Abstract：
:FTO, a demethylase for N6-methyladenosine (m6A) modification, has been implicated in multiple tumors. However, its roles in esophageal squamous cell carcinoma (ESCC) remain uncovered. This study aims to evaluate the clinical relevance and functional roles in this disease. Through immunohistochemistry, qRT-PCR and Western blot analyses, we found FTO expression in ESCC tissues was stronger than that in adjacent normal tissues, and the survival curves displayed high FTO expression had a trend toward poor prognosis. Functionally, silencing of FTO inhibited ESCC cell growth and migration in CCK8, EdU, colony formation and transwell assays and FTO overexpression showed the opposite results. Furthermore, FTO was also required for the tumorigenicity of ESCC cells in nude mice. The data from RNA-seq analysis revealed that MMP13 expression was significantly affected by FTO knockdown. qRT-PCR and Western blot assays confirmed that MMP13 was positively regulated by FTO in both mRNA and protein levels. Additionally, the functional link between FTO and MMP13 was explored by CCK8 and transwell chamber approaches. These findings suggest that FTO is up-regulated and plays oncogenic roles in ESCC. MMP13 may function as a downstream target of FTO.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Liu S,Huang M,Chen Z,Chen J,Chao Q,Yin X,Quan Mdoi
10.1016/j.yexcr.2020.111894subject
Has Abstractpub_date
2020-04-01 00:00:00pages
111894issue
1eissn
0014-4827issn
1090-2422pii
S0014-4827(20)30093-8journal_volume
389pub_type
杂志文章abstract：:To measure the adhesion of cells expressing the neural cell adhesion molecule N-CAM, mouse Lmtk- fibroblast cells were transfected by a calcium phosphate precipitation technique with eucaryotic expression vectors encoding N-CAM polypeptides. We obtained cell lines expressing the 140-kDa transmembrane isoform of N-CAM ...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1993.1041
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abstract：:We have previously demonstrated that pretreatment of several cell lines with cis-unsaturated fatty acids, like oleic acid, blocks epidermal growth factor (EGF)-induced early ionic signals, and in particular the [Ca2+]i rise. In the present work we show that this blockade does not alter EGF-stimulated cellular prolifer...
journal_title：Experimental cell research
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journal_title：Experimental cell research
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2004.04.009
更新日期：2004-08-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章,评审
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更新日期：2016-04-10 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(92)90373-g
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2012.12.003
更新日期：2013-02-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(85)90140-5
更新日期：1985-04-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2008.10.007
更新日期：2009-01-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(78)90368-3
更新日期：1978-05-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：1997-07-10 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2018.05.019
更新日期：2018-08-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2007.02.016
更新日期：2007-05-01 00:00:00
abstract：:The protein composition of the nuclear matrix of murine P19 embryonal carcinoma (EC) cells was compared with that of clonal derivatives of P19 EC differentiated in vitro, and with that of P19 EC cells induced to differentiate with retinoic acid (RA). Several major differences in nuclear matrix protein composition were...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(89)90072-4
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journal_title：Experimental cell research
pub_type： 杂志文章
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journal_title：Experimental cell research
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journal_title：Experimental cell research
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journal_title：Experimental cell research
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/j.yexcr.2007.03.013
更新日期：2007-05-15 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：2003-07-01 00:00:00
