当前位置： SCI文献检索 > EXPERIMENTAL CELL RESEARCH期刊下所有文献 > Biochemical, functional, and morphological characterization of a primary culture of rabbit proximal tubule cells.

Biochemical, functional, and morphological characterization of a primary culture of rabbit proximal tubule cells.

Abstract：

:Primary cultures of renal rabbit proximal tubule cells were initiated from a pure suspension of proximal tubule fragments. Proximal tubule cells were grown in a hormone-supplemented, serum-free medium containing low concentrations of antibiotics. Confluent monolayers exhibited multicellular dome formation, indicating the presence of transepithelial solute and water transport. Ultrastructural examination revealed a monolayer of polarized epithelial cells with tight junctions and sparse membraneous microvilli facing the culture medium. Time course biochemical characterization was performed using a palette of 12 enzymes, representative of important metabolic functions or pathways. Brush-border-associated enzymes (gamma-glutamyl transpeptidase and alanine aminopeptidase) were moderately reduced throughout the culture whereas alkaline phosphatase was markedly decreased at confluency. Mitochondrial and lysosomal marker enzymes were well preserved over the culture period. Glutathione-S-transferase activity remained stable during the 16-day culture period investigated. Glycolysis enzyme activities (lactate dehydrogenase and hexokinase) were enhanced, as a function of culture age. Na(+)-K(+)-ATPase activity rise was concomitant with the increase of glycolysis marker enzymes. In contrast, the gluconeogenesis marker enzyme, glucose-6-phosphatase, fell dramatically to reach a low level equivalent to 4% of the activity measured in isolated proximal tubules. Primary cultures exhibited several differentiated functions of the proximal tubule cell: (a) PTH alone was able to induce a significant stimulation of adenylate cyclase activity, unlike isoproterenol, thyrocalcitonin, and arginine vasopressin, and (b) sodium-dependent alpha-methylglucoside (AMG) transport was detected. This AMG uptake was selectively inhibited by phlorizin (5 X 10(-3) M), which is a competitive inhibitor of glucose uptake at the apical membrane. Complete characterization made it possible to investigate hitherto unexplored aspects of in vitro cultured proximal tubule cells. This primary culture model could provide a useful and reliable tool to investigate in vitro renal proximal tubule function, under normal conditions or after a drug-induced toxicity.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Toutain H,Vauclin-Jacques N,Fillastre JP,Morin JP

doi

10.1016/0014-4827(91)90123-c

subject

Has Abstract

pub_date

1991-05-01 00:00:00

pages

9-18

issue

1

eissn

0014-4827

issn

1090-2422

pii

0014-4827(91)90123-C

journal_volume

194

pub_type

杂志文章

相关文献

EXPERIMENTAL CELL RESEARCH文献大全
  • Differential regulation of X-chromosome-linked inhibitor of apoptosis protein (XIAP) and caspase-3 by NMDA in developing hippocampal neurons; involvement of the mitochondrial pathway in NMDA-mediated neuronal survival.

    abstract：:N-methyl-d-aspartate (NMDA) subtype of glutamate receptors plays an important role during brain development and receptor activation has been shown to promote neuronal survival. The mechanisms by which NMDA increases neuronal survival are not fully understood. Here, we show that treatment with low concentrations of NMD...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2003.12.026

    authors： Korhonen L,Näpänkangas U,Steen H,Chen Y,Martinez R,Lindholm D

    更新日期：2004-05-01 00:00:00

  • Isolation of a hemidesmosome-rich fraction from a human squamous cell carcinoma cell line.

    abstract：:Hemidesmosomes are cell-to-matrix adhesion complexes anchoring keratinocytes to basement membranes. For the first time, we present a method to prepare a fraction from human cultured cells that are highly enriched in hemidesmosomal proteins. Using DJM-1 cells derived from human squamous cell carcinoma, accumulation of ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2014.04.002

    authors： Hirako Y,Yonemoto Y,Yamauchi T,Nishizawa Y,Kawamoto Y,Owaribe K

    更新日期：2014-06-10 00:00:00

  • Inflammation-driven colon neoplasmatogenesis in uPA-deficient mice is associated with an increased expression of Runx transcriptional regulators.

    abstract：:Deregulation of the bone morphogenetic protein (BMP) pathway has been documented in colorectal cancer (CRC). Previously, we investigated possible associations between urokinase-type plasminogen activator (uPA) deficiency and expression of extracellular constituents of BMP signaling in a newly developed mouse model of ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2017.10.025

    authors： Afaloniati H,Karagiannis GS,Hardas A,Poutahidis T,Angelopoulou K

    更新日期：2017-12-15 00:00:00

  • Uptake and metabolism of nucleosides by embryos of the sea urchin Strongylocentrotus purpuratus.

    abstract：:Uptake and metabolism of thymidine and adenosine have been studied in embryos of the sea urchin Strongylocentrotus purpuratus. Uptake of these nucleosides is found to be mutually competitive, with the Km for uptake of thymidine similar to its Ki for inhibition of adenosine uptake and vice versa. The metabolic studies ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(87)90411-3

    authors： Schneider EG,Whitten DJ

    更新日期：1987-01-01 00:00:00

  • Cell size of proliferating plant cells increases with temperature: implications in the control of cell division.

    abstract：:As chemical reactions related to the regulation of cell proliferation are governed by availability, amount, and concentration of relevant molecules, it has been suggested that cell size is an important factor in the control of cell cycle. We have measured the size of proliferating cells of Allium cepa roots in which g...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(89)90056-6

    authors： Cuadrado A,Navarrete MH,Canovas JL

    更新日期：1989-11-01 00:00:00

  • Cytoskeletal binding proteins distinguish cultured dental follicle cells and periodontal ligament cells.

    abstract：:Human dental follicle cells (DFCs) and periodontal ligament cells (PDLCs) derived from the ectomesenchymal tissue, have been shown to exhibit stem/progenitor cell properties and the ability to induce tissue regeneration. Stem cells in dental follicle differentiate into cementoblasts, periodontal ligament fibroblasts a...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2015.12.011

    authors： Li J,Li H,Tian Y,Yang Y,Chen G,Guo W,Tian W

    更新日期：2016-07-01 00:00:00

  • Intronic miR-932 targets the coding region of its host gene, Drosophila neuroligin2.

    abstract：:Despite great progress for two decades in microRNAs (miRNAs), the direct regulation of host gene by intragenic (mostly intronic) miRNA is conceptually plausible but evidence-limited. Here, we report that intronic miR-932 could target its host gene via binding with coding sequence (CDS) region rather than regular 3'UTR...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2016.01.017

    authors： Qian J,Tu R,Yuan L,Xie W

    更新日期：2016-06-10 00:00:00

  • Abnormal behavior of the yolk centrosomes during early embryogenesis of Drosophila melanogaster.

    abstract：:After the 10th nuclear cycle the yolk centrosomes follow an irregular pathway. Unlike the somatic centrosomes, which move to the opposite poles of the nuclei to form the bipolar spindles, the yolk centrosomes remain as pairs at one pole of the yolk nuclei or shift feebly and nucleate irregular spindles, most of which ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(91)90151-j

    authors： Callaini G,Dallai R

    更新日期：1991-01-01 00:00:00

  • The effects of activin A on the migration of human breast cancer cells and neutrophils and their migratory interaction.

    abstract：:Activin A belongs to the superfamily of transforming growth factor beta (TGFβ) and is a critical regulatory cytokine in breast cancer and inflammation. However, the role of activin A in migration of breast cancer cells and immune cells was not well characterized. Here, a microfluidic device was used to examine the eff...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2017.05.003

    authors： Xie D,Liu Z,Wu J,Feng W,Yang K,Deng J,Tian G,Santos S,Cui X,Lin F

    更新日期：2017-08-01 00:00:00

  • Alpha-factor inhibition of the rate of cell passage through the "start" step of cell division in Saccharomyces cerevisiae yeast: estimation of the division delay per alpha-factor.receptor complex.

    abstract：:A highly sensitive, kinetically unambiguous assay for alpha-factor-induced delay of cell passage through the "start" step of cell division in yeast is presented. The assay employs perfusion with periodic microscopy to monitor the bud emergence kinetics on the 20% of cells within an exponentially growing population whi...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(87)90173-x

    authors： Moore SA

    更新日期：1987-08-01 00:00:00

  • Anchorage-independent culture of bovine granulosa cells: the effects of basic fibroblast growth factor and dibutyryl cAMP on cell division and differentiation.

    abstract：:During ovarian folliculogenesis granulosa cells divide while in contact with stromal cells and other granulosa cells. Following ovulation, however, they cease dividing and differentiate into large luteal cells. When cultured in monolayer, granulosa cells spontaneously differentiate into luteal cells, thus confounding ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1994.1084

    authors： Lavranos TC,Rodgers HF,Bertoncello I,Rodgers RJ

    更新日期：1994-04-01 00:00:00

  • Differing calcification processes in cultured vascular smooth muscle cells and osteoblasts.

    abstract：:Arterial medial calcification (AMC) is the deposition of calcium phosphate mineral, often as hydroxyapatite, in the medial layer of the arteries. AMC shares some similarities to skeletal mineralisation and has been associated with the transdifferentiation of vascular smooth muscle cells (VSMCs) towards an osteoblast-l...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2019.04.020

    authors： Patel JJ,Bourne LE,Davies BK,Arnett TR,MacRae VE,Wheeler-Jones CP,Orriss IR

    更新日期：2019-07-01 00:00:00

  • Expression of N-cadherin and alkaline phosphatase in chick limb bud mesenchymal cells: regulation by 1,25-dihydroxyvitamin D3 or TGF-beta 1.

    abstract：:When mesenchymal cells from the early chick limb bud (stage 23-24) are plated at high cell density they spontaneously undergo chondrogenesis implicating extensive cell-to-cell interactions. In the past it has been shown that TGF-beta and vitamin D can influence this process and can stimulate chondrogenesis. Given the ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1994.1220

    authors： Tsonis PA,Del Rio-Tsonis K,Millan JL,Wheelock MJ

    更新日期：1994-08-01 00:00:00

  • Intracellular protein delivery activity of peptides derived from insulin-like growth factor binding proteins 3 and 5.

    abstract：:Insulin-like growth factor binding proteins (IGFBPs) have various IGF-independent cellular activities, including receptor-independent cellular uptake followed by transcriptional regulation, although mechanisms of cellular entry remain unclear. Herein, we focused on their receptor-independent cellular entry mechanism i...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2008.05.008

    authors： Goda N,Tenno T,Inomata K,Shirakawa M,Tanaka T,Hiroaki H

    更新日期：2008-08-01 00:00:00

  • Cell surface glycoproteins of CHO cells. I. Internalization and rapid recycling.

    abstract：:The major cell surface proteins of Chinese hamster ovary (CHO) cells have been investigated after reacting cells at 4 degrees C with the membrane-impermeant reagent, trinitrobenzenesulfonate (TNBS). Immunoprecipitation and subsequent two-dimensional, sodium-dodecyl sulfate, polyacrylamide gel electrophoresis (SDS-PAGE...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(86)90534-3

    authors： Raub TJ,Denny JB,Roberts RM

    更新日期：1986-07-01 00:00:00

  • Intranuclear distribution of SV40 large T-antigen and transformation-related protein p53 in abortively infected cells.

    abstract：:The intranuclear localization of SV40 T-antigen (T-Ag) and the cellular protein p53 was studied in SV40 abortively infected baby mouse kidney cells using two complementary methods of ultrastructural immunocytochemistry in combination with preferential staining of nuclear RNP components and electron microscope autoradi...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(88)90026-2

    authors： Puvion E,Duthu A,Harper F,Ehrhart JC,Viron A,May P

    更新日期：1988-07-01 00:00:00

  • Guidance and activation of murine macrophages by nanometric scale topography.

    abstract：:We studied the guidance and activation of macrophages from the P388D1 cell line and rat peritoneum by topographic features on a nanometric scale. Cells were plated on plain fused silica substrata or substrata with microfabricated grooves and steps, 30-282 nm deep. The contact of cells with the patterned surface activa...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1996.0098

    authors： Wójciak-Stothard B,Curtis A,Monaghan W,MacDonald K,Wilkinson C

    更新日期：1996-03-15 00:00:00

  • CHO cell aggregation induced by fibronectin-coated beads. Differences between wild-type and adhesion-variant cells.

    abstract：:ADvF11 (F11), a Chinese Hamster Ovary (CHO) cell variant, is defective in its ability to adhere to fibronectin (Fn)-coated substrata but will adhere to substrata coated with poly-L-lysine, conA or extracellular matrix (ECM) [1]. We have observed that both F11 and CHO wild-type (WT) cells were able to bind 3H-Fn beads ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(84)90236-2

    authors： Cheung E,Juliano RL

    更新日期：1984-05-01 00:00:00

  • Conjugation in the ciliate Euplotes octocarinatus: comparison of ciliary and cell body-associated glycoconjugates of non-mating-competent, mating-competent, and conjugating cells.

    abstract：:Pair formation in the hypotrichous ciliate Euplotes octocarinatus is a poorly understood phenomenon. In order to obtain information about the molecules involved in this process, we compared ciliary and cell body-associated glycoconjugates of non-mating-competent, mating-competent, and conjugating cells. Detection of g...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1995.1114

    authors： Plümper E,Freiburg M,Heckmann K

    更新日期：1995-04-01 00:00:00

  • Characterization of lamina-bound chromatin in the nuclear shell isolated from HeLa cells.

    abstract：:The structure and the polypeptide composition of the nuclear shell isolated from interphase HeLa cells have been investigated and compared to those of the intranuclear material. The isolated nuclear shell contains chromatin superstructures (28-32 nm thick fibres) made of tightly packed nucleosomes that resist low ioni...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(85)90557-9

    authors： Bouvier D,Hubert J,Seve AP,Bouteille M

    更新日期：1985-02-01 00:00:00

  • Different strategies of X-inactivation in germinal and somatic cells: histone H4 underacetylation does not mark the inactive X chromosome in the mouse male germline.

    abstract：:It has previously been shown by immunocytochemistry that the inactive X chromosome (Xi) in somatic cells of human and mouse females is marked by underacetylation of histone H4. It has been suggested that this may be important for transcriptional silencing of genes on Xi. We have now investigated X-inactivation in meio...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1996.3394

    authors： Armstrong SJ,Hultén MA,Keohane AM,Turner BM

    更新日期：1997-02-01 00:00:00

  • The actin cytoskeleton-associated protein zyxin acts as a tumor suppressor in Ewing tumor cells.

    abstract：:Changes in cell architecture, essentially linked to profound cytoskeleton rearrangements, are common features accompanying cell transformation. Supporting the involvement of the microfilament network in tumor cell behavior, several actin-binding proteins, including zyxin, a potential regulator of actin polymerization,...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2004.10.035

    authors： Amsellem V,Kryszke MH,Hervy M,Subra F,Athman R,Leh H,Brachet-Ducos C,Auclair C

    更新日期：2005-04-01 00:00:00

  • Polyphosphate induces matrix metalloproteinase-3-mediated proliferation of odontoblast-like cells derived from induced pluripotent stem cells.

    abstract：:Inorganic polyphosphate [Poly(P)] may represent a physiological source of phosphate and has the ability to induce bone differentiation in osteoblasts. We previously reported that cytokine-induced matrix metalloproteinase (MMP)-3 accelerates the proliferation of purified odontoblast-like cells. In this study, MMP-3 sma...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2015.01.007

    authors： Ozeki N,Hase N,Yamaguchi H,Hiyama T,Kawai R,Kondo A,Nakata K,Mogi M

    更新日期：2015-05-01 00:00:00

  • Nanosecond pulsed electric field (nsPEF) enhance cytotoxicity of cisplatin to hepatocellular cells by microdomain disruption on plasma membrane.

    abstract：:Previous studies showed nanosecond pulsed electric field (nsPEF) can ablate solid tumors including hepatocellular carcinoma (HCC) but its effect on cell membrane is not fully understood. We hypothesized nsPEF disrupt the microdomains on outer-cellular membrane with direct mechanical force and as a result the plasma me...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2016.06.018

    authors： Yin S,Chen X,Xie H,Zhou L,Guo D,Xu Y,Wu L,Zheng S

    更新日期：2016-08-15 00:00:00

  • Effects of simian virus 40 T-antigens on normal human mammary epithelial cells reveal evidence for spontaneous alterations in addition to loss of p16(INK4a) expression.

    abstract：:Under standard culture conditions, normal human mammary epithelial cells (HMECs) divide a limited number of times before proliferation ceases in a growth-arrested state referred to as selection. Cells that have undergone spontaneous loss of p16(INK4a) expression due to hypermethylation of the p16(INK4a) CpG island eme...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2001.5178

    authors： Huschtscha LI,Neumann AA,Noble JR,Reddel RR

    更新日期：2001-04-15 00:00:00

  • Epigenetic specification of centromeres by CENP-A.

    abstract：:Centromeres are the chromosomal loci that direct the formation of the kinetochores. These macromolecular assemblies mediate the interaction between chromosomes and spindle microtubules and thereby power chromosome movement during cell division. They are also the sites of extensive regulation of the chromosome segregat...

    journal_title：Experimental cell research

    pub_type： 杂志文章,评审

    doi：10.1016/j.yexcr.2009.07.023

    authors： Bernad R,Sánchez P,Losada A

    更新日期：2009-11-15 00:00:00

  • Induction of tyrosinase in human melanoma cells by L-tyrosine phosphate and cytochalasin D.

    abstract：:Pigmentation of RVH 421 human melanoma cells is induced when cell division is inhibited by cytochalasin D or L-tyrosine phosphate. Increased pigmentation correlates with increased tyrosinase activity when this is monitored over a time-course. Parallel measurements show that the amount of tyrosinase mRNA correlates wit...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(92)90431-7

    authors： Winder AJ,Harris H

    更新日期：1992-04-01 00:00:00

  • Interaction of methylmercury with microtubules in cultured cells and in vitro.

    abstract：:The effects of methylmercury (MeHg) on cytoplasmic microtubules in cultured fibroblasts and on the in vitro polymerization of microtubules were examined. MeHg caused disruption of cellular microtubules in a concentration- and time-dependent manner. Addition of the metal-chelating agent, dimercaptosuccinic acid (DMSA),...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(83)90331-2

    authors： Sager PR,Doherty RA,Olmsted JB

    更新日期：1983-06-01 00:00:00

  • Inhibition of the mitochondrial calcium uniporter prevents IL-13 and allergen-mediated airway epithelial apoptosis and loss of barrier function.

    abstract：:Mitochondria are increasingly recognized as key mediators of acute cellular stress responses in asthma. However, the distinct roles of regulators of mitochondrial physiology on allergic asthma phenotypes are currently unknown. The mitochondrial Ca2+ uniporter (MCU) resides in the inner mitochondrial membrane and contr...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2017.12.003

    authors： Sebag SC,Koval OM,Paschke JD,Winters CJ,Comellas AP,Grumbach IM

    更新日期：2018-01-15 00:00:00

  • Critical roles of CTP synthase N-terminal in cytoophidium assembly.

    abstract：:Several metabolic enzymes assemble into distinct intracellular structures in prokaryotes and eukaryotes suggesting an important functional role in cell physiology. The CTP-generating enzyme CTP synthase forms long filamentous structures termed cytoophidia in bacteria, yeast, fruit flies and human cells independent of ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2017.03.042

    authors： Huang Y,Wang JJ,Ghosh S,Liu JL

    更新日期：2017-05-15 00:00:00