Abstract：
:An attempt was made to determine the role of metabolic acidification of pericellular medium in regulating cell proliferation. A method of measuring the pH of pericellular medium at a distance exceeding a Debye radius of 5-10 A from the cell surface (pHp) was developed. The values of pHp and pH measured in the medium at a distance of greater than 1 cm from cells (pHm) were found to differ, depending on the cell population density. At a density of at least 7 X 10(5) cells/cm2 (maximum saturation density) and at pHm 7.4-7.6, pHp reached a value of approximately 6.5. It was found that pHm 6.5 was unfavorable for cell proliferation in sparse cultures, where pHm and pHp were equal. Based on these findings, low pHp as revealed in the present work using dense cultures at optimal pHm can be considered to be a limiting factor for cell multiplication.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Akatov VS,Lezhnev EI,Vexler AM,Kublik LNdoi
10.1016/0014-4827(85)90188-0subject
Has Abstractpub_date
1985-10-01 00:00:00pages
412-8issue
2eissn
0014-4827issn
1090-2422journal_volume
160pub_type
杂志文章abstract：:Dipeptidyl peptidase IV (DPPIV) is a serine peptidase that cleaves N-terminal dipeptides from polypeptides when the second residue is a proline or an alanine. We have recently cloned cDNAs for rat gp110, a membrane glycoprotein with Mr of 110,000 isolated initially from rat liver. Studies reported here establish that ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(89)90296-6
更新日期：1989-05-01 00:00:00
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journal_title：Experimental cell research
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2002.5540
更新日期：2002-07-01 00:00:00
abstract：:Phorbol esters have been reported to induce opposite responses in fetal myoblasts and in satellite cells isolated from adult skeletal muscles. We examined the possibility that different levels of protein kinase C (PKC) activity and different phorbol ester binding characteristics account for these responses. For this p...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(89)90420-5
更新日期：1989-07-01 00:00:00
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journal_title：Experimental cell research
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2012.05.013
更新日期：2012-08-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2001.5352
更新日期：2001-11-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1996.0344
更新日期：1996-11-25 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：1986-06-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2006.05.019
更新日期：2006-10-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1996.0223
更新日期：1996-07-10 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(91)90430-3
更新日期：1991-12-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2001.5331
更新日期：2001-10-01 00:00:00
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pub_type： 杂志文章,评审
doi：10.1016/j.yexcr.2017.03.034
更新日期：2017-07-15 00:00:00
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doi：10.1016/j.yexcr.2008.10.012
更新日期：2009-01-01 00:00:00
abstract：:The growth of malignant melanoma cells is inhibited by 12-O-tetradecanoylphorbol-13-acetate (TPA) while the growth of normal melanocytes is stimulated. We previously demonstrated that TPA inhibits the growth of Demel melanoma cells and leads to arrest at both at the G1/S and G2/M cell cycle transitions. To investigate...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1995.1356
更新日期：1995-11-01 00:00:00
abstract：:The segregation of the chick-specific HPRT gene was studied in three Chinese hamster-chick red blood cell hybrid lines. The three lines showed individual segregation kinetics, the segregation taking place in an exponential-like fashion. Bromodeoxyuridine becomes incorporated into the nuclear DNA and increases the spon...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(78)90374-9
更新日期：1978-05-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1995.1091
更新日期：1995-04-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1997.3667
更新日期：1997-08-25 00:00:00
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pub_type： 杂志文章
doi：10.1016/j.yexcr.2016.02.014
更新日期：2016-03-01 00:00:00
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doi：10.1016/j.yexcr.2003.10.003
更新日期：2004-02-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章,评审
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更新日期：2018-06-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章,评审
doi：10.1006/excr.1993.1022
更新日期：1993-02-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2014.11.011
更新日期：2015-01-15 00:00:00
abstract：:Subsets of glycoproteins are thought to require lectin-like membrane receptors for efficient export out of the endoplasmic reticulum (ER). To identify new members related to two previously characterized intracellular lectins ERGIC-53/p58 and VIP36, we carried out an extensive database search using the conserved carboh...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/s0014-4827(03)00161-7
更新日期：2003-08-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(92)90028-7
更新日期：1992-12-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1994.1333
更新日期：1994-11-01 00:00:00
abstract：:The precise distribution of DNA within the resting human T lymphocyte nucleolus has been investigated, at the ultrastructural level, by cytochemical and molecular immunocytochemical techniques. The nucleolus is partially enveloped by a layer of condensed chromatin which, at several places, penetrates into the nucleola...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1993.1110
更新日期：1993-04-01 00:00:00
