Lactate production and amino acid incorporation in interleukin 3-dependent, factor-deprived hemopoietic murine cell lines.

abstract：:A novel methodology for labeling, isolation, and detection of nucleic acids is described. Nucleic acid isolation is based on in vivo or in vitro incorporation of BrU or BrdU to either RNA or DNA, respectively, followed by immunoprecipitation of the labeled nucleic acid utilizing anti-BrdU MoAb, which crossreacts with ...

journal_title：Experimental cell research

authors： Haider SR,Juan G,Traganos F,Darzynkiewicz Z

更新日期：1997-08-01 00:00:00

abstract：:Upregulated ERK1/2 activity is often correlated with AKT activation during prostate cancer (PCa) progression, yet their functional relation needs elucidation. Using androgen-deprived LNCaP cells, in which ERK1/2 activation occurs in strong correlation with AKT activation, we found that AKT-mediated B-Raf regulation is...

journal_title：Experimental cell research

authors： Hong SK,Jeong JH,Chan AM,Park JI

更新日期：2013-07-15 00:00:00

abstract：:The ultrastructural localization of adenylate cyclase was studied in bovine cumulus-oocyte complexes. Adenylate cyclase was observed on the plasma membrane of the oocyte and occasionally on the plasma membrane of cumulus cells. The cytochemical observations presented demonstrate that there is more adenylate cyclase in...

journal_title：Experimental cell research

authors： Kuyt JR,Kruip TA,de Jong-Brink M

更新日期：1988-01-01 00:00:00

abstract：:We describe a sequential staining technique for the karyotypic analysis of interspecific mouse x human somatic cell hybrids. Fluorescence in situ hybridization of samples, previously stained using standard trypsin/Giemsa protocols, was instrumental in the identification of human chromosomes present in hybrid lines. Th...

journal_title：Experimental cell research

authors： Keherly MJ,Papaconstantinou J,McCombs JL

更新日期：1993-04-01 00:00:00

abstract：:Transcription factors Mitf and NFATc1 share many downstream targets that are critical for osteoclastogenesis. Since RANKL signals induce/activate both NFATc1 and Mitf isoform-E (Mitf-E), a tissue-restricted Mitf isoform in osteoclasts, it is plausible that the two factors work together to promote osteoclastogenesis. A...

journal_title：Experimental cell research

authors： Lu SY,Li M,Lin YL

更新日期：2014-10-15 00:00:00

abstract：:Fibroblast migration is a central process in skin wound healing, which requires the coordination of several types of growth factors. bFGF, a well-known fibroblast growth factor (FGF), is able to accelerate fibroblast migration; however, the underlying mechanism of bFGF regulation fibroblast migration remains unclear. ...

journal_title：Experimental cell research

authors： Zhu ZX,Sun CC,Ting Zhu Y,Wang Y,Wang T,Chi LS,Cai WH,Zheng JY,Zhou X,Cong WT,Li XK,Jin LT

更新日期：2017-06-15 00:00:00

abstract：:FTO, a demethylase for N6-methyladenosine (m6A) modification, has been implicated in multiple tumors. However, its roles in esophageal squamous cell carcinoma (ESCC) remain uncovered. This study aims to evaluate the clinical relevance and functional roles in this disease. Through immunohistochemistry, qRT-PCR and West...

journal_title：Experimental cell research

authors： Liu S,Huang M,Chen Z,Chen J,Chao Q,Yin X,Quan M

更新日期：2020-04-01 00:00:00

abstract：:The expression of human cellular fibronectin (HFN) was analyzed in relation to the differentiation process of the human enterocyte-like cell line Caco-2. A single approximately 290-kDa form of HFN, corresponding to a 10.0-kb mRNA species, was observed in Caco-2 cells. The expression levels of both protein and mRNA wer...

journal_title：Experimental cell research

authors： Vachon PH,Simoneau A,Herring-Gillam FE,Beaulieu JF

更新日期：1995-01-01 00:00:00

abstract：:It has been generally accepted that alternatively activated macrophages (M2), which can be induced by type 2 cytokines such as IL-4, is responsible for tissue repair. However, the function of JNK in IL-4-induced M2 macrophage polarization remains unclear. Here, we demonstrated that M0 macrophages can be polarized into...

journal_title：Experimental cell research

authors： Hao J,Hu Y,Li Y,Zhou Q,Lv X

更新日期：2017-08-15 00:00:00

abstract：:Microglia, the parenchymal immune cells of the central nervous system, orchestrate neuroinflammation in response to infection or damage, and promote tissue repair. However, aberrant microglial responses are integral to neurodegenerative diseases and critically contribute to disease progression. Thus, it is important t...

journal_title：Experimental cell research

authors： Fodelianaki G,Lansing F,Bhattarai P,Troullinaki M,Zeballos MA,Charalampopoulos I,Gravanis A,Mirtschink P,Chavakis T,Alexaki VI

更新日期：2019-04-15 00:00:00

abstract：:We have isolated a monoclonal antibody, P4B2, which localizes to multiple anchorage junctions, namely, a subset of focal adhesions, the Z-disk of muscle, and neuromuscular junctions. Immunopurification of the antigen to this antibody from chicken brain tissue yielded a complex of three prominent proteins with mobiliti...

journal_title：Experimental cell research

authors： McDonald KA,Muschler J,Horwitz AF

更新日期：1998-09-15 00:00:00

abstract：:Relay of information from the extracellular environment into the cell often results from a peptide growth factor binding to its cognate cell surface receptor; this event is an integral mechanism by which many cellular functions occur, including cell growth, motility, and survival. In recent years, however, this requir...

journal_title：Experimental cell research

authors： Boerner JL,Danielsen A,Maihle NJ

更新日期：2003-03-10 00:00:00

abstract：:In a previous paper (Barboro et al., 1993, Biophys. J. 65, 1690-1699) we have shown that cancer development in the resistant hepatocyte model of Solt and Farber is characterized by the progressive unfolding of the higher-order structure of chromatin. A possible functional role of decondensation phenomena in cell trans...

journal_title：Experimental cell research

authors： Barboro P,Alberti I,Sanna P,Parodi S,Balbi C,Allera C,Patrone E

更新日期：1996-06-15 00:00:00

abstract：:The phosphorylation of glucose-regulated protein, GRP78, is thought to be involved in the regulation of the binding function of GRP78 to immunoglobulin heavy chains. The phosphorylation of GRP78 proceeded faster in transformed cells than in normal cells, whereas the levels of GRP78 synthesis and accumulation were simi...

journal_title：Experimental cell research

authors： Satoh M,Nakai A,Sokawa Y,Hirayoshi K,Nagata K

更新日期：1993-03-01 00:00:00

abstract：:The specific action of a pig skin fraction enriched in epidermal G1-chalone, a tissue-specific inhibitor of epidermal DNA synthesis, was investigated by means of flow cytofluorometry. The results indicate that G1-chalone inhibits progression of partially synchronized rat tongue epithelial cells (line RTE-2) through th...

journal_title：Experimental cell research

authors： Tiegel A,Richter KH,Marks F

更新日期：1989-06-01 00:00:00

abstract：:PDGF acts as an autocrine and paracrine factor in certain tumors through upregulation of the PDGF beta-receptor expression. In order to elucidate the control mechanism for the receptor expression, we have isolated an enhancer from two P1 clones that together contain a 102 kb NotI region covering the entire human PDGFR...

journal_title：Experimental cell research

authors： Kaneko M,Yang W,Matsumoto Y,Watt F,Funa K

更新日期：2006-07-01 00:00:00

abstract：:ADP-ribosylation factor 3 (ARF3) is a member of the KRAS proto-oncogene, GTPase(Ras) super-family of guanine nucleotide-binding proteins that mediates Golgi-related mitosis, but its role in malignant cells is unclear. In the present study, we found that mRNA and protein expression of ARF3 is up-regulated in breast can...

journal_title：Experimental cell research

authors： Huang D,Pei Y,Dai C,Huang Y,Chen H,Chen X,Zhang X,Lin C,Wang H,Zhang R,Wan X,Wang L

更新日期：2019-11-15 00:00:00

abstract：:Fibroblasts derived from rabbit anterior cruciate and medial collateral ligaments were found to constitutively produce and secrete a plasminogen activator (PA). The PA was identified as urokinase-like using biochemical, immunological, and molecular techniques. These fibroblasts also produced and secreted low levels of...

journal_title：Experimental cell research

authors： Murphy PG,Frank CB,Hart DA

更新日期：1993-03-01 00:00:00

abstract：:To study cell motility in different phases of the cell cycle, time-lapse recording by computer-assisted microscopy of unsynchronised cells from three mammalian cell lines (L929, BT4Cn, HeLa) was used for the determination of the displacements of individual cells. The displacements were used for calculation of three ke...

journal_title：Experimental cell research

authors： Walmod PS,Hartmann-Petersen R,Prag S,Lepekhin EL,Röpke C,Berezin V,Bock E

更新日期：2004-05-01 00:00:00

abstract：:Clear cell renal cell carcinoma (ccRCC) is one of the most common malignant tumors in the urinary system, and its incidence continues to increase. Regulator of calcineurin 1 (RCAN1), one of the genes on chromosome 21, is a crucial mediator of tumor inhibition. RCAN1.4 is best characterized as an endogenous inhibitor o...

journal_title：Experimental cell research

authors： Song Z,Cao Q,Ruan H,Yang H,Wang K,Bao L,Cheng G,Xu T,Xiao H,Wang C,Liu D,Chen K,Zhang X

更新日期：2018-11-15 00:00:00

abstract：UNLABELLED:The success of hepatocyte transplantation has been limited by the low efficiency of transplanted cell integration into liver parenchyma. Human fetal hepatic progenitor cells (hepatoblasts) engraft more effectively than adult hepatocytes in mouse livers. However, the signals required for their integration are...

journal_title：Experimental cell research

authors： Delgado JP,Vanneaux V,Branger J,Touboul T,Sentilhes L,Mainot S,Lainas P,Leclerc P,Uzan G,Mahieu-Caputo D,Weber A

更新日期：2009-11-15 00:00:00

abstract：:The glycolytic enzyme aldolase is concentrated in a domain around stress fibers in living Swiss 3T3 cells, but the mechanism by which aldolase is localized has not been revealed. We have recently identified a molecular binding site for F-actin on aldolase, and we hypothesized that this specific binding interaction, ra...

journal_title：Experimental cell research

authors： Wang J,Tolan DR,Pagliaro L

更新日期：1997-12-15 00:00:00

abstract：:JANEX-1/WHI-P131, a selective Janus kinase 3 (JAK3) inhibitor, has been shown to delay the onset of diabetes in the NOD mouse model. However, the molecular mechanism by which JANEX-1 protects pancreatic beta-cells is unknown. In the current study, we investigated the role of JANEX-1 on interleukin (IL)-1beta and inter...

journal_title：Experimental cell research

authors： Lv N,Kim EK,Song MY,Choi HN,Moon WS,Park SJ,Park JW,Kwon KB,Park BH

更新日期：2009-07-15 00:00:00

abstract：:The replication kinetics and cytological changes of normal human oral keratinocytes (NHOK) isolated from the basal surface of oral epithelial sheet and cultured as dispersed cells in low (0.15 mM) Ca(2+) medium without serum were analyzed. Replicating NHOK were quantitated by cell count and identified by [(3)H]thymidi...

journal_title：Experimental cell research

authors： Kang MK,Bibb C,Baluda MA,Rey O,Park NH

更新日期：2000-08-01 00:00:00

abstract：:Integrin beta 4 subunit is present in association with alpha 6 chain on both normal and transformed epithelial cells. Recently alpha 6 beta 4 heterodimer was found on the endothelium of medium-sized blood vessels and on immature thymocytes. In this report we show, by Northern blotting, indirect immunofluorescence, imm...

journal_title：Experimental cell research

authors： Scardigli R,Soddu S,Falcioni R,Crescenzi M,Cimino L,Sacchi A

更新日期：1996-09-15 00:00:00

abstract：:There are a number of low-abundance small nuclear RNAs (snRNAs) in eukaryotic cells. Many of them have been assigned functions in the biogenesis of cellular RNAs, such as splicing and 3' end processing. Here, we present the sequence of Xenopus U12 snRNA and compare the secondary structures of the low-abundance U11 and...

journal_title：Experimental cell research

authors： Yu YT,Tarn WY,Yario TA,Steitz JA

更新日期：1996-12-15 00:00:00

abstract：:Transfection of primary rat keratinocytes with the polyomavirus large T gene promotes the establishment of cell lines. The keratinocytes express the large T protein and can be continuously cultured in medium containing a low concentration of calcium. The immortalized keratinocytes retain the ability to differentiate w...

journal_title：Experimental cell research

authors： Bayley SA,Stones AJ,Smith CG

更新日期：1988-07-01 00:00:00

abstract：:Mutations in PKHD1 (polycystic kidney and hepatic disease gene 1) gene cause the autosomal recessive polycystic kidney disease (ARPKD). Fibrocystin/polyductin (FPC), encoded by PKHD1, is a membrane-associated receptor-like protein. Although it is widely accepted that cystogenesis is mostly due to aberrant cell prolife...

journal_title：Experimental cell research

authors： Sun L,Wang S,Hu C,Zhang X

更新日期：2011-04-15 00:00:00

abstract：:The phenotypic transition of smooth muscle cells (SMC) from a contractile to a synthetic state appears to be an early event in the pathogenesis of atherosclerosis. We examined the effects of extracellular matrix components on the phenotypic modulation of rabbit arterial SMC in primary culture by flow cytometry. The re...

journal_title：Experimental cell research

authors： Yamamoto M,Yamamoto K,Noumura T

更新日期：1993-01-01 00:00:00

abstract：:3-phosphoinositide-dependent kinase-1 (PDK1) phosphorylates and activates several kinases in the cAMP-dependent, cGMP-dependent and protein kinase C (AGC) family. Many putative PDK1 substrates have been identified, but have not been analyzed following transient and specific inhibition of PDK1 activity. Here, we demons...

journal_title：Experimental cell research

authors： Tamgüney T,Zhang C,Fiedler D,Shokat K,Stokoe D

更新日期：2008-07-01 00:00:00