Abstract:
:Using hybridization in situ with a ribo-probe recognizing transcripts of the chicken alpha A globin gene, we show here that in proliferating AEV-transformed erythroblasts this gene is strongly transcribed, but the corresponding transcripts are retained in the nuclei. Most surprisingly, this globin RNA accumulates in the perinucleolar areas in a pattern never observed before. Upon induction of cells to differentiate, leading to productive expression of the hemoglobins, the transcripts of the alpha A globin gene were found for the most part in the cytoplasm. In the nuclei of differentiated cells, the globin RNA is concentrated in one or two specific spots, which are likely to represent the "processing centers" (PCs) of the globin RNA. The results presented indicate that posttranscriptional steps of regulation involving in particular the perinuclear areas are of major importance for erythroid differentiation.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Iarovaia O,Razin SV,Linares-Cruz G,Sjakste N,Scherrer Kdoi
10.1006/excr.2001.5332keywords:
subject
Has Abstractpub_date
2001-11-01 00:00:00pages
159-65issue
2eissn
0014-4827issn
1090-2422pii
S0014-4827(01)95332-7journal_volume
270pub_type
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