A role for both RB and p53 in the regulation of human cellular senescence.

Abstract:

:We present evidence for the possible involvement of both the RB and p53 proteins in the regulation of cellular senescence. Human fibroblasts immortalized with an inducible SV40 T-antigen become senescent following the de-induction of T-antigen. Plasmids expressing an alternative source of intact T-antigen restore proliferation but T-antigen deletion mutants lacking either the RB or p53 binding domains are unable to do so. Similarly, combinations of adenovirus E1A + E1B or human papillomavirus E6 + E7 genes are able to replace T-antigen functions and permit cell proliferation, whereas the individual genes do not. These results are discussed in terms of a two-stage model for the escape from in vitro cellular senescence.

journal_name

Exp Cell Res

authors

Shay JW,Pereira-Smith OM,Wright WE

doi

10.1016/0014-4827(91)90453-2

keywords:

subject

Has Abstract

pub_date

1991-09-01 00:00:00

pages

33-9

issue

1

eissn

0014-4827

issn

1090-2422

pii

0014-4827(91)90453-2

journal_volume

196

pub_type

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