当前位置： SCI文献检索 > EXPERIMENTAL CELL RESEARCH期刊下所有文献 > Phosphorylation of filamin A by Cdk1 regulates filamin A localization and daughter cell separation.

Phosphorylation of filamin A by Cdk1 regulates filamin A localization and daughter cell separation.

Abstract：

:In cell culture, many adherent mammalian cells undergo substantial actin cytoskeleton rearrangement prior to mitosis as they detach from the extracellular matrix and become spherical. At the end of mitosis, the actin cytoskeleton is required for cytokinesis and the reassembly of interphase structures as cells spread and reattach to substrate. To understand the processes regulating mitotic cytoskeletal remodeling, we studied how mitotic phosphorylation regulates filamin A (FLNa). FLNa is an actin-crosslinking protein that was previously identified as a cyclin-dependent kinase 1 (Cdk1) binding partner and substrate in vitro. Using quantitative label-based mass spectrometry, we find that FLNa serines 1084, 1459 and 1533 are phosphorylated in mitotic HeLa cells and all three sites match the phosphorylation consensus sequence of Cdk1. To investigate the functional role of mitotic FLNa phosphorylation, we mutated serines 1084, 1459 and 1533 to nonphosphorylatable alanine residues and expressed GFP-tagged FLNa(S1084A,S1459A,S1533A) (FLNa-AAA GFP) in a FLNa-deficient human melanoma cell line called M2. M2 cells expressing FLNa-AAA GFP have enhanced FLNa-AAA GFP and actin localization at sites of contact between daughter cells, impaired post-mitotic daughter cell separation and defects in cell migration. Therefore, mitotic phosphorylation of FLNa is important for successful cell division and interphase cell behavior.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Szeto SGY,Williams EC,Rudner AD,Lee JM

doi

10.1016/j.yexcr.2014.10.024

subject

Has Abstract

pub_date

2015-01-15 00:00:00

pages

248-266

issue

2

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(14)00491-1

journal_volume

330

pub_type

杂志文章

相关文献

EXPERIMENTAL CELL RESEARCH文献大全
  • Induction of M-phase entry of prophase-blocked mouse oocytes through microinjection of okadaic acid, a specific phosphatase inhibitor.

    abstract：:We report that a specific inhibitor of types 1 and 2A phosphatases, okadaic acid (OA), induces germinal vesicle break down (GVBD) and chromosome condensation when microinjected into denuded mouse oocytes maintained in prophase block by analogs of cAMP, inhibitors of phosphodiesterase, or a tumor-promoting phorbol este...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(91)90159-r

    authors： Gavin AC,Tsukitani Y,Schorderet-Slatkine S

    更新日期：1991-01-01 00:00:00

  • Autoimmune regulator is acetylated by transcription coactivator CBP/p300.

    abstract：:The Autoimmune Regulator (AIRE) is a regulator of transcription in the thymic medulla, where it controls the expression of a large set of peripheral-tissue specific genes. AIRE interacts with the transcriptional coactivator and acetyltransferase CBP and synergistically cooperates with it in transcriptional activation....

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2012.04.013

    authors： Saare M,Rebane A,Rajashekar B,Vilo J,Peterson P

    更新日期：2012-08-15 00:00:00

  • Differential activation of cyclin and cyclin-dependent kinase genes by adenovirus E1A12S cDNA product.

    abstract：:The differential activation of cyclin and cyclin-dependent kinase genes by the adenovirus E1A gene product (E1A) or serum factors was studied with a rat 3Y1 derivative cell line, g12-21, in which the E1A12S cDNA can be expressed in response to dexamethasone (dex). The induction of DNA synthesis in quiescent g12-21 cel...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1993.1262

    authors： Ishii T,Shimizu M,Kanayama Y,Nakada S,Nojima H,Oda K

    更新日期：1993-10-01 00:00:00

  • Endothelial nitric oxide synthase is segregated from caveolin-1 and localizes to the leading edge of migrating cells.

    abstract：:The enzyme endothelial Nitric Oxide Synthase (eNOS) is involved in key physiological and pathological processes, including cell motility and apoptosis. It is widely believed that at the cell surface eNOS is localized in caveolae, where caveolin-1 negatively regulates its activity, however, there are still uncertaintie...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2005.12.014

    authors： Bulotta S,Cerullo A,Barsacchi R,De Palma C,Rotiroti D,Clementi E,Borgese N

    更新日期：2006-04-01 00:00:00

  • Microinjected anti-actin antibodies decrease gap junctional intercellular commmunication in cultured astrocytes.

    abstract：:The purpose of the present study was to investigate the influence of the actin cytoskeleton on gap junctional intercellular communication (GJIC) in cultured astrocytes. This report describes an decrease of GJIC following microinjection of anti-actin antibodies or cytochalasin D treatment. Dye transfer of microinjected...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2002.5652

    authors： Theiss C,Meller K

    更新日期：2002-12-10 00:00:00

  • Oocyte-specific deletion of Gsα induces oxidative stress and deteriorates oocyte quality in mice.

    abstract：:The stimulatory heterotrimeric Gs protein alpha subunit (Gsα) is a ubiquitous guanine nucleotide-binding protein that regulates the intracellular cAMP signaling pathway and consequently participates in a wide range of biological events. In the reproductive system, despite Gsα being associated with oocyte meiotic arres...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2018.07.023

    authors： Xie Y,Wu B,Jin Y,Zhang A,Sun X,Zhang X,Gao X,Dong R,Li H,Gao J

    更新日期：2018-09-15 00:00:00

  • Focal adhesion kinase regulates cell-cell contact formation in epithelial cells via modulation of Rho.

    abstract：:Focal Adhesion Kinase (FAK) is a non-receptor tyrosine kinase that plays a key role in cellular processes such as cell adhesion, migration, proliferation and survival. Recent studies have also implicated FAK in the regulation of cell-cell adhesion. Here, evidence is presented showing that siRNA-mediated suppression of...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2008.08.010

    authors： Playford MP,Vadali K,Cai X,Burridge K,Schaller MD

    更新日期：2008-10-15 00:00:00

  • Caloric restriction: conservation of cellular replicative capacity in vitro accompanies life-span extension in mice.

    abstract：:We have tested whether life-long caloric restriction (CR) slows or delays the age-related loss of cellular replicative potential that occurs during normal aging in ad libitum (AL) fed mice. Both mean and maximum life spans of the restricted animals (60% of AL intake) were significantly extended 30-40% by CR treatment....

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1995.1091

    authors： Pendergrass WR,Li Y,Jiang D,Fei RG,Wolf NS

    更新日期：1995-04-01 00:00:00

  • Specific binding of [3H]heparin to human carcinoma SW-13 and other mammalian cells.

    abstract：:This study reports on the specific binding of [3H]heparin to human adrenocortical carcinoma cell line SW-13. Heparin binding to SW-13 cells is specific, saturable, and time- and temperature-dependent with maximum binding occurring between 90 and 120 min at 22 degrees C. Scatchard analysis revealed two classes of bindi...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(90)90099-v

    authors： Halper J

    更新日期：1990-04-01 00:00:00

  • The IGF-1-IGF-1 receptor system modulates myocyte proliferation but not myocyte cellular hypertrophy in vitro.

    abstract：:In preliminary experiments it was established that the hypertrophic and hyperplastic responses of neonatal cardiac myocytes in culture were associated with enhanced expression of IGF-1 and IGF-1 receptors in these cells. Therefore, to determine the role of IGF-1 receptors on myocyte growth, cells were exposed to antis...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1994.1343

    authors： Kajstura J,Cheng W,Reiss K,Anversa P

    更新日期：1994-12-01 00:00:00

  • NLRP3 inflammasome inhibition attenuates cisplatin-induced renal fibrosis by decreasing oxidative stress and inflammation.

    abstract：BACKGROUND/AIMS:The NOD-like receptor, pyrin domain containing-3 (NLRP3) inflammasome is involved in the progression of chronic kidney disease in several rodent models. Here, we investigated whether a specific inhibitor of NLRP3 inflammasome, MCC950, can attenuate cisplatin-induced renal fibrosis. MATERIALS:Renal fibr...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2019.07.001

    authors： Li S,Lin Q,Shao X,Mou S,Gu L,Wang L,Zhang Z,Shen J,Zhou Y,Qi C,Jin H,Pang H,Ni Z

    更新日期：2019-10-01 00:00:00

  • Isoforms of p38MAPK gamma and delta contribute to differentiation of human AML cells induced by 1,25-dihydroxyvitamin D₃.

    abstract：:Inhibition of p38MAPK alpha/beta is known to enhance 1,25-dihydroxyvitamin (1,25D)-induced monocytic differentiation, but the detailed mechanism of this effect was not clear. We now show that the enhancement of differentiation becomes apparent with slow kinetics (12-24 h). Interestingly, the inhibition of p38MAPK alph...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2010.08.010

    authors： Zhang J,Harrison JS,Studzinski GP

    更新日期：2011-01-01 00:00:00

  • p66Shc promotes HCC progression in the tumor microenvironment via STAT3 signaling.

    abstract：:The development of hepatocellular carcinoma (HCC) is strongly associated with chronic inflammation. p66Shc is an oxidase previously shown to promote androgen-independent cell growth through generation of reactive oxygen species. However, the importance and biologic functions of p66Shc in HCC are unclear. The clinical ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2019.111550

    authors： Huang P,Feng X,Zhao Z,Yang B,Fang T,Guo M,Xia J

    更新日期：2019-10-15 00:00:00

  • TGF-beta receptors, in a Smad-independent manner, are required for terminal skeletal muscle differentiation.

    abstract：:Skeletal muscle differentiation is strongly inhibited by transforming growth factor type beta (TGF-beta), although muscle formation as well as regeneration normally occurs in an environment rich in this growth factor. In this study, we evaluated the role of intracellular regulatory Smads proteins as well as TGF-beta-r...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2010.04.031

    authors： Droguett R,Cabello-Verrugio C,Santander C,Brandan E

    更新日期：2010-09-10 00:00:00

  • Strong association of bovine IgM with microvilli and their microfilament core from 13762 ascites tumor cells.

    abstract：:Microfilament cores, obtained by extracting 13762 mammary ascites tumor cell microvilli with Triton X-100, contain a major glycoprotein migrating at an apparent molecular weight of 80 kDa by dodecyl sulfate-polyacrylamide gel electrophoresis. The 80-kDa component is a disulfide-linked multimer, as demonstrated by velo...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(89)90288-7

    authors： Ye XH,Metcalf TN 3rd,Andrews DM,Carraway CA,Carraway KL

    更新日期：1989-05-01 00:00:00

  • Simvastatin induces differentiation of rabbit articular chondrocytes via the ERK-1/2 and p38 kinase pathways.

    abstract：:Statins are competitive inhibitors of hydroxy-methyl-glutaryl Coenzyme A (HMG-CoA) reductase, a key enzyme involved in the conversion of HMG-CoA to the cholesterol precursor mevalonate. Some statins, such as simvastatin (simvastatin), have been shown to have anti-cancer and anti-inflammatory effects, reducing cartilag...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2016.07.025

    authors： Han Y,Kim SJ

    更新日期：2016-08-15 00:00:00

  • An investigation of adhesion and detachment in slime mould amoebae using columns of hydrophobic beads.

    abstract：:Vegetative amoebae of Dictyostelium discoideum (strain AX2) adhere to columns of hydrophobic beads (Bio-Beads SM-2) which have been coated with axenic growth medium. This binding is blocked by sodium azide and other inhibitors of aerobic respiration. Bound cells are not detached by lowering the temperature but they ca...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(84)90235-0

    authors： Glynn PJ,Clarke KR

    更新日期：1984-05-01 00:00:00

  • Cylindrical substratum induces different patterns of actin microfilament bundles in nontransformed and in ras-transformed epitheliocytes.

    abstract：:The influence of the cylindrical substratum surface on the actin microfilament bundle and the extracellular matrix (fibronectin and laminin) patterns in nontransformed epithelial cells of the IAR-2 rat line and in their N-ras-transformed descendants, IAR-ras-c4 cells, was studied. The cells were cultured on substrata ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1996.0354

    authors： Levina EM,Domnina LV,Rovensky YA,Vasiliev JM

    更新日期：1996-11-25 00:00:00

  • p21ras. Heterogeneous localization in transformed cells.

    abstract：:The cellular targets for the Kirsten murine sarcoma virus (KiMSV)-transforming protein, p21ras, are unknown. Other studies have indicated that the mature form of p21 is distributed diffusely on the cytoplasmic face of the plasma membrane. However, after fixation without buffer washes, indirect immunofluorescent staini...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/s0014-4827(85)80017-3

    authors： Myrdal SE,Auersperg N

    更新日期：1985-08-01 00:00:00

  • Differentiation of C2C12 myoblasts expressing lamin A mutated at a site responsible for Emery-Dreifuss muscular dystrophy is improved by inhibition of the MEK-ERK pathway and stimulation of the PI3-kinase pathway.

    abstract：:Mutation R453W in A-type lamins, that are major nuclear envelope proteins, generates Emery-Dreifuss muscular dystrophy. We previously showed that mouse myoblasts expressing R453W-lamin A incompletely exit the cell cycle and differentiate into myocytes with a low level of multinucleation. Here we attempted to improve d...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2008.01.018

    authors： Favreau C,Delbarre E,Courvalin JC,Buendia B

    更新日期：2008-04-01 00:00:00

  • A novel 3D high-content assay identifies compounds that prevent fibroblast invasion into tissue surrogates.

    abstract：:Invasion processes underlie or accompany several pathological processes but only a limited number of high-throughput capable phenotypic models exist to test anti-invasive compounds in vitro. We here evaluated 3D co-cultures as a high-content phenotypic screening system for fibrotic invasive processes. 3D multicellular...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2015.10.003

    authors： Wenzel C,Otto S,Prechtl S,Parczyk K,Steigemann P

    更新日期：2015-11-15 00:00:00

  • Characterization of the C-terminal ER membrane anchor of PTP1B.

    abstract：:The tyrosine phosphatase PTP1B is an important regulator of cell function. In living cells PTP1B activity is restricted to the vicinity of the endoplasmic reticulum (ER) by post-translational C-terminal attachment of PTP1B to the ER membrane network. In our study we investigated the membrane anchor of PTP1B by use of ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2007.05.025

    authors： Anderie I,Schulz I,Schmid A

    更新日期：2007-09-10 00:00:00

  • Intracellular localization of secretable cAMP in relaying Dictyostelium discoideum cells.

    abstract：:Dictyostelium discoideum cells synthesize and secrete the chemoattractant cAMP within minutes after chemotactic stimulation. During development, this signal-relay process is instrumental in cell aggregation, pattern formation, and differentiation. Cyclic AMP is known to accumulate inside the cell before secretion. In ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(89)90181-x

    authors： Schoen CD,Arents JC,Bruin T,Van Driel R

    更新日期：1989-03-01 00:00:00

  • Intranuclear distribution of SV40 large T-antigen and transformation-related protein p53 in abortively infected cells.

    abstract：:The intranuclear localization of SV40 T-antigen (T-Ag) and the cellular protein p53 was studied in SV40 abortively infected baby mouse kidney cells using two complementary methods of ultrastructural immunocytochemistry in combination with preferential staining of nuclear RNP components and electron microscope autoradi...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(88)90026-2

    authors： Puvion E,Duthu A,Harper F,Ehrhart JC,Viron A,May P

    更新日期：1988-07-01 00:00:00

  • Differential regulation of protooncogenes c-jun and jun D expressions by protein tyrosine kinase, protein kinase C, and cyclic-AMP mitogenic pathways in dog primary thyrocytes: TSH and cyclic-AMP induce proliferation but downregulate C-jun expression.

    abstract：:The expressions of the protooncogenes c-jun and jun D have been investigated in dog thyrocytes in a primary culture whose proliferation is stimulated by three distinct intracellular signaling pathways (1) the thyrotropin (TSH) or forskolin-cyclic-AMP-mediated cascade; (2) the protein kinase C pathway activated by diac...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(91)90253-q

    authors： Reuse S,Pirson I,Dumont JE

    更新日期：1991-10-01 00:00:00

  • High glucose increases glomerular filtration barrier permeability by activating protein kinase G type Iα subunits in a Nox4-dependent manner.

    abstract：:Hyperglycemia is a primary factor that disturbs podocyte function in the glomerular filtration process; this disturbance leads to the development of diabetic nephropathy, and ultimately, renal failure. Podocyte function may also be altered by biological agents that modify protein kinase activity, including the cGMP-ac...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2013.09.005

    authors： Piwkowska A,Rogacka D,Audzeyenka I,Angielski S,Jankowski M

    更新日期：2014-01-01 00:00:00

  • The small molecule genkwanine M induces single mode, mesenchymal tumor cell motility.

    abstract：:Individual tumor cells utilize one of two modes of motility to invade the extracellular matrix, mesenchymal or amoeboid. We have determined that the diterpenoid genkwanine M (GENK) enhances the mesenchymal mode of cell motility that is intrinsic to HT-1080 osteosarcoma cells, stimulates a mesenchymal mode of motility ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2013.01.005

    authors： Zimmerman C,Austin P,Khong A,McLeod S,Bean B,Forestieri R,Andersen RJ,Jan E,Roberge M,Roskelley CD

    更新日期：2013-04-01 00:00:00

  • Multiple proteases are involved in thymocyte apoptosis.

    abstract：:To investigate the involvement of proteases in apoptosis, rat thymocytes were treated with the glucocorticoid hormone methylprednisolone or the topoisomerase II inhibitor etoposide in the presence of selective substrate inhibitors of either interleukin-1 beta-converting enzyme (ICE), (Z-Val-Ala-Asp-chloromethylketone,...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1995.1391

    authors： Zhivotovsky B,Gahm A,Ankarcrona M,Nicotera P,Orrenius S

    更新日期：1995-12-01 00:00:00

  • Human dermal fibroblasts escape from the long-term phenocopy of senescence induced by psoralen photoactivation.

    abstract：:We have previously shown that following psoralen photoactivation (PUVA treatment) human dermal fibroblasts undergo long-term growth arrest as well as morphological and functional changes reminiscent of cellular senescence [ 1 ]. In the absence of molecular data on what constitutes normal senescence, it has been diffic...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2002.5476

    authors： Ma W,Wlaschek M,Brenneisen P,Schneider LA,Hommel C,Hellweg C,Sauer H,Wartenberg M,Herrmann G,Meewes C,Boukamp P,Scharffetter-Kochanek K

    更新日期：2002-04-01 00:00:00

  • Acceleration of cytokinesis in PtK1 cells treated with microtubule inhibitors.

    abstract：:Mitotic PtK1 cells were treated at furrow initiation with microtubule poisons to determine the role of microtubules in the regulation of terminal cytokinetic events. The administration of anti-microtubule agents in late anaphase accelerated the rate of cytokinesis by approx. 60% as measured by changes in furrow diamet...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(83)90413-5

    authors： Hamilton BT,Snyder JA

    更新日期：1983-04-01 00:00:00