Abstract：
:This study investigates how epithelial cells moving together function to coordinate their collective movement to repair a wound. Using a lens ex vivo mock cataract surgery model we show that region-specific reorganization of cell-cell junctions, cytoskeletal networks and myosin function along apical and basal domains of an epithelium mediates the process of collective migration. An apical junctional complex composed of N-cadherin/ZO-1/myosin II linked to a cortical actin cytoskeleton network maintains integrity of the tissue during the healing process. These cells' basal domains often preceded their apical domains in the direction of movement, where an atypical N-cadherin/ZO-1 junction, linked to an actin stress fiber network rich in phosphomyosin, was prominent in cryptic lamellipodia. These junctions joined the protruding forward-moving lamellipodia to the back end of the cell moving directly in front of it. These were the only junctions detected in cryptic lamellipodia of lens epithelia migrating in response to wounding that could transmit the protrusive forces that drive collective movement. Both integrity of the epithelium and ability to effectively heal the wound was found to depend on myosin mechanical cues.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Menko AS,Bleaken BM,Walker JLdoi
10.1016/j.yexcr.2013.12.021subject
Has Abstractpub_date
2014-03-10 00:00:00pages
133-48issue
1eissn
0014-4827issn
1090-2422pii
S0014-4827(13)00556-9journal_volume
322pub_type
杂志文章abstract：:Hemidesmosomes are cell-to-matrix adhesion complexes anchoring keratinocytes to basement membranes. For the first time, we present a method to prepare a fraction from human cultured cells that are highly enriched in hemidesmosomal proteins. Using DJM-1 cells derived from human squamous cell carcinoma, accumulation of ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2014.04.002
更新日期：2014-06-10 00:00:00
abstract：:The androgen receptor (AR) plays critical roles in human prostate carcinoma progression and transformation. However, the activation of AR is regulated by co-regulators. MEIS1 protein, the homeodomain transcription factor, exhibited a decreased level in poor-prognosis prostate tumors. In this study, we investigated a p...
journal_title：Experimental cell research
pub_type： 杂志文章
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journal_title：Experimental cell research
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更新日期：2009-04-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2015.04.006
更新日期：2015-06-10 00:00:00
abstract：:Glycosaminoglycans (heparins, dermatan sulfate, chondroitin sulfate) with different structures and physicochemical properties were evaluated for their capacity to influence proliferation and differentiation of U-937 cell line. The contrasting and specific effects of glycosaminoglycans (depending on their structures an...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1994.1323
更新日期：1994-11-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(91)90105-4
更新日期：1991-04-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：1991-01-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(89)90198-5
更新日期：1989-03-01 00:00:00
abstract：:Previous studies have demonstrated that treatment of fibroblasts from confluent, density-inhibited cultures with 50 mM KCl solution led to the onset of DNA replication. In the present study we show that such treatment can induce aphidicolin-sensitive DNA replication in differentiated nondividing cells: dorsal root gan...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1993.1296
更新日期：1993-11-01 00:00:00
abstract：:Human lacrimal gland biopsies were serially incubated in chelating and enzymatic solutions. Viable single cells were evaluated for outgrowth in growth factor enriched medium using the following culture substrates: Matrigel, Type I collagen gel with or without fibroblasts, and plastic. Each epithelial outgrowth was cha...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1995.1300
更新日期：1995-09-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1994.1012
更新日期：1994-01-01 00:00:00
abstract：:N-methyl-d-aspartate (NMDA) subtype of glutamate receptors plays an important role during brain development and receptor activation has been shown to promote neuronal survival. The mechanisms by which NMDA increases neuronal survival are not fully understood. Here, we show that treatment with low concentrations of NMD...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2003.12.026
更新日期：2004-05-01 00:00:00
abstract：:Previous studies have shown that calpains are autolytically cleaved during the disease process of mdx dystrophy, a mouse model for Duchenne muscular dystrophy, indicating that calpains may be activated and play a role in proteolysis that occurs in muscular dystrophy (J. Biol. Chem. 270(18), 10909-10914, 1995). In the ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1996.0227
更新日期：1996-08-01 00:00:00
abstract：:Intracellular signaling pathways present targets for pharmacological agents with potential for treatment of neoplastic diseases, with some disease remissions already recorded. However, cellular compensatory mechanisms usually negate the initial success. For instance, attempts to interrupt aberrant signaling downstream...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2014.10.003
更新日期：2015-01-01 00:00:00
abstract：:Shp-2, a widely expressed cytoplasmic tyrosine phosphatase with two src-homology 2 (SH2) domains, has received much attention in the signal transduction field recently. Significant progress has been made in understanding the structure and function of this phosphatase, together with its Drosophila homologue, Corkscrew,...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1006/excr.1999.4668
更新日期：1999-11-25 00:00:00
abstract：:The Autoimmune Regulator (AIRE) is a regulator of transcription in the thymic medulla, where it controls the expression of a large set of peripheral-tissue specific genes. AIRE interacts with the transcriptional coactivator and acetyltransferase CBP and synergistically cooperates with it in transcriptional activation....
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2012.04.013
更新日期：2012-08-15 00:00:00
abstract：:Although 12-day-old chick embryo retinal pigment epithelial (RPE) cells in situ do not express the proliferating cell nuclear antigen (PCNA) which is known to function as an auxiliary protein of DNA polymerase delta, they do so when cultured on glass. Conversely, PCNA was not expressed by RPE cells of the same age mai...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(91)90378-8
更新日期：1991-08-01 00:00:00
abstract：:Cisplatin resistance in cancer cells is due to a pleiotropic phenotype transition that allows cells to resist cell death. miRNAs have been shown to be reliable markers of phenotype, critical in cell differentiation, and dysregulated in cancer and other pathologies. Here we investigate the influence of miRNA on cisplat...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2012.10.012
更新日期：2013-02-15 00:00:00
abstract：:Using hybridization in situ with a ribo-probe recognizing transcripts of the chicken alpha A globin gene, we show here that in proliferating AEV-transformed erythroblasts this gene is strongly transcribed, but the corresponding transcripts are retained in the nuclei. Most surprisingly, this globin RNA accumulates in t...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2001.5332
更新日期：2001-11-01 00:00:00
abstract：:Numerous factors that promote movement of macromolecules in and out of the nucleus have now been identified. These include both soluble cytoplasmic and nucleoplasmic proteins and proteins of the nuclear pore complex (NPC). Genetic analyses of the nuclear transport process in the model organism, the budding yeast Sacch...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1006/excr.1996.0362
更新日期：1996-12-15 00:00:00
abstract：:Myostatin, a Transforming Growth Factor-beta (TGF-beta) super-family member, has previously been shown to negatively regulate satellite cell activation and self-renewal. However, to date the mechanism behind Myostatin function in satellite cell biology is not known. Here we show that Myostatin signals via a Pax7-depen...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2007.09.012
更新日期：2008-01-15 00:00:00
abstract：:Mps-one-binder (Mob) proteins play a crucial role in yeast cytokinesis. After cloning two Mob1-like genes, MsMob1-A and MsMob1-B from alfalfa (Medicago sativa L.) we show that, although they are constitutively expressed in roots, stems, leaves, flowers and pods, their transcripts and proteins are mostly produced in ac...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2005.12.032
更新日期：2006-04-15 00:00:00
abstract：:Primary cultures of renal rabbit proximal tubule cells were initiated from a pure suspension of proximal tubule fragments. Proximal tubule cells were grown in a hormone-supplemented, serum-free medium containing low concentrations of antibiotics. Confluent monolayers exhibited multicellular dome formation, indicating ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(91)90123-c
更新日期：1991-05-01 00:00:00
abstract：:A sufficient supply molecular oxygen is essential for the maintenance of physiologic metabolism and bioenergetic homeostasis for most metazoans. For this reason, mechanisms have evolved for eukaryotic cells to adapt to conditions where oxygen demand exceeds supply (hypoxia). These mechanisms rely on the modification o...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2017.02.024
更新日期：2017-07-15 00:00:00
abstract：:Recently, we isolated 4 distinct kinds of single chain antibody against human EGF receptor (EGFR) after screening the Keio phage display scFv library by using two methods of target-guided proximity labeling. In the current study, these monovalent scFv antibodies were converted to bivalent IgGs of humanized forms (hIgG...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2013.03.002
更新日期：2013-05-01 00:00:00
abstract：:Human T-cell leukemia/lymphotropic virus type I (HTLV-I) infection causes a variety of human diseases, including adult T-cell leukemia/lymphoma. The viral transactivator Tax has been implicated as a key factor in the HTLV-I-induced transformation pathway. To investigate the components of this pathway, we derived fibro...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1998.4381
更新日期：1999-03-15 00:00:00
abstract：:Centrosomes, the main microtubule-organizing centers (MTOCs) in most animal cells, are important for many cellular activities such as assembly of the mitotic spindle, establishment of cell polarity, and cell movement. In nuclear transfer (NT), MTOCs that are located at the poles of the meiotic spindle are removed from...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2005.02.009
更新日期：2005-05-15 00:00:00
abstract：:Translation is mediated partly by regulation of free eukaryotic initiation factor 4E (eIF4E) levels through PI3K-Akt-mTOR signaling. Cancer cells treated with the plant-derived perillyl alcohol (POH) or the mechanistic target of rapamycin (mTOR) inhibitor rapamycin dephosphorylate eIF4E-binding protein (4E-BP1) and at...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2013.05.029
更新日期：2013-08-01 00:00:00
abstract：:The osteoblast-like MC3T3-E1 cell line provides an excellent in vitro model of bone development. This system undergoes three orderly time-dependent phases characterized by proliferating preosteoblasts, matrix accumulation by postmitotic differentiating osteoblasts, and mineralization of the matrix, which results in th...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2000.4879
更新日期：2000-05-25 00:00:00
abstract：:It has been known for at least 20 years that growth factors induce the internalization of cognate receptor tyrosine kinases (RTKs). The internalized receptors are then sorted to lysosomes or recycled to the cell surface. More recently, data have been published to indicate other intracellular destinations for the inter...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2008.09.027
更新日期：2009-05-15 00:00:00
