Microinjected catalytic subunit of cAMP-dependent protein kinase induces apoptosis in myeloid leukemia (IPC-81) cells.

abstract：:Strategies for correct diagnosis, treatment evaluation and recurrence prediction are important for the prognosis and mortality rates among cancer patients. In spite of major improvements in clinical management, gastrointestinal stromal tumors (GISTs) can still be deadly due to metastasis and recurrences, which confirm...

journal_title：Experimental cell research

authors： Berglund E,Daré E,Branca RM,Akcakaya P,Fröbom R,Berggren PO,Lui WO,Larsson C,Zedenius J,Orre L,Lehtiö J,Kim J,Bränström R

更新日期：2015-08-01 00:00:00

abstract：:We established a differentiation method for homogeneous α7 integrin-positive human skeletal muscle stem cell (α7(+)hSMSC)-derived osteoblast-like cells with bone morphogenetic protein (BMP)-2. To explore the early signaling cascade for osteoblastic differentiation, we examined the upregulation of autophagy-related gen...

journal_title：Experimental cell research

authors： Ozeki N,Mogi M,Hase N,Hiyama T,Yamaguchi H,Kawai R,Matsumoto T,Nakata K

更新日期：2016-09-10 00:00:00

abstract：:The recruitment of osteoblast progenitors involves their migration and attachment to the sites of bone formation through interactions with matrix proteins. In a time-limited cell attachment assay, coated laminin-1 inhibits the adhesion of most rat calvaria cells but attaches specifically to osteoprogenitors, as quanti...

journal_title：Experimental cell research

authors： Roche P,Rousselle P,Lissitzky JC,Delmas PD,Malaval L

更新日期：1999-08-01 00:00:00

abstract：:Inverted thyroid follicles in suspension culture were observed using dark-field illumination and time-lapse video enhancement techniques. The lumens of many follicles undergo episodes of rapid shrinkage followed by relatively slow dilation. The extent of shrinkage ranged from 10 to 40% of the initial diameter. The dec...

journal_title：Experimental cell research

authors： Nitsch L,Wollman SH

更新日期：1986-01-01 00:00:00

abstract：:A temperature-sensitive mutant of rat fibroblast line 3Y1, 3Y1tsD123, is arrested in the G1 phase of the cell cycle at restrictive temperature. Using a human cDNA expression library, we succeeded in the molecular cloning of a cDNA (designated as D123 cDNA) that functionally complemented the temperature-sensitive mutat...

journal_title：Experimental cell research

authors： Okuda A,Kimura G

更新日期：1996-03-15 00:00:00

abstract：:The abundant heterogeneous nuclear ribonucleoprotein M (hnRNP M) is able to associate with early spliceosomes and to influence splicing patterns of specific pre-mRNAs. Here, by a combination of immunoprecipitation and pull-down assays, we have identified PSF (polypyrimidine tract-binding protein-associated splicing fa...

journal_title：Experimental cell research

authors： Marko M,Leichter M,Patrinou-Georgoula M,Guialis A

更新日期：2010-02-01 00:00:00

abstract：:The KIAA0101/p15(PAF)/OEATC-1 protein was initially isolated in a yeast two-hybrid screen for proliferating cell nuclear antigen (PCNA) binding partners, and was shown to bind PCNA competitively with the cell cycle regulator p21(WAF). PCNA is involved in DNA replication and damage repair. Using polyclonal antisera rai...

journal_title：Experimental cell research

authors： Simpson F,Lammerts van Bueren K,Butterfield N,Bennetts JS,Bowles J,Adolphe C,Simms LA,Young J,Walsh MD,Leggett B,Fowles LF,Wicking C

更新日期：2006-01-01 00:00:00

abstract：:Previous studies have shown that calpains are autolytically cleaved during the disease process of mdx dystrophy, a mouse model for Duchenne muscular dystrophy, indicating that calpains may be activated and play a role in proteolysis that occurs in muscular dystrophy (J. Biol. Chem. 270(18), 10909-10914, 1995). In the ...

journal_title：Experimental cell research

authors： Spencer MJ,Tidball JG

更新日期：1996-08-01 00:00:00

abstract：:Myosin phosphatase target subunit 1 (MYPT1), together with catalytic subunit of type1 delta isoform (PP1cdelta) and a small 20-kDa regulatory unit (M20), form a heterotrimeric holoenzyme, myosin phosphatase (MP), which is responsible for regulating the extent of myosin light chain phosphorylation. Here we report the i...

journal_title：Experimental cell research

authors： Twomey E,Li Y,Lei J,Sodja C,Ribecco-Lutkiewicz M,Smith B,Fang H,Bani-Yaghoub M,McKinnell I,Sikorska M

更新日期：2010-01-01 00:00:00

abstract：:The migration and proliferation of vascular smooth muscle cells (vSMCs) are critical events in neointima formation during atherosclerosis and restenosis. The transcription factor nuclear factor of activated T-cells-isoform c1 (NFATc1) is regulated by atherogenic cytokines, and has been implicated in the migratory and ...

journal_title：Experimental cell research

authors： Chow W,Hou G,Bendeck MP

更新日期：2008-10-01 00:00:00

abstract：:Formation of the neural tube is the morphological hallmark for development of the embryonic central nervous system (CNS). Therefore, neural tube development is a crucial step in the neurulation process. Slit/Robo signaling was initially identified as a chemo-repellent that regulated axon growth cone elongation, but it...

journal_title：Experimental cell research

authors： Wang G,Li Y,Wang XY,Han Z,Chuai M,Wang LJ,Ho Lee KK,Geng JG,Yang X

更新日期：2013-05-01 00:00:00

abstract：:Idiopathic pulmonary fibrosis (IPF), a progressive disorder of unknown etiology, is characterized by pathological lung fibroblast activation and proliferation resulting in abnormal deposition of extracellular matrix proteins within the lung parenchyma. The pathophysiological roles of exosomal microRNAs in pulmonary fi...

journal_title：Experimental cell research

authors： Inomata M,Kamio K,Azuma A,Matsuda K,Usuki J,Morinaga A,Tanaka T,Kashiwada T,Atsumi K,Hayashi H,Fujita K,Saito Y,Kubota K,Seike M,Gemma A

更新日期：2021-01-15 00:00:00

abstract：:Hydrogen peroxide is more toxic to mammalian cells at 37 degrees C than 0 degree C at all concentrations studied. Histone-free nuclei (nucleoids) extracted from treated cells have a reduced ability to maintain positive DNA supercoiling, with the maximum effect at the higher temperature. Prior exposure of cells to sodi...

journal_title：Experimental cell research

authors： Vaughan AT,Gordon DJ

更新日期：1992-10-01 00:00:00

abstract：:Progressive pathophysiologic modifications of endothelial cells are associated with aging. In vitro, endothelial cell senescence is accompanied by the failure to proliferate as well as by perturbations in gene expression. Here we show that (i) senescence enhances monoblastoid U937 cell adhesion to the endothelial mono...

journal_title：Experimental cell research

authors： Maier JA,Statuto M,Ragnotti G

更新日期：1993-09-01 00:00:00

abstract：:Protein C23 (also called nucleolin or 100-kDa nucleolar protein) is a major nucleolar phosphoprotein involved in ribosome biogenesis. To determine the effects of protein C23 on preribosomal RNA (pre-rRNA) synthesis anti-C23 antiserum was microinjected into nuclei of Chironomus tentans salivary glands. Transcription wa...

journal_title：Experimental cell research

authors： Egyhazi E,Pigon A,Chang JH,Ghaffari SH,Dreesen TD,Wellman SE,Case ST,Olson MO

更新日期：1988-10-01 00:00:00

abstract：OBJECTIVES:Our purpose was to investigate the role of the nitric oxide (NO) signaling pathway in the regulation of ciliary beat frequency (CBF) in mouse nasal and tracheal epithelial cells. METHODS:We studied the effects of the NO donor l-arginine (L-Arg) and specific inhibitors of the NO signaling pathway on CBF of b...

journal_title：Experimental cell research

authors： Jiao J,Wang H,Lou W,Jin S,Fan E,Li Y,Han D,Zhang L

更新日期：2011-10-15 00:00:00

abstract：:Embryonic stem (ES) cell lines have been derived from blastocysts of the inbred mouse strain C57BL/6. The highest frequencies of ES cell colonies were observed when blastocysts were explanted directly onto growth-arrested feeder layers of 5637 human bladder carcinoma cells in the presence of conditioned medium. One of...

journal_title：Experimental cell research

authors： Ledermann B,Bürki K

更新日期：1991-12-01 00:00:00

abstract：:Herp (homocysteine-induced endoplasmic reticulum protein) is an ER-resident membrane protein, which has a ubiquitin-like domain at its N-terminus. Expression of Herp protein is up-regulated in response to ER stress, including homocysteine. Herp stabilizes neuronal Ca(2+) homeostasis and is involved in improving the ba...

journal_title：Experimental cell research

authors： Lenz B,Bleich S,Beutler S,Schlierf B,Schwager K,Reulbach U,Kornhuber J,Bönsch D

更新日期：2006-12-10 00:00:00

abstract：:Multipotent mesenchymal stromal cells (MSCs) support wound healing processes. These cells express toll-like receptors (TLRs). TLRs perform important key functions when the immune system is confronted with danger signals. TLR ligation by lipopolysaccharides (LPS) activates MSCs and induces intracellular signaling casca...

journal_title：Experimental cell research

authors： Ruhl T,Kim BS,Beier JP

更新日期：2018-09-15 00:00:00

abstract：:Src-family kinases, cytoplasmic enzymes that participate in various signaling events, are found at not only the plasma membrane but also subcellular compartments, such as the nucleus, the Golgi apparatus and late endosomes/lysosomes. Lyn, a member of the Src-family kinases, is known to play a role in DNA damage respon...

journal_title：Experimental cell research

authors： Ikeda K,Nakayama Y,Togashi Y,Obata Y,Kuga T,Kasahara K,Fukumoto Y,Yamaguchi N

更新日期：2008-11-01 00:00:00

abstract：:ZO-2 is a MAGUK protein that in confluent epithelial sheets localizes at tight junctions (TJ) whereas in sparse cultures accumulates in clusters at the nucleus. Here, we have characterized several nuclear properties of ZO-2. We observe that ZO-2 is present in the nuclear matrix and co-immunoprecipitates with lamin B(1...

journal_title：Experimental cell research

authors： Jaramillo BE,Ponce A,Moreno J,Betanzos A,Huerta M,Lopez-Bayghen E,Gonzalez-Mariscal L

更新日期：2004-07-01 00:00:00

abstract：:The influence of the cylindrical substratum surface on the actin microfilament bundle and the extracellular matrix (fibronectin and laminin) patterns in nontransformed epithelial cells of the IAR-2 rat line and in their N-ras-transformed descendants, IAR-ras-c4 cells, was studied. The cells were cultured on substrata ...

journal_title：Experimental cell research

authors： Levina EM,Domnina LV,Rovensky YA,Vasiliev JM

更新日期：1996-11-25 00:00:00

abstract：:Annexin 2 is a Ca(2+)-dependent phospholipid-binding protein that is involved in secretion. Despite the fact that this protein does not have signals for its secretion, many reports have shown its presence in the extracellular milieu. Here we demonstrate that, upon stimulation of exocytosis in chromaffin cells, a fract...

journal_title：Experimental cell research

authors： Faure AV,Migné C,Devilliers G,Ayala-Sanmartin J

更新日期：2002-05-15 00:00:00

abstract：RATIONALE:Vessel formation is a crucial event in tissue repair after injury. Thus, one assumption of innovative therapeutic approaches is the understanding of its molecular mechanisms. Notwithstanding our knowledge of the role of Protein Kinase C epsilon (PKCε) in cardio-protection and vascular restenosis, its role in ...

journal_title：Experimental cell research

authors： Galli D,Carubbi C,Masselli E,Corradi D,Dei Cas A,Nouvenne A,Bucci G,Arcari ML,Mirandola P,Vitale M,Gobbi G

更新日期：2015-01-15 00:00:00

abstract：:Phosphorylation of the nucleosome core histone H3 (H3) on Ser-10 is thought to be a prerequisite for chromatin condensation at mitosis. Although during interphase, cell differentiation, or mitogenic activation of quiescent cells, changes in chromatin structure that involve local chromatin condensation/decondensation a...

journal_title：Experimental cell research

authors： Juan G,Traganos F,Darzynkiewicz Z

更新日期：1999-01-10 00:00:00

abstract：:The HRP-1 cell line is derived from normal rat placenta and appears morphologically similar to and retains characteristic expression of cellular markers of labyrinthine trophoblast cells. In this study, monolayers of HRP-1 cells grown on permeable supports were evaluated as a potential in vitro system to study trophob...

journal_title：Experimental cell research

authors： Shi F,Soares MJ,Avery M,Liu F,Zhang X,Audus KL

更新日期：1997-07-10 00:00:00

abstract：:Based on recent studies showing that PLCgamma associates to insulin receptor, we investigated its role in insulin stimulation of glucose transport in brown adipocytes. Insulin stimulation induced rapid PLCgamma association to phosphorylated insulin receptor, and activation of PLCgamma, as assessed by the mobilization ...

journal_title：Experimental cell research

authors： Lorenzo M,Teruel T,Hernandez R,Kayali AG,Webster NJ

更新日期：2002-08-15 00:00:00

abstract：:Interleukin 3 (IL-3) stimulates the growth of various types of hemopoietic progenitors. In vitro, survival of a series of murine cell lines derived from either neoplastic or nonneoplastic hemopoietic tissue shows a strict IL-3 dependence. In order to test the implication of energy metabolism in this dependence as clai...

journal_title：Experimental cell research

authors： Reisbach G,Ellwart J,Dörmer P

更新日期：1990-10-01 00:00:00

abstract：:Mitochondria are increasingly recognized as key mediators of acute cellular stress responses in asthma. However, the distinct roles of regulators of mitochondrial physiology on allergic asthma phenotypes are currently unknown. The mitochondrial Ca2+ uniporter (MCU) resides in the inner mitochondrial membrane and contr...

journal_title：Experimental cell research

authors： Sebag SC,Koval OM,Paschke JD,Winters CJ,Comellas AP,Grumbach IM

更新日期：2018-01-15 00:00:00

abstract：:Liver endothelium has been reported to possess membrane receptors for the iron-binding protein transferrin (Tf). Similarly, the core protein of proteoglycans (PG) associated with cell membrane in many cell systems can bind Tf. To find out if membrane-associated proteoglycans can explain Tf-binding ability of liver end...

journal_title：Experimental cell research

authors： Omoto E,Minguell JJ,Tavassoli M

更新日期：1990-03-01 00:00:00