Nuclear translocation of a leukocyte elastase Inhibitor/Elastase complex during staurosporine-induced apoptosis: role in the generation of nuclear L-DNase II activity.

abstract：:The lack of available, well characterized, established, domestic porcine cell lines hinders the advancement of porcine cellular immunology. A case of multicentric lymphoma was diagnosed in a market weight pig at the time of slaughter. Affected lymph nodes and spleen were collected and used for single cell isolation an...

journal_title：Experimental cell research

authors： Rahe MC,Dvorak CMT,Wiseman B,Martin D,Murtaugh MP

更新日期：2020-05-15 00:00:00

abstract：:Intermediate filaments (IFs), desmosomes, and their associates are built from multidomain proteins that form cytoskeletal scaffolds in the cytoplasm and the nucleus of vertebrate tissues. Mutations in more than 80 genes cause monogenic disorders that include severe skin fragility, myopathies, neurodegeneration, and pr...

journal_title：Experimental cell research

authors： Magin TM,Reichelt J,Hatzfeld M

更新日期：2004-11-15 00:00:00

abstract：:It is well established that the nucleus is endowed with enzymes that are involved in lipid-dependent signal transduction pathways. Diacylglycerol (DAG) is a fundamental lipid second messenger that is produced in the nucleus. Previous reports have shown that the nucleus contains diacylglycerol kinases (DGKs), i.e., the...

journal_title：Experimental cell research

authors： Tabellini G,Bortul R,Santi S,Riccio M,Baldini G,Cappellini A,Billi AM,Berezney R,Ruggeri A,Cocco L,Martelli AM

更新日期：2003-07-01 00:00:00

abstract：:Changes in the expression pattern of DNA polymerase beta gene during rat lung, brain, and testis development have been investigated. A decrease in the level of beta-pol mRNA was observed during postnatal development of lung and brain. By contrast, an almost 20-fold increase in the level of beta-pol mRNA was observed d...

journal_title：Experimental cell research

authors： Nowak R,Woszczynski M,Siedlecki JA

更新日期：1990-11-01 00:00:00

abstract：:The HRP-1 cell line is derived from normal rat placenta and appears morphologically similar to and retains characteristic expression of cellular markers of labyrinthine trophoblast cells. In this study, monolayers of HRP-1 cells grown on permeable supports were evaluated as a potential in vitro system to study trophob...

journal_title：Experimental cell research

authors： Shi F,Soares MJ,Avery M,Liu F,Zhang X,Audus KL

更新日期：1997-07-10 00:00:00

abstract：:RNAi mediated loss of Drp1 function changes mitochondrial morphology in cultured HeLa and HUVEC cells by shifting the balance of mitochondrial fission and fusion towards unopposed fusion. Over time, inhibition of Drp1 expression results in the formation of a highly branched mitochondrial network along with "bulge"-lik...

journal_title：Experimental cell research

authors： Möpert K,Hajek P,Frank S,Chen C,Kaufmann J,Santel A

更新日期：2009-08-01 00:00:00

abstract：:The purpose of the present study was to investigate the influence of the actin cytoskeleton on gap junctional intercellular communication (GJIC) in cultured astrocytes. This report describes an decrease of GJIC following microinjection of anti-actin antibodies or cytochalasin D treatment. Dye transfer of microinjected...

journal_title：Experimental cell research

authors： Theiss C,Meller K

更新日期：2002-12-10 00:00:00

abstract：:Skin extracellular matrix (ECM) molecules regulate a variety of cellular activities, including cell movement, which are central to wound healing and metastasis. Regulated cell movement is modulated by proteases and their associated molecules, including the serine proteases urinary-type plasminogen activator (uPA) and ...

journal_title：Experimental cell research

authors： Jones JM,Cohen RL,Chambers DA

更新日期：2002-11-01 00:00:00

abstract：:To gain insight into the role of the endothelial cell during the pathophysiology of the angiogenic response, investigators have isolated micro- and macrovascular endothelial cells from a wide range of both animal and human vessels, including the umbilical vein. Human umbilical vein endothelial cells (HUVECs) isolated ...

journal_title：Experimental cell research

authors： Hughes SE

更新日期：1996-05-25 00:00:00

abstract：:Endocytosis and transport of bovine liver β-glucuronidase to lysosomes in human fibroblasts are mediated by two receptors: the well-characterized cation-independent mannose 6-phosphate receptor (IGF-II/Man6PR) and an IGF-II/Man6PR-independent receptor, which recognizes a Ser-Trp*-Ser sequence present on the ligand. Th...

journal_title：Experimental cell research

authors： Ramírez-Mata A,Michalak C,Mendoza-Hernández G,León-Del-Río A,González-Noriega A

更新日期：2011-10-01 00:00:00

abstract：:Rabbit antisera prepared against embryonic chick liver cells have been characterized using cell cytotoxicity tests, antibody absorption tests, inhibition of cell-cell reaggregation and cell-aggregate adhesion assays. The sera contain antibodies which specifically block embryonic chick liver cell-cell aggregation with ...

journal_title：Experimental cell research

authors： Grady SR,Nielsen LD,McGuire EJ

更新日期：1982-11-01 00:00:00

abstract：:Extraocular muscles (EOMs) are categorized as skeletal muscles; however, emerging evidence indicates that their gene expression profile, metabolic characteristics and functional properties are significantly different from the prototypical members of this muscle class. Gene expression profiling of developing and adult ...

journal_title：Experimental cell research

authors： Moncman CL,Andrade FH

更新日期：2010-07-15 00:00:00

abstract：:The protein composition of the nuclear matrix of murine P19 embryonal carcinoma (EC) cells was compared with that of clonal derivatives of P19 EC differentiated in vitro, and with that of P19 EC cells induced to differentiate with retinoic acid (RA). Several major differences in nuclear matrix protein composition were...

journal_title：Experimental cell research

authors： Stuurman N,Van Driel R,De Jong L,Meijne AM,Van Renswoude J

更新日期：1989-02-01 00:00:00

abstract：:The MUC1 mucin is a large membrane-tethered glycoprotein that shows differential expression in many adenocarcinomas, where it contributes to their invasive and metastatic properties. We previously identified DNase I hypersensitive sites at -750 and -250 bp in the human MUC1 gene promoter and showed concordance between...

journal_title：Experimental cell research

authors： Shiraga T,Winpenny JP,Carter EJ,McCarthy VA,Hollingsworth MA,Harris A

更新日期：2005-08-01 00:00:00

abstract：:We report that a specific inhibitor of types 1 and 2A phosphatases, okadaic acid (OA), induces germinal vesicle break down (GVBD) and chromosome condensation when microinjected into denuded mouse oocytes maintained in prophase block by analogs of cAMP, inhibitors of phosphodiesterase, or a tumor-promoting phorbol este...

journal_title：Experimental cell research

authors： Gavin AC,Tsukitani Y,Schorderet-Slatkine S

更新日期：1991-01-01 00:00:00

abstract：:In many systems, events participating in cell division are controlled by intracellular pH (pHi). In Xenopus eggs, fertilization is accompanied by an increase in pHi which occurs concomitantly with an increase in protein synthesis and a reinitiation of DNA synthesis, leading the embryo to cell division. In this paper, ...

journal_title：Experimental cell research

authors： Grandin N,Charbonneau M

更新日期：1989-12-01 00:00:00

abstract：:Cell growth is controlled by the complex interactions of both positive and negative growth modulators. Studies were performed to directly compare the growth inhibitory properties of TGF-beta 1 and CeReS-18, a novel cell surface sialoglycopeptide growth inhibitor. Growth inhibition by CeReS-18 and that by TGF-beta 1 sh...

journal_title：Experimental cell research

authors： Betz NA,Fattaey HK,Johnson TC

更新日期：1996-08-25 00:00:00

abstract：:The tumor-associated immune response is governed by the signalling events of various regulatory molecules, one of which is the cytotoxic T lymphocyte-associated antigen 4 (CTLA-4). In conventional T cells, CTLA-4 could outcompete CD28 in binding to CD80/86 but does not produce a co-stimulatory signal, resulting in T c...

journal_title：Experimental cell research

authors： Lang C,Wang J,Chen L

更新日期：2017-11-15 00:00:00

abstract：:Polycystin-2 (PC2, TRPP2) is a nonselective cation channel whose dysfunction is associated with the onset of autosomal dominant polycystic kidney disease (ADPKD). PC2 contributes to Ca2+ transport and cell signaling in renal epithelia and other tissues. Little is known however, as to the external Ca2+ regulation of PC...

journal_title：Experimental cell research

authors： Dai XQ,Perez PL,Soria G,Scarinci N,Smoler M,Morsucci DC,Suzuki K,Cantero MDR,Cantiello HF

更新日期：2017-01-01 00:00:00

abstract：:Analyses of mice with targeted deletions in the genes for alpha3 and beta1 integrin suggest that the alpha3beta1 integrin heterodimer likely determines the organization of the extracellular matrix within the basement membrane of skin. Here we tested this hypothesis using keratinocytes derived from alpha3 integrin-null...

journal_title：Experimental cell research

authors： deHart GW,Healy KE,Jones JC

更新日期：2003-02-01 00:00:00

abstract：:Fenretinide is an effective inducer of apoptosis in many malignancies but its precise mechanism(s) of action in the induction of apoptosis in neuroblastoma is unclear. To characterize fenretinide-induced apoptosis, neuroblastoma cell lines were treated with fenretinide and flow cytometry was used to measure apoptosis,...

journal_title：Experimental cell research

authors： Lovat PE,Ranalli M,Annichiarrico-Petruzzelli M,Bernassola F,Piacentini M,Malcolm AJ,Pearson AD,Melino G,Redfern CP

更新日期：2000-10-10 00:00:00

abstract：:We studied the expression of FREK (fibroblast growth factor receptor-like embryonic kinase), a new receptor recently cloned from quail embryo, during the differentiation of skeletal muscle satellite cells and epiphyseal growth-plate chondrocytes. Although FREK mRNA was expressed in both cell types, satellite cells exp...

journal_title：Experimental cell research

authors： Halevy O,Monsonego E,Marcelle C,Hodik V,Mett A,Pines M

更新日期：1994-06-01 00:00:00

abstract：:Epidermal growth factor (EGF) has been shown to be a potent mitogen for epidermal cells both in vitro and in vivo, thus contributing to the development of an organism. It has recently become clear that peroxisome proliferator-activated receptor-beta/delta (PPARbeta/delta) expression and activation is involved in the c...

journal_title：Experimental cell research

authors： Liang P,Jiang B,Yang X,Xiao X,Huang X,Long J,Zhang P,Zhang M,Xiao M,Xie T,Huang X

更新日期：2008-10-15 00:00:00

abstract：:Treatment with ionic detergents of nuclei isolated from various continuously growing cell lines generally yields chromatin samples of high viscosity. Extensive treatment with nuclease-free proteinase K or pronase solubilized the viscous lysates with > 90% of the DNA migrating at approximately 50 kb. Freshly prepared h...

journal_title：Experimental cell research

authors： Szabó G Jr

更新日期：1995-12-01 00:00:00

abstract：:Cleavage of specific protein subsets is a key event in the execution of apoptosis. Protein degradation may serve for the structural alterations that result in cell self-destruction, but it may also function as a switch in the decisions between apoptosis and necrosis or apoptosis and cell proliferation. Here, we show t...

journal_title：Experimental cell research

authors： Schwab BL,Leist M,Knippers R,Nicotera P

更新日期：1998-02-01 00:00:00

abstract：:Cross-linking of CD44 in vitro promotes chemokinesis and actin-based dendrite formation in T and B cells. However, the mechanisms by which the adhesion molecule CD44 induces cytoskeleton activation in lymphocytes are still poorly understood. In this study, we have investigated whether myosin isoforms are involved in C...

journal_title：Experimental cell research

authors： Sumoza-Toledo A,Gillespie PG,Romero-Ramirez H,Ferreira-Ishikawa HC,Larson RE,Santos-Argumedo L

更新日期：2006-10-15 00:00:00

abstract：:Histidine-rich glycoprotein (HRGP) is an abundant heparin-binding plasma protein. We have shown that a fragment released from the central histidine/proline-rich (His/Pro-rich) domain of HRGP blocks endothelial cell migration in vitro and vascularization and growth of murine fibrosarcoma in vivo. The minimal active HRG...

journal_title：Experimental cell research

authors： Lee C,Dixelius J,Thulin A,Kawamura H,Claesson-Welsh L,Olsson AK

更新日期：2006-08-01 00:00:00

abstract：:During mitosis there is an abrupt inhibition of a wide range of membrane functions, including fluid-phase and adsorptive pinocytosis. We have used cell hybrids formed between mitotic and interphase cells to approach the mechanism of this inhibition. We report that fluid pinocytosis is reactivated in the mitotic partne...

journal_title：Experimental cell research

authors： Oliver JM,Seagrave JC

更新日期：1987-08-01 00:00:00

abstract：:Mps-one-binder (Mob) proteins play a crucial role in yeast cytokinesis. After cloning two Mob1-like genes, MsMob1-A and MsMob1-B from alfalfa (Medicago sativa L.) we show that, although they are constitutively expressed in roots, stems, leaves, flowers and pods, their transcripts and proteins are mostly produced in ac...

journal_title：Experimental cell research

authors： Citterio S,Piatti S,Albertini E,Aina R,Varotto S,Barcaccia G

更新日期：2006-04-15 00:00:00

abstract：:The laminins consist of at least 11 polypeptides (5 alpha-chains, 3 beta-chains, and 3 gamma-chains) specific to basement membranes. Here we investigate the biological activity associated with the G domain of the newly identified laminin alpha5-chain using 113 overlapping synthetic peptides (positions 2679-3635). Usin...

journal_title：Experimental cell research

authors： Makino M,Okazaki I,Kasai S,Nishi N,Bougaeva M,Weeks BS,Otaka A,Nielsen PK,Yamada Y,Nomizu M

更新日期：2002-07-01 00:00:00