Inhibition of ornithine decarboxylase by retinoic acid and difluoromethylornithine in relation to their effects on differentiation and proliferation.

abstract：:Transdifferentiation of vascular smooth muscle cells (VSMC) promotes the development of vascular calcifications such as arteriosclerosis. The aim was to investigate effects of specific extracellular matrix (ECM) components on transdifferentiation of VSMC to identify novel ECM-based therapeutic tools. Human collagens I...

journal_title：Experimental cell research

authors： Freise C,Bobb V,Querfeld U

更新日期：2017-09-15 00:00:00

abstract：:Type VI collagen is a nonfibrillar collagen present as a network throughout the chick secondary stroma. Immunolocalization of type VI collagen both in the chick corneal stroma and in other systems demonstrates that type VI collagen is present associated with cells and between striated fibrils. We hypothesize that type...

journal_title：Experimental cell research

authors： Doane KJ,Yang G,Birk DE

更新日期：1992-06-01 00:00:00

abstract：:In most existing transgenic mouse models developed for the study of specific genes in the skin, the goal has been to target transgene expression to defined populations of cells in the cutaneous epithelium. Keratin promoters have been especially useful for this purpose. In some instances, however, it may be desirable t...

journal_title：Experimental cell research

authors： Sawicki JA,Morris RJ,Monks B,Sakai K,Miyazaki J

更新日期：1998-10-10 00:00:00

abstract：:The abilities of eight extracellular matrix proteins, fibronectin, vitronectin, laminin, and collagen types I, II, III, IV, and V to bind insulin were examined by binding studies with insulin conjugated with peroxidase. At a physiological pH and ionic strength, type V collagen bound to insulin most strongly. The other...

journal_title：Experimental cell research

authors： Yaoi Y,Hashimoto K,Takahara K,Kato I

更新日期：1991-06-01 00:00:00

abstract：:The pleiotropic cytokine interleukin (IL)-10 is best characterized by its ability to downregulate inflammation and promote peripheral tolerance. On the other hand, IL-10 was also found to maintain the effector response of CD8+ T cells and promote the expansion of tumor-resident CD8+ T cells. In diffuse large B cell ly...

journal_title：Experimental cell research

authors： Qiu H,Hu X,Gao L,Chen L,Chen J,Yuan J,Huang C,Xu X,Yang J

更新日期：2017-11-15 00:00:00

abstract：:The distribution and organisation of F-actin during the cell cycle of meristematic root-tip cells of Allium was investigated using a rhodamine-labelled phalloidin to stain F-actin in isolated cell preparations. Such preparations could, in addition, be stained for tubulin by immunofluorescence, enabling a comparison be...

journal_title：Experimental cell research

authors： Clayton L,Lloyd CW

更新日期：1985-01-01 00:00:00

abstract：:Rap proteins (Rap1, Rap2a, b, c) are small molecular weight GTPases of the Ras family. Rap G proteins mediate diverse cellular events such as cell adhesion, proliferation, and gene activation through various signaling pathways. Activation of Rap signal is regulated tightly by several specific regulatory proteins inclu...

journal_title：Experimental cell research

authors： Minato N

更新日期：2013-09-10 00:00:00

abstract：:Cell surface beta-1,4-galactosyltransferase (GalTase) partially mediates a variety of cell interactions with laminin-containing matrices, including mesenchymal cell spreading and migration and neurite initiation, by binding to N-linked oligosaccharides within the E8 domain of laminin. Previous studies using indirect i...

journal_title：Experimental cell research

authors： Eckstein DJ,Shur BD

更新日期：1992-07-01 00:00:00

abstract：:A method for isolation of C-cells from rat fetuses was developed, and the morphological plasticity of the cells in primary culture systems was tested. Thyroid-parathyroid-ultimobranchial body (UB) complexes from 16-day rat fetuses were treated with 0.1% collagenase and 1000 PU/ml Dispase at 37 degrees C for 1 h. After...

journal_title：Experimental cell research

authors： Nishiyama I,Fujii T

更新日期：1992-02-01 00:00:00

abstract：:Nuclear and territorial positioning of p- and q-telomeres and centromeres of chromosomes 3, 8, 9, 13, and 19 were studied by repeated fluorescence in situ hybridization, high-resolution cytometry, and three-dimensional image analysis in human blood lymphocytes before and after stimulation. Telomeres were found on the ...

journal_title：Experimental cell research

authors： Amrichová J,Lukásová E,Kozubek S,Kozubek M

更新日期：2003-09-10 00:00:00

abstract：:In order to test the existence of mechanical coupling between the rotational movements of two adjacent nuclei, we prepared binucleate 3T3 cells and observed their nuclear movements by near infrared microscopy and recorded them with time-lapse video techniques. We found that 49 out of 110 (44%) of the selected binuclea...

journal_title：Experimental cell research

authors： Paddock SW,Albrecht-Buehler G

更新日期：1986-09-01 00:00:00

abstract：:DNA methylation in F9 embryonal carcinoma cells at various stages of retinoic acid-induced differentiation was compared to that in immortalized differentiated derivatives of this cell line. Different repetitive sequences, such as L1 LINE, GAPDH pseudogenes, and major and minor satellite DNA, lost their methylation to ...

journal_title：Experimental cell research

authors： Teubner B,Schulz WA

更新日期：1994-02-01 00:00:00

abstract：:We report that prosaposin binds to U937 and is active as a protective factor on tumor necrosis factor alpha (TNFalpha)-induced cell death. The prosaposin-derived saposin C binds to U937 cells in a concentration-dependent manner, suggesting that prosaposin behaves similarly. Prosaposin binding induces U937 cell death p...

journal_title：Experimental cell research

authors： Misasi R,Garofalo T,Di Marzio L,Mattei V,Gizzi C,Hiraiwa M,Pavan A,Grazia Cifone M,Sorice M

更新日期：2004-08-01 00:00:00

abstract：:In the human lymphoblastic cell line KE 37, Northern blot analysis with cDNA probes for human regulatory subunits RII alpha RII beta of the cAMP-dependent protein kinase (A-kinase) type II and immunoblotting or immunoprecipitation studies with several antibodies directed against RII alpha and RII beta show that these ...

journal_title：Experimental cell research

authors： Keryer G,Rios RM,Landmark BF,Skalhegg B,Lohmann SM,Bornens M

更新日期：1993-02-01 00:00:00

abstract：:The base at the first anticodon ("wobble") position of certain eukaryotic tRNA species is either guanine or the hypermodified base queuine. These tRNA species are synthesized with guanine in the wobble position (tRNAG); this guanine can then be replaced with queuine by the action of the enzyme tRNA-guanine ribosyltran...

journal_title：Experimental cell research

authors： Parniak MA,Andrejchyshyn S,Marx S,Kleiman L

更新日期：1991-07-01 00:00:00

abstract：:Proteolysis of corneodesmosin, a 52- to 56-kDa basic protein located in the extracellular part of the modified desmosomes (corneodesmosomes) of human cornified epithelia, is thought to be a key event of desquamation. Three monoclonal antibodies specific for human corneodesmosin were used to search for the expression o...

journal_title：Experimental cell research

authors： Montézin M,Simon M,Guerrin M,Serre G

更新日期：1997-02-25 00:00:00

abstract：:During skeletal muscle development three intermediate filament proteins are expressed: nestin, vimentin, and desmin. Vimentin and desmin belong to the class III intermediate filaments and are closely related to each other, whereas nestin is a more distantly related, class VI, intermediate filament. It was previously o...

journal_title：Experimental cell research

authors： Sjöberg G,Jiang WQ,Ringertz NR,Lendahl U,Sejersen T

更新日期：1994-10-01 00:00:00

abstract：:Fibroblast migration is a central process in skin wound healing, which requires the coordination of several types of growth factors. bFGF, a well-known fibroblast growth factor (FGF), is able to accelerate fibroblast migration; however, the underlying mechanism of bFGF regulation fibroblast migration remains unclear. ...

journal_title：Experimental cell research

authors： Zhu ZX,Sun CC,Ting Zhu Y,Wang Y,Wang T,Chi LS,Cai WH,Zheng JY,Zhou X,Cong WT,Li XK,Jin LT

更新日期：2017-06-15 00:00:00

abstract：:The casein kinase I (CKI) family consists of at least seven vertebrate genes, some of which can be alternatively spliced. Previously, we have studied the four splice variants of the chicken CKIalpha gene. The four proteins differ only by the presence or absence of two peptides, a 28-amino-acid "L" insert in the cataly...

journal_title：Experimental cell research

authors： Fu Z,Chakraborti T,Morse S,Bennett GS,Shaw G

更新日期：2001-10-01 00:00:00

abstract：:The fruit-fly Drosophila melanogaster harbours different types of ciliary structures: ciliary projections associated with neurons of type I and cilium-like regions (CLRs) found during male gametogenesis. The latter deserve particular attention since they are morphologically similar to vertebrate primary cilia and tran...

journal_title：Experimental cell research

authors： Gottardo M,Persico V,Callaini G,Riparbelli MG

更新日期：2018-10-01 00:00:00

abstract：:Protein kinase B (Akt1) holds a central role for cellular growth, development, and survival, but the cellular pathways of Akt1 that prevent inflammatory demise in the vascular system remain undefined. Employing a constitutively active form of Akt1 (myristoylated Akt1) in endothelial cells (ECs), we demonstrate that Ak...

journal_title：Experimental cell research

authors： Chong ZZ,Kang JQ,Maiese K

更新日期：2004-06-10 00:00:00

abstract：:Sertoli cells of testis belong to a unique type of polarized epithelial cells and are essential for spermatogenesis. They form the blood-testis barrier at the base of seminiferous tubule. Their numerous long, microtubule-rich processes extend inward and associate with developing germ cells to sustain germ cell growth ...

journal_title：Experimental cell research

authors： Wang F,Zhang Q,Cao J,Huang Q,Zhu X

更新日期：2008-01-01 00:00:00

abstract：:The ocular surface shares many characteristics with mucosal surfaces. In both, healing is regulated by peptide growth factors, cytokines, and extracellular matrix proteins. However, these factors are not sufficient to ensure most rapid healing. Trefoil peptides are abundantly expressed epithelial cell products which e...

journal_title：Experimental cell research

authors： Göke MN,Cook JR,Kunert KS,Fini ME,Gipson IK,Podolsky DK

更新日期：2001-04-01 00:00:00

abstract：:dUTP nucleotidohydrolase (dUTPase) catalyzes the hydrolysis of dUTP to dUMP and pyrophosphate and is the central regulator of cellular dUTP pools. Nuclear (DUT-N) and mitochondrial (DUT-M) isoforms of the protein have been identified in humans and arise from the same gene by the alternative use of 5' exons. Recently, ...

journal_title：Experimental cell research

authors： Tinkelenberg BA,Fazzone W,Lynch FJ,Ladner RD

更新日期：2003-07-01 00:00:00

abstract：:Neuroblastoma is a childhood tumor with a poor survival in advanced stage disease despite intensive chemotherapeutic regimes. The new histone deacetylase (HDAC) inhibitor BL1521 has shown promising results in neuroblastoma. Inhibition of HDAC resulted in a decrease in proliferation and metabolic activity, induction of...

journal_title：Experimental cell research

authors： de Ruijter AJ,Meinsma RJ,Bosma P,Kemp S,Caron HN,van Kuilenburg AB

更新日期：2005-10-01 00:00:00

abstract：:Thyroid hormone (T3) and estradiol (Est) modulate biological processes by binding to nuclear receptor proteins that, through interactions with specific response elements in the regulatory regions of genes, modulate gene transcription. Est stimulation of estrogen receptor (ER)-positive breast carcinoma cell growth occu...

journal_title：Experimental cell research

authors： Shao ZM,Sheikh MS,Rishi AK,Dawson MI,Li XS,Wilber JF,Feng P,Fontana JA

更新日期：1995-05-01 00:00:00

abstract：:A prominent secretory glycoprotein was detected in the culture medium of porcine thyrocytes which was identified as clusterin by microsequencing. Treatment of thyrocytes with thyroid stimulating hormone revealed a tight regulation of both synthesis and secretion of clusterin, with a distinct fraction of clusterin bein...

journal_title：Experimental cell research

authors： Lemansky P,Brix K,Herzog V

更新日期：1999-08-25 00:00:00

abstract：:Incubating conditions which induced actin paracrystal-like intracellular structures (actin rods) were investigated by using several cell lines. We have found that an incubation of cells of a mouse fibroblastic cell line, C3H-2K, in an isotonic solution of NaCl containing 1 mM MgCl2, 1 mM CaCl2 and 10 mM MES, pH 6.5, i...

journal_title：Experimental cell research

authors： Iida K,Yahara I

更新日期：1986-06-01 00:00:00

abstract：:Podosome formation in vascular smooth muscle cells is characterized by the recruitment of AFAP-110, p190RhoGAP, and cortactin, which have specific roles in Src activation, local down-regulation of RhoA activity, and actin polymerization, respectively. However, the molecular mechanism that underlies their specific recr...

journal_title：Experimental cell research

authors： Crimaldi L,Courtneidge SA,Gimona M

更新日期：2009-09-10 00:00:00

abstract：:We have previously established the malignant T lymphoma CS-21 cell line from a spontaneous lymphoma in a BALB/c mouse. CS-21 lymphoma cells grew continuously when they were cocultured with stromal CA-12 cells prepared from lymph nodes. CS-21 lymphoma cells, however, could not proliferate by themselves, and they underw...

journal_title：Experimental cell research

authors： Kataoka S,Naito M,Fujita N,Ishii H,Ishii S,Yamori T,Nakajima M,Tsuruo T

更新日期：1993-08-01 00:00:00