Abstract：
:Polycystins 1 and 2, which are mutated in Autosomal Polycystic Kidney Disease, are involved in mechanotransduction through various mechanisms. In renal cells, polycystins not only have an important mechanotransductive role in primary cilia but are also present in intercellular contacts but their role there remains unclear. Here, we address the hypothesis that polycystins are involved in mechanotransduction via intercellular junctions, which would be expected to have consequences on tissue organization. We focused on the role of polycystin 2, which could be involved in mechanical organization at junctions either by its channel activity or by the direct recruitment of cytoskeleton components such as the F-actin cross-linker α-actinin. After mechanical stimulation of intercellular junctions in MDCK renal epithelial cells, α-actinin is rapidly recruited but this is inhibited upon overexpression of PC2 or the D509V mutant that lacks channel activity, and is also decreased upon PC2 silencing. This suggests that a precise dosage of PC2 is necessary for an adequate mechanosensitive α-actinin recruitment at junctions. At the multicellular level, a change in PC2 expression was associated with changes in velocity in confluent epithelia and during wound healing together with a loss of orientation. This study suggests that the mechanosensitive regulation of cytoskeleton by polycystins in intercellular contacts may be important in the context of ADPKD.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Bhoonderowa L,Hameurlaine F,Arbabian A,Faqir F,Amblard F,Coscoy Sdoi
10.1016/j.yexcr.2016.08.021subject
Has Abstractpub_date
2016-10-15 00:00:00pages
23-35issue
1eissn
0014-4827issn
1090-2422pii
S0014-4827(16)30260-9journal_volume
348pub_type
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journal_title：Experimental cell research
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pub_type： 杂志文章,评审
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更新日期：2017-07-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(88)90281-9
更新日期：1988-12-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0014-4827(88)90019-5
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pub_type： 杂志文章
doi：10.1016/j.yexcr.2005.12.032
更新日期：2006-04-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2016.02.014
更新日期：2016-03-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2014.10.015
更新日期：2015-02-15 00:00:00
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doi：10.1016/j.yexcr.2011.08.006
更新日期：2011-10-15 00:00:00
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更新日期：2010-02-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章,评审
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journal_title：Experimental cell research
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更新日期：2009-04-15 00:00:00
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journal_title：Experimental cell research
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更新日期：2011-10-01 00:00:00
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journal_title：Experimental cell research
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：1995-08-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2004.04.043
更新日期：2004-08-15 00:00:00
