Variations in signal-mediated nuclear transport during the cell cycle in BALB/c 3T3 cells.

abstract：:Using hybridization in situ with a ribo-probe recognizing transcripts of the chicken alpha A globin gene, we show here that in proliferating AEV-transformed erythroblasts this gene is strongly transcribed, but the corresponding transcripts are retained in the nuclei. Most surprisingly, this globin RNA accumulates in t...

journal_title：Experimental cell research

authors： Iarovaia O,Razin SV,Linares-Cruz G,Sjakste N,Scherrer K

更新日期：2001-11-01 00:00:00

abstract：:Chronic lymphocytic leukemia lymphocytes were used to study mechanisms involved in apoptosis (programmed cell death). Apoptosis, which was determined by morphological changes including cell death and by internucleosomal DNA fragmentation, occurred during culture for 1 to 2 days in a portion of the cells from three of ...

journal_title：Experimental cell research

authors： Forbes IJ,Zalewski PD,Giannakis C,Cowled PA

更新日期：1992-02-01 00:00:00

abstract：:Skin extracellular matrix (ECM) molecules regulate a variety of cellular activities, including cell movement, which are central to wound healing and metastasis. Regulated cell movement is modulated by proteases and their associated molecules, including the serine proteases urinary-type plasminogen activator (uPA) and ...

journal_title：Experimental cell research

authors： Jones JM,Cohen RL,Chambers DA

更新日期：2002-11-01 00:00:00

abstract：:Regions rich in 5-methylcytosine were localized in male metaphase chromosomes of the Indian muntjac deer (Muntiakus muntjak). Chromosomes were ultraviolet irradiated and subsequently photooxidized in the presence of methylene blue to induce maximum DNA denaturation. Following treatment with anti 5-methylcytosine antib...

journal_title：Experimental cell research

authors： Vasilikaki-Baker H,Nishioka Y

更新日期：1983-08-01 00:00:00

abstract：:We have investigated the genetic stability of NIH-3T3 cells transfected with sequences coding for basic fibroblast growth factor (bFGF) by determining drug resistance and gene amplification potential. Colony-forming experiments and fluctuation analyses showed that the frequency and rate of resistance to N-(phosphonace...

journal_title：Experimental cell research

authors： Huang A,Jin H,Wright JA

更新日期：1994-08-01 00:00:00

abstract：:Myosin phosphatase target subunit 1 (MYPT1), together with catalytic subunit of type1 delta isoform (PP1cdelta) and a small 20-kDa regulatory unit (M20), form a heterotrimeric holoenzyme, myosin phosphatase (MP), which is responsible for regulating the extent of myosin light chain phosphorylation. Here we report the i...

journal_title：Experimental cell research

authors： Twomey E,Li Y,Lei J,Sodja C,Ribecco-Lutkiewicz M,Smith B,Fang H,Bani-Yaghoub M,McKinnell I,Sikorska M

更新日期：2010-01-01 00:00:00

abstract：:Laminin is a major component of the basal lamina upon which all epithelial cells reside in vivo. The synthesis of basal lamina components and their subsequent assembly into a morphologically distinct basal lamina is a differentiated function of epithelial cells in vivo. Ultrastructural studies in our laboratory show t...

journal_title：Experimental cell research

authors： Ecay TW,Valentich JD

更新日期：1992-11-01 00:00:00

abstract：:The male fetal lung begins to synthesize surfactant later in gestation than the female. This delay appears to be caused by androgens. We hypothesized that male fetal lung differentiation is delayed as a consequence of an extended phase of growth which is elicited by androgens. We observed that in vivo fetal lung prote...

journal_title：Experimental cell research

authors： Nielsen HC,Kirk WO,Sweezey N,Torday JS

更新日期：1990-05-01 00:00:00

abstract：:Exponentially growing Chinese hamster V79-171b lung fibroblasts seeded at high density on plastic (approximately 7 x 10(3) cells/cm2) flatten, elongate, and produce significant amounts of extracellular fibronectin. When lysed in weak alkali/high salt, the rate of DNA denaturation following exposure to ionizing radiati...

journal_title：Experimental cell research

authors： Olive PL,Vanderbyl S,MacPhail SH

更新日期：1991-04-01 00:00:00

abstract：:Lithium exerts neuroprotective actions that involve the inhibition of glycogen synthase kinase-3beta (GSK-3beta). Otherwise, recent studies suggest that sustained GSK-3beta inhibition is a hallmark of tumorigenesis. In this context, the present study was undertaken to examine whether lithium modulated cancer cell sens...

journal_title：Experimental cell research

authors： Beurel E,Kornprobst M,Blivet-Van Eggelpoël MJ,Ruiz-Ruiz C,Cadoret A,Capeau J,Desbois-Mouthon C

更新日期：2004-11-01 00:00:00

abstract：:The expression patterns of the testis-specific and somatic forms of the pyruvate dehydrogenase (PDH) E1 alpha subunit genes were examined in adult mouse testis by in situ hybridization with specific cDNA probes and by immunostaining. A considerable increase in the mRNA level of the testis-specific PDH E1 alpha gene wa...

journal_title：Experimental cell research

authors： Takakubo F,Dahl HH

更新日期：1992-03-01 00:00:00

abstract：:The role of p53 in transcriptional activation of genes involved in cell cycle progression is well established. However, the wide range of functions attributed to this gene suggests that some of them might be unrelated to transcription. Here we investigated p53 localization and recruitment to chromatin during Xenopus e...

journal_title：Experimental cell research

authors： Tchang F,Méchali M

更新日期：1999-08-25 00:00:00

abstract：:We present evidence for the possible involvement of both the RB and p53 proteins in the regulation of cellular senescence. Human fibroblasts immortalized with an inducible SV40 T-antigen become senescent following the de-induction of T-antigen. Plasmids expressing an alternative source of intact T-antigen restore prol...

journal_title：Experimental cell research

authors： Shay JW,Pereira-Smith OM,Wright WE

更新日期：1991-09-01 00:00:00

abstract：:Epithelial ovarian cancer (EOC) is thought to arise from the ovarian surface epithelium (OSE); however, the molecular events underlying this transformation are poorly understood. Germline mutations in the BRCA1 tumor suppressor gene result in a significantly increased risk of developing EOC and a large proportion of s...

journal_title：Experimental cell research

authors： Clark-Knowles KV,Garson K,Jonkers J,Vanderhyden BC

更新日期：2007-01-01 00:00:00

abstract：:Sertoli cells of testis belong to a unique type of polarized epithelial cells and are essential for spermatogenesis. They form the blood-testis barrier at the base of seminiferous tubule. Their numerous long, microtubule-rich processes extend inward and associate with developing germ cells to sustain germ cell growth ...

journal_title：Experimental cell research

authors： Wang F,Zhang Q,Cao J,Huang Q,Zhu X

更新日期：2008-01-01 00:00:00

abstract：:It has previously been shown by immunocytochemistry that the inactive X chromosome (Xi) in somatic cells of human and mouse females is marked by underacetylation of histone H4. It has been suggested that this may be important for transcriptional silencing of genes on Xi. We have now investigated X-inactivation in meio...

journal_title：Experimental cell research

authors： Armstrong SJ,Hultén MA,Keohane AM,Turner BM

更新日期：1997-02-01 00:00:00

abstract：:Cytostatic factor (CSF), found in the cytoplasm of unfertilized eggs of amphibians, causes metaphase arrest when microinjected into cleaving blastomeres. Although CSF from Rana pipiens eggs has been extracted and characterized, little is known about CSF extracted from eggs of other species. We investigated the conditi...

journal_title：Experimental cell research

authors： Moses RM,Masui Y

更新日期：1990-01-01 00:00:00

abstract：:Intermediate filaments (IFs), desmosomes, and their associates are built from multidomain proteins that form cytoskeletal scaffolds in the cytoplasm and the nucleus of vertebrate tissues. Mutations in more than 80 genes cause monogenic disorders that include severe skin fragility, myopathies, neurodegeneration, and pr...

journal_title：Experimental cell research

authors： Magin TM,Reichelt J,Hatzfeld M

更新日期：2004-11-15 00:00:00

abstract：:Transcription factors Mitf and NFATc1 share many downstream targets that are critical for osteoclastogenesis. Since RANKL signals induce/activate both NFATc1 and Mitf isoform-E (Mitf-E), a tissue-restricted Mitf isoform in osteoclasts, it is plausible that the two factors work together to promote osteoclastogenesis. A...

journal_title：Experimental cell research

authors： Lu SY,Li M,Lin YL

更新日期：2014-10-15 00:00:00

abstract：:Podosome formation in vascular smooth muscle cells is characterized by the recruitment of AFAP-110, p190RhoGAP, and cortactin, which have specific roles in Src activation, local down-regulation of RhoA activity, and actin polymerization, respectively. However, the molecular mechanism that underlies their specific recr...

journal_title：Experimental cell research

authors： Crimaldi L,Courtneidge SA,Gimona M

更新日期：2009-09-10 00:00:00

abstract：:RhoA activated kinases (ROCKs) are potent effectors of RhoA signaling for regulation of the cytoskeleton. ROCKs have been shown to be localized to several different subcellular locations, suggesting that its localization is context specific and regulated. However, the signaling mechanisms that control ROCK localizatio...

journal_title：Experimental cell research

authors： Kher SS,Worthylake RA

更新日期：2011-12-10 00:00:00

abstract：:Cell migration plays a major role during wound healing and is tightly controlled by a variety of growth factors and extracellular matrix proteins. The experiments reported here have been designed to study whether defined beta 1 integrins are involved in the platelet-derived growth-factor-AB (PDGF-AB)-modulated migrato...

journal_title：Experimental cell research

authors： Kirchberg K,Lange TS,Klein EC,Jungtäubl H,Heinen G,Meyer-Ingold W,Scharffetter-Kochanek K

更新日期：1995-09-01 00:00:00

abstract：:The segregation of the chick-specific HPRT gene was studied in three Chinese hamster-chick red blood cell hybrid lines. The three lines showed individual segregation kinetics, the segregation taking place in an exponential-like fashion. Bromodeoxyuridine becomes incorporated into the nuclear DNA and increases the spon...

journal_title：Experimental cell research

authors： Raskó I,Péter SL,Dallmann L,Bajszár G,Péter S,Burg K

更新日期：1978-05-01 00:00:00

abstract：:Interleukin-1 beta (IL-1) increases the synthesis of prostaglandins as well as the expression of synovial fluid phospholipase A2 (PLA2) mRNA and activity by chondrocytes. In order to examine the potential involvement of cyclooxygenase in the induction of prostaglandins by IL-1, the effect of IL-1 on rabbit articular c...

journal_title：Experimental cell research

authors： Lyons-Giordano B,Pratta MA,Galbraith W,Davis GL,Arner EC

更新日期：1993-05-01 00:00:00

abstract：:Dynamic subcellular localization is an important regulatory mechanism for many proteins. cIAP1 and cIAP2 are two closely related members of inhibitor of apoptosis (IAP) family that play a role both as caspase inhibitors and as mediators of tumor necrosis factor (TNF) receptor signaling. Here, we report that cIAP1 and ...

journal_title：Experimental cell research

authors： Vischioni B,Giaccone G,Span SW,Kruyt FA,Rodriguez JA

更新日期：2004-08-15 00:00:00

abstract：:A great deal of evidence suggests that long non-coding RNAs (lncRNAs) function in the tumorigenesis of retinoblastoma (RB). However, the roles of lncRNA ILF3-AS1 in RB are still unclear. In the present study, our work revealed that the lncRNA ILF3-AS1 was increased in both RB tissues and cell lines. Repression of ILF3...

journal_title：Experimental cell research

authors： Han S,Song L,Chen Y,Hou M,Wei X,Fan D

更新日期：2020-08-15 00:00:00

abstract：:Accumulating evidence indicates that elevated S100P promotes the pathogenesis of cancers, including colon cancer. S100P exerts its effects by binding to and activating the Receptor for Advance Glycation End-products (RAGE). The effects of up-regulated S100P/RAGE signaling on cell functions are well documented. Despite...

journal_title：Experimental cell research

authors： Onyeagucha BC,Mercado-Pimentel ME,Hutchison J,Flemington EK,Nelson MA

更新日期：2013-08-01 00:00:00

abstract：:To gain insight into the role of the endothelial cell during the pathophysiology of the angiogenic response, investigators have isolated micro- and macrovascular endothelial cells from a wide range of both animal and human vessels, including the umbilical vein. Human umbilical vein endothelial cells (HUVECs) isolated ...

journal_title：Experimental cell research

authors： Hughes SE

更新日期：1996-05-25 00:00:00

abstract：:Metastatic progression is a major cause of mortality in cervical cancers, but factors regulating migratory and pre-metastatic cell populations remain poorly understood. Here, we sought to assess whether a SUV39H1-low chromatin state promotes migratory cell populations in cervical cancers, using meta-analysis of data f...

journal_title：Experimental cell research

authors： Rodrigues C,Pattabiraman C,Vijaykumar A,Arora R,Narayana SM,Kumar RV,Notani D,Varga-Weisz P,Krishna S

更新日期：2019-05-15 00:00:00

abstract：:Several cell division cycle (cdc) mutants of Saccharomyces cerevisiae (cdc28, cdc35, cdc19, cdc21, and cdc17) at the restrictive temperature (37 degrees C) in the presence of 1% glucose and defined medium divert most of the carbon (approximately 50%) to ethanol production with low biomass growth yields (Yglc) that cor...

journal_title：Experimental cell research

authors： Mónaco ME,Valdecantos PA,Aon MA

更新日期：1995-03-01 00:00:00