Abstract：
:In this study, we demonstrate the protective effects of Cycloartenyl ferulate (CF) against Paraquat (PQ)-induced cytotoxicity and elucidate the underlying molecular mechanisms. The results show that, CF could reverse the PQ-induced growth inhibition and release of lactate dehydrogenase in HK-2 human proximal tubular cells. Treatment with PQ induced apoptosis in HK-2 cells, as evidenced by accumulation of sub-G1 cell population, chromatin condensation, DNA fragmentation, and translocation of phosphatidylserine, which were significantly attenuated by co-incubation with CF. Mitochondria-mediated apoptosis pathway contributed importantly to PQ-induced apoptosis, as revealed by the activation of caspase-3/-9, cleavage of PARP, depletion of mitochondrial membrane potential regulated by Bcl-2 family members, and overproduction of reactive oxygen species, which were also effectively blocked by CF. Moreover, treatments of PQ strongly inhibited the expression of Nrf2 and the downstream effectors, HO1 and NQO1. However, co-treatment with CF effectively reversed this action of PQ. Furthermore, silencing of Nrf2 by the siRNA technique significantly blocked the cytoprotective effects of CF against PQ-induced apoptosis, which suggest the important role of Nrf2 signaling pathway an cell apoptosis induced by PQ. Taken together, this study provides a novel strategy for molecular intervention against PQ-induced nephrotoxicity by using phytochemicals.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Hong GL,Liu JM,Zhao GJ,Wang L,Liang G,Wu B,Li MF,Qiu QM,Lu ZQdoi
10.1016/j.yexcr.2013.08.005subject
Has Abstractpub_date
2013-11-01 00:00:00pages
2845-55issue
18eissn
0014-4827issn
1090-2422pii
S0014-4827(13)00334-0journal_volume
319pub_type
杂志文章abstract：:The ccl mutation in Paramecium tetraurelia reversibly and rapidly blocks cell cycle progression and DNA synthesis at the restrictive temperature. Progression through the cell cycle is blocked during both the G1 and S portions of the cell cycle, while at the restrictive temperature there is neither residual cell cycle ...
journal_title：Experimental cell research
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journal_title：Experimental cell research
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journal_title：Experimental cell research
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journal_title：Experimental cell research
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journal_title：Experimental cell research
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journal_title：Experimental cell research
pub_type： 杂志文章
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1993.1287
更新日期：1993-11-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章,评审
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doi：10.1016/j.yexcr.2006.01.032
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journal_title：Experimental cell research
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doi：10.1016/j.yexcr.2008.11.020
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journal_title：Experimental cell research
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doi：10.1016/j.yexcr.2015.03.001
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journal_title：Experimental cell research
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pub_type： 杂志文章
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journal_title：Experimental cell research
pub_type： 杂志文章,评审
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journal_title：Experimental cell research
pub_type： 杂志文章
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journal_title：Experimental cell research
pub_type： 杂志文章
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abstract：:A new beta 1-containing integrin was isolated from rat liver by affinity chromatography on Sepharose conjugated with the peptide GRGDSPC. The interaction was weakened but not abolished when the arginine and/or aspartic acid in the peptide were replaced with lysine and glutamic acid, respectively. In contrast, the cyst...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1994.1189
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