014-4827issn
1090-2422pii
S0014-4827(06)00222-9journal_volume
312pub_type
杂志文章abstract：:Regulation of TSH receptor (TSHr) mRNA accumulation has been investigated in canine thyrocytes in primary culture by in situ hybridization experiments; the effects of the mitogenic thyrotropin (TSH), epidermal growth factor (EGF), and phorbol ester TPA (12-O-tetradecanoylphorbol-13-acetate) have been compared. Apart f...
journal_title：Experimental cell research
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2002.5512
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abstract：:After epithelial disruption by tissue injury, keratinocytes migrate from the wound edge into a provisional matrix. This process is stimulated by growth factors that signal through epidermal growth factor (EGF) receptor, including EGF, heparin-binding EGF-like growth factor (HB-EGF) and transforming growth factor-alpha...
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journal_title：Experimental cell research
pub_type： 杂志文章
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：1998-07-10 00:00:00
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pub_type： 杂志文章
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更新日期：1999-02-25 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/s0014-4827(05)80080-1
更新日期：1992-05-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1995.1149
更新日期：1995-05-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(83)90148-9
更新日期：1983-07-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(88)90357-6
更新日期：1988-11-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2006.10.004
更新日期：2007-01-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2007.04.010
更新日期：2007-06-10 00:00:00
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journal_title：Experimental cell research
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journal_title：Experimental cell research
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journal_title：Experimental cell research
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更新日期：2017-03-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：2015-01-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：2019-01-01 00:00:00
