Abstract：
:The distribution and organisation of F-actin during the cell cycle of meristematic root-tip cells of Allium was investigated using a rhodamine-labelled phalloidin to stain F-actin in isolated cell preparations. Such preparations could, in addition, be stained for tubulin by immunofluorescence, enabling a comparison between F-actin and microtubule distributions in the same cell. In interphase, an extensive array of actin-filament bundles was present in the cytoplasm of elongating cells, the bundles generally following the long axis of the cell and passing in close proximity to the nucleus. In contrast, the interphase microtubule array occupied the cortex of the cell and was oriented at right angles to the actin bundles. In smaller, isodiametric cells, microfilament arrays were present but less well developed. During cell division, phalloidin-specific staining was seen in the cytokinetic phragmoplast, and co-distributed with microtubules at all stages of cell plate formation; however, neither the pre-prophase band nor the mitotic spindle were stained with phalloidin. Co-distribution of F-actin and microtubules only occurs, therefore, at cytokinesis. The relationship between microfilaments and microtubules is discussed, together with the possible role of actin in the phragmoplast.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Clayton L,Lloyd CWdoi
10.1016/0014-4827(85)90277-0subject
Has Abstractpub_date
1985-01-01 00:00:00pages
231-8issue
1eissn
0014-4827issn
1090-2422pii
0014-4827(85)90277-0journal_volume
156pub_type
杂志文章abstract：:Mammalian stanniocalcin-2 (STC2) is a secreted glycoprotein hormone with a putative role in unfolded protein response and apoptosis. Here we reported that STC2 expression was sporadically abrogated in human cancer cells by transcriptional silencing associated with CpG island promoter hypermethylation. Direct sequencin...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2008.03.001
更新日期：2008-05-01 00:00:00
abstract：:In a previous study, we found that a specific inhibitor of cellular arginine-specific mono(ADP-ribosyl) transferase, meta-iodobenzylguanidine (MIBG), reversibly inhibited both proliferation and differentiation of cultured embryonic chick primary muscle myoblasts. In addition, we observed that arginine-specific ADP-rib...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1996.0213
更新日期：1996-07-10 00:00:00
abstract：:We have recently demonstrated that thrombin-activated FXIII (FXIIIA-subunit), a plasma transglutaminase, activates VEGFR-2 by crosslinking it with the alpha(v)beta(3) integrin on the surface of endothelial cells (EC), thereby stimulating angiogenesis. Tissue transglutaminase (tTG), which is functionally and structural...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2006.05.019
更新日期：2006-10-01 00:00:00
abstract：:The DNA topoisomerase I inhibitor camptothecin (CAM) is selectively cytotoxic to S-phase cells of HL-60, and some other myelogenous leukemic lines. The early effects of cell exposure to 0.05-0.2 micrograms/ml CAM are seen after 2 h; at that time a progressive degradation of DNA in the chromatin of S-phase cells is ini...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(91)90534-2
更新日期：1991-03-01 00:00:00
abstract：:We have established, in continuous cell culture, a spontaneously immortalized chicken embryo fibroblast (CEF) cell line (DF-1) as well as several other immortal CEF cell lines. The immortal DF-1 cells divided more rapidly than primary and other immortal CEF cells. To identify the genes involved in rapidly dividing DF-...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2001.5207
更新日期：2001-05-01 00:00:00
abstract：:Different extracellular matrix (ECM) molecules, when presented to hippocampal neurons in culture in a substrate-bound form, exert strikingly similar effects on the establishment of neuronal polarity, i.e., the growth of axon-like major neurites is favored, whereas extension of dendrite-like minor neurites is inhibited...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1997.3655
更新日期：1997-08-25 00:00:00
abstract：:Y-box binding protein-1 (YBX1) is a multifunctional protein and often acts as an indicator of poor prognosis in cancers. Increasing evidence has shown that the levels of YBX1 protein were closely associated with multidrug resistance, relapse, metastasis and poor prognosis in cancers. However, its role in nasopharyngea...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2017.10.009
更新日期：2017-12-01 00:00:00
abstract：:Evidence is presented that endonuclease digestion of isolated, unfixed chromosomes results in the production of banding patterns similar to those produced by digestion of fixed, air-dried chromosomes. Mouse L cell chromosomes were isolated under acidic or relatively neutral pH conditions, exposed in situ (as wet mount...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(86)90036-4
更新日期：1986-06-01 00:00:00
abstract：:Upregulated ERK1/2 activity is often correlated with AKT activation during prostate cancer (PCa) progression, yet their functional relation needs elucidation. Using androgen-deprived LNCaP cells, in which ERK1/2 activation occurs in strong correlation with AKT activation, we found that AKT-mediated B-Raf regulation is...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2013.05.008
更新日期：2013-07-15 00:00:00
abstract：:Epidermal growth factor (EGF) has been shown to be a potent mitogen for epidermal cells both in vitro and in vivo, thus contributing to the development of an organism. It has recently become clear that peroxisome proliferator-activated receptor-beta/delta (PPARbeta/delta) expression and activation is involved in the c...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2008.06.013
更新日期：2008-10-15 00:00:00
abstract：:In low serum (0.2%) medium, ascorbate stimulates primary avian tendon cells to increase procollagen synthesis from 12 to 50% of total protein synthesis. This is reversibly blocked by an increase of serum levels from 0.2 to 3%. Ascorbate in low serum medium has been shown previously to stimulate the procollagen pathway...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(88)90330-8
更新日期：1988-06-01 00:00:00
abstract：:Recruitment of mesenchymal stem cells (MSC) to tissue damages is a promising approach for in situ tissue regeneration. The physiological mechanisms and regulatory processes of MSC trafficking to injured tissue remain poorly understood. However, the pivotal role of chemokines in MSC recruitment has already been shown. ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2008.12.022
更新日期：2009-05-01 00:00:00
abstract：:Extracellular matrix (ECM) has been reported to enhance epithelial cell attachment and proliferation as well as to induce differentiation in vitro. In an attempt to determine the benefits of culturing pancreatic islet cells on ECM, we studied the morphological and functional patterns of rat islet cells and an insulin-...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/s0014-4827(85)80005-7
更新日期：1985-08-01 00:00:00
abstract：:Activated fibroblasts promote physiological wound repair following tissue injury. However, dysregulation of fibroblast activation contributes to the development of fibrosis by enhanced production and contraction of collagen-rich extracellular matrix. At the peak of their activities, fibroblasts undergo phenotypic conv...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2019.03.027
更新日期：2019-06-01 00:00:00
abstract：:To determine which endocytic compartments are sensitive to sucrose-induced osmotic swelling, CHO and Vero cells were cultured for 1-3 days in media containing 0.03 to 0.05 M sucrose. (Sucrose is internalized but not digested by these cells.) To immunolocalize late endocytic compartments, cells were fixed with formalde...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(91)90156-o
更新日期：1991-01-01 00:00:00
abstract：:The pleiotropic cytokine interleukin (IL)-10 is best characterized by its ability to downregulate inflammation and promote peripheral tolerance. On the other hand, IL-10 was also found to maintain the effector response of CD8+ T cells and promote the expansion of tumor-resident CD8+ T cells. In diffuse large B cell ly...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2017.08.036
更新日期：2017-11-15 00:00:00
abstract：:During pathological bone loss, factors that are both stimulatory and inhibitory for osteoclast differentiation are over-expressed. Despite the presence of inhibitory factors, osteoclast differentiation is significantly enhanced to bring about bone loss. To examine the hypothesis that stimulatory growth factors overcom...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2003.11.009
更新日期：2004-03-10 00:00:00
abstract：:Over the last two decades molecular studies of inherited tumor syndromes that are associated with the development of kidney cancer have led to the identification of genes and biochemical pathways, which play key roles in the malignant transformation of renal epithelial cells. Some of these findings have broad biologic...
journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2012.02.026
更新日期：2012-05-15 00:00:00
abstract：:Proliferation of human B- and T-lymphoid cell lines including Raji and Akata cells was found to be arrested at the G1 stage in the cell cycle by dimethyl sulfoxide (DMSO). The G1 arrest by DMSO occurred gradually and was completed within 96 h after addition of 1.5% DMSO concomitantly with a decrease in growth rate. Pr...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(90)90108-m
更新日期：1990-03-01 00:00:00
abstract：:In this study an amphotropic retrovirus has been used to efficiently transduce normal human (NF) and scleroderma (systemic sclerosis; SSc) dermal fibroblasts (SScF) with a sequence encoding a temperature-sensitive mutant of the SV40 large T antigen (tsA58-U19). From the primary outgrowths of skin explants, cultures we...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1995.1332
更新日期：1995-10-01 00:00:00
abstract：:Calf aortic smooth muscle (CASM) cells cultured in vitro at high cell density (4 x 10(4) cells/cm2) on bacteriological petri dishes in the presence of serum pile up in clusters and create open spaces in the monolayer. This phenomenon is clearly visible 6 days after plating and is markedly enhanced by the addition of f...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(90)90216-w
更新日期：1990-01-01 00:00:00
abstract：:In this study we have analyzed short- and long-term changes in extracellular signal-regulated kinase (ERK) 1 and 2 activity during 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced differentiation of human promyelocytic leukemia cells. Immunoprecipitation of HL-60 cellular extracts with an ERK antibody followed by in...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1997.3631
更新日期：1997-08-01 00:00:00
abstract：:In the naturally synchronous mitosis of the syncytial plasmodium of Physarum polycephalum, the nucleolus disintegrates in prophase, releasing a large amount of ribosomal RNA. Using biotinylated rDNA probes, we studied by high-resolution in situ hybridization the behavior of this nucleolar RNA throughout mitosis. Our r...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1993.1274
更新日期：1993-10-01 00:00:00
abstract：:Phorbol dibutyrate (PDBu) induced the formation of podosome-like structures together with partial disassembly of actin stress fibers in A7r5 smooth muscle cells. These podosomes contained alpha-actinin, F-actin, and vinculin and exhibit a tubular, column-like structure arising perpendicularly from the bottom of PDBu-t...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2002.5592
更新日期：2002-10-15 00:00:00
abstract：:It has been suggested that the protein kinase C zeta (zeta PKC) isoform is involved in mitogenic signaling in Xenopus oocytes and mammalian cells. Thus, the characterization of potential regulatory molecules that bind to zeta PKC is of great interest. We report here the identification by affinity chromatography of tub...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1996.3364
更新日期：1997-01-10 00:00:00
abstract：:Differentiation of endoderm into intestinal epithelium is initiated at E13.5 of mouse development when there are significant changes in morphology resulting in the conversion of undifferentiated stratified epithelium into a mature epithelial monolayer. Here we demonstrate that monolayer formation is associated with th...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2004.10.006
更新日期：2005-02-15 00:00:00
abstract：:Extraocular muscles (EOMs) are categorized as skeletal muscles; however, emerging evidence indicates that their gene expression profile, metabolic characteristics and functional properties are significantly different from the prototypical members of this muscle class. Gene expression profiling of developing and adult ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2010.03.018
更新日期：2010-07-15 00:00:00
abstract：:Chronic lymphocytic leukemia lymphocytes were used to study mechanisms involved in apoptosis (programmed cell death). Apoptosis, which was determined by morphological changes including cell death and by internucleosomal DNA fragmentation, occurred during culture for 1 to 2 days in a portion of the cells from three of ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(92)90393-m
更新日期：1992-02-01 00:00:00
abstract：:The interaction of fluorescein-labeled nerve growth factor (NGF) with human melanoma cells (A875) has been studied in order to assess better methodology for rigorous NGF binding studies. The NGF was modified at a single carboxyl group with iodoacetamidofluorescein after reaction with carbodiimide and cystamine. The mo...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1994.1012
更新日期：1994-01-01 00:00:00
abstract：:In addition to the well-characterized surface gap junctions expressed at contact sites between cells, annular gap junction profiles have been localized within the cytoplasm of some cell populations. To study and characterize these annular profiles, gap junction protein type was demonstrated with Western blot and immun...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1997.3628
更新日期：1997-08-01 00:00:00
