当前位置： SCI文献检索 > EXPERIMENTAL CELL RESEARCH期刊下所有文献 > Subcellular distribution of distinct nucleolin subfractions recognized by two monoclonal antibodies.

Subcellular distribution of distinct nucleolin subfractions recognized by two monoclonal antibodies.

Abstract：

:Monoclonal antibodies binding to different domains of nucleolin have been used to localize nucleolin in tissue culture cells of Xenopus laevis. The monoclonal antibody b6-6E7 binds to an epitope in the N-terminal domain, which contains arrays of phosphorylation consensus sites. This monoclonal antibody binds to nucleolin of oocytes and of eggs with high affinity. In contrast, the monoclonal antibody Nu-1H6 binds poorly to the modified forms of nucleolin arising during meiosis and mitosis. In interphase cells, monoclonal antibody b6-6E7 preferentially stains the periphery of the nucleoli, where most of the rRNA accumulates. Staining by monoclonal antibody Nu-1H6 complements this pattern by staining mainly the center of the nucleoli. The epitope of monoclonal antibody Nu-1H6 is within the central domain of nucleolin, which contains the first two RNA binding domains. RNase treatment of cells results in loss of nucleolin from nucleoli. In mitotic cells, both monoclonal antibodies decorate the surface of condensing chromosomes in prophase. The periphery of the condensed chromosomes in metaphase and anaphase is preferentially stained by monoclonal antibody b6-6E7.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Schwab MS,Gossweiler U,Dreyer C

doi

10.1006/excr.1997.3878

subject

Has Abstract

pub_date

1998-03-15 00:00:00

pages

226-34

issue

2

eissn

0014-4827

issn

1090-2422

pii

S0014482797938787

journal_volume

239

pub_type

杂志文章

相关文献

EXPERIMENTAL CELL RESEARCH文献大全
  • Estrogen receptor alpha (ESR1)-signaling regulates the expression of the taxane-response biomarker PRP4K.

    abstract：:The pre-mRNA splicing factor 4 kinase PRP4K (PRPF4B), is an essential kinase that is a component of the U5 snRNP and functions in spliceosome assembly. We demonstrated that PRP4K is a novel biological marker for taxane response in ovarian cancer patients and reduced levels of PRP4K correlate with intrinsic and acquire...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2015.12.013

    authors： Lahsaee S,Corkery DP,Anthes LE,Holly A,Dellaire G

    更新日期：2016-01-01 00:00:00

  • A syndecan-4 binding peptide derived from laminin 5 uses a novel PKCε pathway to induce cross-linked actin network (CLAN) formation in human trabecular meshwork (HTM) cells.

    abstract：:In this study, we examined the role(s) of syndecan-4 in regulating the formation of an actin geodesic dome structure called a cross-linked actin network (CLAN) in which syndecan-4 has previously been localized. CLANs have been described in several different cell types, but they have been most widely studied in human t...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2014.07.035

    authors： Filla MS,Clark R,Peters DM

    更新日期：2014-10-01 00:00:00

  • Phosphorylation of filamin A by Cdk1 regulates filamin A localization and daughter cell separation.

    abstract：:In cell culture, many adherent mammalian cells undergo substantial actin cytoskeleton rearrangement prior to mitosis as they detach from the extracellular matrix and become spherical. At the end of mitosis, the actin cytoskeleton is required for cytokinesis and the reassembly of interphase structures as cells spread a...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2014.10.024

    authors： Szeto SGY,Williams EC,Rudner AD,Lee JM

    更新日期：2015-01-15 00:00:00

  • Intracellular protein delivery activity of peptides derived from insulin-like growth factor binding proteins 3 and 5.

    abstract：:Insulin-like growth factor binding proteins (IGFBPs) have various IGF-independent cellular activities, including receptor-independent cellular uptake followed by transcriptional regulation, although mechanisms of cellular entry remain unclear. Herein, we focused on their receptor-independent cellular entry mechanism i...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2008.05.008

    authors： Goda N,Tenno T,Inomata K,Shirakawa M,Tanaka T,Hiroaki H

    更新日期：2008-08-01 00:00:00

  • Increment of nonreceptor tyrosine kinase Arg RNA as evaluated by semiquantitative RT-PCR in granulocyte and macrophage-like differentiation of HL-60 cells.

    abstract：:The products of the human Arg gene and human, mouse, Drosophila, and nematode Abl genes characterize the Abelson family of nonreceptor tyrosine protein kinase. The Arg gene, expressed as a 12-kb transcript, codes a protein highly related to c-abl in the tyrosine kinase, SH2, and SH3 domains, and both proteins have a m...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1998.4234

    authors： Perego RA,Bianchi C,Brando B,Urbano M,Del Monte U

    更新日期：1998-11-25 00:00:00

  • Isoforms of p38MAPK gamma and delta contribute to differentiation of human AML cells induced by 1,25-dihydroxyvitamin D₃.

    abstract：:Inhibition of p38MAPK alpha/beta is known to enhance 1,25-dihydroxyvitamin (1,25D)-induced monocytic differentiation, but the detailed mechanism of this effect was not clear. We now show that the enhancement of differentiation becomes apparent with slow kinetics (12-24 h). Interestingly, the inhibition of p38MAPK alph...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2010.08.010

    authors： Zhang J,Harrison JS,Studzinski GP

    更新日期：2011-01-01 00:00:00

  • Aberrant expression of basic fibroblast growth factor in NIH-3T3 cells alters drug resistance and gene amplification potential.

    abstract：:We have investigated the genetic stability of NIH-3T3 cells transfected with sequences coding for basic fibroblast growth factor (bFGF) by determining drug resistance and gene amplification potential. Colony-forming experiments and fluctuation analyses showed that the frequency and rate of resistance to N-(phosphonace...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1994.1207

    authors： Huang A,Jin H,Wright JA

    更新日期：1994-08-01 00:00:00

  • Chitosan oligosaccharides enhance lipid droplets via down-regulation of PCSK9 gene expression in HepG2 cells.

    abstract：:Chitosan oligosaccharides (COS), linear polymers of N-acetyl-D-glucosamine and deacetylated glucosamine, exhibit diverse pharmacological effects such as antimicrobial, antitumor, antioxidant and anti-inflammatory activities. Here, we explored their hypocholesterolemic effects in vivo and the molecular mechanisms of CO...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2018.03.013

    authors： Yang X,Zhang J,Chen L,Wu Q,Yu C

    更新日期：2018-05-15 00:00:00

  • Laminin isoforms: biological roles and effects on the intracellular distribution of nuclear proteins in intestinal epithelial cells.

    abstract：:Laminins are structurally and functionally major components of the extracellular matrix. Four isoforms of laminins (laminin-1, -2, -5 and -10) are expressed in a specific pattern along the crypt-villus axis of the intestine. Previous works indicated that expression of these isoforms is developmentally regulated and th...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2004.10.025

    authors： Turck N,Gross I,Gendry P,Stutzmann J,Freund JN,Kedinger M,Simon-Assmann P,Launay JF

    更新日期：2005-02-15 00:00:00

  • Laminin-induced process outgrowth from isolated fetal rat C-cells.

    abstract：:A method for isolation of C-cells from rat fetuses was developed, and the morphological plasticity of the cells in primary culture systems was tested. Thyroid-parathyroid-ultimobranchial body (UB) complexes from 16-day rat fetuses were treated with 0.1% collagenase and 1000 PU/ml Dispase at 37 degrees C for 1 h. After...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(92)90373-g

    authors： Nishiyama I,Fujii T

    更新日期：1992-02-01 00:00:00

  • Mitochondria control acute and chronic responses to hypoxia.

    abstract：:There are numerous mechanisms by which mammals respond to hypoxia. These include acute changes in pulmonary arterial tone due to smooth muscle cell contraction, acute increases in respiration triggered by the carotid body chemosensory cells, and chronic changes such as induction of red blood cell proliferation and ang...

    journal_title：Experimental cell research

    pub_type： 杂志文章,评审

    doi：10.1016/j.yexcr.2017.03.034

    authors： McElroy GS,Chandel NS

    更新日期：2017-07-15 00:00:00

  • Collagen XIV and a related recombinant fragment protect human vascular smooth muscle cells from calcium-/phosphate-induced osteochondrocytic transdifferentiation.

    abstract：:Transdifferentiation of vascular smooth muscle cells (VSMC) promotes the development of vascular calcifications such as arteriosclerosis. The aim was to investigate effects of specific extracellular matrix (ECM) components on transdifferentiation of VSMC to identify novel ECM-based therapeutic tools. Human collagens I...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2017.06.018

    authors： Freise C,Bobb V,Querfeld U

    更新日期：2017-09-15 00:00:00

  • Factor Xa stimulates fibroblast procollagen production, proliferation, and calcium signaling via PAR1 activation.

    abstract：:Fibroblast proliferation and procollagen production are central features of tissue repair and fibrosis. In addition to its role in blood clotting, the coagulation cascade proteinase thrombin can contribute to tissue repair by stimulating fibroblasts via proteolytic activation of proteinase-activated receptor-1 (PAR1)....

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2004.10.021

    authors： Blanc-Brude OP,Archer F,Leoni P,Derian C,Bolsover S,Laurent GJ,Chambers RC

    更新日期：2005-03-10 00:00:00

  • CIR, a corepressor of CBF1, binds to PAP-1 and effects alternative splicing.

    abstract：:We have reported that PAP-1, a product of a causative gene for autosomal retinitis pigmentosa, plays a role in splicing. In this study, CIR, a protein originally identified as a CBF1-interacting protein and reported to act as a transcriptional corepressor, was identified as a PAP-1 binding protein and its function as ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2004.10.012

    authors： Maita H,Kitaura H,Ariga H,Iguchi-Ariga SM

    更新日期：2005-02-15 00:00:00

  • CD25-expressing Th17 cells mediate CD8+ T cell suppression in CTLA-4 dependent mechanisms in pancreatic ductal adenocarcinoma.

    abstract：:The tumor-associated immune response is governed by the signalling events of various regulatory molecules, one of which is the cytotoxic T lymphocyte-associated antigen 4 (CTLA-4). In conventional T cells, CTLA-4 could outcompete CD28 in binding to CD80/86 but does not produce a co-stimulatory signal, resulting in T c...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2017.09.030

    authors： Lang C,Wang J,Chen L

    更新日期：2017-11-15 00:00:00

  • Induction of tyrosinase in human melanoma cells by L-tyrosine phosphate and cytochalasin D.

    abstract：:Pigmentation of RVH 421 human melanoma cells is induced when cell division is inhibited by cytochalasin D or L-tyrosine phosphate. Increased pigmentation correlates with increased tyrosinase activity when this is monitored over a time-course. Parallel measurements show that the amount of tyrosinase mRNA correlates wit...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(92)90431-7

    authors： Winder AJ,Harris H

    更新日期：1992-04-01 00:00:00

  • In vitro differentiation of bone and hypertrophic cartilage from periosteal-derived cells.

    abstract：:Periosteal cells were enzymatically liberated from the tibiae of young chicks, introduced into cell culture, and allowed to reach confluence. The morphology of the cells gave the impression of a relatively homogeneous population of fibroblast-like cells. These cultured cells did not overtly express osteogenic or chond...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(91)90401-f

    authors： Nakahara H,Dennis JE,Bruder SP,Haynesworth SE,Lennon DP,Caplan AI

    更新日期：1991-08-01 00:00:00

  • Hydrogen peroxide lethality is associated with a decreased ability to maintain positive DNA supercoiling.

    abstract：:Hydrogen peroxide is more toxic to mammalian cells at 37 degrees C than 0 degree C at all concentrations studied. Histone-free nuclei (nucleoids) extracted from treated cells have a reduced ability to maintain positive DNA supercoiling, with the maximum effect at the higher temperature. Prior exposure of cells to sodi...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(92)90088-p

    authors： Vaughan AT,Gordon DJ

    更新日期：1992-10-01 00:00:00

  • Tumor endothelial marker 5 expression in endothelial cells during capillary morphogenesis is induced by the small GTPase Rac and mediates contact inhibition of cell proliferation.

    abstract：:Tumor endothelial marker (TEM) 5 is an adhesion G-protein-coupled receptor upregulated in endothelial cells during tumor and physiologic angiogenesis. So far, the mechanisms leading to upregulation of TEM5 and its function during angiogenesis have not been identified. Here, we report that TEM5 expression in endothelia...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2009.10.013

    authors： Vallon M,Rohde F,Janssen KP,Essler M

    更新日期：2010-02-01 00:00:00

  • Elimination of mycoplasmas from cell cultures utilizing hyperimmune sera.

    abstract：:Eighteen cell lines contaminated with various mycoplasmas have been treated with hyperimmune sera and mycoplasmas have been eradicated from all. After treatment the cell lines have been observed for a least one year and they are still free from mycoplasma contamination as ascertained by four independent mycoplasma det...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(85)90502-6

    authors： Jeansson S,Brorson JE

    更新日期：1985-11-01 00:00:00

  • Identification of the type-B receptor for platelet-derived growth factor in human embryonal carcinoma cells.

    abstract：:The human embryonal carcinoma (EC) cell line Tera 2 clone 13 (T2/13) can be induced to differentiate in vitro into neuroectodermal cell types with retinoic acid. Undifferentiated cells are characterized by rapid proliferation, whereas differentiated cells show a prolonged generation time, have a limited life span, and...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(90)90312-x

    authors： Weima SM,van Rooijen MA,Mummery CL,Feyen A,de Laat SW,van Zoelen EJ

    更新日期：1990-02-01 00:00:00

  • Species-divergent regulation of human and mouse osteocalcin genes by calciotropic hormones.

    abstract：:Although osteocalcin is the most abundant noncollagenous protein in bone, its role remains undefined. Recent studies have reported diametrically opposing responses in the vitamin D regulation of the mouse vs the human and rat osteocalcin genes. The aim of this study was to increase the understanding of these differenc...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2000.4912

    authors： Thomas GP,Bourne A,Eisman JA,Gardiner EM

    更新日期：2000-08-01 00:00:00

  • Localization of a gene involved in complementation of the defect in xeroderma pigmentosum group A cells on human chromosome 1.

    abstract：:Human, Chinese hamster or Chinese hamster/human hybrid cytoplasts were fused with UV-irradiated xeroderma pigmentosum group A (XP-A) cells. Unscheduled DNA synthesis (UDS) of the XP-A nucleus was measured 0-2 and 2-4 h after seeding of the fused population. Human cytoplasts did correct the defect in the XP-A nucleus i...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(87)90209-6

    authors： Keijzer W,Stefanini M,Bootsma D,Verkerk A,Geurts van Kessel AH,Jongkind JF,Westerveld A

    更新日期：1987-04-01 00:00:00

  • The trafficking and regulation of membrane receptors by the RING-CH ubiquitin E3 ligases.

    abstract：:Ubiquitylation of membrane receptors is recognised as a critical post-translational modification, governing their regulation and function. Following ubiquitylation, membrane proteins may be internalised, recycled or degraded via lysosomal or proteasomal pathways. Viruses have appropriated these cellular pathways as a ...

    journal_title：Experimental cell research

    pub_type： 杂志文章,评审

    doi：10.1016/j.yexcr.2008.10.026

    authors： Nathan JA,Lehner PJ

    更新日期：2009-05-15 00:00:00

  • Exchange of chemical signals between cardiac cells. Fundamental role on cell communication and metabolic cooperation.

    abstract：:The exchange of chemical signals between cardiac cells and its relevance for cell communication and metabolic cooperation was reviewed. The role of gap junctions on the transfer of chemical information was discussed as well as the different factors involved in its regulation including changes in cell volume, high gluc...

    journal_title：Experimental cell research

    pub_type： 杂志文章,评审

    doi：10.1016/j.yexcr.2016.05.009

    authors： De Mello WC

    更新日期：2016-08-01 00:00:00

  • Cytotoxic T cell responses to Streptococcus are associated with improved prognosis of oral squamous cell carcinoma.

    abstract：:Several species of Streptococcus, such as S. salivarius, S. mitis, and S. anginosus, are found to extensively colonize the oral cavity and the upper respiratory tract, and have been shown to increase in patients with oral squamous cell carcinoma (OSCC). Accumulating evidence have revealed that commensal bacteria are i...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2017.11.018

    authors： Wang J,Sun F,Lin X,Li Z,Mao X,Jiang C

    更新日期：2018-01-01 00:00:00

  • Demonstration of a 7-nm RNP fiber as the basic structural element in a premessenger RNP particle.

    abstract：:Balbiani ring granules in Chironomus salivary glands represent premessenger ribonucleoprotein (RNP) particles, each containing a 35- to 40-kb message for a secretory polypeptide. Their gross structure can be described as an RNP ribbon bent into a toroid. We now demonstrate that an unfolded, thin RNP fiber is observed ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(92)90437-d

    authors： Lönnroth A,Alexciev K,Mehlin H,Wurtz T,Skoglund U,Daneholt B

    更新日期：1992-04-01 00:00:00

  • Polo-like kinase 3 is Golgi localized and involved in regulating Golgi fragmentation during the cell cycle.

    abstract：:The Golgi apparatus undergoes extensive fragmentation during mitosis in animal cells. Protein kinases play a critical role during fragmentation of the Golgi apparatus. We reported here that Polo-like kinase 3 (Plk3) may be an important mediator during Golgi breakdown. Specifically, Plk3 was concentrated at the Golgi a...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2003.10.022

    authors： Ruan Q,Wang Q,Xie S,Fang Y,Darzynkiewicz Z,Guan K,Jhanwar-Uniyal M,Dai W

    更新日期：2004-03-10 00:00:00

  • Acute myocardial infarction leads to upregulation of the IGF-1 autocrine system, DNA replication, and nuclear mitotic division in the remaining viable cardiac myocytes.

    abstract：:Insulin-like growth factor-1 (IGF-1) and its receptor (IGF-1R) are required for cell proliferation in vitro, raising the possibility that an autocrine IGF-1-IGF-1R system may be present in vivo and become activated in the viable ventricular myocytes shortly after infarction. Therefore, following the in vivo documentat...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1994.1224

    authors： Reiss K,Kajstura J,Zhang X,Li P,Szoke E,Olivetti G,Anversa P

    更新日期：1994-08-01 00:00:00

  • Transforming growth factor-beta 1 has both promoting and inhibiting effects on induction of DNA synthesis in human fibroblasts.

    abstract：:Growth-arrested human normal fibroblasts, TIG-1, initiated DNA synthesis following addition of epidermal growth factor (EGF). Transforming growth factor-beta 1 (TGF-beta 1) by itself had no effect on induction of DNA synthesis. When EGF and TGF-beta 1 were added simultaneously to growth-arrested TIG-1 cells, induction...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1995.1014

    authors： Fukami J,Tsuji K,Ueno A,Ide T

    更新日期：1995-01-01 00:00:00