The zeta isozyme of protein kinase C binds to tubulin through the pseudosubstrate domain.

abstract：:Homologous desensitization of responses to bombesin in small cell lung cancer cells can be mimicked by protein kinase C-activating phorbol esters. We show that phorbol ester-induced desensitization can be blocked by prior treatment with the protein kinase C inhibitor staurosporine or prolonged pretreatment with phorbo...

journal_title：Experimental cell research

authors： Frankel A,Viallet J

更新日期：1993-12-01 00:00:00

abstract：:In this study we demonstrated that neural rosettes derived from human ES cells can give rise either to neural crest precursors, following expansion in presence of bFGF and EGF, or to dopaminergic precursors after exposure to ventralizing factors Shh and FGF8. Both regionalised precursors are capable of extensive proli...

journal_title：Experimental cell research

authors： Colleoni S,Galli C,Giannelli SG,Armentero MT,Blandini F,Broccoli V,Lazzari G

更新日期：2010-04-15 00:00:00

abstract：:A heterogeneous RNP structure has been isolated from rat liver nuclei by a method previously used for the isolation of 30S RNP complexes carrying heterogeneous RNA (hnRNA) [1]. The RNP sediments in sucrose gradients with s-values of 70-110S. Formaldehyde-fixed preparations band at Q = 1.40 in isopycnic CsCl gradients....

journal_title：Experimental cell research

authors： Hatzoglou M,Adamtziki E,Margaritis L,Sekeris CE

更新日期：1985-03-01 00:00:00

abstract：:Using L1210 murine leukemia cells, we have previously shown that in response to treatment with drugs having different targets, apoptotic cell death occurs through at least two different signaling pathways. Here, we present evidence that nuclear extracts from staurosporine-treated cells elicit DNase II activity that is...

journal_title：Experimental cell research

authors： Belmokhtar CA,Torriglia A,Counis MF,Courtois Y,Jacquemin-Sablon A,Ségal-Bendirdjian E

更新日期：2000-01-10 00:00:00

abstract：:In chick embryo fibroblasts, the mRNA for extracellular matrix protein tenascin-C is induced 2-fold by cyclic strain (10%, 0.3 Hz, 6 h). This response is attenuated by inhibiting Rho-dependent kinase (ROCK). The RhoA/ROCK signaling pathway is primarily involved in actin dynamics. Here, we demonstrate its crucial impor...

journal_title：Experimental cell research

authors： Sarasa-Renedo A,Tunç-Civelek V,Chiquet M

更新日期：2006-05-01 00:00:00

abstract：:The retention of mRNAs near the nuclei that synthesize them may be an important feature of the organization of multinucleated skeletal myotubes. Here, we assess the possible role of two factors in this localization. First, we examine the role of mRNA half-life, by studying the distribution of the mRNA for the transfer...

journal_title：Experimental cell research

authors： Ralston E,McLaren RS,Horowitz JA

更新日期：1997-11-01 00:00:00

abstract：:Noradrenaline, adrenaline, and isoproterenol induce head-to-head association in bovine spermatozoa in a Tris-HCl-buffered medium. Noradrenaline was the most and isoproterenol the least efficient. This effect was stimulated by Ca2+, Mg2+ and Mn2+, Ca2+ being more efficient than the other two ions. At 2 X 10(-6) M Ca2+,...

journal_title：Experimental cell research

authors： Lindahl PE

更新日期：1978-05-01 00:00:00

abstract：:Cell-to-cell communication is essential for the development and maintenance of multicellular organisms. The tunneling nanotube (TNT) is a recently recognized distinct type of intercellular communication device. TNTs are thin protrusions of the plasma membrane and allow direct physical connections of the plasma membran...

journal_title：Experimental cell research

authors： Kimura S,Hase K,Ohno H

更新日期：2012-08-15 00:00:00

abstract：:The products of the human Arg gene and human, mouse, Drosophila, and nematode Abl genes characterize the Abelson family of nonreceptor tyrosine protein kinase. The Arg gene, expressed as a 12-kb transcript, codes a protein highly related to c-abl in the tyrosine kinase, SH2, and SH3 domains, and both proteins have a m...

journal_title：Experimental cell research

authors： Perego RA,Bianchi C,Brando B,Urbano M,Del Monte U

更新日期：1998-11-25 00:00:00

abstract：:Interleukin-1 beta (IL-1) increases the synthesis of prostaglandins as well as the expression of synovial fluid phospholipase A2 (PLA2) mRNA and activity by chondrocytes. In order to examine the potential involvement of cyclooxygenase in the induction of prostaglandins by IL-1, the effect of IL-1 on rabbit articular c...

journal_title：Experimental cell research

authors： Lyons-Giordano B,Pratta MA,Galbraith W,Davis GL,Arner EC

更新日期：1993-05-01 00:00:00

abstract：:Recent reports suggest that several viruses, besides human immunodeficiency virus, induce apoptosis in infected cells. We report here that Sendai virus or Herpes simplex virus type 1 (HSV-1), two potent inducers of interferon-alpha, caused cell death in a consistent number of human peripheral blood mononuclear cells. ...

journal_title：Experimental cell research

authors： Tropea F,Troiano L,Monti D,Lovato E,Malorni W,Rainaldi G,Mattana P,Viscomi G,Ingletti MC,Portolani M

更新日期：1995-05-01 00:00:00

abstract：:Phorbol esters have been reported to induce opposite responses in fetal myoblasts and in satellite cells isolated from adult skeletal muscles. We examined the possibility that different levels of protein kinase C (PKC) activity and different phorbol ester binding characteristics account for these responses. For this p...

journal_title：Experimental cell research

authors： Martelly I,Gautron J,Moraczewski J

更新日期：1989-07-01 00:00:00

abstract：:K252a, a protein kinase inhibitor, acts as a neurotrophic factor in several neuronal cells. In this study we show that K252a enhanced the differentiation of C2C12 myoblasts as well as tyrosine phosphorylation of several focal adhesion-associated proteins including p130(Cas), focal adhesion kinase, and paxillin. The ty...

journal_title：Experimental cell research

authors： Lee KH,Lee SH,Kim D,Rhee S,Kim C,Chung CH,Kwon H,Kang MS

更新日期：1999-11-01 00:00:00

abstract：:After the 10th nuclear cycle the yolk centrosomes follow an irregular pathway. Unlike the somatic centrosomes, which move to the opposite poles of the nuclei to form the bipolar spindles, the yolk centrosomes remain as pairs at one pole of the yolk nuclei or shift feebly and nucleate irregular spindles, most of which ...

journal_title：Experimental cell research

authors： Callaini G,Dallai R

更新日期：1991-01-01 00:00:00

abstract：:Breast cancer (BC) is one of the most common cancers among women in both developed and developing countries with a rising incidence. Using the MMTV-PyMT transgenic mouse model and xenografted breast cancer model, we found that R5, a neutralizing antibody to Robo1, significantly inhibited BC growth and metastasis. Angi...

journal_title：Experimental cell research

authors： Li Q,Cao J,He Y,Liu X,Mao G,Wei B,Liao S,Zhang Q,Li J,Zheng L,Wang L,Qi C

更新日期：2020-02-01 00:00:00

abstract：:The first angiogenesis inhibitors for cancer have now been approved by the F.D.A. in the U.S. and in 28 other countries, including China. The majority of these are monotherapies that block VEGF. However, mutant tumor cells may over time produce redundant angiogenic factors. Therefore, for long-term use in cancer, comb...

journal_title：Experimental cell research

authors： Folkman J

更新日期：2006-03-10 00:00:00

abstract：:Polo-like kinases (Plks) are essential for progression through mitosis. The activity of these kinases peak during M phase and this activation has been attributed to phosphorylation. Kinases capable of activating Plks in vitro have been previously identified both in mammalian cells and in Xenopus laevis oocytes (SLK an...

journal_title：Experimental cell research

authors： Alves PS,Godinho SA,Tavares AA

更新日期：2006-02-01 00:00:00

abstract：:We have shown previously that the activity of the long myosin light chain kinase (MLCK) is cell cycle regulated with a decrease in specific activity during mitosis that can be restored following treatment with alkaline phosphatase. To better understand the role and significance of phosphorylation in regulating MLCK fu...

journal_title：Experimental cell research

authors： Dulyaninova NG,Bresnick AR

更新日期：2004-10-01 00:00:00

abstract：:The characterization of a mucus-producing human cell line (HC-84) derived from a colon carcinoma and its response to in vitro reserpine treatment is reported. Mucous granules were demonstrated within these cells on the basis of electron microscopic examination and incorporation of [3H]glucosamine with subsequent autor...

journal_title：Experimental cell research

authors： Brady RC,Karnaky KJ Jr,Dedman JR

更新日期：1984-01-01 00:00:00

abstract：:Murine embryonal carcinoma F9 cells can be induced to differentiate by 2-difluoromethylornithine (DFMO), an irreversible inhibitor of ornithine decarboxylase (ODC). The differentiated phenotype is similar to that of retinoic acid (RA)-treated F9 cells. In contrast to F9 cells the differentiated cells secrete plasminog...

journal_title：Experimental cell research

authors： Jetten AM,Shirley JE

更新日期：1985-01-01 00:00:00

abstract：:The Golgi apparatus plays roles in cell polarity, directional cell migration, and bipolar spindle assembly, as well as the secretary pathway. In addition, recent studies have suggested the Golgi-dependent control of mitotic entry. We studied the role of the centrosomal kinase Aurora A in maintaining the Golgi apparatu...

journal_title：Experimental cell research

authors： Kimura M,Takagi S,Nakashima S

更新日期：2018-06-01 00:00:00

abstract：:During pathological bone loss, factors that are both stimulatory and inhibitory for osteoclast differentiation are over-expressed. Despite the presence of inhibitory factors, osteoclast differentiation is significantly enhanced to bring about bone loss. To examine the hypothesis that stimulatory growth factors overcom...

journal_title：Experimental cell research

authors： Gorny G,Shaw A,Oursler MJ

更新日期：2004-03-10 00:00:00

abstract：:The von Hippel-Lindau (VHL) protein protects microtubules (MTs) from destabilization by nocodazole treatment. Based on this fixed-cell assay with static end points, VHL has been reported to directly stabilize the MT cytoskeleton. To investigate the dynamic changes in MTs induced by VHL in living cells, we measured the...

journal_title：Experimental cell research

authors： Lolkema MP,Mehra N,Jorna AS,van Beest M,Giles RH,Voest EE

更新日期：2004-12-10 00:00:00

abstract：:Small vertebrate organisms have emerged as key players in the post-genomic era for the functional characterization of novel genes on a high-throughput scale. In this context, the zebrafish embryos and Xenopus tadpoles represent attractive and valuable models to rapidly identify and characterize novel genes involved in...

journal_title：Experimental cell research

authors： Ny A,Autiero M,Carmeliet P

更新日期：2006-03-10 00:00:00

abstract：:Impaired elastin fiber assembly is observed in the fetal ductus arteriosus (DA), associated with a reduced concentration of elastin binding protein (EBP), a 67-kDa galactolectin. It is also seen in cultured aortic (Ao) smooth muscle cells (SMC) following the release of the EBP by glycosaminoglycans rich in N-acetylgal...

journal_title：Experimental cell research

authors： Hinek A,Boyle J,Rabinovitch M

更新日期：1992-12-01 00:00:00

abstract：:Fluid-phase endocytosis was significantly faster in three lines of human lysosomal storage disease fibroblasts (two I cells and one Sandhoff's) than in three normals. Re-exocytosis of fluid showed similar kinetics in all lines when release was expressed as a percentage of the radioactivity associated with the cells at...

journal_title：Experimental cell research

authors： Dean RT

更新日期：1984-04-01 00:00:00

abstract：:The large zinc finger protein KRC regulates transcription of target genes via the kappaB gene enhancer element. As an attempt to investigate the cellular function of KRC, we have established cell lines stably transfected with KRC expression vectors. Introduction of a vector directing expression of a transcript antisen...

journal_title：Experimental cell research

authors： Allen CE,Wu LC

更新日期：2000-11-01 00:00:00

abstract：:Distal-less homeobox 5 (Dlx5) is a pro-osteogenic but anti-adipogenic transcription factor that regulates lineage commitment in mesenchymal stem cells. Although the expression of Dlx5 is known to be decreased by adipogenic stimuli, the mechanism of Dlx5 down-regulation has not yet been clarified. MicroRNAs (miRNAs) ar...

journal_title：Experimental cell research

authors： Qadir AS,Woo KM,Ryoo HM,Baek JH

更新日期：2013-08-15 00:00:00

abstract：:A respiratory epithelial cell line M3E3/C3, derived from fetal Syrian hamster lung, grew very slowly in RPMI 1640 medium with serum concentrations as low as 1.5%. Under these conditions the simultaneous action of epidermal growth factor (EGF) and vitamin A (retinol) was apparently a combination of growth enhancement a...

journal_title：Experimental cell research

authors： Böning W,Kakunaga T,Emura M,Mohr U

更新日期：1986-03-01 00:00:00

abstract：:Phosphatidylinositol 3-kinase inhibitors have been shown to affect endocytosis or subsequent intracellular sorting in various receptor systems. Agonist-activated beta(2)-adrenergic receptors undergo desensitization by mechanisms that include the phosphorylation, endocytosis and degradation of receptors. Following endo...

journal_title：Experimental cell research

authors： Awwad HO,Iyer V,Rosenfeld JL,Millman EE,Foster E,Moore RH,Knoll BJ

更新日期：2007-07-15 00:00:00