The "transition zone" of the cilium-like regions in the Drosophila spermatocytes and the role of the C-tubule in axoneme assembly.

abstract：:Replication protein A (RPA), a stable complex of three polypeptides, is the single-stranded DNA-binding protein essential for DNA replication in eukaryotic cells. Previous studies of the subcellular distribution and stability of the RPA heterotrimer during the mammalian cell cycle have produced conflicting results. He...

journal_title：Experimental cell research

authors： Dimitrova DS,Gilbert DM

更新日期：2000-02-01 00:00:00

abstract：:We have reported that PAP-1, a product of a causative gene for autosomal retinitis pigmentosa, plays a role in splicing. In this study, CIR, a protein originally identified as a CBF1-interacting protein and reported to act as a transcriptional corepressor, was identified as a PAP-1 binding protein and its function as ...

journal_title：Experimental cell research

authors： Maita H,Kitaura H,Ariga H,Iguchi-Ariga SM

更新日期：2005-02-15 00:00:00

abstract：:Xenopus oocytes carry IGF-I receptors, and undergo meiotic maturation in response to binding of IGF-I or insulin to the IGF-I receptor. Maturation is initiated upon activation of the IGF-I receptor tyrosine kinase and requires tyrosine dephosphorylation of p34cdc2, the kinase component of maturation promoting factor (...

journal_title：Experimental cell research

authors： Hainaut P,Giorgetti S,Kowalski A,Ballotti R,Van Obberghen E

更新日期：1991-07-01 00:00:00

abstract：:We found that a transient rise in cGMP levels, which was closely associated with the Ca2+ influx, occurred concomitant with the onset of myoblast fusion. The Ca2+ channel blocker D600 decreased both the cell fusion and the normal rise in cGMP levels. In contrast, the Ca2+ ionophore A23187 transiently increased cGMP le...

journal_title：Experimental cell research

authors： Choi SW,Baek MY,Kang MS

更新日期：1992-03-01 00:00:00

abstract：:In this study we have analyzed short- and long-term changes in extracellular signal-regulated kinase (ERK) 1 and 2 activity during 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced differentiation of human promyelocytic leukemia cells. Immunoprecipitation of HL-60 cellular extracts with an ERK antibody followed by in...

journal_title：Experimental cell research

authors： Meighan-Mantha RL,Wellstein A,Riegel AT

更新日期：1997-08-01 00:00:00

abstract：:Mouse embryonic stem (ES) cells are isolated from the inner cell mass (ICM)/epiblast of preimplantation embryos and are widely used in cell differentiation studies. We have previously observed differences in transcript and antigen expression following differentiation of ES cells lines in vitro. We have investigated th...

journal_title：Experimental cell research

authors： Ward CM,Barrow KM,Stern PL

更新日期：2004-02-15 00:00:00

abstract：:Cytostatic factor (CSF), found in the cytoplasm of unfertilized eggs of amphibians, causes metaphase arrest when microinjected into cleaving blastomeres. Although CSF from Rana pipiens eggs has been extracted and characterized, little is known about CSF extracted from eggs of other species. We investigated the conditi...

journal_title：Experimental cell research

authors： Moses RM,Masui Y

更新日期：1990-01-01 00:00:00

abstract：:The products of the human Arg gene and human, mouse, Drosophila, and nematode Abl genes characterize the Abelson family of nonreceptor tyrosine protein kinase. The Arg gene, expressed as a 12-kb transcript, codes a protein highly related to c-abl in the tyrosine kinase, SH2, and SH3 domains, and both proteins have a m...

journal_title：Experimental cell research

authors： Perego RA,Bianchi C,Brando B,Urbano M,Del Monte U

更新日期：1998-11-25 00:00:00

abstract：:Local regulation of the cortical cytoskeleton controls cell surface dynamics. GAP-43 and MARCKS are two abundant cytosolic protein kinase C substrates that are anchored to the cell membrane via acyl groups and interact with the cortical cytoskeleton. Each of them has been implicated in several forms of motility involv...

journal_title：Experimental cell research

authors： Wiederkehr A,Staple J,Caroni P

更新日期：1997-10-10 00:00:00

abstract：:Human, Chinese hamster or Chinese hamster/human hybrid cytoplasts were fused with UV-irradiated xeroderma pigmentosum group A (XP-A) cells. Unscheduled DNA synthesis (UDS) of the XP-A nucleus was measured 0-2 and 2-4 h after seeding of the fused population. Human cytoplasts did correct the defect in the XP-A nucleus i...

journal_title：Experimental cell research

authors： Keijzer W,Stefanini M,Bootsma D,Verkerk A,Geurts van Kessel AH,Jongkind JF,Westerveld A

更新日期：1987-04-01 00:00:00

abstract：:Oligomerization of Fas receptor by its ligand, FasL, activates a signaling cascade that leads to apoptosis of Fas bearing cells. Interestingly, many epithelia coexpress Fas and FasL, yet FasL does not trigger Fas present on the same or neighboring cells to induce spontaneous apoptosis. Here, we show that Fas and FasL ...

journal_title：Experimental cell research

authors： Tan KH,Hunziker W

更新日期：2003-04-01 00:00:00

abstract：:Formation of the neural tube is the morphological hallmark for development of the embryonic central nervous system (CNS). Therefore, neural tube development is a crucial step in the neurulation process. Slit/Robo signaling was initially identified as a chemo-repellent that regulated axon growth cone elongation, but it...

journal_title：Experimental cell research

authors： Wang G,Li Y,Wang XY,Han Z,Chuai M,Wang LJ,Ho Lee KK,Geng JG,Yang X

更新日期：2013-05-01 00:00:00

abstract：:Apoptosis plays an important role in eliminating dysfunctional damaged cells. For skin, the best characterized injurious environmental agent is ultraviolet (UV) irradiation. Most of the damaging UV irradiation is absorbed in the epidermis and leads to apoptosis of keratinocytes. However, epidermal melanocytes appear t...

journal_title：Experimental cell research

authors： Zhai S,Yaar M,Doyle SM,Gilchrest BA

更新日期：1996-05-01 00:00:00

abstract：:NIH3T3 cells carrying a dominant negative H-ras mutant 116Y acquired resistance to transformation by some PTK oncogenes, i.e., v-fes, v-abl, and v-fms, but were sensitive to viral ras and serine threonine kinase oncogenes, v-raf and v-mos. One clone, designated 1-20, infected with v-fes (1-20 fes) exhibited flat morph...

journal_title：Experimental cell research

authors： Ogiso Y,Yokoyama T,Watari H,Shih TY,Kuzumaki N

更新日期：1993-10-01 00:00:00

abstract：:Gastric cancer tissue-derived mesenchymal stem cells (GC-MSCs) are important resident stromal cells in the tumor microenvironment (TME) and have been shown to play a key role in gastric cancer progression. Whether GC-MSCs exert a tumor-promoting function by affecting anti-tumor immunity is still unclear. In this study...

journal_title：Experimental cell research

authors： Wang M,Chen B,Sun XX,Zhao XD,Zhao YY,Sun L,Xu CG,Shen B,Su ZL,Xu WR,Zhu W

更新日期：2017-12-01 00:00:00

abstract：:During the polyadenylation of pre-mRNA in vitro, poly(A) binding protein II (PAB II) binds to the growing poly(A) tail, stimulating its extension. The subcellular localization of PAB II was investigated with an antibody affinity-purified from rabbit serum raised against the purified protein. Immunofluorescence microsc...

journal_title：Experimental cell research

authors： Krause S,Fakan S,Weis K,Wahle E

更新日期：1994-09-01 00:00:00

abstract：:In this study, we demonstrate the protective effects of Cycloartenyl ferulate (CF) against Paraquat (PQ)-induced cytotoxicity and elucidate the underlying molecular mechanisms. The results show that, CF could reverse the PQ-induced growth inhibition and release of lactate dehydrogenase in HK-2 human proximal tubular c...

journal_title：Experimental cell research

authors： Hong GL,Liu JM,Zhao GJ,Wang L,Liang G,Wu B,Li MF,Qiu QM,Lu ZQ

更新日期：2013-11-01 00:00:00

abstract：:Advanced stage neuroblastomas (NB) exhibit a tissue inhibitor of metalloproteinase (TIMP)-2/matrix metalloproteinase (MMP) imbalance, considered a prerequisite for MMP involvement in tumor progression in vivo. Human SH-SY5Y NB cells exhibit a similar TIMP-2/MMP imbalance that promotes in vitro invasive behavior that i...

journal_title：Experimental cell research

authors： Cappabianca L,Farina AR,Tacconelli A,Mantovani R,Gulino A,Mackay AR

更新日期：2003-06-10 00:00:00

abstract：:Cells with and without hypoxanthine-guanine phosphoribosyltransferase (HGPRT) activity were used to examine the transfer of purine metabolites through the medium and via cell contacts. HGPRT- Chinese hamster and human fibroblasts were able to incorporate 3H-labeled purine metabolite(s) from medium in which mouse HGPRT...

journal_title：Experimental cell research

authors： Bols NC,Mosser DD,Boliska SA

更新日期：1987-11-01 00:00:00

abstract：:We have previously demonstrated that transient blocking of DNA synthesis with drugs such as 1-beta-D-arabinofuranosylcytosine results in some segments of the chromosomal DNA being replicated more than once in a single cell cycle. One explanation for this phenomenon might be that it is due to a perturbation of the cell...

journal_title：Experimental cell research

authors： Woodcock DM,Crowther PJ,Allan G,Cooper IA

更新日期：1983-08-01 00:00:00

abstract：:The p53-regulated stress-inducible gene GADD45 has been shown to participate in cellular response to DNA damage, including cell cycle checkpoint, apoptosis, and DNA repair. However, the regulation of GADD45 expression is complex and may involve both p53-dependent and -independent pathways. Recent findings have demonst...

journal_title：Experimental cell research

authors： Tong T,Fan W,Zhao H,Jin S,Fan F,Blanck P,Alomo I,Rajasekaran B,Liu Y,Holbrook NJ,Zhan Q

更新日期：2001-09-10 00:00:00

abstract：:The proline analogs cis-4-hydroxy-L-proline, 3,4-dehydro-L-proline, and 2-azetidinecarboxylic acid induced increases in the migration rate of retinal capillary endothelial cells, aortal endothelial cells, corneal endothelial cells, aortal smooth muscle cells, and retinal pericytes. cis-4-hydroxy-D-proline did not. The...

journal_title：Experimental cell research

authors： McAuslan BR,Reilly W,Hannan GN,Schindhelm K,Milthorpe B,Saur BA

更新日期：1988-06-01 00:00:00

abstract：:We have searched for brain-specific extracellular molecules using a library of monoclonal antibodies against surface antigens of differentiated PC12h cells. One of the monoclonal antibodies, PCH42-14, recognized a 27/26-kDa protein of 10-week-old rat brain on immunoblotting. PCH42-14 antigen was detected only in brain...

journal_title：Experimental cell research

authors： Ishikawa M,Kuroda Y,Kobayashi K,Sawada H,Hayashi M

更新日期：1991-03-01 00:00:00

abstract：:Binding of integrins to the extracellular matrix (ECM) activates various signal transduction pathways and regulates gene expression in many cell types. Integrin-dependent cytoplasmic protein/protein interactions are necessary for activation of those signal transduction cascades. In our studies we investigated a possib...

journal_title：Experimental cell research

authors： Danker K,Gabriel B,Heidrich C,Reutter W

更新日期：1998-03-15 00:00:00

abstract：:The insoluble cytoskeletal material remaining after detergent lysis of 'Normal' Murine Mammary Gland (NMuMG) cells, growing on plastic or collagen gel substrata, was analyzed by two-dimensional gel electrophoresis. The identity of the cytoskeletal elements was determined by their solubility properties, electrophoretic...

journal_title：Experimental cell research

authors： Hall HG,Bissell MJ

更新日期：1986-02-01 00:00:00

abstract：:Micro(mi)RNAs play important and varied roles in tumorigenesis; however, the full repertoire of miRNAs that affect cancer cell growth is not known. In this study, an miRNA library was screened to identify those that affect the growth of A549 tumor cells. Among 300 miRNAs, miR-28-5p, -323-5p, -510-5p, -552-3p, and -608...

journal_title：Experimental cell research

authors： Choi YC,Yoon S,Byun Y,Lee G,Kee H,Jeong Y,Yoon J,Baek K

更新日期：2015-12-10 00:00:00

abstract：:In this study we demonstrated that neural rosettes derived from human ES cells can give rise either to neural crest precursors, following expansion in presence of bFGF and EGF, or to dopaminergic precursors after exposure to ventralizing factors Shh and FGF8. Both regionalised precursors are capable of extensive proli...

journal_title：Experimental cell research

authors： Colleoni S,Galli C,Giannelli SG,Armentero MT,Blandini F,Broccoli V,Lazzari G

更新日期：2010-04-15 00:00:00

abstract：:To measure the adhesion of cells expressing the neural cell adhesion molecule N-CAM, mouse Lmtk- fibroblast cells were transfected by a calcium phosphate precipitation technique with eucaryotic expression vectors encoding N-CAM polypeptides. We obtained cell lines expressing the 140-kDa transmembrane isoform of N-CAM ...

journal_title：Experimental cell research

authors： Woo MK,Murray BA

更新日期：1993-02-01 00:00:00

abstract：:The expression level of DNA polymerase beta was determined in various mouse tissues. Northern blot hybridization analysis using rat cDNA as a probe revealed that the mRNA of about 1.5 kb for this enzyme is present in all kinds of tissues examined, but its content widely varies among tissues; the most abundant DNA poly...

journal_title：Experimental cell research

authors： Hirose F,Hotta Y,Yamaguchi M,Matsukage A

更新日期：1989-03-01 00:00:00

abstract：:PHOX2B and its paralogue gene PHOX2A are two homeodomain proteins in the network regulating the development of autonomic ganglia that have been associated with the pathogenesis of neuroblastoma (NB), because of their over-expression in different NB cell lines and tumour samples. We used the SK-N-BE(2)C cell line to sh...

journal_title：Experimental cell research

authors： Di Lascio S,Saba E,Belperio D,Raimondi A,Lucchetti H,Fornasari D,Benfante R

更新日期：2016-03-01 00:00:00