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Establishment of a germ-line competent C57BL/6 embryonic stem cell line.

Abstract：

:Embryonic stem (ES) cell lines have been derived from blastocysts of the inbred mouse strain C57BL/6. The highest frequencies of ES cell colonies were observed when blastocysts were explanted directly onto growth-arrested feeder layers of 5637 human bladder carcinoma cells in the presence of conditioned medium. One of the male ES cell lines tested (BL/6-III) was shown to be karyotypically stable and germ-line competent when introduced into BALB/c host blastocysts. These results demonstrate that ES cell lines from inbred mouse strains other than 129/Sv may be used as vectors to introduce selected mutations into the germ-line of mice.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Ledermann B,Bürki K

doi

10.1016/0014-4827(91)90430-3

subject

Has Abstract

pub_date

1991-12-01 00:00:00

pages

254-8

issue

2

eissn

0014-4827

issn

1090-2422

journal_volume

197

pub_type

杂志文章

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