The reversal of paraquat-induced mitochondria-mediated apoptosis by cycloartenyl ferulate, the important role of Nrf2 pathway.


:In this study, we demonstrate the protective effects of Cycloartenyl ferulate (CF) against Paraquat (PQ)-induced cytotoxicity and elucidate the underlying molecular mechanisms. The results show that, CF could reverse the PQ-induced growth inhibition and release of lactate dehydrogenase in HK-2 human proximal tubular cells. Treatment with PQ induced apoptosis in HK-2 cells, as evidenced by accumulation of sub-G1 cell population, chromatin condensation, DNA fragmentation, and translocation of phosphatidylserine, which were significantly attenuated by co-incubation with CF. Mitochondria-mediated apoptosis pathway contributed importantly to PQ-induced apoptosis, as revealed by the activation of caspase-3/-9, cleavage of PARP, depletion of mitochondrial membrane potential regulated by Bcl-2 family members, and overproduction of reactive oxygen species, which were also effectively blocked by CF. Moreover, treatments of PQ strongly inhibited the expression of Nrf2 and the downstream effectors, HO1 and NQO1. However, co-treatment with CF effectively reversed this action of PQ. Furthermore, silencing of Nrf2 by the siRNA technique significantly blocked the cytoprotective effects of CF against PQ-induced apoptosis, which suggest the important role of Nrf2 signaling pathway an cell apoptosis induced by PQ. Taken together, this study provides a novel strategy for molecular intervention against PQ-induced nephrotoxicity by using phytochemicals.


Exp Cell Res


Hong GL,Liu JM,Zhao GJ,Wang L,Liang G,Wu B,Li MF,Qiu QM,Lu ZQ




Has Abstract


2013-11-01 00:00:00














  • Thromboxane synthase suppression induces lung cancer cell apoptosis via inhibiting NF-κB.

    abstract::Accumulating evidence shows that the inhibition of thromboxane synthase (TXS) induced apoptosis in cancer cells. TXS inhibitor 1-Benzylimidzole (1-BI) can trigger apoptosis in lung cancer cells but the mechanism is not fully defined. In this study, lung cancer cells were treated with 1-BI. In this study, the level of ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Leung KC,Li MY,Leung BC,Hsin MK,Mok TS,Underwood MJ,Chen GG

    更新日期:2010-12-10 00:00:00

  • BST-2/Tetherin is involved in BAFF-enhanced proliferation and survival via canonical NF-κB signaling in neoplastic B-lymphoid cells.

    abstract::The development of Sjögren's syndrome (SS) is accompanied by B cell hyperproliferation and mutation. Our previous study identified aberrant expression of BST-2 (also known as Tetherin/CD317) in B cells from either the peripheral blood or infiltrated salivary glands. However, the roles of BST-2 in the regulation of B c...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Fu J,Shi H,Zhan T,Li H,Ye L,Xie L,Wang Z,Wang B,Zheng L

    更新日期:2021-01-01 00:00:00

  • Role of calpain-9 and PKC-delta in the apoptotic mechanism of lumen formation in CEACAM1 transfected breast epithelial cells.

    abstract::CEACAM1-4S (carcinoembryonic antigen-related cell adhesion molecule 1) is a type I membrane protein with a short (12-amino acid) cytoplasmic tail. Wild type CEACAM1-4S-transfected MCF7 cells form glands with lumena when grown in 3D culture, while null mutations of two putative phosphorylation sites (T457A and S459A) i...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Chen CJ,Nguyen T,Shively JE

    更新日期:2010-02-15 00:00:00

  • Identification of the GTPase-activating protein DEP domain containing 1B (DEPDC1B) as a transcriptional target of Pitx2.

    abstract::Pitx2 is a bicoid-related homeobox transcription factor implicated in regulating left-right patterning and organogenesis. However, only a limited number of Pitx2 downstream target genes have been identified and characterized. Here we demonstrate that Pitx2 is a transcriptional repressor of DEP domain containing 1B (DE...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Wu D,Zhu X,Jimenez-Cowell K,Mold AJ,Sollecito CC,Lombana N,Jiao M,Wei Q

    更新日期:2015-04-10 00:00:00

  • Organization of early and late replicating DNA in human chromosome territories.

    abstract::It has been suggested that DNA organized into replication foci during S-phase remains stably aggregated in non-S-phase cells and that these stable aggregates provide fundamental units of nuclear or chromosome architecture [C. Meng and R. Berezney (1991) J. Cell Biol. 115, 95a; E. Sparvoli et al. (1994) J. Cell Sci. 10...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Zink D,Bornfleth H,Visser A,Cremer C,Cremer T

    更新日期:1999-02-25 00:00:00

  • Melanoma cells produce multiple laminin isoforms and strongly migrate on α5 laminin(s) via several integrin receptors.

    abstract::Melanoma cells express and interact with laminins (LMs) and other basement membrane components during invasion and metastasis. In the present study we have investigated the production and migration-promoting activity of laminin isoforms in melanoma. Immunohistochemistry of melanoma specimens and immunoprecipitation/we...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Oikawa Y,Hansson J,Sasaki T,Rousselle P,Domogatskaya A,Rodin S,Tryggvason K,Patarroyo M

    更新日期:2011-05-01 00:00:00

  • Macrophages enhance tumor-derived autophagosomes (DRibbles)-induced B cells activation by TLR4/MyD88 and CD40/CD40L.

    abstract::Our previous studies have showed that tumor-derived autophagosomes (termed "DRibbles") induce B cell activation, resulting in antibody production and cytokine secretion. Unexpectedly, we found that unfractionated splenocytes produced a higher level of antibody and cytokine than that of purified B cells. In the current...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Zhou M,Li W,Wen Z,Sheng Y,Ren H,Dong H,Cao M,Hu HM,Wang LX

    更新日期:2015-02-15 00:00:00

  • Liver 'organ on a chip'.

    abstract::The liver plays critical roles in both homeostasis and pathology. It is the major site of drug metabolism in the body and, as such, a common target for drug-induced toxicity and is susceptible to a wide range of diseases. In contrast to other solid organs, the liver possesses the unique ability to regenerate. The phys...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Beckwitt CH,Clark AM,Wheeler S,Taylor DL,Stolz DB,Griffith L,Wells A

    更新日期:2018-02-01 00:00:00

  • Production of trypsin by cells of the exocrine pancreas is paralleled by the expression of the KH protein vigilin.

    abstract::Vigilin, a protein with a continuous series of 14 KH motifs, forms part of a multiprotein complex containing tRNA. Several lines of evidence have suggested that vigilin expression is enhanced in those cells which were actively engaged in protein synthesis. Accordingly, we show here by immunoelectronmicroscopy a close ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Kruse C,Emmrich J,Rumpel E,Klinger MH,Grünweller A,Rohwedel J,Krammer HJ,Kühnel W,Müller PK

    更新日期:1998-02-25 00:00:00

  • Development of a chemically defined serum-free medium for differentiation of rat adipose precursor cells.

    abstract::Stromal-vascular cells from the epididymal fat pad of 4-week-old rats, when cultured in a medium containing insulin or insulin-like growth factor, IGF-I, triiodothyronine and transferrin, were able to undergo adipose conversion. Over ninety percent of the cells accumulated lipid droplets and this proportion was reduce...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Deslex S,Negrel R,Ailhaud G

    更新日期:1987-01-01 00:00:00

  • Isoforms of p38MAPK gamma and delta contribute to differentiation of human AML cells induced by 1,25-dihydroxyvitamin D₃.

    abstract::Inhibition of p38MAPK alpha/beta is known to enhance 1,25-dihydroxyvitamin (1,25D)-induced monocytic differentiation, but the detailed mechanism of this effect was not clear. We now show that the enhancement of differentiation becomes apparent with slow kinetics (12-24 h). Interestingly, the inhibition of p38MAPK alph...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Zhang J,Harrison JS,Studzinski GP

    更新日期:2011-01-01 00:00:00

  • Autophagy is involved in the differentiation of epicardial progenitor cells into vascular smooth muscle cells in mice.

    abstract::Most coronary smooth muscle cells(CoSMCs) are differentiated from epicardial progenitor cells(EPCs), but the specific mechanism is not fully investigated. Previous studies have shown that autophagy plays an important role for smooth muscle cells(SMCs) differentiation, yet whether autophagy is involved in the different...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Liu Y,Liu B,Li Y,Li Y,Du J,Deng S,Jing X,She Q

    更新日期:2019-02-15 00:00:00

  • Huntingtin interacting protein HYPK is a negative regulator of heat shock response and is downregulated in models of Huntington's Disease.

    abstract::Huntingtin interacting protein HYPK (Huntingtin Yeast Partner K) is an intrinsically unstructured protein having chaperone-like activity and can suppress mutant huntingtin aggregates and toxicity in cell model of Huntington's Disease (HD). Heat shock response is an adaptive mechanism of cells characterized by upregula...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Das S,Bhattacharyya NP

    更新日期:2016-05-01 00:00:00

  • Tumor-specific induction of apoptosis by a p53-reactivating compound.

    abstract::The tumor suppressor function of p53 is disabled in the majority of tumors, either by a point mutation of the p53 gene, or via MDM2-dependent proteasomal degradation. We have screened a chemical library using a cell-based assay and identified a low molecular weight compound named MITA which induced wild-type p53-depen...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Hedström E,Issaeva N,Enge M,Selivanova G

    更新日期:2009-02-01 00:00:00

  • Elevated levels of polyamines alter chromatin in murine skin and tumors without global changes in nucleosome acetylation.

    abstract::Polyamines affect nucleosome oligomerization and DNA conformation in vitro, yet little information exists regarding the influence of naturally synthesized polyamines on mammalian chromatin. Capitalizing on the relative inefficiency of a moderate ionic strength extraction buffer to dissociate histones, we obtained evid...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Hobbs CA,Paul BA,Gilmour SK

    更新日期:2003-11-01 00:00:00

  • Kidins220/ARMS depletion is associated with the neural-to Schwann-like transition in a human neuroblastoma cell line model.

    abstract::Peripheral neuroblastic tumors exist as a heterogeneous mixture of neuroblastic (N-type) cells and Schwannian stromal (S-type) cells. These stromal cells not only represent a differentiated and less aggressive fraction of the tumor, but also have properties that can influence the further differentiation of nearby mali...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Rogers DA,Schor NF

    更新日期:2013-03-10 00:00:00

  • Overexpression of soluble ADAM33 promotes a hypercontractile phenotype of the airway smooth muscle cell in rat.

    abstract::A disintegrin and metalloproteinase 33 (ADAM33) has been identified as a susceptibility gene for asthma, but details of the causality are not fully understood. We hypothesize that soluble ADAM33 (sADAM33) overexpression can alter the mechanical behaviors of airway smooth muscle cells (ASMCs) via regulation of the cell...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Duan Y,Long J,Chen J,Jiang X,Zhu J,Jin Y,Lin F,Zhong J,Xu R,Mao L,Deng L

    更新日期:2016-11-15 00:00:00

  • Altered sensitivity to retinoid-induced apoptosis associated with changes in the subcellular distribution of Bcl-2.

    abstract::In the acute promyelocytic leukemia cell line NB4, Bcl-2 downregulation occurred as a late event of retinoid-induced differentiation. In the maturation-resistant NB4-R1 subclone, retinoids failed to downregulate Bcl-2 even in the situation of apoptosis massively induced by pan-agonists and RXR-selective agonists. We o...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Bruel A,Karsenty E,Schmid M,McDonnell TJ,Lanotte M

    更新日期:1997-06-15 00:00:00

  • Intranuclear distribution of SV40 large T-antigen and transformation-related protein p53 in abortively infected cells.

    abstract::The intranuclear localization of SV40 T-antigen (T-Ag) and the cellular protein p53 was studied in SV40 abortively infected baby mouse kidney cells using two complementary methods of ultrastructural immunocytochemistry in combination with preferential staining of nuclear RNP components and electron microscope autoradi...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Puvion E,Duthu A,Harper F,Ehrhart JC,Viron A,May P

    更新日期:1988-07-01 00:00:00

  • Alpha-factor inhibition of the rate of cell passage through the "start" step of cell division in Saccharomyces cerevisiae yeast: estimation of the division delay per alpha-factor.receptor complex.

    abstract::A highly sensitive, kinetically unambiguous assay for alpha-factor-induced delay of cell passage through the "start" step of cell division in yeast is presented. The assay employs perfusion with periodic microscopy to monitor the bud emergence kinetics on the 20% of cells within an exponentially growing population whi...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Moore SA

    更新日期:1987-08-01 00:00:00

  • Expression of proliferating cell nuclear antigen in migrating retinal pigment epithelial cells during wound healing in organ culture.

    abstract::Although 12-day-old chick embryo retinal pigment epithelial (RPE) cells in situ do not express the proliferating cell nuclear antigen (PCNA) which is known to function as an auxiliary protein of DNA polymerase delta, they do so when cultured on glass. Conversely, PCNA was not expressed by RPE cells of the same age mai...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Hergott GJ,Kalnins VI

    更新日期:1991-08-01 00:00:00

  • The coxsackie- and adenovirus receptor (CAR) is an in vivo marker for epithelial tight junctions, with a potential role in regulating permeability and tissue homeostasis.

    abstract::The coxsackie- and adenovirus receptor (CAR) is a transmembrane protein belonging to the immunoglobulin superfamily. The function of CAR as a virus receptor has been extensively analyzed, while its physiological role and expression pattern in adult tissues have remained less clear. CAR associates with epithelial tight...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Raschperger E,Thyberg J,Pettersson S,Philipson L,Fuxe J,Pettersson RF

    更新日期:2006-05-15 00:00:00

  • Roles of bHLH genes in neural stem cell differentiation.

    abstract::Neural stem cells change their characteristics over time during development: they initially proliferate only and then give rise to neurons first and glial cells later. In the absence of the repressor-type basic helix-loop-helix (bHLH) genes Hes1, Hes3 and Hes5, neural stem cells do not proliferate sufficiently but pre...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Kageyama R,Ohtsuka T,Hatakeyama J,Ohsawa R

    更新日期:2005-06-10 00:00:00

  • Caloric restriction: conservation of cellular replicative capacity in vitro accompanies life-span extension in mice.

    abstract::We have tested whether life-long caloric restriction (CR) slows or delays the age-related loss of cellular replicative potential that occurs during normal aging in ad libitum (AL) fed mice. Both mean and maximum life spans of the restricted animals (60% of AL intake) were significantly extended 30-40% by CR treatment....

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Pendergrass WR,Li Y,Jiang D,Fei RG,Wolf NS

    更新日期:1995-04-01 00:00:00

  • Reactivation of an inactive human X chromosome introduced into mouse embryonal carcinoma cells by microcell fusion with persistent expression of XIST.

    abstract::An inactive human X chromosome was introduced by microcell fusion into two mouse embryonal carcinoma cell lines, PSA1-TG8 and OTF9-63, each of which has a single X chromosome. The donor cell line was a mouse-human somatic cell hybrid, CF150, retaining one or more inactive human X chromosome(s) per cell as its only hum...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Yoshida I,Nishita Y,Mohandas TK,Takagi N

    更新日期:1997-02-01 00:00:00

  • SIX4 activates Akt and promotes tumor angiogenesis.

    abstract::Angiogenesis plays important roles in solid tumors progression. Growth factors such as vascular endothelial growth factors (VEGFs) can induce angiogenesis and hypoxia promotes the expression of VEGFs through activating hypoxia-inducible factor 1 (HIF-1α). However, the regulation of HIF-1α still not been fully understo...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Sun X,Hu F,Hou Z,Chen Q,Lan J,Luo X,Wang G,Hu J,Cao Z

    更新日期:2019-10-01 00:00:00

  • Downregulated lncRNA-SNHG1 enhances autophagy and prevents cell death through the miR-221/222 /p27/mTOR pathway in Parkinson's disease.

    abstract::Autophagy has been shown to be critically associated with the central mechanisms underlying Parkinson's disease (PD), while the mechanisms contributing to the imbalance of autophagy remain unclear. Small nucleolar RNA host gene 1 (SNHG1), a well-studied long noncoding RNA, has been reported to be significantly increas...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Qian C,Ye Y,Mao H,Yao L,Sun X,Wang B,Zhang H,Xie L,Zhang H,Zhang Y,Zhang S,He X

    更新日期:2019-11-01 00:00:00

  • Cytofluorometric DNA base determination for the investigation of heterochromatin and heterochromatin amplification.

    abstract::The measurement of chromomycin A3/DAPI fluorescence ratios is shown to allow base content determinations in eu- and heterochromatic regions of interphase cell nuclei. The base content values obtained in chromocenters and euchromatin of Scilla sibirica agree with those measured earlier [12] on the band and non-band are...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Leemann U,Ruch F

    更新日期:1983-09-01 00:00:00

  • Downregulation of endogenous STAT3 augments tumoricidal activity of interleukin 15 activated dendritic cell against lymphoma and leukemia via TRAIL.

    abstract::Effector functions in tumor resistance by dendritic cells (DCs) are less well characterized. In this study, we describe that the murine DCs upon stimulation with recombinant IL-15 in vitro or in vivo, expresses TNF superfamily member TRAIL which mediates cytotoxicity and growth inhibition against a murine lymphoma cal...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Hira SK,Mondal I,Bhattacharya D,Manna PP

    更新日期:2014-10-01 00:00:00

  • The role of autophagy in THP-1 macrophages resistance to HIV- vpr-induced apoptosis.

    abstract::Macrophages are resistant to cell death and are one of HIV reservoirs. HIV viral protein Vpr has the potential to promote infection of and survival of macrophages, which could be a highly significant factor in the development and/or maintenance of macrophage viral reservoirs. However, the impact of vpr on macrophages ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Zhou HY,Zheng YH,He Y,Chen Z,He B

    更新日期:2017-02-01 00:00:00