Fiber DNA studies of premeiotic mouse spermatogenesis.

abstract：:Two SV40-transformed human cell lines, GM637, derived from a normal human subject, and GM5849, derived from a patient with ataxia-telangiectasia (A-T), were grown in increasing concentrations of the cytotoxic agent methotrexate (MTX). The GM637 line was naturally more resistant to methotrexate than was GM5849 and, ove...

journal_title：Experimental cell research

authors： Hahn P,Kapp LN,Painter RB

更新日期：1987-01-01 00:00:00

abstract：:Glycosaminoglycans (heparins, dermatan sulfate, chondroitin sulfate) with different structures and physicochemical properties were evaluated for their capacity to influence proliferation and differentiation of U-937 cell line. The contrasting and specific effects of glycosaminoglycans (depending on their structures an...

journal_title：Experimental cell research

authors： Volpi N,Petrini M,Conte A,Valentini P,Venturelli T,Bolognani L,Ronca G

更新日期：1994-11-01 00:00:00

abstract：:hnRNP I, also referred to as polypyrimidine tract binding protein, is one of the proteins associated with nascent pre-mRNA in the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes. As for all karyophilic proteins, the nuclear import of hnRNP proteins requires specific sequence determinants that in many instanc...

journal_title：Experimental cell research

authors： Romanelli MG,Weighardt F,Biamonti G,Riva S,Morandi C

更新日期：1997-08-25 00:00:00

abstract：:Autophagy is a cellular pathway involved in protein and organelle degradation. It is relevant to many types of cellular homeostasis and human diseases. High level of glucose is known to inflict podocyte injury, but little is reported about the relationship between high concentrations of glucose and autophagy in these ...

journal_title：Experimental cell research

authors： Ma T,Zhu J,Chen X,Zha D,Singhal PC,Ding G

更新日期：2013-04-01 00:00:00

abstract：:Liver membrane glycoproteins with affinity for immobilized collagen type I were subjected to preparative sodium dodecyl sulfate-polyacrylamide gel electrophoresis (SDS-PAGE) followed by electroelution of the separated proteins. Electroeluted glycoproteins with ability to neutralize the inhibitory effect of anti-CollCA...

journal_title：Experimental cell research

authors： Gullberg D,Terracio L,Rubin K

更新日期：1988-04-01 00:00:00

abstract：:Human endothelial cells, when suspended within three-dimensional collagen matrices, develop intracellular vacuoles that coalesce to form capillary lumens and tubes. Vacuole and lumen formation are completely dependent on the collagen-binding integrin alpha 2 beta 1, while other endothelial cell integrins had no appare...

journal_title：Experimental cell research

authors： Davis GE,Camarillo CW

更新日期：1996-04-10 00:00:00

abstract：:We have established, in continuous cell culture, a spontaneously immortalized chicken embryo fibroblast (CEF) cell line (DF-1) as well as several other immortal CEF cell lines. The immortal DF-1 cells divided more rapidly than primary and other immortal CEF cells. To identify the genes involved in rapidly dividing DF-...

journal_title：Experimental cell research

authors： Kim H,You S,Kim IJ,Farris J,Foster LK,Foster DN

更新日期：2001-05-01 00:00:00

abstract：:Sensitivity of tumor cells to treatment with anticancer drugs depends on expression and function of antiapoptotic and antioxidant proteins. The goal of our study was to determine the functional role of the novel antioxidant protein Peroxiredoxin V (PrxV), in protection of human lung carcinoma cell lines against apopto...

journal_title：Experimental cell research

authors： Kropotov A,Gogvadze V,Shupliakov O,Tomilin N,Serikov VB,Tomilin NV,Zhivotovsky B

更新日期：2006-09-10 00:00:00

abstract：:Treatment with ionic detergents of nuclei isolated from various continuously growing cell lines generally yields chromatin samples of high viscosity. Extensive treatment with nuclease-free proteinase K or pronase solubilized the viscous lysates with > 90% of the DNA migrating at approximately 50 kb. Freshly prepared h...

journal_title：Experimental cell research

authors： Szabó G Jr

更新日期：1995-12-01 00:00:00

abstract：:A respiratory epithelial cell line M3E3/C3, derived from fetal Syrian hamster lung, grew very slowly in RPMI 1640 medium with serum concentrations as low as 1.5%. Under these conditions the simultaneous action of epidermal growth factor (EGF) and vitamin A (retinol) was apparently a combination of growth enhancement a...

journal_title：Experimental cell research

authors： Böning W,Kakunaga T,Emura M,Mohr U

更新日期：1986-03-01 00:00:00

abstract：:The abundant heterogeneous nuclear ribonucleoprotein M (hnRNP M) is able to associate with early spliceosomes and to influence splicing patterns of specific pre-mRNAs. Here, by a combination of immunoprecipitation and pull-down assays, we have identified PSF (polypyrimidine tract-binding protein-associated splicing fa...

journal_title：Experimental cell research

authors： Marko M,Leichter M,Patrinou-Georgoula M,Guialis A

更新日期：2010-02-01 00:00:00

abstract：:Co-cultures of lutropin receptor (LHR) positive and negative Leydig cells were used to test the hypothesis that the LHR provokes phosphorylation of the extracellular regulated kinases (ERK1/2) using intracellular and intercellular pathways. Addition of hCG to MA-10 cells (LHR positive) stimulates phosphorylation of th...

journal_title：Experimental cell research

authors： Shiraishi K,Ascoli M

更新日期：2008-01-01 00:00:00

abstract：:We have compared the in vitro replicative life span and characteristics of immortalization of skin fibroblast cultures derived from ad libitum-fed and caloric-restricted Fischer 344 rats of 6, 24, and 29 months of age. Cells from all 6-, 24-, and 29-month-old animals showed a gradual decline in proliferative potential...

journal_title：Experimental cell research

authors： Pignolo RJ,Masoro EJ,Nichols WW,Bradt CI,Cristofalo VJ

更新日期：1992-07-01 00:00:00

abstract：:In the acute promyelocytic leukemia cell line NB4, Bcl-2 downregulation occurred as a late event of retinoid-induced differentiation. In the maturation-resistant NB4-R1 subclone, retinoids failed to downregulate Bcl-2 even in the situation of apoptosis massively induced by pan-agonists and RXR-selective agonists. We o...

journal_title：Experimental cell research

authors： Bruel A,Karsenty E,Schmid M,McDonnell TJ,Lanotte M

更新日期：1997-06-15 00:00:00

abstract：:Alpha-Smooth muscle actin is one of the molecular markers for a phenotype of vascular smooth muscle cells, because the actin is a major isoform expressed in vascular smooth muscle cells and its expression is upregulated during differentiation. Here, we first demonstrate that the phenotype-dependent expression of this ...

journal_title：Experimental cell research

authors： Saga H,Kimura K,Hayashi K,Gotow T,Uchiyama Y,Momiyama T,Tadokoro S,Kawashima N,Jimbou A,Sobue K

更新日期：1999-02-25 00:00:00

abstract：:The abilities of eight extracellular matrix proteins, fibronectin, vitronectin, laminin, and collagen types I, II, III, IV, and V to bind insulin were examined by binding studies with insulin conjugated with peroxidase. At a physiological pH and ionic strength, type V collagen bound to insulin most strongly. The other...

journal_title：Experimental cell research

authors： Yaoi Y,Hashimoto K,Takahara K,Kato I

更新日期：1991-06-01 00:00:00

abstract：:Endothelial cell-selective adhesion molecule (ESAM) is an immunoglobulin-like transmembrane protein associated with endothelial tight junctions (TJ). Based on a yeast two-hybrid screen, we have identified the membrane-associated guanylate kinase protein MAGI-1 as an intracellular binding partner of ESAM. MAGI-1 is a m...

journal_title：Experimental cell research

authors： Wegmann F,Ebnet K,Du Pasquier L,Vestweber D,Butz S

更新日期：2004-10-15 00:00:00

abstract：:The role of p53 in transcriptional activation of genes involved in cell cycle progression is well established. However, the wide range of functions attributed to this gene suggests that some of them might be unrelated to transcription. Here we investigated p53 localization and recruitment to chromatin during Xenopus e...

journal_title：Experimental cell research

authors： Tchang F,Méchali M

更新日期：1999-08-25 00:00:00

abstract：:Repeated injury to the stratum corneum of mammalian skin (caused by friction, soaps, or organic solvents) elicits hyperkeratosis and epidermal thickening. Functionally, these changes serve to restore the cutaneous barrier and protect the organism. To better understand the molecular and cellular basis of this response,...

journal_title：Experimental cell research

authors： Ajani G,Sato N,Mack JA,Maytin EV

更新日期：2007-08-15 00:00:00

abstract：:The post-traumatic hypertrophic scar (HS) is a fibrotic disease with excessive extracellular matrix (ECM) production by fibroblasts in response to tissue injury. Although dysregulation of miRNAs is known to be involved in a variety of pathophysiologic processes, the role of miRNA in hypertrophic scar formation is uncl...

journal_title：Experimental cell research

authors： Zhou R,Zhang Q,Zhang Y,Fu S,Wang C

更新日期：2015-12-10 00:00:00

abstract：:Cellular senescence is a state of irreversible cell cycle arrest in which normal cells at the end of their lifespan fail to enter into DNA synthesis upon serum or growth factor stimulation. We examined whether proteins required for G1/S cell cycle progression were irreversibly down-regulated in senescent human fibrobl...

journal_title：Experimental cell research

authors： Afshari CA,Vojta PJ,Annab LA,Futreal PA,Willard TB,Barrett JC

更新日期：1993-12-01 00:00:00

abstract：:The remodeling of extracellular matrix is a crucial mechanism in tendon development and the proliferation of fibroblasts is a key factor in this process. The purpose of this study was to further elucidate the role of TIEG1 in mediating important tenocyte properties throughout the aging process. Wildtype and TIEG1 knoc...

journal_title：Experimental cell research

authors： Haddad O,Gumez L,Hawse JR,Subramaniam M,Spelsberg TC,Bensamoun SF

更新日期：2011-07-15 00:00:00

abstract：:Breast cancer is a common malignancy and a major cause of death in women worldwide. The immunomodulatory role of B cells is being increasingly recognized in autoimmune diseases and cancers. In recent years, immunotherapeutic strategies that upregulate the patient's own antitumor T cell responses have shown promise in ...

journal_title：Experimental cell research

authors： Zhu S,Wang X,Wang J,Lin J,Cong Y,Qiao G

更新日期：2017-12-01 00:00:00

abstract：:High-grade gliomas (HGG), are the most common aggressive brain tumours in adults. Inhibitors targeting growth factor signalling pathways in glioma have shown a low clinical response rate. To accurately evaluate response to targeted therapies further in vitro studies are necessary. Growth factor pathway expression usin...

journal_title：Experimental cell research

authors： Kinsella P,Howley R,Doolan P,Clarke C,Madden SF,Clynes M,Farrell M,Amberger-Murphy V

更新日期：2012-03-10 00:00:00

abstract：:Xenopus early embryos are transcriptionally quiescent until the midblastula transition (MBT). We have examined the question of whether the absence of rRNA synthesis is related to a deficiency in the RNA polymerase I (pol I) transcription machinery. Previously we have demonstrated that the maternally provided pol I tra...

journal_title：Experimental cell research

authors： Bell P,Scheer U

更新日期：1999-04-10 00:00:00

abstract：:Maturation of MC3T3-E1 osteoblast cells in vitro can be divided into three major stages, namely, proliferation, differentiation, and mineralization. Our microarray analysis identified genes differentially expressed between proliferating and differentiated MC3T3-E1 osteoblastic cells. Immunohistochemical analyses of RN...

journal_title：Experimental cell research

authors： Gao Y,Ganss BW,Wang H,Kitching RE,Seth A

更新日期：2005-03-10 00:00:00

abstract：:A highly sensitive, kinetically unambiguous assay for alpha-factor-induced delay of cell passage through the "start" step of cell division in yeast is presented. The assay employs perfusion with periodic microscopy to monitor the bud emergence kinetics on the 20% of cells within an exponentially growing population whi...

journal_title：Experimental cell research

authors： Moore SA

更新日期：1987-08-01 00:00:00

abstract：:The retinoblastoma tumor suppressor protein (RB) is activated/dephosphorylated to mediate cell cycle inhibition in response to antimitogenic signals. To elucidate the mode of RB action at this critical transition, we utilized cell lines that can be induced to express a constitutively active allele of RB (PSM-RB). As e...

journal_title：Experimental cell research

authors： Angus SP,Fribourg AF,Markey MP,Williams SL,Horn HF,DeGregori J,Kowalik TF,Fukasawa K,Knudsen ES

更新日期：2002-06-10 00:00:00

abstract：:The small GTPases Rho, Rac, and Cdc42 regulate the actin cytoskeleton in all eukaryotic cells. In this study we have evaluated the effect of cholesterol oxides (7-ketocholesterol and 25-hydroxycholesterol) on cell migration, cell adhesion, and cytoskeletal organisation of lens epithelial cells (LEC). Effects of choles...

journal_title：Experimental cell research

authors： Girão H,Pereira P,Ramalho J,Quinlan R,Prescott A

更新日期：2003-12-10 00:00:00

abstract：:The radula of the limpet, Cellana toreuma, consists of a continuous series of teeth in various stages of iron biomineralization. The major iron-binding protein of the limpet's iron-containing granule (siderosome) has been purified and identified as ferritin. Limpet ferritin has a M(r) of 575 kDa and can be resolved in...

journal_title：Experimental cell research

authors： Lu HK,Huang CM,Li CW

更新日期：1995-07-01 00:00:00