Domain analysis of the actin-binding and actin-remodeling activities of drebrin.

abstract：:Germ cells were isolated from mouse fetal gonads 11 1/2-16 1/2 days post coitum (dpc), and exposed to various methods of in vitro culture. From 13 1/2 dpc onwards, both male and female germ cells survived well at 37 degrees C for several days. During the culture period the proportion of female germ cells in meiosis in...

journal_title：Experimental cell research

authors： De Felici M,McLaren A

更新日期：1983-04-01 00:00:00

abstract：:The bone morphogenetic proteins (BMPs) are potent osteoinductive factors that accelerate osteoblast maturation, accompanied by increased cell-substrate adhesion. BMP-2 treatment of osteoblastic cells increases phosphorylation of the cytoplasmic BMP-2 signaling molecules, Smad1 and Smad5. We have previously reported th...

journal_title：Experimental cell research

authors： Nöth U,Tuli R,Seghatoleslami R,Howard M,Shah A,Hall DJ,Hickok NJ,Tuan RS

更新日期：2003-11-15 00:00:00

abstract：:Epidermal growth factor (EGF) and transforming growth factor-beta1 (TGF-beta1), upregulated in renal diseases, have a combinational effect on epithelial-mesenchymal transformation (EMT) of renal proximal tubular cells. The aim of this study was to examine the mechanism regarding the combinational effect of EGF and TGF...

journal_title：Experimental cell research

authors： Tian YC,Chen YC,Chang CT,Hung CC,Wu MS,Phillips A,Yang CW

更新日期：2007-07-01 00:00:00

abstract：:Pancreatic cancer is characterized by aggressive local invasion and early metastasis formation. Active migration of the pancreatic cancer cells is essential for these processes. We have shown previously that the pancreatic cancer cells lines CFPAC1 and IMIM-PC2 show high migratory activity, and we have investigated he...

journal_title：Experimental cell research

authors： Stock AM,Hahn SA,Troost G,Niggemann B,Zänker KS,Entschladen F

更新日期：2014-08-15 00:00:00

abstract：:We have reported that PAP-1, a product of a causative gene for autosomal retinitis pigmentosa, plays a role in splicing. In this study, CIR, a protein originally identified as a CBF1-interacting protein and reported to act as a transcriptional corepressor, was identified as a PAP-1 binding protein and its function as ...

journal_title：Experimental cell research

authors： Maita H,Kitaura H,Ariga H,Iguchi-Ariga SM

更新日期：2005-02-15 00:00:00

abstract：:There is increasing evidence that the hypersensitive response during plant-pathogen interactions is a form of programmed cell death. In an attempt to understand the biochemical nature of this form of programmed cell death in the cowpea-cowpea rust fungus system, proteolytic activity in extracts of fungus-infected and ...

journal_title：Experimental cell research

authors： D'Silva I,Poirier GG,Heath MC

更新日期：1998-12-15 00:00:00

abstract：:The migration and proliferation of vascular smooth muscle cells (vSMCs) are critical events in neointima formation during atherosclerosis and restenosis. The transcription factor nuclear factor of activated T-cells-isoform c1 (NFATc1) is regulated by atherogenic cytokines, and has been implicated in the migratory and ...

journal_title：Experimental cell research

authors： Chow W,Hou G,Bendeck MP

更新日期：2008-10-01 00:00:00

abstract：:In multiple myeloma, which commonly depends on interleukin 6, IL-6, survival signaling induced by this cytokine is largely mediated by activation of STAT3. Interferon alpha (IFNalpha) treatment of cell lines derived from multiple myeloma or of myeloma tumor cells ex vivo leads to apoptosis. In this study we demonstrat...

journal_title：Experimental cell research

authors： Thyrell L,Arulampalam V,Hjortsberg L,Farnebo M,Grandér D,Pokrovskaja Tamm K

更新日期：2007-11-15 00:00:00

abstract：:Leptomycin B (LMB) is a Streptomyces metabolite that inhibits nuclear export of the human immunodeficiency virus type 1 regulatory protein Rev at low nanomolar concentrations. Recently, LMB was shown to inhibit the function of CRM1, a receptor for the nuclear export signal (NES). Here we show evidence that LMB binds d...

journal_title：Experimental cell research

authors： Kudo N,Wolff B,Sekimoto T,Schreiner EP,Yoneda Y,Yanagida M,Horinouchi S,Yoshida M

更新日期：1998-08-01 00:00:00

abstract：:We studied the commitment of 3T3-F442A cells during stimulation with adipogenic serum or growth hormone. Confluent 3T3-F442A preadipocytes were incubated with adipogenic medium for increasing times; the number of adipose clusters, GPDH activity, and lipid accumulation were evaluated. Results show that cell commitment ...

journal_title：Experimental cell research

authors： Castro-Muñozledo F,Beltrán-Langarica A,Kuri-Harcuch W

更新日期：2003-04-01 00:00:00

abstract：:Quinolinic acid (QUIN) is a glutamate agonist which markedly enhances the vulnerability of neural cells to excitotoxicity. QUIN is produced from the amino acid tryptophan through the kynurenine pathway (KP). Dysregulation of this pathway is associated with neurodegenerative conditions. In this study we treated striata...

journal_title：Experimental cell research

authors： Pierozan P,Ferreira F,Ortiz de Lima B,Gonçalves Fernandes C,Totarelli Monteforte P,de Castro Medaglia N,Bincoletto C,Soubhi Smaili S,Pessoa-Pureur R

更新日期：2014-04-01 00:00:00

abstract：:Mammalian stanniocalcin-2 (STC2) is a secreted glycoprotein hormone with a putative role in unfolded protein response and apoptosis. Here we reported that STC2 expression was sporadically abrogated in human cancer cells by transcriptional silencing associated with CpG island promoter hypermethylation. Direct sequencin...

journal_title：Experimental cell research

authors： Law AY,Lai KP,Ip CK,Wong AS,Wagner GF,Wong CK

更新日期：2008-05-01 00:00:00

abstract：:Culture and UV (254 nm) irradiation conditions that are suggested as appropriate for a study of the effect of UV on the limited in vitro lifespan of a normal human diploid fibroblast (HDF) strain are first described. An inoculation density at each subcultivation of 1.8 x 10(4) viable cells/cm-2 permits the decline in ...

journal_title：Experimental cell research

authors： Kantor GJ,Mulkie JR,Hull DR

更新日期：1978-05-01 00:00:00

abstract：:Renal expression of the aldolase B isoenzyme and transcription factors previously shown to regulate the aldolase B gene promoter in the liver were analyzed in whole kidney, microdissected tubules, and the two PKSV-PCT and PKSV-PR proximal tubule cell lines derived from transgenic mice. Aldolase B gene expression appea...

journal_title：Experimental cell research

authors： Vallet V,Bens M,Antoine B,Levrat F,Miquerol L,Kahn A,Vandewalle A

更新日期：1995-02-01 00:00:00

abstract：:Cells with and without hypoxanthine-guanine phosphoribosyltransferase (HGPRT) activity were used to examine the transfer of purine metabolites through the medium and via cell contacts. HGPRT- Chinese hamster and human fibroblasts were able to incorporate 3H-labeled purine metabolite(s) from medium in which mouse HGPRT...

journal_title：Experimental cell research

authors： Bols NC,Mosser DD,Boliska SA

更新日期：1987-11-01 00:00:00

abstract：:Cell-to-cell communication is essential for the development and maintenance of multicellular organisms. The tunneling nanotube (TNT) is a recently recognized distinct type of intercellular communication device. TNTs are thin protrusions of the plasma membrane and allow direct physical connections of the plasma membran...

journal_title：Experimental cell research

authors： Kimura S,Hase K,Ohno H

更新日期：2012-08-15 00:00:00

abstract：:Insulin-like growth factor binding proteins (IGFBPs) have various IGF-independent cellular activities, including receptor-independent cellular uptake followed by transcriptional regulation, although mechanisms of cellular entry remain unclear. Herein, we focused on their receptor-independent cellular entry mechanism i...

journal_title：Experimental cell research

authors： Goda N,Tenno T,Inomata K,Shirakawa M,Tanaka T,Hiroaki H

更新日期：2008-08-01 00:00:00

abstract：:Most cases of familial early-onset Alzheimer's disease are caused by mutations in the presenilin 1 (PS1) gene. However, the cellular functions of PS1 are unknown. We showed predominant localization of PS1 to cell-cell contacts of the plasma membrane in human prostate epithelial tissue and in a human epithelial cell li...

journal_title：Experimental cell research

authors： Singh N,Talalayeva Y,Tsiper M,Romanov V,Dranovsky A,Colflesh D,Rudamen G,Vitek MP,Shen J,Yang X,Goldgaber D,Schwarzman AL

更新日期：2001-02-01 00:00:00

abstract：:Mutations in the tumor suppressor gene p53 were found in more than 90% of all human squamous cell carcinomas (SCC). To study the function of p53 in a keratinocyte background, a tetracycline-controlled p53 transgene was introduced into a human SCC cell line (SCC15), lacking endogenous p53. Conditional expression of wil...

journal_title：Experimental cell research

authors： Niemantsverdriet M,Jongmans W,Backendorf C

更新日期：2005-10-15 00:00:00

abstract：:The glycolytic enzyme aldolase is concentrated in a domain around stress fibers in living Swiss 3T3 cells, but the mechanism by which aldolase is localized has not been revealed. We have recently identified a molecular binding site for F-actin on aldolase, and we hypothesized that this specific binding interaction, ra...

journal_title：Experimental cell research

authors： Wang J,Tolan DR,Pagliaro L

更新日期：1997-12-15 00:00:00

abstract：:Several metabolic enzymes assemble into distinct intracellular structures in prokaryotes and eukaryotes suggesting an important functional role in cell physiology. The CTP-generating enzyme CTP synthase forms long filamentous structures termed cytoophidia in bacteria, yeast, fruit flies and human cells independent of ...

journal_title：Experimental cell research

authors： Huang Y,Wang JJ,Ghosh S,Liu JL

更新日期：2017-05-15 00:00:00

abstract：:We studied the guidance and activation of macrophages from the P388D1 cell line and rat peritoneum by topographic features on a nanometric scale. Cells were plated on plain fused silica substrata or substrata with microfabricated grooves and steps, 30-282 nm deep. The contact of cells with the patterned surface activa...

journal_title：Experimental cell research

authors： Wójciak-Stothard B,Curtis A,Monaghan W,MacDonald K,Wilkinson C

更新日期：1996-03-15 00:00:00

abstract：:Genomic resources are indispensable for biological investigations in model organisms. In recent years, a number of genomic resources including a full genome assembly, extensive transcriptomic data, as well as genome editing has been developed for the axolotl, a classical model organism for developmental, neurobiologic...

journal_title：Experimental cell research

authors： Nowoshilow S,Tanaka EM

更新日期：2020-09-01 00:00:00

abstract：:We previously identified a novel centrosomal protein CPAP, which carries a 112-residue motif that is essential for microtubule destabilization. In this report, we define both the microtubule (MT) binding and destabilizing domains in human CPAP and analyze the mutations that affect its MT-destabilizing activity. Analys...

journal_title：Experimental cell research

authors： Hsu WB,Hung LY,Tang CJ,Su CL,Chang Y,Tang TK

更新日期：2008-08-15 00:00:00

abstract：:Approx. 35% of the DNA of cultured cells from the cactus mouse, Peromuscus eremicus, is contained in highly condensed constitutive heterochromatin which can be visualized in metaphase chromosomes stained by the C-band technique. Previous studies have shown this constitutive heterochromatin to contain a large proportio...

journal_title：Experimental cell research

authors： Halleck MS,Schlegel RA

更新日期：1983-09-01 00:00:00

abstract：:Breast tumor cells enter the bloodstream long before the development of clinically evident metastasis. However, the early presence of such bloodborne cells predicts poor patient outcome. Nearly 90% of human breast tumors arise as carcinomas from mammary epithelial cells, so it is important to study how these cells res...

journal_title：Experimental cell research

authors： Whipple RA,Cheung AM,Martin SS

更新日期：2007-04-15 00:00:00

abstract：:We found that a transient rise in cGMP levels, which was closely associated with the Ca2+ influx, occurred concomitant with the onset of myoblast fusion. The Ca2+ channel blocker D600 decreased both the cell fusion and the normal rise in cGMP levels. In contrast, the Ca2+ ionophore A23187 transiently increased cGMP le...

journal_title：Experimental cell research

authors： Choi SW,Baek MY,Kang MS

更新日期：1992-03-01 00:00:00

abstract：:We have studied the morphological alterations of the lysosomal compartment in rat hepatocytes following intraperitoneal administration of leupeptin, using electron microscopy and cytochemical techniques. At 30 min after the injection, autophagic vacuoles (autophagosomes and autolysosomes), containing cytoplasmic organ...

journal_title：Experimental cell research

authors： Ishikawa T,Furuno K,Kato K

更新日期：1983-03-01 00:00:00

abstract：:We previously demonstrated that the Tg737 gene plays a critical role in the carcinogenesis of hepatocellular carcinoma (HCC). However, few systematic investigations have focused on the biological function of Tg737 in the invasion and migration of liver cancer stem cells (LCSCs) and on its clinical significance. In thi...

journal_title：Experimental cell research

authors： You N,Tan Y,Zhou L,Huang X,Wang W,Wang L,Wu K,Mi N,Li J,Zheng L

更新日期：2017-09-15 00:00:00

abstract：:An attempt was made to determine the role of metabolic acidification of pericellular medium in regulating cell proliferation. A method of measuring the pH of pericellular medium at a distance exceeding a Debye radius of 5-10 A from the cell surface (pHp) was developed. The values of pHp and pH measured in the medium a...

journal_title：Experimental cell research

authors： Akatov VS,Lezhnev EI,Vexler AM,Kublik LN

更新日期：1985-10-01 00:00:00