5-azacytidine reactivates the erythroid differentiation potential of the myeloid-restricted murine cell line 32D Ro.

Abstract:

:32D cells grown for 1 year in interleukin-3 (IL-3) and granulocyte colony-stimulating factor (G-CSF) generated the 32D Ro cell line which retained the parental mast cell phenotype but lost ability to generate erythroid cells in response to erythropoietin (EPO). In order to clarify the mechanisms underlying such restriction, we compared 32D and 32D Ro cells for their capacity to express erythroid-specific transcription factors (Gata1, Gata2, Scl, Nef2, Eklf, and Id) and the capacity of short exposure to 5-azacytidine (5-AzaC) to reactivate erythroid differentiation potential in 32D Ro cells. By Northern analysis, the two cell lines expressed similar levels of all these genes. However, after being treated with 5-AzaC, 32D Ro cells acquired the ability to generate EPO-dependent clones (1 clone/10(4) cells) which gave rise to EPO-dependent cell lines. All the 10 EPO-responsive cell lines independently isolated from 5-AzaC-treated 32D Ro cells had erythroid morphology and expressed high levels of alpha- and beta-globin. In contrast, none of the IL-3-dependent and granulocyte/macrophage colony-stimulating factor-dependent clones concurrently isolated, as a control, showed erythroid properties. Therefore, 5-AzaC treatment reactivates the potential of the myeloid-restricted 32D Ro cells to generate EPO-responsive erythroid clones suggesting that gene methylation played an important role in the G-CSF-mediated restriction/activation of the differentiation potential of these cells.

journal_name

Exp Cell Res

authors

Baiocchi M,Di Rico C,Di Pietro R,Di Baldassarre A,Migliaccio AR

doi

10.1016/s0014-4827(03)00032-6

keywords:

subject

Has Abstract

pub_date

2003-05-01 00:00:00

pages

258-67

issue

2

eissn

0014-4827

issn

1090-2422

pii

S0014482703000326

journal_volume

285

pub_type

杂志文章
  • Expression of the first N-glycosylation gene in the dolichol pathway, ALG7, is regulated at two major control points in the G1 phase of the Saccharomyces cerevisiae cell cycle.

    abstract::The Saccharomyces cerevisiae ALG7 gene, which functions by initiating the dolichol pathway of protein N-glycosylation, displays properties of an early growth-response gene. To initiate studies of the involvement of ALG7 in cellular proliferation, we have now more precisely analyzed ALG7 expression in the G1 phase of c...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1995.1255

    authors: Pretel R,Lennon K,Bird A,Kukuruzinska MA

    更新日期:1995-08-01 00:00:00

  • Lipopolysaccharide-induced activation of NF-κB non-canonical pathway requires BCL10 serine 138 and NIK phosphorylations.

    abstract:BACKGROUND AND AIMS:B-cell lymphoma/leukemia (BCL)-10 and reactive oxygen species mediate two pathways of NF-κB (RelA) activation by lipopolysaccharide (LPS) in human colonic epithelial cells. The pathway for LPS activation of RelB by the non-canonical pathway (RelB) in non-myeloid cells was not yet reported, but impor...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2010.05.004

    authors: Bhattacharyya S,Borthakur A,Dudeja PK,Tobacman JK

    更新日期:2010-11-15 00:00:00

  • Intracellular distribution of poly(ADP-ribose) synthetase in rat spermatogenic cells.

    abstract::The highest activity of poly(ADP-ribose) synthetase was found in the testis among several rat tissues tested. Subcellular fractionation of the testis demonstrated that the synthetase was localized primarily in the nucleus and partially in the microsomal-ribosomal fraction. This result was confirmed by immunocytochemic...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(89)90063-3

    authors: Concha II,Figueroa J,Concha MI,Ueda K,Burzio LO

    更新日期:1989-02-01 00:00:00

  • Turbidimetric studies on microtubule sliding using the stopped-flow-light-scattering method.

    abstract::The turbidity of axonemes during active sliding of microtubules was analysed using the stopped-flow-light-scattering method with high time resolution. Flagella of sea-urchin spermatozoa were demembranated and used after a brief treatment with trypsin. The turbidity of the suspension of flagellar axonemes during ATP-in...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(86)90571-9

    authors: Kamimura S,Nakanishi M,Yano M,Shimizu H

    更新日期:1986-03-01 00:00:00

  • Internalization of the hyaluronan receptor CD44 by chondrocytes.

    abstract::Chondrocytes express CD44 as a primary receptor for the matrix macromolecule hyaluronan. Hyaluronan is responsible for the retention and organization of proteoglycan within cartilage, and hyaluronan-chondrocyte interactions are important for the assembly and maintenance of the cartilage matrix. Bovine articular chondr...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1999.4641

    authors: Aguiar DJ,Knudson W,Knudson CB

    更新日期:1999-11-01 00:00:00

  • Possible role of deep tubular invaginations of the plasma membrane in MHC-I trafficking.

    abstract::Tubules and vesicles are membrane carriers involved in traffic along the endocytic and secretory routes. The small GTPase Arf6 regulates a recycling branch of short dynamic tubular intermediates used by major histocompatibility class I (MHC-I) molecules to traffic through vesicles between endosomes and the plasma memb...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2005.01.021

    authors: Massol RH,Larsen JE,Kirchhausen T

    更新日期:2005-05-15 00:00:00

  • Novel approach to abuse the hyperactive K-Ras pathway for adenoviral gene therapy of colorectal cancer.

    abstract:BACKGROUND:Functional activation of oncogenic K-Ras signaling pathway plays an important role in the early events of colorectal carcinogenesis (CRC). K-Ras proto-oncogene is involved in 35-40% of CRC cases. Mutations in the Ras gene trigger the transduction of proliferative and anti-apoptotic signals, even in the absen...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2011.09.015

    authors: Naumov I,Kazanov D,Lisiansky V,Starr A,Aroch I,Shapira S,Kraus S,Arber N

    更新日期:2012-01-15 00:00:00

  • Inhibition of tumor growth by a truncated and soluble form of melanotransferrin.

    abstract::Melanotransferrin is a glycoprotein expressed at the cell membrane and secreted in the extracellular environment. Recombinant truncated form of membrane-bound melanotransferrin (sMTf) was reported to exert in vitro anti-angiogenic properties. Here we show that sMTf treatment leads to a 50% inhibition of neovasculariza...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2007.04.013

    authors: Rolland Y,Demeule M,Michaud-Levesque J,Béliveau R

    更新日期:2007-08-01 00:00:00

  • Promotion of skeletal muscle differentiation by K252a with tyrosine phosphorylation of focal adhesion: a possible involvement of small GTPase Rho.

    abstract::K252a, a protein kinase inhibitor, acts as a neurotrophic factor in several neuronal cells. In this study we show that K252a enhanced the differentiation of C2C12 myoblasts as well as tyrosine phosphorylation of several focal adhesion-associated proteins including p130(Cas), focal adhesion kinase, and paxillin. The ty...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1999.4648

    authors: Lee KH,Lee SH,Kim D,Rhee S,Kim C,Chung CH,Kwon H,Kang MS

    更新日期:1999-11-01 00:00:00

  • Selective photothermal efficiency of citrate capped gold nanoparticles for destruction of cancer cells.

    abstract::Gold nanoparticles are recently having much attention because of their increased applications in biomedical fields. In this paper, we demonstrated the photothermal efficacy of citrate capped gold nanoparticles (AuNPs) for the destruction of A431 cancer cells. Citrate capped AuNPs were synthesized successfully and char...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2011.04.010

    authors: Raji V,Kumar J,Rejiya CS,Vibin M,Shenoi VN,Abraham A

    更新日期:2011-08-15 00:00:00

  • Stability and nuclear distribution of mammalian replication protein A heterotrimeric complex.

    abstract::Replication protein A (RPA), a stable complex of three polypeptides, is the single-stranded DNA-binding protein essential for DNA replication in eukaryotic cells. Previous studies of the subcellular distribution and stability of the RPA heterotrimer during the mammalian cell cycle have produced conflicting results. He...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1999.4770

    authors: Dimitrova DS,Gilbert DM

    更新日期:2000-02-01 00:00:00

  • An in vitro tubule assay identifies HGF as a morphogen for the formation of seminiferous tubules in the postnatal mouse testis.

    abstract::We have been working with a recently immortalized Sertoli cell line, SF7, that appears to produce sleeves, or hollow tubules, when cultivated on a layer of growth factor-reduced Matrigel (GFR-Matrigel) in medium supplemented with serum. We tried to determine which components of GFR-Matrigel and serum provide the envir...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1999.4630

    authors: van der Wee K,Hofmann MC

    更新日期:1999-10-10 00:00:00

  • DEPDC1B knockdown inhibits the development of malignant melanoma through suppressing cell proliferation and inducing cell apoptosis.

    abstract::Malignant melanoma (MM) remains the leading cause of skin cancer related death, which has very poor prognosis because of locoregional recurrence and distant metastasis. DEPDC1B (DEP domain-containing protein 1B), has been proved to be associated with some types of malignant tumors. However, the role of DEPDC1B in MM i...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2019.03.021

    authors: Xu Y,Sun W,Zheng B,Liu X,Luo Z,Kong Y,Xu M,Chen Y

    更新日期:2019-06-01 00:00:00

  • Epigenetic modifications and chromatin loop organization explain the different expression profiles of the Tbrg4, WAP and Ramp3 genes.

    abstract::Whey Acidic Protein (WAP) gene expression is specific to the mammary gland and regulated by lactogenic hormones to peak during lactation. It differs markedly from the more constitutive expression of the two flanking genes, Ramp3 and Tbrg4. Our results show that the tight regulation of WAP gene expression parallels var...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2008.01.001

    authors: Montazer-Torbati MB,Hue-Beauvais C,Droineau S,Ballester M,Coant N,Aujean E,Petitbarat M,Rijnkels M,Devinoy E

    更新日期:2008-03-10 00:00:00

  • SIRT6 inhibits TNF-α-induced inflammation of vascular adventitial fibroblasts through ROS and Akt signaling pathway.

    abstract::SIRT6, with both deacetylase and ADP-ribosyltransferase activities, is predominantly expressed in the nucleus. It has been revealed that SIRT6 regulates various biological functions including metabolism, aging and stress resistance. This study aims to investigate the role of SIRT6 in vascular inflammation and it molec...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2017.05.001

    authors: He Y,Xiao Y,Yang X,Li Y,Wang B,Yao F,Shang C,Jin Z,Wang W,Lin R

    更新日期:2017-08-01 00:00:00

  • Doubling of cell mass is not necessary in order to achieve cell division in cultured human cells.

    abstract::When exponentially growing NHIK 3025 cells were shifted from medium containing 30% serum to medium containing 0.03% serum the rate of net protein accumulation was reduced due to both a reduction in the rate of protein synthesis and an increase in the rate of protein degradation. This change in growth conditions increa...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(84)90788-2

    authors: Rønning OW,Pettersen EO

    更新日期:1984-11-01 00:00:00

  • External Ca2+ regulates polycystin-2 (TRPP2) cation currents in LLC-PK1 renal epithelial cells.

    abstract::Polycystin-2 (PC2, TRPP2) is a nonselective cation channel whose dysfunction is associated with the onset of autosomal dominant polycystic kidney disease (ADPKD). PC2 contributes to Ca2+ transport and cell signaling in renal epithelia and other tissues. Little is known however, as to the external Ca2+ regulation of PC...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2016.11.004

    authors: Dai XQ,Perez PL,Soria G,Scarinci N,Smoler M,Morsucci DC,Suzuki K,Cantero MDR,Cantiello HF

    更新日期:2017-01-01 00:00:00

  • Differential sensitivity of metaphase to diamide and ouabain in HeLa cells.

    abstract::We have examined the effects of diamide, an oxidizer of glutathione, on the progress of HeLa cells through the cell cycle. At concentrations which do not significantly alter generation time, anaphase or cytokinesis, diamide causes a two-fold increase in the duration of metaphase. At 3 X 10(-8) M, ouabain also prolongs...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(85)90449-5

    authors: Ziegler ML,Sisken JE

    更新日期:1985-05-01 00:00:00

  • Comparative epigenetic influence of autologous versus fetal bovine serum on mesenchymal stem cells through in vitro osteogenic and adipogenic differentiation.

    abstract::Mesenchymal stem cells (MSCs) derived from bone marrow (BM) represents a useful source of adult stem cells for cell therapy and tissue engineering. MSCs are present at a low frequency in the BM; therefore expansion is necessary before performing clinical studies. Fetal bovine serum (FBS) as a nutritional supplement fo...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2015.10.009

    authors: Fani N,Ziadlou R,Shahhoseini M,Baghaban Eslaminejad M

    更新日期:2016-06-10 00:00:00

  • A composite CMV-IE enhancer/beta-actin promoter is ubiquitously expressed in mouse cutaneous epithelium.

    abstract::In most existing transgenic mouse models developed for the study of specific genes in the skin, the goal has been to target transgene expression to defined populations of cells in the cutaneous epithelium. Keratin promoters have been especially useful for this purpose. In some instances, however, it may be desirable t...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1998.4175

    authors: Sawicki JA,Morris RJ,Monks B,Sakai K,Miyazaki J

    更新日期:1998-10-10 00:00:00

  • Coagulation factor Xa drives tumor cells into apoptosis through BH3-only protein Bim up-regulation.

    abstract::Coagulation Factor (F)Xa is a serine protease that plays a crucial role during blood coagulation by converting prothrombin into active thrombin. Recently, however, it emerged that besides this role in coagulation, FXa induces intracellular signaling leading to different cellular effects. Here, we show that coagulation...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2007.04.014

    authors: Borensztajn KS,Bijlsma MF,Groot AP,Brüggemann LW,Versteeg HH,Reitsma PH,Peppelenbosch MP,Spek CA

    更新日期:2007-07-15 00:00:00

  • Functional conservation of 14-3-3 isoforms in inhibiting bad-induced apoptosis.

    abstract::14-3-3 proteins are a family of homologous eukaryotic molecules with seven distinct isoforms in mammalian cells. Isoforms of 14-3-3 proteins interact with diverse ligands and are involved in the regulation of mitogenesis, cell cycle progression, and apoptosis. However, whether different 14-3-3 isoforms are responsible...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.2001.5376

    authors: Subramanian RR,Masters SC,Zhang H,Fu H

    更新日期:2001-11-15 00:00:00

  • Development of a chemically defined serum-free medium for differentiation of rat adipose precursor cells.

    abstract::Stromal-vascular cells from the epididymal fat pad of 4-week-old rats, when cultured in a medium containing insulin or insulin-like growth factor, IGF-I, triiodothyronine and transferrin, were able to undergo adipose conversion. Over ninety percent of the cells accumulated lipid droplets and this proportion was reduce...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(87)90412-5

    authors: Deslex S,Negrel R,Ailhaud G

    更新日期:1987-01-01 00:00:00

  • The RGD integrin binding site in human L1-CAM is important for nuclear signaling.

    abstract::L1 cell adhesion molecule (L1-CAM) is a transmembrane cell adhesion molecule initially defined as a promigratory molecule in the developing nervous system. L1 is also overexpressed in a variety of human carcinomas and is associated with bad prognosis. In carcinoma cell lines L1 augments cell motility and metastasis, t...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2008.04.004

    authors: Gast D,Riedle S,Kiefel H,Müerköster SS,Schäfer H,Schäfer MK,Altevogt P

    更新日期:2008-08-01 00:00:00

  • Shp-2 tyrosine phosphatase: signaling one cell or many.

    abstract::Shp-2, a widely expressed cytoplasmic tyrosine phosphatase with two src-homology 2 (SH2) domains, has received much attention in the signal transduction field recently. Significant progress has been made in understanding the structure and function of this phosphatase, together with its Drosophila homologue, Corkscrew,...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审

    doi:10.1006/excr.1999.4668

    authors: Feng GS

    更新日期:1999-11-25 00:00:00

  • GSK-3beta inhibition by lithium confers resistance to chemotherapy-induced apoptosis through the repression of CD95 (Fas/APO-1) expression.

    abstract::Lithium exerts neuroprotective actions that involve the inhibition of glycogen synthase kinase-3beta (GSK-3beta). Otherwise, recent studies suggest that sustained GSK-3beta inhibition is a hallmark of tumorigenesis. In this context, the present study was undertaken to examine whether lithium modulated cancer cell sens...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2004.08.001

    authors: Beurel E,Kornprobst M,Blivet-Van Eggelpoël MJ,Ruiz-Ruiz C,Cadoret A,Capeau J,Desbois-Mouthon C

    更新日期:2004-11-01 00:00:00

  • Loss of Dnd1 facilitates the cultivation of genital ridge-derived rat embryonic germ cells.

    abstract::Pluripotent cells referred to as embryonic germ cells (EGCs) can be derived from the embryonic precursors of the mature gametes: the primordial germ cells (PGCs). A homozygous mutation (ter) of the dead-end homolog 1 gene (Dnd1) in the rat causes gonadal teratocarcinogenesis and sterility due to neoplastic transformat...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2011.04.013

    authors: Northrup E,Eisenblätter R,Glage S,Rudolph C,Dorsch M,Schlegelberger B,Hedrich HJ,Zschemisch NH

    更新日期:2011-08-01 00:00:00

  • Reserpine-induced alterations in mucus production and calmodulin-binding proteins in a human epithelial cell line.

    abstract::The characterization of a mucus-producing human cell line (HC-84) derived from a colon carcinoma and its response to in vitro reserpine treatment is reported. Mucous granules were demonstrated within these cells on the basis of electron microscopic examination and incorporation of [3H]glucosamine with subsequent autor...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(84)90709-2

    authors: Brady RC,Karnaky KJ Jr,Dedman JR

    更新日期:1984-01-01 00:00:00

  • Human dermal fibroblasts escape from the long-term phenocopy of senescence induced by psoralen photoactivation.

    abstract::We have previously shown that following psoralen photoactivation (PUVA treatment) human dermal fibroblasts undergo long-term growth arrest as well as morphological and functional changes reminiscent of cellular senescence [ 1 ]. In the absence of molecular data on what constitutes normal senescence, it has been diffic...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.2002.5476

    authors: Ma W,Wlaschek M,Brenneisen P,Schneider LA,Hommel C,Hellweg C,Sauer H,Wartenberg M,Herrmann G,Meewes C,Boukamp P,Scharffetter-Kochanek K

    更新日期:2002-04-01 00:00:00

  • A-type nuclear lamins act as transcriptional repressors when targeted to promoters.

    abstract::Regions of heterochromatin are often found at the periphery of the mammalian nucleus, juxtaposed to the nuclear lamina. Genes in these regions are likely maintained in a transcriptionally silent state, although other locations at the nuclear periphery associated with nuclear pores are sites of active transcription. As...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2009.01.003

    authors: Lee DC,Welton KL,Smith ED,Kennedy BK

    更新日期:2009-04-01 00:00:00