Abstract:
:We present evidence for the possible involvement of both the RB and p53 proteins in the regulation of cellular senescence. Human fibroblasts immortalized with an inducible SV40 T-antigen become senescent following the de-induction of T-antigen. Plasmids expressing an alternative source of intact T-antigen restore proliferation but T-antigen deletion mutants lacking either the RB or p53 binding domains are unable to do so. Similarly, combinations of adenovirus E1A + E1B or human papillomavirus E6 + E7 genes are able to replace T-antigen functions and permit cell proliferation, whereas the individual genes do not. These results are discussed in terms of a two-stage model for the escape from in vitro cellular senescence.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Shay JW,Pereira-Smith OM,Wright WEdoi
10.1016/0014-4827(91)90453-2keywords:
subject
Has Abstractpub_date
1991-09-01 00:00:00pages
33-9issue
1eissn
0014-4827issn
1090-2422pii
0014-4827(91)90453-2journal_volume
196pub_type
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