Abstract:
:The effect of transferrin was tested on osteoblastic cells (clone MC3T3-E1) cultured in serum-free medium containing 1% bovine serum albumin (BSA). Transferrin (Tf) stimulated increases of protein content and protein synthesis, but not of DNA content and cell number, in the cells. This protein also increased alkaline phosphatase activity and collagen synthesis in combination with 1% BSA. Actinomycin D and cycloheximide inhibited alkaline phosphatase activity induced by Tf, suggesting that Tf may enhance de novo synthesis of the enzyme. These results indicate that Tf may be involved in differentiation of osteoblastic cells, but not in their proliferation, in vitro.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Tsunoi M,Hakeda Y,Kurihara N,Maeda N,Utsumi N,Kumegawa Mdoi
10.1016/0014-4827(84)90465-8subject
Has Abstractpub_date
1984-07-01 00:00:00pages
240-4issue
1eissn
0014-4827issn
1090-2422pii
0014-4827(84)90465-8journal_volume
153pub_type
杂志文章abstract::The glycolytic enzyme aldolase is concentrated in a domain around stress fibers in living Swiss 3T3 cells, but the mechanism by which aldolase is localized has not been revealed. We have recently identified a molecular binding site for F-actin on aldolase, and we hypothesized that this specific binding interaction, ra...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1997.3811
更新日期:1997-12-15 00:00:00
abstract::Transcription profiling of early embryos emphasizes that differential gene expression is a fundamental control mechanism of development. Precise regulatory mechanisms operate on a background of epigenetic changes in chromatin composition, modification, and architecture that are integral for the development of a plurip...
journal_title:Experimental cell research
pub_type: 杂志文章,评审
doi:10.1016/j.yexcr.2005.06.023
更新日期:2005-10-01 00:00:00
abstract::A popular criterion of cell-cell communication in tissue cultures is dye coupling: the ability of the injected fluorescent dye of low molecular weight to be transferred from one cell to another. We report about a new factor which induces cell-to-cell dye coupling in previously uncoupled epithelial sheets. Paradoxicall...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(88)90204-2
更新日期:1988-04-01 00:00:00
abstract::We have examined the effects of diamide, an oxidizer of glutathione, on the progress of HeLa cells through the cell cycle. At concentrations which do not significantly alter generation time, anaphase or cytokinesis, diamide causes a two-fold increase in the duration of metaphase. At 3 X 10(-8) M, ouabain also prolongs...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(85)90449-5
更新日期:1985-05-01 00:00:00
abstract::Quinolinic acid (QUIN) is a glutamate agonist which markedly enhances the vulnerability of neural cells to excitotoxicity. QUIN is produced from the amino acid tryptophan through the kynurenine pathway (KP). Dysregulation of this pathway is associated with neurodegenerative conditions. In this study we treated striata...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2014.02.024
更新日期:2014-04-01 00:00:00
abstract::Mesenchymal stem cells are universally regarded across many fields of medicine, as one of the most promising cell types for use in cell-based therapies. Although not yet fully understood, the therapeutic effects of these cells are largely attributed to the trophic actions of growth factors and cytokines present in the...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2014.12.019
更新日期:2015-04-10 00:00:00
abstract::Estrogen receptor-containing cells were identified in uteri of mature and senescent rats by means of quantitative autoradiography. Cell numbers and density were assessed in the various uterine layers. It was determined that despite decreases in receptor concentrations in endometrium, endometrial stroma, myometrium, an...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(89)90227-9
更新日期:1989-01-01 00:00:00
abstract::Plasmid 6.4 kbp DNA, 14 kbp DNA, lambda phage particles, all of which contained herpes simplex virus type 1 (HSV-1) thymidine kinase (TK) gene, or IgM molecules, were mixed with erythrocyte membranes and treated with neutral detergent. The transparent mixture was diluted with phosphate-buffered saline (PBS), followed ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/s0014-4827(85)80014-8
更新日期:1985-08-01 00:00:00
abstract::Apoptosis is characterized by typical morphological changes and most frequently fragmentation of DNA into oligonucleosome-size fragments. In order to investigate whether an alteration in the mechanisms involved in the process of apoptosis could contribute to cellular resistance, induction of apoptosis was studied in a...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1995.1148
更新日期:1995-05-01 00:00:00
abstract::Müller cells are the predominant retinal glial cells. One of the key roles of Müller cells is in the uptake of the neurotransmitter glutamate and in its conversion to glutamine. Müller cell dysfunction due to oxidative stress elicited by high glutamate concentrations can lead to toxicity, which promote the pathogenesi...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2019.111509
更新日期:2019-10-01 00:00:00
abstract::Abnormal aspartyl residue formation such as L-isoaspartates occurs frequently during aging in long-lived proteins, resulting in the alteration of their structures and biological functions. In this study, we investigated the alteration of aspartyl residues in extracellular matrix (ECM) proteins, type-I collagen and fib...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2003.10.003
更新日期:2004-02-01 00:00:00
abstract::The squamous non-keratinizing epithelium of the human upper digestive tract was analyzed for keratin-like cytoskeletal proteins (cytokeratins) by both high resolution one- and two-dimensional gel electrophoresis. The Triton/high salt-insoluble portion of pure epithelial homogenates contains a number of SDS- and urea-e...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(86)90430-1
更新日期:1986-01-01 00:00:00
abstract::CEACAM1-4S (carcinoembryonic antigen-related cell adhesion molecule 1) is a type I membrane protein with a short (12-amino acid) cytoplasmic tail. Wild type CEACAM1-4S-transfected MCF7 cells form glands with lumena when grown in 3D culture, while null mutations of two putative phosphorylation sites (T457A and S459A) i...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2009.11.001
更新日期:2010-02-15 00:00:00
abstract::The intracellular distribution of the glucocorticosteroid and progesterone receptors (GR and PR, respectively) was studied immunohistochemically. In control adrenalectomized (Adx) rat liver, immunostaining of paraffin sections revealed GR in cell nuclei, with a wide range of intensity between individuals. Following de...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(89)90106-7
更新日期:1989-04-01 00:00:00
abstract::Mitotic PtK1 cells were treated at furrow initiation with microtubule poisons to determine the role of microtubules in the regulation of terminal cytokinetic events. The administration of anti-microtubule agents in late anaphase accelerated the rate of cytokinesis by approx. 60% as measured by changes in furrow diamet...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(83)90413-5
更新日期:1983-04-01 00:00:00
abstract::The differential activation of cyclin and cyclin-dependent kinase genes by the adenovirus E1A gene product (E1A) or serum factors was studied with a rat 3Y1 derivative cell line, g12-21, in which the E1A12S cDNA can be expressed in response to dexamethasone (dex). The induction of DNA synthesis in quiescent g12-21 cel...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1993.1262
更新日期:1993-10-01 00:00:00
abstract::VEGF is a major inducer of angiogenesis. However, the homing role of VEGF for cardiac stem cells (CSCs) is unclear. In in vitro experiments, CSCs were isolated from the rat hearts, and a cellular migration assay was performed using a 24-well transwell system. VEGF induced CSC migration in a concentration-dependent man...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2009.09.026
更新日期:2009-12-10 00:00:00
abstract::Previous studies have shown that ascorbic acid increases both the total surface acetylcholine receptor (AChR) expression and the mRNA for the alpha-subunit of this receptor in myotubes of cloned L5 muscle cultures. Since ascorbic acid increases collagen synthesis in fibroblasts, we studied the effect of ascorbic acid ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1993.1287
更新日期:1993-11-01 00:00:00
abstract::Insulin-like growth factor-1 (IGF-1) and its receptor (IGF-1R) are required for cell proliferation in vitro, raising the possibility that an autocrine IGF-1-IGF-1R system may be present in vivo and become activated in the viable ventricular myocytes shortly after infarction. Therefore, following the in vivo documentat...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1994.1224
更新日期:1994-08-01 00:00:00
abstract::Pevonedistat is a potent, selective, first-in-class NEDD8 activating enzyme inhibitor. It is now under multiple clinical trials that investigate its anticancer effect against solid tumors and leukemia. ATP-binding cassette (ABC) transporters are membrane proteins that are involved in mediating multidrug resistance (MD...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2020.111858
更新日期:2020-03-15 00:00:00
abstract::Vigilin, a protein with a continuous series of 14 KH motifs, forms part of a multiprotein complex containing tRNA. Several lines of evidence have suggested that vigilin expression is enhanced in those cells which were actively engaged in protein synthesis. Accordingly, we show here by immunoelectronmicroscopy a close ...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1997.3891
更新日期:1998-02-25 00:00:00
abstract::Gp38k is a 383-amino-acid secreted glycoprotein expressed by cultured vascular smooth muscle cells during the time of transition from a proliferating monolayer culture to a nonproliferating multilayered (differentiated) culture. Expression continues as the cell culture forms multicellular nodules. Because this transit...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1999.4511
更新日期:1999-07-10 00:00:00
abstract::The distribution and organisation of F-actin during the cell cycle of meristematic root-tip cells of Allium was investigated using a rhodamine-labelled phalloidin to stain F-actin in isolated cell preparations. Such preparations could, in addition, be stained for tubulin by immunofluorescence, enabling a comparison be...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(85)90277-0
更新日期:1985-01-01 00:00:00
abstract::Cytostatic factor (CSF), found in the cytoplasm of unfertilized eggs of amphibians, causes metaphase arrest when microinjected into cleaving blastomeres. Although CSF from Rana pipiens eggs has been extracted and characterized, little is known about CSF extracted from eggs of other species. We investigated the conditi...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(90)90211-r
更新日期:1990-01-01 00:00:00
abstract::Retinol binding protein (RBP) is the plasma transport protein of retinol. Mobilization of RBP from the liver stores is stimulated by retinol. During vitamin A deficiency, RBP secretion is specifically inhibited while its rate of biosynthesis is unaffected. As a consequence, RBP, as apoprotein, accumulates inside the e...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(92)90197-g
更新日期:1992-06-01 00:00:00
abstract::Strategies for correct diagnosis, treatment evaluation and recurrence prediction are important for the prognosis and mortality rates among cancer patients. In spite of major improvements in clinical management, gastrointestinal stromal tumors (GISTs) can still be deadly due to metastasis and recurrences, which confirm...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2015.05.004
更新日期:2015-08-01 00:00:00
abstract::We previously demonstrated that the Tg737 gene plays a critical role in the carcinogenesis of hepatocellular carcinoma (HCC). However, few systematic investigations have focused on the biological function of Tg737 in the invasion and migration of liver cancer stem cells (LCSCs) and on its clinical significance. In thi...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2017.06.021
更新日期:2017-09-15 00:00:00
abstract::Procollagen I is a trimer consisting of two proalpha1(I) chains and one proalpha 2(I) chain. In certain cases of mild osteogenesis imperfecta, abnormal proalpha1(I) chains are degraded very soon after synthesis. As a consequence, the cells produce excess proalpha2(I) chains, which cannot form trimers and are not secre...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/j.yexcr.2004.01.029
更新日期:2004-06-10 00:00:00
abstract::Cell migration plays a major role during wound healing and is tightly controlled by a variety of growth factors and extracellular matrix proteins. The experiments reported here have been designed to study whether defined beta 1 integrins are involved in the platelet-derived growth-factor-AB (PDGF-AB)-modulated migrato...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1006/excr.1995.1288
更新日期:1995-09-01 00:00:00
abstract::Liver cells isolated from the adult rat livers under mild conditions were preincubated for 1 day with Williams medium E (WE) containing serum, dexamethasone and insulin, and then the cells (monolayered) were incubated for 2-3 days with WE (1 ml) containing only insulin to measure DNA synthesis and/or mitosis. DNA synt...
journal_title:Experimental cell research
pub_type: 杂志文章
doi:10.1016/0014-4827(84)90196-4
更新日期:1984-12-01 00:00:00