Abstract：
:The HRP-1 cell line is derived from normal rat placenta and appears morphologically similar to and retains characteristic expression of cellular markers of labyrinthine trophoblast cells. In this study, monolayers of HRP-1 cells grown on permeable supports were evaluated as a potential in vitro system to study trophoblast transport and metabolism. The cell line was shown to express and retain functional activity of the predominant placental cytochrome P450 isozyme, CYP1A1. Additionally, the HRP-1 cells retain functional activity of angiotensin I converting enzyme and carboxypeptidase N-like enzyme, peptidases characteristic of the trophoblast. The permeation of several hydrophilic, inert markers across the HRP-1 monolayers was observed to be dependent on effective molecular size and to be passive in nature. Functional asymmetry of the HRP-1 cells was illustrated by the predominant permeation of linoleic acid in the apical-to-basolateral direction across the monolayers. Transferrin passage across HRP-1 monolayers was concentration-dependent, was bidirectional, and could be inhibited by unlabeled transferrin, features typical of the trophoblast transport system for transferrin. Collectively, these properties suggest that the HRP-1 cell line may provide a useful tool for evaluating some of the permeability and metabolic properties of the trophoblast.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Shi F,Soares MJ,Avery M,Liu F,Zhang X,Audus KLdoi
10.1006/excr.1997.3603subject
Has Abstractpub_date
1997-07-10 00:00:00pages
147-55issue
1eissn
0014-4827issn
1090-2422pii
S0014-4827(97)93603-Xjournal_volume
234pub_type
杂志文章abstract：:Extraocular muscles (EOMs) are categorized as skeletal muscles; however, emerging evidence indicates that their gene expression profile, metabolic characteristics and functional properties are significantly different from the prototypical members of this muscle class. Gene expression profiling of developing and adult ...
journal_title：Experimental cell research
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journal_title：Experimental cell research
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journal_title：Experimental cell research
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journal_title：Experimental cell research
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更新日期：1989-03-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2013.12.021
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2001.5446
更新日期：2002-02-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1996.0071
更新日期：1996-02-25 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章,评审
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更新日期：2016-08-01 00:00:00
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journal_title：Experimental cell research
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doi：10.1006/excr.2001.5377
更新日期：2001-12-10 00:00:00
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journal_title：Experimental cell research
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1995.1024
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journal_title：Experimental cell research
pub_type： 杂志文章
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