当前位置： SCI文献检索 > EXPERIMENTAL CELL RESEARCH期刊下所有文献 > Antibodies against Tetrahymena 14-nm filament-forming protein recognize the replication band in Euplotes.

Antibodies against Tetrahymena 14-nm filament-forming protein recognize the replication band in Euplotes.

Abstract：

:Tetrahymena 14-nm filament-forming protein (49K protein) is a structural protein which is involved in activity of the pronuclei during conjugation (O. Numata, T. Sugai, and Y. Watanabe (1985) Nature (London) 314, 192-194). Using monoclonal and polyclonal antibodies, we here demonstrate the presence of a cross-reactive protein (CRP-49) within the macronuclear replication bands of Euplotes harpa and E. eurystomus which is recognized by anti-49K protein antibodies. Immunoblotting reveals that both monoclonal and polyclonal antibodies cross-react to a protein with an apparent molecular mass of 50 kDa in an E. harpa cell extract and to a protein of 49 kDa in a macronuclear extract of E. eurystomus. The antibodies used in this study have no effect upon in vitro DNA synthesis in the replication band of E. eurystomus.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Numata O,Tomiyoshi T,Kurasawa Y,Fu ZX,Takahashi M,Kosaka T,Chiba J,Watanabe Y

doi

10.1016/0014-4827(91)90554-8

subject

Has Abstract

pub_date

1991-03-01 00:00:00

pages

183-9

issue

1

eissn

0014-4827

issn

1090-2422

journal_volume

193

pub_type

杂志文章

相关文献

EXPERIMENTAL CELL RESEARCH文献大全
  • Changes in the DNA polymerase beta gene expression during development of lung, brain, and testis suggest an involvement of the enzyme in DNA recombination.

    abstract：:Changes in the expression pattern of DNA polymerase beta gene during rat lung, brain, and testis development have been investigated. A decrease in the level of beta-pol mRNA was observed during postnatal development of lung and brain. By contrast, an almost 20-fold increase in the level of beta-pol mRNA was observed d...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(90)90034-8

    authors： Nowak R,Woszczynski M,Siedlecki JA

    更新日期：1990-11-01 00:00:00

  • Identification and localization of a novel nucleolar protein of high molecular weight by a monoclonal antibody.

    abstract：:A monoclonal murine antibody (No-114) is described which reacts specifically with a polypeptide of molecular weight (Mr) 180 000 present in low-speed nuclear pellets from oocytes and somatic cells of Xenopus laevis and X. borealis and in isolated amplified nucleoli. Two-dimensional gel electrophoresis has revealed the...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(84)90604-9

    authors： Schmidt-Zachmann MS,Hügle B,Scheer U,Franke WW

    更新日期：1984-08-01 00:00:00

  • EAT/mcl-1 expression in the human embryonal carcinoma cells undergoing differentiation or apoptosis.

    abstract：:Differentiation and apoptosis are precisely regulated events in early embryogenesis. Retinoic acid-induced differentiation in the embryonal carcinoma (EC) cell line NCR-G3 triggers concurrent induction of apoptosis. Using this system, which serves as a model of early embryogenesis, the expression of various bcl-2-rela...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2001.5203

    authors： Sano M,Umezawa A,Abe H,Akatsuka A,Nonaka S,Shimizu H,Fukuma M,Hata J

    更新日期：2001-05-15 00:00:00

  • Genomic response to Wnt signalling is highly context-dependent--evidence from DNA microarray and chromatin immunoprecipitation screens of Wnt/TCF targets.

    abstract：:Wnt proteins are important regulators of embryonic development, and dysregulated Wnt signalling is involved in the oncogenesis of several human cancers. Our knowledge of the downstream target genes is limited, however. We used a chromatin immunoprecipitation-based assay to isolate and characterize the actual gene segm...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2009.06.021

    authors： Railo A,Pajunen A,Itäranta P,Naillat F,Vuoristo J,Kilpeläinen P,Vainio S

    更新日期：2009-10-01 00:00:00

  • CXCR4 and CXCL12 are inversely expressed in colorectal cancer cells and modulate cancer cell migration, invasion and MMP-9 activation.

    abstract：:Colorectal cancer (CRC) is characterized by a distinct metastatic pattern resembling chemokine-induced leukocyte trafficking. This prompted us to investigate expression, signal transduction and specific functions of the chemokine receptor CXCR4 in CRC cells and metastases. Using RT-PCR analysis and Western blotting, w...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2005.07.006

    authors： Brand S,Dambacher J,Beigel F,Olszak T,Diebold J,Otte JM,Göke B,Eichhorst ST

    更新日期：2005-10-15 00:00:00

  • p66Shc promotes HCC progression in the tumor microenvironment via STAT3 signaling.

    abstract：:The development of hepatocellular carcinoma (HCC) is strongly associated with chronic inflammation. p66Shc is an oxidase previously shown to promote androgen-independent cell growth through generation of reactive oxygen species. However, the importance and biologic functions of p66Shc in HCC are unclear. The clinical ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2019.111550

    authors： Huang P,Feng X,Zhao Z,Yang B,Fang T,Guo M,Xia J

    更新日期：2019-10-15 00:00:00

  • Differential expression of Raf-1 protooncogene in resting and activated human leukocyte populations.

    abstract：:In this study we examined by Northern blot analysis the expression of Raf-1 protooncogene in normal human peripheral blood leukocytes. Unlike thymocytes, circulating lymphocytes did not express appreciable levels of Raf-1 mRNA. In contrast, polymorphonuclear cells (PMN) had high levels of Raf-1 transcripts. Also densi...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(91)90366-3

    authors： Colotta F,Polentarutti N,Mantovani A

    更新日期：1991-06-01 00:00:00

  • Mammalian ChlR1 has a role in heterochromatin organization.

    abstract：:The ChlR1 DNA helicase, encoded by DDX11 gene, which is responsible for Warsaw breakage syndrome (WABS), has a role in sister-chromatid cohesion. In this study, we show that human ChlR1 deficient cells exhibit abnormal heterochromatin organization. While constitutive heterochromatin is discretely localized at perinucl...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2011.08.006

    authors： Inoue A,Hyle J,Lechner MS,Lahti JM

    更新日期：2011-10-15 00:00:00

  • Organization of early and late replicating DNA in human chromosome territories.

    abstract：:It has been suggested that DNA organized into replication foci during S-phase remains stably aggregated in non-S-phase cells and that these stable aggregates provide fundamental units of nuclear or chromosome architecture [C. Meng and R. Berezney (1991) J. Cell Biol. 115, 95a; E. Sparvoli et al. (1994) J. Cell Sci. 10...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1998.4311

    authors： Zink D,Bornfleth H,Visser A,Cremer C,Cremer T

    更新日期：1999-02-25 00:00:00

  • C-banding of Peromyscus constitutive heterochromatin persists following histone hyperacetylation.

    abstract：:Approx. 35% of the DNA of cultured cells from the cactus mouse, Peromuscus eremicus, is contained in highly condensed constitutive heterochromatin which can be visualized in metaphase chromosomes stained by the C-band technique. Previous studies have shown this constitutive heterochromatin to contain a large proportio...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(83)90209-4

    authors： Halleck MS,Schlegel RA

    更新日期：1983-09-01 00:00:00

  • Specific binding of [3H]heparin to human carcinoma SW-13 and other mammalian cells.

    abstract：:This study reports on the specific binding of [3H]heparin to human adrenocortical carcinoma cell line SW-13. Heparin binding to SW-13 cells is specific, saturable, and time- and temperature-dependent with maximum binding occurring between 90 and 120 min at 22 degrees C. Scatchard analysis revealed two classes of bindi...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(90)90099-v

    authors： Halper J

    更新日期：1990-04-01 00:00:00

  • Fetal-adult phenotype transition, in terms of the serum dependency and growth factor requirements, of human skin fibroblast migration.

    abstract：:Since the migration of human fetal skin fibroblasts into a denuded area of a cell monolayer has been demonstrated to be serum-independent (fetal phenotype), whereas that of adult-donor skin fibroblasts is serum-dependent (adult phenotype), a study was carried out to determine at which life stage the fetal-adult phenot...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1995.1342

    authors： Kondo H,Yonezawa Y

    更新日期：1995-10-01 00:00:00

  • The ability of AIF-1 to activate human vascular smooth muscle cells is lost by mutations in the EF-hand calcium-binding region.

    abstract：:Allograft Inflammatory Factor-1 (AIF-1) is a cytoplasmic calcium-binding protein expressed in vascular smooth muscle cells (VSMC) in response to injury or cytokine stimulation. AIF-1 contains a partially conserved EF-hand calcium-binding domain, and participates in VSMC activation by activation of Rac1 and induction o...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2005.03.002

    authors： Autieri MV,Chen X

    更新日期：2005-07-01 00:00:00

  • Changes in deoxyribonucleoside triphosphate pools in the starfish oocyte during maturation and early embryogenesis.

    abstract：:dNTP pools are quite low in immature oocytes of the starfish, expand during the 1-methyladenine-induced maturational process and thereafter reach a maximal level (approx. 35, 20, 15 and 5 fmoles/egg for dTTP, dCTP, dATP and dGTP, respectively) which is maintained in overmatured eggs. Maturing oocytes were inseminated ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/s0014-4827(83)80023-8

    authors： Nagano H,Okano K,Ikegami S

    更新日期：1983-04-15 00:00:00

  • In chicken leukemia cells globin genes are fully transcribed but their rnas are retained in the perinucleolar area.

    abstract：:Using hybridization in situ with a ribo-probe recognizing transcripts of the chicken alpha A globin gene, we show here that in proliferating AEV-transformed erythroblasts this gene is strongly transcribed, but the corresponding transcripts are retained in the nuclei. Most surprisingly, this globin RNA accumulates in t...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2001.5332

    authors： Iarovaia O,Razin SV,Linares-Cruz G,Sjakste N,Scherrer K

    更新日期：2001-11-01 00:00:00

  • Carbonic anhydrase II plays a major role in osteoclast differentiation and bone resorption by effecting the steady state intracellular pH and Ca2+.

    abstract：:Carbonic anhydrase II (CA II) expression in characteristic for the early stage of osteoclast differentiation. To study how CA II, which is crucial in proton generation in mature osteoclasts, influences the osteoclast differentiation process we performed rat bone marrow cultures. In this model, acetazolamide, a specifi...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1998.4071

    authors： Lehenkari P,Hentunen TA,Laitala-Leinonen T,Tuukkanen J,Väänänen HK

    更新日期：1998-07-10 00:00:00

  • Tumor exosomes induce tunneling nanotubes in lipid raft-enriched regions of human mesothelioma cells.

    abstract：:Tunneling nanotubes (TnTs) are long, non-adherent, actin-based cellular extensions that act as conduits for transport of cellular cargo between connected cells. The mechanisms of nanotube formation and the effects of the tumor microenvironment and cellular signals on TnT formation are unknown. In the present study, we...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2014.01.014

    authors： Thayanithy V,Babatunde V,Dickson EL,Wong P,Oh S,Ke X,Barlas A,Fujisawa S,Romin Y,Moreira AL,Downey RJ,Steer CJ,Subramanian S,Manova-Todorova K,Moore MAS,Lou E

    更新日期：2014-04-15 00:00:00

  • Polycystins and intercellular mechanotransduction: A precise dosage of polycystin 2 is necessary for alpha-actinin reinforcement of junctions upon mechanical stimulation.

    abstract：:Polycystins 1 and 2, which are mutated in Autosomal Polycystic Kidney Disease, are involved in mechanotransduction through various mechanisms. In renal cells, polycystins not only have an important mechanotransductive role in primary cilia but are also present in intercellular contacts but their role there remains unc...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2016.08.021

    authors： Bhoonderowa L,Hameurlaine F,Arbabian A,Faqir F,Amblard F,Coscoy S

    更新日期：2016-10-15 00:00:00

  • Microtubule-mediated NF-kappaB activation in the TNF-alpha signaling pathway.

    abstract：:The microtubule cytoskeleton is known to play a role in cell structure and serve as a scaffold for a variety of active molecules in processes as diverse as motility and cell division. The literature on the role of microtubules in signal transduction, however, is marked by inconsistencies. We have investigated a well-s...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2009.08.020

    authors： Jackman RW,Rhoads MG,Cornwell E,Kandarian SC

    更新日期：2009-11-15 00:00:00

  • Regulation of microphthalmia-associated transcription factor MITF protein levels by association with the ubiquitin-conjugating enzyme hUBC9.

    abstract：:The basic helix-loop-helix/leucine zipper (bHLH/ZIP) microphthalmia-associated transcription factor (MITF) regulates transcription of genes encoding enzymes essential for melanin biosynthesis in melanocytes and retinal pigmented epithelial cells. To determine how MITF activity is regulated, we used the yeast two-hybri...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2000.4803

    authors： Xu W,Gong L,Haddad MM,Bischof O,Campisi J,Yeh ET,Medrano EE

    更新日期：2000-03-15 00:00:00

  • Resistance to DNA denaturation in irradiated Chinese hamster V79 fibroblasts is linked to cell shape.

    abstract：:Exponentially growing Chinese hamster V79-171b lung fibroblasts seeded at high density on plastic (approximately 7 x 10(3) cells/cm2) flatten, elongate, and produce significant amounts of extracellular fibronectin. When lysed in weak alkali/high salt, the rate of DNA denaturation following exposure to ionizing radiati...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(91)90105-4

    authors： Olive PL,Vanderbyl S,MacPhail SH

    更新日期：1991-04-01 00:00:00

  • Unidirectional co-stimulation by a non-mating strain of Tetrahymena thermophila.

    abstract：:We report that a fatty acid auxotroph of Tetrahymena thermophila (RH179E1) fails to mate, yet retains the ability to co-stimulate normal cells unidirectionally. Thus, co-stimulation can be analyzed experimentally in the absence of pair formation. We show that the co-stimulation of normal cells of one mating type is su...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(87)90225-4

    authors： Katz M,Conner RL,Baum MP,Orias E

    更新日期：1987-03-01 00:00:00

  • Characterization of the intermediate filament proteins of murine mammary gland epithelial cells. Response to collagen substratum.

    abstract：:The insoluble cytoskeletal material remaining after detergent lysis of 'Normal' Murine Mammary Gland (NMuMG) cells, growing on plastic or collagen gel substrata, was analyzed by two-dimensional gel electrophoresis. The identity of the cytoskeletal elements was determined by their solubility properties, electrophoretic...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(86)90343-5

    authors： Hall HG,Bissell MJ

    更新日期：1986-02-01 00:00:00

  • Establishment and characterization of hybrid rat mast cells.

    abstract：:Rat peritoneal mast cells (RPMC) and rat basophilic leukemia (RBL) cells are representative of connective tissue-type (CTMC) and mucosal-type (MMC) mast cells, respectively. Using polyethylene glycol, we have fused RPMC with 6-thioguanine resistant, HAT (hypoxanthine, aminopterin, thymidine) sensitive RBL-CA10.7 or RB...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(91)90369-6

    authors： Zheng YL,Chan BM,Rector ES,Berczi I,Froese A

    更新日期：1991-06-01 00:00:00

  • Phosphorylation of filamin A by Cdk1 regulates filamin A localization and daughter cell separation.

    abstract：:In cell culture, many adherent mammalian cells undergo substantial actin cytoskeleton rearrangement prior to mitosis as they detach from the extracellular matrix and become spherical. At the end of mitosis, the actin cytoskeleton is required for cytokinesis and the reassembly of interphase structures as cells spread a...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2014.10.024

    authors： Szeto SGY,Williams EC,Rudner AD,Lee JM

    更新日期：2015-01-15 00:00:00

  • Induction of human high K(M) 5'-nucleotidase in cultured 293 cells.

    abstract：:Human 293 cells were stably transfected with a plasmid introducing a receptor for the ecdysone analog muristerone. The cells were further stably transfected with muristerone-inducible expression vectors carrying either the cDNA for the human high K(M) 5'-nucleotidase or the coding sequence of the nucleotidase linked t...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1999.4681

    authors： Gazziola C,Moras M,Ferraro P,Gallinaro L,Verin R,Rampazzo C,Reichard P,Bianchi V

    更新日期：1999-12-15 00:00:00

  • Regulation of subcellular localization of the antiproliferative protein Tob by its nuclear export signal and bipartite nuclear localization signal sequences.

    abstract：:Tob, a member of the Tob and BTG antiproliferative protein family, plays an important role in many cellular processes including cell proliferation. In this study, we have addressed molecular mechanisms regulating subcellular localization of Tob. Treatment with leptomycin B, an inhibitor of nuclear export signal (NES) ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2003.12.016

    authors： Maekawa M,Yamamoto T,Nishida E

    更新日期：2004-04-15 00:00:00

  • Intronic miR-932 targets the coding region of its host gene, Drosophila neuroligin2.

    abstract：:Despite great progress for two decades in microRNAs (miRNAs), the direct regulation of host gene by intragenic (mostly intronic) miRNA is conceptually plausible but evidence-limited. Here, we report that intronic miR-932 could target its host gene via binding with coding sequence (CDS) region rather than regular 3'UTR...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2016.01.017

    authors： Qian J,Tu R,Yuan L,Xie W

    更新日期：2016-06-10 00:00:00

  • Protein kinase C regulates the phosphorylation and oligomerization of ERM binding phosphoprotein 50.

    abstract：:Ezrin-Radixin-Moesin (ERM) binding phosphoprotein 50 (EBP50, a.k.a. NHERF-1) is a scaffold protein essential for the localization and coordinated activity of apical transporters, enzymes and receptors in epithelial cells. EBP50 acts via multiple protein binding interactions, including oligomerization through interacti...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2005.02.011

    authors： Fouassier L,Nichols MT,Gidey E,McWilliams RR,Robin H,Finnigan C,Howell KE,Housset C,Doctor RB

    更新日期：2005-05-15 00:00:00

  • Multiple proteases are involved in thymocyte apoptosis.

    abstract：:To investigate the involvement of proteases in apoptosis, rat thymocytes were treated with the glucocorticoid hormone methylprednisolone or the topoisomerase II inhibitor etoposide in the presence of selective substrate inhibitors of either interleukin-1 beta-converting enzyme (ICE), (Z-Val-Ala-Asp-chloromethylketone,...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1995.1391

    authors： Zhivotovsky B,Gahm A,Ankarcrona M,Nicotera P,Orrenius S

    更新日期：1995-12-01 00:00:00