Abstract：
:In many systems, events participating in cell division are controlled by intracellular pH (pHi). In Xenopus eggs, fertilization is accompanied by an increase in pHi which occurs concomitantly with an increase in protein synthesis and a reinitiation of DNA synthesis, leading the embryo to cell division. In this paper, we have shown that increasing pHi of fertilized eggs from 7.8 to 8.2 by using weak bases produced an arrest in embryonic development. Such a change in pHi was accompanied by a severe inhibition of both protein and DNA syntheses. In order to discriminate between a direct effect of pHi and a pH-independent effect of weak bases on these biosyntheses, the situation was studied in vitro. For this purpose, cytoplasmic extracts were used in which weak base addition did not produce any change in pH. Under these conditions, protein synthesis was not inhibited, suggesting that pH is probably one of the events implicated in the regulation of protein synthesis. On the other hand, DNA synthesis was inhibited by weak bases in vitro, without any change in pH intervening.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Grandin N,Charbonneau Mdoi
10.1016/0014-4827(89)90313-3subject
Has Abstractpub_date
1989-12-01 00:00:00pages
436-52issue
2eissn
0014-4827issn
1090-2422journal_volume
185pub_type
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