当前位置： SCI文献检索 > EXPERIMENTAL CELL RESEARCH期刊下所有文献 > Recruitment of phosphorylated small heat shock protein Hsp27 to nuclear speckles without stress.

Recruitment of phosphorylated small heat shock protein Hsp27 to nuclear speckles without stress.

Abstract：

:During stress, the mammalian small heat shock protein Hsp27 enters cell nuclei. The present study examines the requirements for entry of Hsp27 into nuclei of normal rat kidney (NRK) renal epithelial cells, and for its interactions with specific nuclear structures. We find that phosphorylation of Hsp27 is necessary for the efficient entry into nuclei during heat shock but not sufficient for efficient nuclear entry under control conditions. We further report that Hsp27 is recruited to an RNAse sensitive fraction of SC35 positive nuclear speckles, but not other intranuclear structures, in response to heat shock. Intriguingly, Hsp27 phosphorylation, in the absence of stress, is sufficient for recruitment to speckles found in post-anaphase stage mitotic cells. Additionally, pseudophosphorylated Hsp27 fused to a nuclear localization peptide (NLS) is recruited to nuclear speckles in unstressed interphase cells, but wildtype and nonphosphorylatable Hsp27 NLS fusion proteins are not. The expression of NLS-Hsp27 mutants does not enhance colony forming abilities of cells subjected to severe heat shock, but does regulate nuclear speckle morphology. These data demonstrate that phosphorylation, but not stress, mediates Hsp27 recruitment to an RNAse soluble fraction of nuclear speckles and support a site-specific role for Hsp27 within the nucleus.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Bryantsev AL,Chechenova MB,Shelden EA

doi

10.1016/j.yexcr.2006.10.004

subject

Has Abstract

pub_date

2007-01-01 00:00:00

pages

195-209

issue

1

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(06)00422-8

journal_volume

313

pub_type

杂志文章

相关文献

EXPERIMENTAL CELL RESEARCH文献大全
  • miR-34a expands myeloid-derived suppressor cells via apoptosis inhibition.

    abstract：:Myeloid-derived suppressor cells (MDSCs) are a heterogeneous population and show significant expansion under pathological conditions. microRNA plays important roles in many biological processes, whether microRNAs have a function in the expansion of MDSCs is still not very clear. In this study, miR-34a overexpression c...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2014.04.010

    authors： Huang A,Zhang H,Chen S,Xia F,Yang Y,Dong F,Sun D,Xiong S,Zhang J

    更新日期：2014-08-15 00:00:00

  • A keratin of fetal skin is reexpressed in human keratinocytes transformed by SV40 virus or treated with the tumor promoter TPA.

    abstract：:SV40-transformation as well as treatment with tumor promoters produce alterations in morphology, differentiation and keratinization of human keratinocytes. Two cell lines of SV40-transformed keratinocytes and primary cultures of keratinocytes treated with the tumor promoter 12-O-tetradecanoyl phorbol-13-acetate (TPA) ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(84)90692-x

    authors： Darmon M,Delescluse C,Semat A,Bernard B,Bailly J,Prunieras M

    更新日期：1984-09-01 00:00:00

  • Differential regulation of protooncogenes c-jun and jun D expressions by protein tyrosine kinase, protein kinase C, and cyclic-AMP mitogenic pathways in dog primary thyrocytes: TSH and cyclic-AMP induce proliferation but downregulate C-jun expression.

    abstract：:The expressions of the protooncogenes c-jun and jun D have been investigated in dog thyrocytes in a primary culture whose proliferation is stimulated by three distinct intracellular signaling pathways (1) the thyrotropin (TSH) or forskolin-cyclic-AMP-mediated cascade; (2) the protein kinase C pathway activated by diac...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(91)90253-q

    authors： Reuse S,Pirson I,Dumont JE

    更新日期：1991-10-01 00:00:00

  • Immortalization of rat keratinocytes by transfection with polyomavirus large T gene.

    abstract：:Transfection of primary rat keratinocytes with the polyomavirus large T gene promotes the establishment of cell lines. The keratinocytes express the large T protein and can be continuously cultured in medium containing a low concentration of calcium. The immortalized keratinocytes retain the ability to differentiate w...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(88)90042-0

    authors： Bayley SA,Stones AJ,Smith CG

    更新日期：1988-07-01 00:00:00

  • Annexin VI is a mannose-6-phosphate-independent endocytic receptor for bovine β-glucuronidase.

    abstract：:Endocytosis and transport of bovine liver β-glucuronidase to lysosomes in human fibroblasts are mediated by two receptors: the well-characterized cation-independent mannose 6-phosphate receptor (IGF-II/Man6PR) and an IGF-II/Man6PR-independent receptor, which recognizes a Ser-Trp*-Ser sequence present on the ligand. Th...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2011.05.025

    authors： Ramírez-Mata A,Michalak C,Mendoza-Hernández G,León-Del-Río A,González-Noriega A

    更新日期：2011-10-01 00:00:00

  • EYA1 promotes tumor angiogenesis by activating the PI3K pathway in colorectal cancer.

    abstract：:Blood vessels are one of the major routes for the dissemination of cancer cells. Malignant tumors release growth factors such as vascular endothelial growth factor(VEGF) to induce angiogenesis, thereby promoting metastasis. Here, we report that The Drosophila Eyes Absent Homologue 1 (EYA1), which is overexpressed in c...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2018.02.028

    authors： Cai S,Cheng X,Liu Y,Lin Z,Zeng W,Yang C,Liu L,Chukwuebuka OA,Li W

    更新日期：2018-06-01 00:00:00

  • Characterization and physiological importance of a novel cell cycle regulated protein kinase in Xenopus laevis oocytes that phosphorylates cyclin B2.

    abstract：:We have partially purified a specific cyclin B2 kinase (cyk) from prophase oocytes of Xenopus laevis after an ATP-gamma-S activation step. Phosphopeptide analysis identified Ser53 as the major in vitro phosphorylation site for cyk in cyclin B2. Using a synthetic peptide derived from cyclin B2 encompassing Ser53 (cykti...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1996.3436

    authors： Derua R,Stevens I,Waelkens E,Fernandez A,Lamb N,Merlevede W,Goris J

    更新日期：1997-02-01 00:00:00

  • Human immunodeficiency virus type 1 Tat protein modulates cell cycle and apoptosis in Epstein-Barr virus-immortalized B cells.

    abstract：:Patients infected with human immunodeficiency virus type 1 (HIV-1) develop a spectrum of B cell lymphoproliferative disorders ranging from polyclonal B cell activation to B cell lymphomas. While a direct role of Epstein-Barr virus (EBV) is well recognized for most of these lesions, recent findings have suggested that ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2004.01.018

    authors： Colombrino E,Rossi E,Ballon G,Terrin L,Indraccolo S,Chieco-Bianchi L,De Rossi A

    更新日期：2004-05-01 00:00:00

  • Analysis of Tax-expressing cell lines generated from HTLV-I tax-transgenic mice: correlation between c-myc overexpression and neoplastic potential.

    abstract：:Human T-cell leukemia/lymphotropic virus type I (HTLV-I) infection causes a variety of human diseases, including adult T-cell leukemia/lymphoma. The viral transactivator Tax has been implicated as a key factor in the HTLV-I-induced transformation pathway. To investigate the components of this pathway, we derived fibro...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1998.4381

    authors： Saggioro D,D'agostino DM,Chieco-Bianchi L

    更新日期：1999-03-15 00:00:00

  • Role of Caenorhabditis elegans protein phosphatase type 1, CeGLC-7 beta, in metaphase to anaphase transition during embryonic development.

    abstract：:In Caenorhabditis elegans embryogenesis, phosphorylation events are critical to chromosomal changes. To investigate the dephosphorylation of chromosome behavior, we cloned and characterized the cDNA that encodes C. elegans protein phosphatase type 1 (CeGLC-7 beta), which is composed of 333 amino acids. CeGLC-7 beta po...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/s0014-4827(03)00157-5

    authors： Sassa T,Ueda-Ohba H,Kitamura K,Harada S,Hosono R

    更新日期：2003-07-15 00:00:00

  • Protein degradation and apoptotic death in lymphocytes during Fiv infection: activation of the ubiquitin-proteasome proteolytic system.

    abstract：:The movement of a cell through the sequential phases of apoptosis is accompanied by a progressive decrease in cell size with loss in protein mass. In lymphocytes from Hiv-infected persons, protein loss during apoptosis is due to increased protein degradation rather than decreased synthesis. To identify and characteriz...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1999.4410

    authors： Piedimonte G,Crinelli R,Della Salda L,Corsi D,Pennisi MG,Kramer L,Casabianca A,Sarli G,Bendinelli M,Marcato PS,Magnani M

    更新日期：1999-05-01 00:00:00

  • Advanced glycation endproducts produced by in vitro glycation of type I collagen modulate the functional and secretory behavior of dorsal root ganglion cells cultivated in two-dimensional system.

    abstract：:Advanced glycation end-products (AGEs) are proteins/lipids that are glycated upon sugar exposure and are often increased during inflammatory diseases such as osteoarthritis and neurodegenerative disorders. Here, we developed an extracellular matrix (ECM) using glycated type I collagen (ECM-GC), which produced similar ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2019.06.020

    authors： Bufalo MC,Almeida ME,Franca IA,Zambelli VO,Martins Sant'anna MB,Kimura LF,Giardini AC,Cury Y,Sampaio SC

    更新日期：2019-09-15 00:00:00

  • Differing calcification processes in cultured vascular smooth muscle cells and osteoblasts.

    abstract：:Arterial medial calcification (AMC) is the deposition of calcium phosphate mineral, often as hydroxyapatite, in the medial layer of the arteries. AMC shares some similarities to skeletal mineralisation and has been associated with the transdifferentiation of vascular smooth muscle cells (VSMCs) towards an osteoblast-l...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2019.04.020

    authors： Patel JJ,Bourne LE,Davies BK,Arnett TR,MacRae VE,Wheeler-Jones CP,Orriss IR

    更新日期：2019-07-01 00:00:00

  • Substrate modulation of morphology, growth, and tear protein production by cultured human lacrimal gland epithelial cells.

    abstract：:Human lacrimal gland biopsies were serially incubated in chelating and enzymatic solutions. Viable single cells were evaluated for outgrowth in growth factor enriched medium using the following culture substrates: Matrigel, Type I collagen gel with or without fibroblasts, and plastic. Each epithelial outgrowth was cha...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1995.1300

    authors： Yoshino K,Tseng SC,Pflugfelder SC

    更新日期：1995-09-01 00:00:00

  • Inhibition of tumor growth by a truncated and soluble form of melanotransferrin.

    abstract：:Melanotransferrin is a glycoprotein expressed at the cell membrane and secreted in the extracellular environment. Recombinant truncated form of membrane-bound melanotransferrin (sMTf) was reported to exert in vitro anti-angiogenic properties. Here we show that sMTf treatment leads to a 50% inhibition of neovasculariza...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2007.04.013

    authors： Rolland Y,Demeule M,Michaud-Levesque J,Béliveau R

    更新日期：2007-08-01 00:00:00

  • PDK1 promotes metastasis by inducing epithelial-mesenchymal transition in hypopharyngeal carcinoma via the Notch1 signaling pathway.

    abstract：:Hypopharyngeal squamous cell carcinoma (HSCC) is a relatively rare malignancy and has the worst prognosis among head and neck cancer. Metastasis is the major cause of poor prognosis in HSCC patients. In this study, we found that 3-phosphoinositide-dependent protein kinase 1 (PDK1 or PDPK1) was overexpressed in HSCC. T...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2019.111746

    authors： Jing P,Zhou S,Xu P,Cui P,Liu X,Liu X,Liu X,Wang H,Xu W

    更新日期：2020-01-15 00:00:00

  • Characterization of human bronchial epithelial cells immortalized by the E6 and E7 genes of human papillomavirus type 16.

    abstract：:We describe a novel human bronchial epithelial cell line immortalized after transfection with the E6 and E7 genes of human papillomavirus type 16. The established HBE4-E6/E7 cell line resembles morphologically the basal cells of the normal human bronchial epithelium and is nontumorigenic. A clonal isolate proved to be...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1994.1115

    authors： Viallet J,Liu C,Emond J,Tsao MS

    更新日期：1994-05-01 00:00:00

  • Kidins220/ARMS depletion is associated with the neural-to Schwann-like transition in a human neuroblastoma cell line model.

    abstract：:Peripheral neuroblastic tumors exist as a heterogeneous mixture of neuroblastic (N-type) cells and Schwannian stromal (S-type) cells. These stromal cells not only represent a differentiated and less aggressive fraction of the tumor, but also have properties that can influence the further differentiation of nearby mali...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2012.12.027

    authors： Rogers DA,Schor NF

    更新日期：2013-03-10 00:00:00

  • The nuclear factor kappa B (NF-κB) activation is required for phagocytosis of staphylococcus aureus by RAW 264.7 cells.

    abstract：:Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor which controls the expression of various genes involved in immune responses. However, it is not clear whether NF-κB activation is critical for phagocytosis when Staphylococcus aureus is the pathogen. Using oligonucleotide microarrays, we investigated ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2014.04.018

    authors： Zhu F,Yue W,Wang Y

    更新日期：2014-10-01 00:00:00

  • Dynamic temporal and cell type-specific expression of Wnt signaling components in the developing midbrain.

    abstract：:Wnt1 and -5a have been shown to modulate the proliferation and differentiation of midbrain dopaminergic (DA) neurons. However, it is not known whether other Wnts or which Frizzled (Fz) receptors are expressed in the developing midbrain. We found that 13 out of 19 Wnts, all 10 Fzs, and several intracellular Wnt signali...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2006.01.032

    authors： Rawal N,Castelo-Branco G,Sousa KM,Kele J,Kobayashi K,Okano H,Arenas E

    更新日期：2006-05-15 00:00:00

  • Fetal-adult phenotype transition, in terms of the serum dependency and growth factor requirements, of human skin fibroblast migration.

    abstract：:Since the migration of human fetal skin fibroblasts into a denuded area of a cell monolayer has been demonstrated to be serum-independent (fetal phenotype), whereas that of adult-donor skin fibroblasts is serum-dependent (adult phenotype), a study was carried out to determine at which life stage the fetal-adult phenot...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1995.1342

    authors： Kondo H,Yonezawa Y

    更新日期：1995-10-01 00:00:00

  • Identification of the GTPase-activating protein DEP domain containing 1B (DEPDC1B) as a transcriptional target of Pitx2.

    abstract：:Pitx2 is a bicoid-related homeobox transcription factor implicated in regulating left-right patterning and organogenesis. However, only a limited number of Pitx2 downstream target genes have been identified and characterized. Here we demonstrate that Pitx2 is a transcriptional repressor of DEP domain containing 1B (DE...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2015.02.008

    authors： Wu D,Zhu X,Jimenez-Cowell K,Mold AJ,Sollecito CC,Lombana N,Jiao M,Wei Q

    更新日期：2015-04-10 00:00:00

  • Nucleoporin35 is a novel microtubule associated protein functioning in oocyte meiotic spindle architecture.

    abstract：:Nucleoporins (Nups) are a large and diverse family of proteins that mediate nucleocytoplasmic transport at interphase of vertebrate cells. Nups also function in mitosis progression. However, whether Nups are involved in oocyte meiosis progression is still rarely known. In this study, we delineated the roles and regula...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2018.09.004

    authors： Chen F,Jiao XF,Zhang JY,Wu D,Ding ZM,Wang YS,Miao YL,Huo LJ

    更新日期：2018-10-15 00:00:00

  • Functional characterization of the spontaneously transformed human umbilical vein endothelial cell line ECV304: use in an in vitro model of angiogenesis.

    abstract：:To gain insight into the role of the endothelial cell during the pathophysiology of the angiogenic response, investigators have isolated micro- and macrovascular endothelial cells from a wide range of both animal and human vessels, including the umbilical vein. Human umbilical vein endothelial cells (HUVECs) isolated ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1996.0168

    authors： Hughes SE

    更新日期：1996-05-25 00:00:00

  • Modulation of membrane cholesterol levels: effects on endothelial cell function.

    abstract：:The endothelial cell lining of blood vessels is now recognized as an active interface between blood and the underlying tissue. Modulation of cholesterol levels in several cell types has resulted in altered cell function. We have removed cholesterol from the endothelial cell membrane and have observed corresponding alt...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(91)90548-9

    authors： Broadley C,Dawidowicz E,Chong PL,Hoover R

    更新日期：1991-03-01 00:00:00

  • The role of peroxisome proliferator-activated receptor-beta/delta in epidermal growth factor-induced HaCaT cell proliferation.

    abstract：:Epidermal growth factor (EGF) has been shown to be a potent mitogen for epidermal cells both in vitro and in vivo, thus contributing to the development of an organism. It has recently become clear that peroxisome proliferator-activated receptor-beta/delta (PPARbeta/delta) expression and activation is involved in the c...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2008.06.013

    authors： Liang P,Jiang B,Yang X,Xiao X,Huang X,Long J,Zhang P,Zhang M,Xiao M,Xie T,Huang X

    更新日期：2008-10-15 00:00:00

  • High glucose increases glomerular filtration barrier permeability by activating protein kinase G type Iα subunits in a Nox4-dependent manner.

    abstract：:Hyperglycemia is a primary factor that disturbs podocyte function in the glomerular filtration process; this disturbance leads to the development of diabetic nephropathy, and ultimately, renal failure. Podocyte function may also be altered by biological agents that modify protein kinase activity, including the cGMP-ac...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2013.09.005

    authors： Piwkowska A,Rogacka D,Audzeyenka I,Angielski S,Jankowski M

    更新日期：2014-01-01 00:00:00

  • In vitro replication and differentiation of normal human oral keratinocytes.

    abstract：:The replication kinetics and cytological changes of normal human oral keratinocytes (NHOK) isolated from the basal surface of oral epithelial sheet and cultured as dispersed cells in low (0.15 mM) Ca(2+) medium without serum were analyzed. Replicating NHOK were quantitated by cell count and identified by [(3)H]thymidi...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2000.4943

    authors： Kang MK,Bibb C,Baluda MA,Rey O,Park NH

    更新日期：2000-08-01 00:00:00

  • Nuclear translocation of a leukocyte elastase Inhibitor/Elastase complex during staurosporine-induced apoptosis: role in the generation of nuclear L-DNase II activity.

    abstract：:Using L1210 murine leukemia cells, we have previously shown that in response to treatment with drugs having different targets, apoptotic cell death occurs through at least two different signaling pathways. Here, we present evidence that nuclear extracts from staurosporine-treated cells elicit DNase II activity that is...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1999.4737

    authors： Belmokhtar CA,Torriglia A,Counis MF,Courtois Y,Jacquemin-Sablon A,Ségal-Bendirdjian E

    更新日期：2000-01-10 00:00:00

  • Mitotic segregation of the nucleolar ribosomal RNA in Physarum polycephalum.

    abstract：:In the naturally synchronous mitosis of the syncytial plasmodium of Physarum polycephalum, the nucleolus disintegrates in prophase, releasing a large amount of ribosomal RNA. Using biotinylated rDNA probes, we studied by high-resolution in situ hybridization the behavior of this nucleolar RNA throughout mitosis. Our r...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1993.1274

    authors： Pierron G,Puvion-Dutilleul F

    更新日期：1993-10-01 00:00:00