Abstract：
:The giant protein hFat1, a member of the cadherin superfamily, has been proposed to play roles in cerebral development, glomerular slit formation, and also to act as a tumor suppressor, but its mechanisms of action have not been elucidated. To examine functions of the transmembrane and cytoplasmic domains, they were expressed in HEK293 and HeLa cells as chimeric proteins in fusion with EGFP and extracellular domains derived from E-cadherin. Proteins comprising the transmembrane domain localized to the membrane fraction. Deletion of this domain resulted in a predominantly nuclear localization of the cytoplasmic segment of hFat1. Nuclear localization was largely reduced by deletion of a presumed juxta-membrane NLS. Fusion proteins located in the plasma membrane underwent proteolytic processing. In a first proteolytic step, only the extracellular domain was cleaved off. In another step, the cleavage product was released to the cytosol and was also found in a low speed pellet fraction, in accordance with the nuclear localization of the cytoplasmic domain of hFat1.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Magg T,Schreiner D,Solis GP,Bade EG,Hofer HWdoi
10.1016/j.yexcr.2005.03.006keywords:
subject
Has Abstractpub_date
2005-07-01 00:00:00pages
100-8issue
1eissn
0014-4827issn
1090-2422pii
S0014-4827(05)00113-8journal_volume
307pub_type
杂志文章abstract：:Eighteen cell lines contaminated with various mycoplasmas have been treated with hyperimmune sera and mycoplasmas have been eradicated from all. After treatment the cell lines have been observed for a least one year and they are still free from mycoplasma contamination as ascertained by four independent mycoplasma det...
journal_title：Experimental cell research
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abstract：:Melanoma formation in the teleost fish Xiphophorus is caused by the uncontrolled activity of the genetically defined tumor locus Tu. The critical component of this locus is the Xmrk oncogene encoding a subclass I receptor tyrosine kinase. Overexpression and constitutive activation of the Xmrk receptor triggers a set o...
journal_title：Experimental cell research
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journal_title：Experimental cell research
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doi：10.1006/excr.1999.4432
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journal_title：Experimental cell research
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journal_title：Experimental cell research
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journal_title：Experimental cell research
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doi：10.1016/0014-4827(92)90072-g
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journal_title：Experimental cell research
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doi：10.1016/j.yexcr.2007.04.010
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1995.1148
更新日期：1995-05-01 00:00:00
abstract：:The loss of expression of a single gene can revert normal tissue to a malignant phenotype. For example, while normal breast has high lumenal expression of CEACAM1, the majority of breast cancers exhibit the early loss of this gene with the concurrent loss of their lumenal phenotype. MCF7 cells that lack CEACAM1 expres...
journal_title：Experimental cell research
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doi：10.1016/j.yexcr.2017.08.008
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abstract：:The epithelial cell adhesion molecule Ep-CAM is capable of mediating Ca2+-independent homotypic cell-cell adhesion when introduced into cells lacking their own means of cell-cell interactions. We used (confocal) immunofluorescent and (immuno-) electron microscopy to investigate the structural organization of Ep-CAM-me...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1998.4263
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journal_title：Experimental cell research
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journal_title：Experimental cell research
pub_type： 杂志文章
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journal_title：Experimental cell research
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journal_title：Experimental cell research
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journal_title：Experimental cell research
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abstract：:To determine which endocytic compartments are sensitive to sucrose-induced osmotic swelling, CHO and Vero cells were cultured for 1-3 days in media containing 0.03 to 0.05 M sucrose. (Sucrose is internalized but not digested by these cells.) To immunolocalize late endocytic compartments, cells were fixed with formalde...
journal_title：Experimental cell research
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abstract：:SMN, the affected protein in spinal muscular atrophy (SMA), is a cytoplasmic protein that also occurs in nuclear structures called "gems" and is involved in snRNP maturation. Coilin-p80 is a marker protein for nuclear Cajal bodies (coiled bodies; CBs) which are also involved in snRNP maturation, storage or transport. ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.2001.5186
更新日期：2001-05-01 00:00:00
