Nanosecond pulsed electric field (nsPEF) enhance cytotoxicity of cisplatin to hepatocellular cells by microdomain disruption on plasma membrane.


:Previous studies showed nanosecond pulsed electric field (nsPEF) can ablate solid tumors including hepatocellular carcinoma (HCC) but its effect on cell membrane is not fully understood. We hypothesized nsPEF disrupt the microdomains on outer-cellular membrane with direct mechanical force and as a result the plasma membrane permeability increases to facilitate the small molecule intake. Three HCC cells were pulsed one pulse per minute, an interval longer than nanopore resealing time. The cationized ferritin was used to mark up the electronegative microdomains, propidium iodide (PI) for membrane permeabilization, energy dispersive X-ray spectroscopy (EDS) for the negative cell surface charge and cisplatin for inner-cellular cytotoxicity. We demonstrated that the ferritin marked-microdomain and negative cell surface charge were disrupted by nsPEF caused-mechanical force. The cell uptake of propidium and cytotoxicity of DNA-targeted cisplatin increased with a dose effect. Cisplatin gains its maximum inner-cellular cytotoxicity when combining with nsPEF stimulation. We conclude that nsPEF disrupt the microdomains on the outer cellular membrane directly and increase the membrane permeabilization for PI and cisplatin. The microdomain disruption and membrane infiltration changes are caused by the mechanical force from the changes of negative cell surface charge.


Exp Cell Res


Yin S,Chen X,Xie H,Zhou L,Guo D,Xu Y,Wu L,Zheng S




Has Abstract


2016-08-15 00:00:00














  • Disruption of cytoplasmic microtubules by ultraviolet radiation.

    abstract::Ultraviolet (UV) irradiation of cultured human skin fibroblasts causes the disassembly of their microtubules. Using indirect immunofluorescence microscopy, we have now investigated whether damage to the microtubule precursor pool may contribute to the disruption of microtubules. Exposure to polychromatic UV radiation ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Zamansky GB,Perrino BA,Chou IN

    更新日期:1991-07-01 00:00:00

  • Tenogenic differentiation of mesenchymal stem cells and noncoding RNA: From bench to bedside.

    abstract::Tendon is a critical unit of musculoskeletal system that connects muscle to bone to control bone movement. More population participate in physical activities, tendon injuries, such as acute tendon rupture and tendinopathy due to overuse, are common causing unbearable pain and disability. However, the process of tendon...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Lu YF,Chan KM,Li G,Zhang JF

    更新日期:2016-02-15 00:00:00

  • Tks5 recruits AFAP-110, p190RhoGAP, and cortactin for podosome formation.

    abstract::Podosome formation in vascular smooth muscle cells is characterized by the recruitment of AFAP-110, p190RhoGAP, and cortactin, which have specific roles in Src activation, local down-regulation of RhoA activity, and actin polymerization, respectively. However, the molecular mechanism that underlies their specific recr...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Crimaldi L,Courtneidge SA,Gimona M

    更新日期:2009-09-10 00:00:00

  • Immunofluorescent localization of histone H10 in the nuclei of proliferating and differentiating Friend cells.

    abstract::By using affinity-purified antibodies to H10 and to H1AB the localization of these histones was studied by indirect immunofluorescence in the nuclei of proliferating (EAT and uninduced Friend cells) and of differentiating (induced Friend cells) cell populations. While with H1AB antibodies a bright fluorescence all ove...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Banchev T,Srebreva L,Zlatanova J,Tsanev R

    更新日期:1988-07-01 00:00:00

  • Heterotrimeric G-proteins are implicated in the regulation of apoptosis in pancreatic beta-cells.

    abstract::Recent studies have provided evidence that apoptosis of pancreatic beta-cells is important in the early etiology of both type I and type II diabetes mellitus. The mechanisms responsible for induction of apoptosis are unknown, but we present evidence that the signal transduction pathway controlling the process in pancr...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Loweth AC,Williams GT,Scarpello JH,Morgan NG

    更新日期:1996-11-25 00:00:00

  • miR-34a expands myeloid-derived suppressor cells via apoptosis inhibition.

    abstract::Myeloid-derived suppressor cells (MDSCs) are a heterogeneous population and show significant expansion under pathological conditions. microRNA plays important roles in many biological processes, whether microRNAs have a function in the expansion of MDSCs is still not very clear. In this study, miR-34a overexpression c...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Huang A,Zhang H,Chen S,Xia F,Yang Y,Dong F,Sun D,Xiong S,Zhang J

    更新日期:2014-08-15 00:00:00

  • Intracellular protein delivery activity of peptides derived from insulin-like growth factor binding proteins 3 and 5.

    abstract::Insulin-like growth factor binding proteins (IGFBPs) have various IGF-independent cellular activities, including receptor-independent cellular uptake followed by transcriptional regulation, although mechanisms of cellular entry remain unclear. Herein, we focused on their receptor-independent cellular entry mechanism i...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Goda N,Tenno T,Inomata K,Shirakawa M,Tanaka T,Hiroaki H

    更新日期:2008-08-01 00:00:00

  • Microparticles released by human neutrophils adhere to erythrocytes in the presence of complement.

    abstract::The release of cell surface-derived microparticles, or ectosomes, has now been described for many different cell types. In various diseases characterized by systemic inflammation, the numbers of ectosomes released from specific cell-types are found increased manifold in the circulation. Their pro-inflammatory and pro-...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Gasser O,Schifferli JA

    更新日期:2005-07-15 00:00:00

  • Basic FGF and TGF-beta differentially modulate integrin expression of human microvascular endothelial cells.

    abstract::Basic fibroblast growth factor (bFGF) and transforming growth factor-beta (TGF-beta) are known to alter the migratory and proliferative capacity of endothelial cells in vitro and to stimulate angiogenesis in vivo. One mechanism by which these cytokines induce their effects may be through the regulation of integrin adh...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Enenstein J,Waleh NS,Kramer RH

    更新日期:1992-12-01 00:00:00

  • Small interfering RNA targeting ILK inhibits metastasis in human tongue cancer cells through repression of epithelial-to-mesenchymal transition.

    abstract::Integrin-linked kinase (ILK) is a multifunctional serine/threonine kinase. Accumulating evidences suggest that ILK are involved in cell-matrix interactions, cell proliferation, invasion, migration, angiogenesis and Epithelial-mesenchymal transition (EMT). However, the underlying mechanisms remain largely unknown. EMT ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Xing Y,Qi J,Deng S,Wang C,Zhang L,Chen J

    更新日期:2013-08-01 00:00:00

  • Regulation of pyruvate kinase expression and growth in mastocytoma cells. I. Initial observations.

    abstract::The specific activities of pyruvate kinase and phosphofructokinase but not lactate dehydrogenase increase as P-815 mastocytoma cells approach the stationary phase. During this growth period, the rates of uptake of labelled precursors into DNA, RNA and total protein decreases. On the other hand, the pyruvate kinase pro...

    journal_title:Experimental cell research

    pub_type: 杂志文章


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  • Eps8 is recruited to lysosomes and subjected to chaperone-mediated autophagy in cancer cells.

    abstract::Eps8 controls actin dynamics directly through its barbed end capping and actin-bundling activity, and indirectly by regulating Rac-activation when engaged into a trimeric complex with Eps8-Abi1-Sos1. Recently, Eps8 has been associated with promotion of various solid malignancies, but neither its mechanisms of action n...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Welsch T,Younsi A,Disanza A,Rodriguez JA,Cuervo AM,Scita G,Schmidt J

    更新日期:2010-07-15 00:00:00

  • Fusion-mediated implantation of band 3 into living cells. A new system to study degradation of membrane proteins.

    abstract::Sendai virus (SV) glycoproteins were co-reconstituted with the erythrocyte membrane polypeptide designated as band 3 (B3). Fusion of the hybrid vesicles, SV-B3, with L, HeLa and Friend erythroleukemic (FEL) cells led to fusion-mediated implantation of the erythrocyte band 3 into the plasma membranes of the above cells...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Beigel M,Loyter A

    更新日期:1983-10-01 00:00:00

  • Dysregulation of Ack1 inhibits down-regulation of the EGF receptor.

    abstract::The protein tyrosine kinase Ack1 has been linked to cancer when over-expressed. Ack1 has also been suggested to function in clathrin-mediated endocytosis and in down-regulation of the epidermal growth factor (EGF) receptor (EGFR). We have studied the intracellular localization of over-expressed Ack1 and found that Ack...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Grøvdal LM,Johannessen LE,Rødland MS,Madshus IH,Stang E

    更新日期:2008-04-01 00:00:00

  • cxcl12 gene engineered endothelial progenitor cells further improve the functions of oligodendrocyte precursor cells.

    abstract::Oligodendrocyte precursor cells (OPCs) are needed for white matter repair after various brain injury. Means that promote OPC functions could benefit white matter recovery after injury. Chemokine CXCL12 and endothelial progenitor cells (EPCs) both have been shown to promote remyelination. We hypothesize that the benefi...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Yuan F,Chang S,Luo L,Li Y,Wang L,Song Y,Qu M,Zhang Z,Yang GY,Wang Y

    更新日期:2018-06-15 00:00:00

  • The early manifestation, tumor-specific occurrence and prognostic significance of TGFBR2 aberrant splicing in oral carcinoma.

    abstract::Alternative splicing is an important mechanism that can disrupt cell cycle control resulting in tumorigenesis. Although many alterations of Transforming Growth Factor Beta (TGFβ) signaling are reported in cancers, the role of splice aberrations in destabilizing this signaling is the least understood mechanism. In this...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Sivadas VP,Gulati S,Varghese BT,Balan A,Kannan S

    更新日期:2014-09-10 00:00:00

  • Abrogation of TGFbeta signaling induces apoptosis through the modulation of MAP kinase pathways in breast cancer cells.

    abstract::Transforming growth factor beta (TGFbeta) can modulate the activity of various MAP kinases. However, how this pathway may mediate TGFbeta-induced malignant phenotypes remains elusive. We investigated the role of autocrine TGFbeta signaling through MAP kinases in the regulation of cell survival in breast carcinoma MCF-...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Lei X,Yang J,Nichols RW,Sun LZ

    更新日期:2007-05-01 00:00:00

  • Co-localization of the retinoblastoma protein and the Epstein-Barr virus-encoded nuclear antigen EBNA-5.

    abstract::A monoclonal antibody (aRB1C1) raised against an Rb fusion protein detects a limited number (4-10) of relatively large intranuclear foci in an EBV-immortalized cord blood cell line (IB4). These domains also bind an anti-EBNA-5 monoclonal antibody. The Rb antibody reactive sites also co-localize with the SV40 T antigen...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Jiang WQ,Szekely L,Wendel-Hansen V,Ringertz N,Klein G,Rosén A

    更新日期:1991-12-01 00:00:00

  • TIEG1-null tenocytes display age-dependent differences in their gene expression, adhesion, spreading and proliferation properties.

    abstract::The remodeling of extracellular matrix is a crucial mechanism in tendon development and the proliferation of fibroblasts is a key factor in this process. The purpose of this study was to further elucidate the role of TIEG1 in mediating important tenocyte properties throughout the aging process. Wildtype and TIEG1 knoc...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Haddad O,Gumez L,Hawse JR,Subramaniam M,Spelsberg TC,Bensamoun SF

    更新日期:2011-07-15 00:00:00

  • S632A3, a new glutarimide antibiotic, suppresses lipopolysaccharide-induced pro-inflammatory responses via inhibiting the activation of glycogen synthase kinase 3β.

    abstract::Inflammatory mediators including inducible nitric oxide (iNOS), cyclooxygenase-2 (COX-2), tumor necrosis factor-α (TNF-α) and Interleukin-6 (IL-6) contribute to the course of a variety of inflammatory diseases. S632A3 is a new member of the glutarimide antibiotics isolated from a cultured broth of Streptomyces hygrosc...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Deng H,Zhang N,Wang Y,Chen J,Shen J,Wang Z,Xu R,Zhang J,Song D,Li D

    更新日期:2012-12-10 00:00:00

  • Skeletal myocyte hypertrophy requires mTOR kinase activity and S6K1.

    abstract::The protein kinase mammalian target of rapamycin (mTOR) is a central regulator of cell proliferation and growth, with the ribosomal subunit S6 kinase 1 (S6K1) as one of the key downstream signaling effectors. A critical role of mTOR signaling in skeletal muscle differentiation has been identified recently, and an unus...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Park IH,Erbay E,Nuzzi P,Chen J

    更新日期:2005-09-10 00:00:00

  • Morphological and functional effects of extracellular matrix on pancreatic islet cell cultures.

    abstract::Extracellular matrix (ECM) has been reported to enhance epithelial cell attachment and proliferation as well as to induce differentiation in vitro. In an attempt to determine the benefits of culturing pancreatic islet cells on ECM, we studied the morphological and functional patterns of rat islet cells and an insulin-...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Thivolet CH,Chatelain P,Nicoloso H,Durand A,Bertrand J

    更新日期:1985-08-01 00:00:00

  • Epigenetic and HIF-1 regulation of stanniocalcin-2 expression in human cancer cells.

    abstract::Mammalian stanniocalcin-2 (STC2) is a secreted glycoprotein hormone with a putative role in unfolded protein response and apoptosis. Here we reported that STC2 expression was sporadically abrogated in human cancer cells by transcriptional silencing associated with CpG island promoter hypermethylation. Direct sequencin...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Law AY,Lai KP,Ip CK,Wong AS,Wagner GF,Wong CK

    更新日期:2008-05-01 00:00:00

  • Melanoma cells produce multiple laminin isoforms and strongly migrate on α5 laminin(s) via several integrin receptors.

    abstract::Melanoma cells express and interact with laminins (LMs) and other basement membrane components during invasion and metastasis. In the present study we have investigated the production and migration-promoting activity of laminin isoforms in melanoma. Immunohistochemistry of melanoma specimens and immunoprecipitation/we...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Oikawa Y,Hansson J,Sasaki T,Rousselle P,Domogatskaya A,Rodin S,Tryggvason K,Patarroyo M

    更新日期:2011-05-01 00:00:00

  • Effects of antiandrogens on chromatin remodeling and transcription of the integrated mouse mammary tumor virus promoter.

    abstract::Inhibition of the ligand-activated androgen receptor (AR) by antiandrogens plays an important role in the treatment of various hyperandrogenic disorders including prostate cancer. However, the molecular mechanisms of antiandrogen activity in vivo remain unclear. In this study we analyzed the effects of cyproterone ace...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: List HJ,Smith CL,Martinez E,Harris VK,Danielsen M,Riegel AT

    更新日期:2000-10-10 00:00:00

  • The impact of maternal serum on development of enolase activity in fetal rat brain cell culture.

    abstract::The effect of gestational age on serum-mediated changes in enolase activity was tested in a fetal rat brain cell culture. After 6 days exposure to graded concentrations (10 and 20%) of nonpregnant female rat sera, enolase activity in brain cell cultures increased from 2.83 +/- 0.03 to 3.74 +/- 0.19 mumol/min/mg protei...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Shambaugh GE 3rd,Clough D,Munari L,Koehler RR,Radosevich JA

    更新日期:1990-02-01 00:00:00

  • Nucleocytoplasmic shuttling of STK16 (PKL12), a Golgi-resident serine/threonine kinase involved in VEGF expression regulation.

    abstract::PKL12/STK16 protein is the first identified mammalian member of a ser/thr kinase subfamily that is conserved across several kingdoms, with a broad expression pattern in murine tissues and cell types. Endogenous STK16 subcellular localization was evaluated by indirect immunofluorescence in NIH/3T3 and NRK cells, demons...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Guinea B,Ligos JM,Laín de Lera T,Martín-Caballero J,Flores J,Gonzalez de la Peña M,García-Castro J,Bernad A

    更新日期:2006-01-15 00:00:00

  • PLCgamma participates in insulin stimulation of glucose uptake through activation of PKCzeta in brown adipocytes.

    abstract::Based on recent studies showing that PLCgamma associates to insulin receptor, we investigated its role in insulin stimulation of glucose transport in brown adipocytes. Insulin stimulation induced rapid PLCgamma association to phosphorylated insulin receptor, and activation of PLCgamma, as assessed by the mobilization ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Lorenzo M,Teruel T,Hernandez R,Kayali AG,Webster NJ

    更新日期:2002-08-15 00:00:00

  • FR901228, a potent antitumor antibiotic, is a novel histone deacetylase inhibitor.

    abstract::Screening for microbial metabolites that induce transcriptional activation of the SV40 promoter resulted in the identification of two known compounds, FR901228 and trichostatin A (TSA). FR901228 is a potent antitumor drug that is currently under clinical investigation. TSA is a specific inhibitor of histone deacetylas...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Nakajima H,Kim YB,Terano H,Yoshida M,Horinouchi S

    更新日期:1998-05-25 00:00:00

  • Mesenchymal stromal cells revert multiple myeloma cells to less differentiated phenotype by the combined activities of adhesive interactions and interleukin-6.

    abstract::Multiple myeloma is characterized by the malignant growth of immunoglobulin producing plasma cells, predominantly in the bone marrow. The effects of primary human mesenchymal stromal cells on the differentiation phenotype of multiple myeloma cells were studied by co-culture experiments. The incubation of multiple myel...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Dezorella N,Pevsner-Fischer M,Deutsch V,Kay S,Baron S,Stern R,Tavor S,Nagler A,Naparstek E,Zipori D,Katz BZ

    更新日期:2009-07-01 00:00:00