Abstract:
:Since the migration of human fetal skin fibroblasts into a denuded area of a cell monolayer has been demonstrated to be serum-independent (fetal phenotype), whereas that of adult-donor skin fibroblasts is serum-dependent (adult phenotype), a study was carried out to determine at which life stage the fetal-adult phenotype transition, in terms of serum dependency of human skin fibroblast migration, occurs. The results demonstrated that this phenotype transition occurred before birth, and, therefore, the fetal skin fibroblast phenotype differed from those of infant-, child-, adult-, and elderly-donor skin fibroblasts. But, the fetal-, child-, and adult-donor skin fibroblast phenotypes did not change during in vitro aging. On the other hand, the growth factor requirement for human skin fibroblast migration changed similarly to the serum dependency for fibroblast migration: the signal for fibroblast migration changed from basic fibroblast growth factor for the fetal phenotype to platelet-derived growth factor for the adult phenotype.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Kondo H,Yonezawa Ydoi
10.1006/excr.1995.1342subject
Has Abstractpub_date
1995-10-01 00:00:00pages
501-4issue
2eissn
0014-4827issn
1090-2422pii
S0014-4827(85)71342-0journal_volume
220pub_type
杂志文章abstract::Mixed lineage leukemia (MLL) arises from several KMT2A-gene chromosomal translocations. Shb gene deficiency has been found to exhibit pleiotropic effects in different models of leukemia, and consequently, this study aimed to investigate MLL-AF9-induced leukemia in Shb deficiency. Bone marrow cells from wild type and S...
journal_title:Experimental cell research
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journal_title:Experimental cell research
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doi:10.1016/0014-4827(89)90249-8
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journal_title:Experimental cell research
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abstract::Nogo-B/ASY has been characterized as a novel human apoptosis-inducing protein without any known apoptosis-related motifs. However, the validity of Nogo-B/ASY as a physiological apoptotic protein was recently questioned. In present research, we demonstrate that ASY overexpression contributes to ER stress and induces ap...
journal_title:Experimental cell research
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doi:10.1016/j.yexcr.2006.02.024
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abstract::When exponentially growing NHIK 3025 cells were shifted from medium containing 30% serum to medium containing 0.03% serum the rate of net protein accumulation was reduced due to both a reduction in the rate of protein synthesis and an increase in the rate of protein degradation. This change in growth conditions increa...
journal_title:Experimental cell research
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doi:10.1016/0014-4827(84)90788-2
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abstract::The 90-kDa heat shock protein, HSP90, is an abundant molecular chaperone which functions in cellular homeostasis in prokaryotes and eukaryotes. It is well known that HSP90 plays a critical and indispensable role in regulating cell growth through modulations of various signal transduction pathways, but its roles in cel...
journal_title:Experimental cell research
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abstract::UDP-glucose dehydrogenase (UGDH) catalyzes oxidation of UDP-glucose to yield UDP-glucuronic acid, a precursor of hyaluronic acid (HA) and other glycosaminoglycans (GAGs) in extracellular matrix. Although association of extracellular matrix with cell proliferation and migration has been well documented, the importance ...
journal_title:Experimental cell research
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2006.04.022
更新日期:2006-08-01 00:00:00
abstract::It has previously been shown by immunocytochemistry that the inactive X chromosome (Xi) in somatic cells of human and mouse females is marked by underacetylation of histone H4. It has been suggested that this may be important for transcriptional silencing of genes on Xi. We have now investigated X-inactivation in meio...
journal_title:Experimental cell research
pub_type: 杂志文章
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journal_title:Experimental cell research
pub_type: 杂志文章
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.2001.5254
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abstract::The extracellular matrix (ECM) contains important cues for tissue homeostasis and morphogenesis. The matricellular protein tenascin-C (TN-C) is overexpressed in remodeling tissues and cancer. In the present work, we studied the effect of different ECM-which exhibited a significant diversity in their TN-C content-in en...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2011.06.006
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2010.07.003
更新日期:2010-12-10 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1999.4446
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abstract::Integrin beta 4 subunit is present in association with alpha 6 chain on both normal and transformed epithelial cells. Recently alpha 6 beta 4 heterodimer was found on the endothelium of medium-sized blood vessels and on immature thymocytes. In this report we show, by Northern blotting, indirect immunofluorescence, imm...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1996.0271
更新日期:1996-09-15 00:00:00
abstract::The measurement of chromomycin A3/DAPI fluorescence ratios is shown to allow base content determinations in eu- and heterochromatic regions of interphase cell nuclei. The base content values obtained in chromocenters and euchromatin of Scilla sibirica agree with those measured earlier [12] on the band and non-band are...
journal_title:Experimental cell research
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doi:10.1016/0014-4827(83)90223-9
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journal_title:Experimental cell research
pub_type: 杂志文章
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1995.1101
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1999.4773
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(86)90607-5
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abstract::Heat shock proteins (HSPs) have molecular chaperone functions in protein biogenesis as well as cytoprotective functions against deleterious environmental stresses, and they work mainly inside of the cells. HSPs are usually induced in living cells that have been exposed to mild stresses or have recovered from severe st...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2005.07.014
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2014.08.013
更新日期:2015-01-15 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2006.07.018
更新日期:2006-11-01 00:00:00
abstract::In a recent screening for genes down regulated in replicatively senescent human umbilical vein endothelial cells (HUVECs), we have isolated the novel protein SNEV. Since then SNEV has proven as a multifaceted protein playing a role in pre-mRNA splicing, DNA repair, and the ubiquitin/proteosome system. Here, we report ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2005.11.025
更新日期:2006-04-01 00:00:00
abstract::Zinc is found in a variety of organisms and has been suggested to be essential for many cellular functions. We report here that zinc ions (Zn2+) have a specific and striking effect on cell differentiation in the simple eukaryote, Dictyostelium discoideum. In its asexual reproduction phase, solitary cells gather to for...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1994.1269
更新日期:1994-09-01 00:00:00
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journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/s0014-4827(03)00410-5
更新日期:2003-12-10 00:00:00
abstract::Calf aortic smooth muscle (CASM) cells cultured in vitro at high cell density (4 x 10(4) cells/cm2) on bacteriological petri dishes in the presence of serum pile up in clusters and create open spaces in the monolayer. This phenomenon is clearly visible 6 days after plating and is markedly enhanced by the addition of f...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(90)90216-w
更新日期:1990-01-01 00:00:00