Abstract：
:Alpha-Smooth muscle actin is one of the molecular markers for a phenotype of vascular smooth muscle cells, because the actin is a major isoform expressed in vascular smooth muscle cells and its expression is upregulated during differentiation. Here, we first demonstrate that the phenotype-dependent expression of this actin in visceral smooth muscles is quite opposite to that in vascular smooth muscles. This actin isoform is not expressed in adult chicken visceral smooth muscles including gizzard, trachea, and intestine except for the inner layer of intestinal muscle layers, whereas its expression is clearly detected in these visceral smooth muscles at early stages of the embryo (10-day-old embryo) and is developmentally downregulated. In cultured gizzard smooth muscle cells maintaining a differentiated phenotype, alpha-smooth muscle actin is not detected while its expression dramatically increases during serum-induced dedifferentiation. Promoter analysis reveals that a sequence (-238 to -219) in the promoter region of this actin gene acts as a novel negative cis-element. In conclusion, the phenotype-dependent expression of alpha-smooth muscle actin would be regulated by the sum of the cooperative contributions of the negative element and well-characterized positive elements, purine-rich motif, and CArG boxes and their respective transacting factors.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Saga H,Kimura K,Hayashi K,Gotow T,Uchiyama Y,Momiyama T,Tadokoro S,Kawashima N,Jimbou A,Sobue Kdoi
10.1006/excr.1998.4339keywords:
subject
Has Abstractpub_date
1999-02-25 00:00:00pages
279-92issue
1eissn
0014-4827issn
1090-2422pii
S0014-4827(98)94339-7journal_volume
247pub_type
杂志文章abstract：:In preliminary experiments it was established that the hypertrophic and hyperplastic responses of neonatal cardiac myocytes in culture were associated with enhanced expression of IGF-1 and IGF-1 receptors in these cells. Therefore, to determine the role of IGF-1 receptors on myocyte growth, cells were exposed to antis...
journal_title：Experimental cell research
pub_type： 杂志文章
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pub_type： 杂志文章,评审
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journal_title：Experimental cell research
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doi：10.1006/excr.1996.0213
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pub_type： 杂志文章
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journal_title：Experimental cell research
pub_type： 杂志文章
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pub_type： 杂志文章
doi：10.1016/0014-4827(91)90554-8
更新日期：1991-03-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0014-4827(85)90230-7
更新日期：1985-09-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1996.0071
更新日期：1996-02-25 00:00:00
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journal_title：Experimental cell research
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更新日期：1996-10-10 00:00:00
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