当前位置： SCI文献检索 > EXPERIMENTAL CELL RESEARCH期刊下所有文献 > Pix1 and Pix2 are novel WD40 microtubule-associated proteins that colocalize with mitochondria in Xenopus germ plasm and centrosomes in human cells.

Pix1 and Pix2 are novel WD40 microtubule-associated proteins that colocalize with mitochondria in Xenopus germ plasm and centrosomes in human cells.

Abstract：

:In many animals, the germ line develops from a distinct mitochondria-rich region of embryonic cytoplasm called the germ plasm. However, the protein composition of germ plasm and its formation remain poorly understood, except in Drosophila. Here, we show that Xpat, a recently identified protein component of Xenopus germ plasm, interacts via its C-terminal domain with a novel protein, xPix1. Xpat and xPix1 are co-expressed in ovaries, eggs and early embryos and colocalize to the mitochondrial cloud and germ plasm in stage I and stage VI oocytes, respectively. Although Xpat appears unique to Xenopus, Pix proteins, which contain an N-terminal WD40 domain and C-terminal coiled-coil, are widely conserved. In humans, two proteins, Pix1 and Pix2, are expressed at varying levels in different cancer cell lines. Importantly, as well as localizing to mitochondria, human Pix proteins localize to centrosomes and associate with microtubules in vitro and in vivo. Although, Pix proteins are stably expressed through the cell cycle, Pix2 concentrates on microtubule structures in mitosis and microinjection of Pix antibodies interferes with cell division. Based on these data, we propose that Pix1 and Pix2 are microtubule-associated adaptor proteins that likely contribute to a range of developmental and cell division processes.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Hames RS,Hames R,Prosser SL,Euteneuer U,Lopes CA,Moore W,Woodland HR,Fry AM

doi

10.1016/j.yexcr.2007.10.019

subject

Has Abstract

pub_date

2008-02-01 00:00:00

pages

574-89

issue

3

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(07)00494-6

journal_volume

314

pub_type

杂志文章

相关文献

EXPERIMENTAL CELL RESEARCH文献大全
  • High glucose increases glomerular filtration barrier permeability by activating protein kinase G type Iα subunits in a Nox4-dependent manner.

    abstract：:Hyperglycemia is a primary factor that disturbs podocyte function in the glomerular filtration process; this disturbance leads to the development of diabetic nephropathy, and ultimately, renal failure. Podocyte function may also be altered by biological agents that modify protein kinase activity, including the cGMP-ac...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2013.09.005

    authors： Piwkowska A,Rogacka D,Audzeyenka I,Angielski S,Jankowski M

    更新日期：2014-01-01 00:00:00

  • Endothelial nitric oxide synthase is segregated from caveolin-1 and localizes to the leading edge of migrating cells.

    abstract：:The enzyme endothelial Nitric Oxide Synthase (eNOS) is involved in key physiological and pathological processes, including cell motility and apoptosis. It is widely believed that at the cell surface eNOS is localized in caveolae, where caveolin-1 negatively regulates its activity, however, there are still uncertaintie...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2005.12.014

    authors： Bulotta S,Cerullo A,Barsacchi R,De Palma C,Rotiroti D,Clementi E,Borgese N

    更新日期：2006-04-01 00:00:00

  • Berberine regulates neurite outgrowth through AMPK-dependent pathways by lowering energy status.

    abstract：:As a widely used anti-bacterial agent and a metabolic inhibitor as well as AMP-activated protein kinase (AMPK) activator, berberine (BBR) has been shown to cross the blood-brain barrier. Its efficacy has been investigated in various disease models of the central nervous system. Neurite outgrowth is critical for nervou...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2015.04.006

    authors： Lu J,Cao Y,Cheng K,Xu B,Wang T,Yang Q,Yang Q,Feng X,Xia Q

    更新日期：2015-06-10 00:00:00

  • Fiber DNA studies of premeiotic mouse spermatogenesis.

    abstract：:The technique of fiber DNA measurement was used to study the possibility that the lengthening of the DNA "S" phase previously reported for mouse premeiotic spermatogonia was due to a reduced number of initiation sites. The mean replicon size of neonatal mouse preleptotene cells was similar to sizes reported for adult ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(83)90130-1

    authors： Jagiello G,Sung WK,Van't Hof J

    更新日期：1983-07-01 00:00:00

  • Structure in the amphibian germinal vesicle.

    abstract：:The germinal vesicle (GV) of Xenopus laevis is an enormous nucleus that contains 18 giant lampbrush chromosomes and thousands of inclusions. The inclusions are primarily of three types: approximately 1500 extrachromosomal nucleoli, 50-100 Cajal bodies, and several thousand B-snurposomes, which correspond to speckles o...

    journal_title：Experimental cell research

    pub_type： 杂志文章,评审

    doi：10.1016/j.yexcr.2004.03.017

    authors： Gall JG,Wu Z,Murphy C,Gao H

    更新日期：2004-05-15 00:00:00

  • Exchange of chemical signals between cardiac cells. Fundamental role on cell communication and metabolic cooperation.

    abstract：:The exchange of chemical signals between cardiac cells and its relevance for cell communication and metabolic cooperation was reviewed. The role of gap junctions on the transfer of chemical information was discussed as well as the different factors involved in its regulation including changes in cell volume, high gluc...

    journal_title：Experimental cell research

    pub_type： 杂志文章,评审

    doi：10.1016/j.yexcr.2016.05.009

    authors： De Mello WC

    更新日期：2016-08-01 00:00:00

  • The impact of maternal serum on development of enolase activity in fetal rat brain cell culture.

    abstract：:The effect of gestational age on serum-mediated changes in enolase activity was tested in a fetal rat brain cell culture. After 6 days exposure to graded concentrations (10 and 20%) of nonpregnant female rat sera, enolase activity in brain cell cultures increased from 2.83 +/- 0.03 to 3.74 +/- 0.19 mumol/min/mg protei...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(90)90298-o

    authors： Shambaugh GE 3rd,Clough D,Munari L,Koehler RR,Radosevich JA

    更新日期：1990-02-01 00:00:00

  • Resveratrol decreases TNFα-induced ICAM-1 expression and release by Sirt-1-independent mechanism in intestinal myofibroblasts.

    abstract：:Up-regulation of intercellular adhesion molecule-1 (ICAM-1) and its soluble form are involved in the chronic inflammation. For the first time, we demonstrated that resveratrol (RE), a natural polyphenol with antioxidant and anti-inflammatory properties, reduces the increase of expression and release of ICAM-1, due to ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2019.06.024

    authors： Domazetovic V,Bonanomi AG,Stio M,Vincenzini MT,Iantomasi T

    更新日期：2019-09-15 00:00:00

  • Changes in elastin-binding proteins during the phenotypic transition of rabbit arterial smooth muscle cells in primary culture.

    abstract：:Elastin, a major constituent of the media, may play an important role in the attachment, migration, and phenotypic properties of vascular smooth muscle cells (SMC). We examined binding proteins and binding sites on elastin in rabbit arterial SMC in primary culture. Freshly isolated cells on alpha-elastin underwent phe...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1995.1164

    authors： Yamamoto K,Aoyagi M,Yamamoto M

    更新日期：1995-05-01 00:00:00

  • Cellular contraction of collagen lattices is inhibited by nonenzymatic glycation.

    abstract：:High glucose concentrations associated with diabetes have been shown to cause the nonenzymatic modification of proteins. Reducing sugars covalently bind to free amine groups, undergo Amadori rearrangements, and crosslink with other glucose-modified proteins. Crosslinking of type I collagen by incubation with different...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1996.0308

    authors： Howard EW,Benton R,Ahern-Moore J,Tomasek JJ

    更新日期：1996-10-10 00:00:00

  • Detection in BHK cells of a precursor form for lamin A.

    abstract：:Lamins are structural proteins found in the fibrous lamina underlining the nuclear envelope. In vitro translation of polyadenylated RNA or polysomes followed by immunoprecipitation with a serum raised against BHK nuclear matrix proteins showed that lamin A (72 kD) is synthesized as a high molecular weight precursor (7...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(85)90084-9

    authors： Dagenais A,Bibor-Hardy V,Laliberté JF,Royal A,Simard R

    更新日期：1985-12-01 00:00:00

  • Vascular smooth muscle cell detachment from elastin and migration through elastic laminae is promoted by chondroitin sulfate-induced "shedding" of the 67-kDa cell surface elastin binding protein.

    abstract：:Impaired elastin fiber assembly is observed in the fetal ductus arteriosus (DA), associated with a reduced concentration of elastin binding protein (EBP), a 67-kDa galactolectin. It is also seen in cultured aortic (Ao) smooth muscle cells (SMC) following the release of the EBP by glycosaminoglycans rich in N-acetylgal...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(92)90008-v

    authors： Hinek A,Boyle J,Rabinovitch M

    更新日期：1992-12-01 00:00:00

  • Human Kupffer cell recognition and phagocytosis of apoptotic peripheral blood lymphocytes.

    abstract：:Cells undergoing apoptosis are recognized and rapidly phagocytosed by macrophages before their degradation, thus preventing the inflammatory reaction and protecting tissues from the damaging effects of released potentially harmful intracellular contents. In spite of growing interest in the mechanisms leading to the en...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1996.0123

    authors： Falasca L,Bergamini A,Serafino A,Balabaud C,Dini L

    更新日期：1996-04-10 00:00:00

  • Characteristics of intracellular transferrin produced by human fibroblasts: its posttranscriptional regulation and association with tubulin.

    abstract：:Transferrin (Tf), an iron-binding protein, was investigated in the cultured human fibroblast which is a major cell type in connective tissues. Tf is a major iron-transporting protein and has an important role in iron metabolism. Using two-dimensional gel electrophoresis, we demonstrated the eight subtypes of Tf produc...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1998.4080

    authors： Kondo T,Sakaguchi M,Namba M

    更新日期：1998-07-10 00:00:00

  • Polymorphonuclear leukocyte-myocyte interaction: an early event in collar-induced rabbit carotid intimal thickening.

    abstract：:The importance of mononuclear leukocyte (MO) adhesion to dysfunctional endothelium and migration to the subendothelial space in the early phases of atherogenesis is well established. Few studies have addressed the relevance of polymorphonuclear leukocytes (PMNs) in the context of evolving lesions, and nothing is known...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2001.5459

    authors： Donetti E,Baetta R,Comparato C,Altana C,Sartore S,Paoletti R,Castano P,Gabbiani G,Corsini A

    更新日期：2002-04-01 00:00:00

  • MPF amplification in Xenopus oocyte extracts depends on a two-step activation of cdc25 phosphatase.

    abstract：:The activation of Cdc2 kinase induces the entry into M-phase of all eukaryotic cells. We have developed a cell-free system prepared from prophase-arrested Xenopus oocytes to analyze the mechanism initiating the all-or-none activation of Cdc2 kinase. Inhibition of phosphatase 2A, the major okadaic acid-sensitive Ser/Th...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1998.4220

    authors： Karaïskou A,Cayla X,Haccard O,Jessus C,Ozon R

    更新日期：1998-11-01 00:00:00

  • Expression of lamin A mutated in the carboxyl-terminal tail generates an aberrant nuclear phenotype similar to that observed in cells from patients with Dunnigan-type partial lipodystrophy and Emery-Dreifuss muscular dystrophy.

    abstract：:Autosomal dominantly inherited missense mutations in lamins A and C cause familial partial lipodystrophy of the Dunnigan-type (FPLD), and myopathies including Emery-Dreifuss muscular dystrophy (EDMD). While mutations responsible for FPLD are restricted to the carboxyl-terminal tails, those responsible for EDMD are spr...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2002.5669

    authors： Favreau C,Dubosclard E,Ostlund C,Vigouroux C,Capeau J,Wehnert M,Higuet D,Worman HJ,Courvalin JC,Buendia B

    更新日期：2003-01-01 00:00:00

  • Role of YAP/TAZ in cell-matrix adhesion-mediated signalling and mechanotransduction.

    abstract：:Signalling from the extracellular matrix (ECM) is a fundamental cellular input that sustains proliferation, opposes cell death and regulates differentiation. Through integrins, cells perceive both the chemical composition and physical properties of the ECM. In particular, cell behaviour is profoundly influenced by the...

    journal_title：Experimental cell research

    pub_type： 杂志文章,评审

    doi：10.1016/j.yexcr.2015.10.034

    authors： Dupont S

    更新日期：2016-04-10 00:00:00

  • Correlation between transglutaminase activity and polyamine levels in human neuroblastoma cells. Effect of retinoic acid and alpha-difluoromethylornithine.

    abstract：:The human neuroblastoma cell line SK-N-BE can be induced to differentiate by retinoic acid (RA) or by alpha-difluoromethylornithine (DFMO). The former inducer produces neurite outgrowth, 60% reduction of growth rate, overexpression of neural antigens, and enhanced gamma-aminobutyric acid (GABA) and acetylcholinesteras...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(88)90281-9

    authors： Melino G,Farrace MG,Ceru' MP,Piacentini M

    更新日期：1988-12-01 00:00:00

  • Eps8 is recruited to lysosomes and subjected to chaperone-mediated autophagy in cancer cells.

    abstract：:Eps8 controls actin dynamics directly through its barbed end capping and actin-bundling activity, and indirectly by regulating Rac-activation when engaged into a trimeric complex with Eps8-Abi1-Sos1. Recently, Eps8 has been associated with promotion of various solid malignancies, but neither its mechanisms of action n...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2010.02.020

    authors： Welsch T,Younsi A,Disanza A,Rodriguez JA,Cuervo AM,Scita G,Schmidt J

    更新日期：2010-07-15 00:00:00

  • The role of phosphorylation in modulating beta 1 integrin localization.

    abstract：:The beta 1-integrin subunit localizes to focal contacts during F9 teratocarcinoma stem cell differentiation to parietal endoderm. Concomitantly, this integrin subunit becomes dephosphorylated at serine 790, the only serine in the well-conserved cytoplasmic domain of beta 1. We hypothesized that this dephosphorylation ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1996.0002

    authors： Barreuther MF,Grabel LB

    更新日期：1996-01-10 00:00:00

  • In vitro replication and differentiation of normal human oral keratinocytes.

    abstract：:The replication kinetics and cytological changes of normal human oral keratinocytes (NHOK) isolated from the basal surface of oral epithelial sheet and cultured as dispersed cells in low (0.15 mM) Ca(2+) medium without serum were analyzed. Replicating NHOK were quantitated by cell count and identified by [(3)H]thymidi...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2000.4943

    authors： Kang MK,Bibb C,Baluda MA,Rey O,Park NH

    更新日期：2000-08-01 00:00:00

  • The MAPK ERK5, but not ERK1/2, inhibits the progression of monocytic phenotype to the functioning macrophage.

    abstract：:Intracellular signaling pathways present targets for pharmacological agents with potential for treatment of neoplastic diseases, with some disease remissions already recorded. However, cellular compensatory mechanisms usually negate the initial success. For instance, attempts to interrupt aberrant signaling downstream...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2014.10.003

    authors： Wang X,Pesakhov S,Harrison JS,Kafka M,Danilenko M,Studzinski GP

    更新日期：2015-01-01 00:00:00

  • p75NTR enhances PC12 cell tumor growth by a non-receptor mechanism involving downregulation of cyclin D2.

    abstract：:p75NTR is a member of the tumor necrosis superfamily of proteins which is variably associated with induction of apoptosis and proliferation. Cyclin D2 is one of the mediators of cellular progression through G1 phase of the cell cycle. The present study demonstrates the inverse relationship between expression of cyclin...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2006.06.029

    authors： Fritz MD,Mirnics ZK,Nylander KD,Schor NF

    更新日期：2006-10-15 00:00:00

  • GADD34 mediates cytoprotective autophagy in mutant huntingtin expressing cells via the mTOR pathway.

    abstract：:Increased protein aggregation and altered cell signaling accompany many neurodegenerative diseases including Huntington's disease (HD). Cell stress is counterbalanced by signals mediating cell repair but the identity of these are not fully understood. We show here that the mammalian target of rapamycin (mTOR) pathway ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2011.08.020

    authors： Hyrskyluoto A,Reijonen S,Kivinen J,Lindholm D,Korhonen L

    更新日期：2012-01-01 00:00:00

  • Cellular responses to disruption of the permeability barrier in a three-dimensional organotypic epidermal model.

    abstract：:Repeated injury to the stratum corneum of mammalian skin (caused by friction, soaps, or organic solvents) elicits hyperkeratosis and epidermal thickening. Functionally, these changes serve to restore the cutaneous barrier and protect the organism. To better understand the molecular and cellular basis of this response,...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2007.04.021

    authors： Ajani G,Sato N,Mack JA,Maytin EV

    更新日期：2007-08-15 00:00:00

  • Characterization of peroxisomes in Chinese hamster ovary cells in culture.

    abstract：:In order to explore the potential value of Chinese hamster ovary (CHO) cells for the isolation of peroxisomal mutants defective in the peroxisomal fatty acid oxidation system, some characteristics of their peroxisomes were studied. Catalase was detected biochemically and histochemically in peroxisome-like particles in...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(85)90503-8

    authors： Santos MJ,Garrido J,Oliver C,Robbins AR,Leighton F

    更新日期：1985-11-01 00:00:00

  • Protein kinase C regulates the phosphorylation and oligomerization of ERM binding phosphoprotein 50.

    abstract：:Ezrin-Radixin-Moesin (ERM) binding phosphoprotein 50 (EBP50, a.k.a. NHERF-1) is a scaffold protein essential for the localization and coordinated activity of apical transporters, enzymes and receptors in epithelial cells. EBP50 acts via multiple protein binding interactions, including oligomerization through interacti...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2005.02.011

    authors： Fouassier L,Nichols MT,Gidey E,McWilliams RR,Robin H,Finnigan C,Howell KE,Housset C,Doctor RB

    更新日期：2005-05-15 00:00:00

  • Identification of a brain-specific 27/26-kDa extracellular protein with the monoclonal antibody to differentiated PC12h pheochromocytoma cells.

    abstract：:We have searched for brain-specific extracellular molecules using a library of monoclonal antibodies against surface antigens of differentiated PC12h cells. One of the monoclonal antibodies, PCH42-14, recognized a 27/26-kDa protein of 10-week-old rat brain on immunoblotting. PCH42-14 antigen was detected only in brain...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(91)90549-a

    authors： Ishikawa M,Kuroda Y,Kobayashi K,Sawada H,Hayashi M

    更新日期：1991-03-01 00:00:00

  • Effects of polyamines on the first division cycle of Xenopus laevis eggs.

    abstract：:The involvement of polyamines in cytokinesis has been examined in eggs of the amphibian X. laevis. Microinjection of spermine or spermidine into unfertilized eggs induced a shortening of the first division cycle and early formation of cleavage-like constrictions. Eggs were activated by injection and developed furrows ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(84)90593-7

    authors： Grant NJ,Aimar C,Oriol-Audit C

    更新日期：1984-02-01 00:00:00