Characterization of lamina-bound chromatin in the nuclear shell isolated from HeLa cells.

Abstract:

:The structure and the polypeptide composition of the nuclear shell isolated from interphase HeLa cells have been investigated and compared to those of the intranuclear material. The isolated nuclear shell contains chromatin superstructures (28-32 nm thick fibres) made of tightly packed nucleosomes that resist low ionic strength conditions and that are associated with the three nuclear lamins. Chromatin in the nuclear shell exhibits very simple chemical composition. Especially, non-histone proteins are lacking. The results presented here rule out the possibility that the nuclear shell results from contamination of lamina by intranuclear elements. They suggest that the lamins are directly involved in the specific properties and in the organization of chromatin in the nuclear shell.

journal_name

Exp Cell Res

authors

Bouvier D,Hubert J,Seve AP,Bouteille M

doi

10.1016/0014-4827(85)90557-9

subject

Has Abstract

pub_date

1985-02-01 00:00:00

pages

500-12

issue

2

eissn

0014-4827

issn

1090-2422

journal_volume

156

pub_type

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