FTO promotes cell proliferation and migration in esophageal squamous cell carcinoma through up-regulation of MMP13.


:FTO, a demethylase for N6-methyladenosine (m6A) modification, has been implicated in multiple tumors. However, its roles in esophageal squamous cell carcinoma (ESCC) remain uncovered. This study aims to evaluate the clinical relevance and functional roles in this disease. Through immunohistochemistry, qRT-PCR and Western blot analyses, we found FTO expression in ESCC tissues was stronger than that in adjacent normal tissues, and the survival curves displayed high FTO expression had a trend toward poor prognosis. Functionally, silencing of FTO inhibited ESCC cell growth and migration in CCK8, EdU, colony formation and transwell assays and FTO overexpression showed the opposite results. Furthermore, FTO was also required for the tumorigenicity of ESCC cells in nude mice. The data from RNA-seq analysis revealed that MMP13 expression was significantly affected by FTO knockdown. qRT-PCR and Western blot assays confirmed that MMP13 was positively regulated by FTO in both mRNA and protein levels. Additionally, the functional link between FTO and MMP13 was explored by CCK8 and transwell chamber approaches. These findings suggest that FTO is up-regulated and plays oncogenic roles in ESCC. MMP13 may function as a downstream target of FTO.


Exp Cell Res


Liu S,Huang M,Chen Z,Chen J,Chao Q,Yin X,Quan M




Has Abstract


2020-04-01 00:00:00














  • The effects of activin A on the migration of human breast cancer cells and neutrophils and their migratory interaction.

    abstract::Activin A belongs to the superfamily of transforming growth factor beta (TGFβ) and is a critical regulatory cytokine in breast cancer and inflammation. However, the role of activin A in migration of breast cancer cells and immune cells was not well characterized. Here, a microfluidic device was used to examine the eff...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Xie D,Liu Z,Wu J,Feng W,Yang K,Deng J,Tian G,Santos S,Cui X,Lin F

    更新日期:2017-08-01 00:00:00

  • MicroRNA-182 inhibits HCMV replication through activation of type I IFN response by targeting FOXO3 in neural cells.

    abstract::Human cytomegalovirus (HCMV) has led to kinds of clinical disorders and great morbidity worldwide, such as sensorineural hearing loss (SNHL), mental retardation, and developmental delays in immunocompromised individuals. Congenital HCMV infection is a leading cause of birth defects, primarily manifesting as neurologic...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: He X,Teng J,Cui C,Li D,Wen L

    更新日期:2018-08-15 00:00:00

  • Head-to-head association in bovine spermatozoa induced by catecholamines.

    abstract::Noradrenaline, adrenaline, and isoproterenol induce head-to-head association in bovine spermatozoa in a Tris-HCl-buffered medium. Noradrenaline was the most and isoproterenol the least efficient. This effect was stimulated by Ca2+, Mg2+ and Mn2+, Ca2+ being more efficient than the other two ions. At 2 X 10(-6) M Ca2+,...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Lindahl PE

    更新日期:1978-05-01 00:00:00

  • Interleukin-1 differentially modulates chondrocyte expression of cyclooxygenase-2 and phospholipase A2.

    abstract::Interleukin-1 beta (IL-1) increases the synthesis of prostaglandins as well as the expression of synovial fluid phospholipase A2 (PLA2) mRNA and activity by chondrocytes. In order to examine the potential involvement of cyclooxygenase in the induction of prostaglandins by IL-1, the effect of IL-1 on rabbit articular c...

    journal_title:Experimental cell research

    pub_type: 杂志文章


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    更新日期:1993-05-01 00:00:00

  • Intranuclear distribution of SV40 large T-antigen and transformation-related protein p53 in abortively infected cells.

    abstract::The intranuclear localization of SV40 T-antigen (T-Ag) and the cellular protein p53 was studied in SV40 abortively infected baby mouse kidney cells using two complementary methods of ultrastructural immunocytochemistry in combination with preferential staining of nuclear RNP components and electron microscope autoradi...

    journal_title:Experimental cell research

    pub_type: 杂志文章


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  • pH in the endosome. Measurements during pinocytosis and receptor-mediated endocytosis.

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    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Geisow MJ,Evans WH

    更新日期:1984-01-01 00:00:00

  • Expression of the first N-glycosylation gene in the dolichol pathway, ALG7, is regulated at two major control points in the G1 phase of the Saccharomyces cerevisiae cell cycle.

    abstract::The Saccharomyces cerevisiae ALG7 gene, which functions by initiating the dolichol pathway of protein N-glycosylation, displays properties of an early growth-response gene. To initiate studies of the involvement of ALG7 in cellular proliferation, we have now more precisely analyzed ALG7 expression in the G1 phase of c...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Pretel R,Lennon K,Bird A,Kukuruzinska MA

    更新日期:1995-08-01 00:00:00

  • Effects of prostaglandin E2 on the subcellular localization of Epac-1 and Rap1 proteins during Fcgamma-receptor-mediated phagocytosis in alveolar macrophages.

    abstract::Recent studies have demonstrated a central role for the exchange protein activated by cAMP (Epac) in the inhibition of Fcgamma-receptor-mediated phagocytosis and bacterial killing by prostaglandin E(2) (PGE(2)) in macrophages. However, the subcellular localization of Epac, and its primary target Rap1, has yet to be de...

    journal_title:Experimental cell research

    pub_type: 杂志文章


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    更新日期:2008-01-15 00:00:00

  • Stromal cell-derived factor-1alpha (SDF-1alpha/CXCL12) stimulates ovarian cancer cell growth through the EGF receptor transactivation.

    abstract::Ovarian cancer (OC) is the leading cause of death in gynecologic diseases in which there is evidence for a complex chemokine network. Chemokines are a family of proteins that play an important role in tumor progression influencing cell proliferation, angiogenic/angiostatic processes, cell migration and metastasis, and...

    journal_title:Experimental cell research

    pub_type: 杂志文章


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    更新日期:2005-08-15 00:00:00

  • The von Hippel-Lindau tumor suppressor protein influences microtubule dynamics at the cell periphery.

    abstract::The von Hippel-Lindau (VHL) protein protects microtubules (MTs) from destabilization by nocodazole treatment. Based on this fixed-cell assay with static end points, VHL has been reported to directly stabilize the MT cytoskeleton. To investigate the dynamic changes in MTs induced by VHL in living cells, we measured the...

    journal_title:Experimental cell research

    pub_type: 杂志文章


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    更新日期:2004-12-10 00:00:00

  • Trefoil peptides promote restitution of wounded corneal epithelial cells.

    abstract::The ocular surface shares many characteristics with mucosal surfaces. In both, healing is regulated by peptide growth factors, cytokines, and extracellular matrix proteins. However, these factors are not sufficient to ensure most rapid healing. Trefoil peptides are abundantly expressed epithelial cell products which e...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Göke MN,Cook JR,Kunert KS,Fini ME,Gipson IK,Podolsky DK

    更新日期:2001-04-01 00:00:00

  • Cell-cell contact promotes specific activity of pp60c-src protein kinase in human retinoblastoma cells.

    abstract::Tyrosine-specific protein kinase activity of pp60c-src was examined in human Y79 retinoblastoma cells cultured in monolayers after clusters in suspension culture had been dissociated. The activity increased five- to six-fold between Days 1 and 7 in the monolayer cultures, with a concomitant increase in numbers of cell...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Kato G

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  • MiR-212-3p inhibits LPS-induced inflammatory response through targeting HMGB1 in murine macrophages.

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    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Chen W,Ma X,Zhang P,Li Q,Liang X,Liu J

    更新日期:2017-01-15 00:00:00

  • Interaction of methylmercury with microtubules in cultured cells and in vitro.

    abstract::The effects of methylmercury (MeHg) on cytoplasmic microtubules in cultured fibroblasts and on the in vitro polymerization of microtubules were examined. MeHg caused disruption of cellular microtubules in a concentration- and time-dependent manner. Addition of the metal-chelating agent, dimercaptosuccinic acid (DMSA),...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Sager PR,Doherty RA,Olmsted JB

    更新日期:1983-06-01 00:00:00

  • Regulation of pyruvate kinase expression and growth in mastocytoma cells. I. Initial observations.

    abstract::The specific activities of pyruvate kinase and phosphofructokinase but not lactate dehydrogenase increase as P-815 mastocytoma cells approach the stationary phase. During this growth period, the rates of uptake of labelled precursors into DNA, RNA and total protein decreases. On the other hand, the pyruvate kinase pro...

    journal_title:Experimental cell research

    pub_type: 杂志文章


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    更新日期:1983-12-01 00:00:00

  • Identification of the GTPase-activating protein DEP domain containing 1B (DEPDC1B) as a transcriptional target of Pitx2.

    abstract::Pitx2 is a bicoid-related homeobox transcription factor implicated in regulating left-right patterning and organogenesis. However, only a limited number of Pitx2 downstream target genes have been identified and characterized. Here we demonstrate that Pitx2 is a transcriptional repressor of DEP domain containing 1B (DE...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Wu D,Zhu X,Jimenez-Cowell K,Mold AJ,Sollecito CC,Lombana N,Jiao M,Wei Q

    更新日期:2015-04-10 00:00:00

  • The cytoplasmic pH, ATP content and total protein synthesis rate during heat-shock protein inducing treatments in yeast.

    abstract::In S. cerevisiae the induction of heat-shock protein (HSP) synthesis is accompanied by a decrease in the cytoplasmic and vacuolar pH as determined by means of [31P]NMR spectroscopy. The relationship of HSP synthesis and acidification of the cytoplasmic pH is dose-dependent under a variety of treatments (temperature in...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Weitzel G,Pilatus U,Rensing L

    更新日期:1987-05-01 00:00:00

  • Hepatocyte growth factor enhances adhesion of breast cancer cells to endothelial cells in vitro through up-regulation of CD44.

    abstract::For cancer metastasis, tumor cells present in the circulation must first adhere to the endothelium. Integrins play a central role in leukocyte adhesion to the endothelium and subsequent migration into tissues. The majority of tumor cells derived from solid cancers, including breast cancer, do not express integrins. We...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Mine S,Fujisaki T,Kawahara C,Tabata T,Iida T,Yasuda M,Yoneda T,Tanaka Y

    更新日期:2003-08-01 00:00:00

  • Adriamycin promotes neurite outgrowth in the "neurite-minus" N1A-103 mouse neuroblastoma cell line.

    abstract::Adriamycin, an anticancer agent acting on topoisomerase II, promotes the arrest of cell division and neurite extension in a "neurite-minus" murine neuroblastoma cell line, N1A-103. This morphological differentiation is accompanied by a blockade in the S phase of the cell cycle, modification of the amount of peripherin...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Larcher JC,Cordeau-Lossouarn L,Romey G,Gros F,Croizat B,Vayssiere JL

    更新日期:1992-11-01 00:00:00

  • Immunodetection of poly(A) binding protein II in the cell nucleus.

    abstract::During the polyadenylation of pre-mRNA in vitro, poly(A) binding protein II (PAB II) binds to the growing poly(A) tail, stimulating its extension. The subcellular localization of PAB II was investigated with an antibody affinity-purified from rabbit serum raised against the purified protein. Immunofluorescence microsc...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Krause S,Fakan S,Weis K,Wahle E

    更新日期:1994-09-01 00:00:00

  • Integrity of actin fibers and microtubules influences metastatic tumor cell adhesion.

    abstract::Tumor cell adhesion within host organ microvasculature, its stabilization and invasion into host organ parenchyma appear to be important steps during formation of distant metastasis. These interactions of circulating tumor cells with the host organs occur in the presence of fluid shear forces and soluble and cellular ...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Korb T,Schlüter K,Enns A,Spiegel HU,Senninger N,Nicolson GL,Haier J

    更新日期:2004-09-10 00:00:00

  • Vascular smooth muscle cell detachment from elastin and migration through elastic laminae is promoted by chondroitin sulfate-induced "shedding" of the 67-kDa cell surface elastin binding protein.

    abstract::Impaired elastin fiber assembly is observed in the fetal ductus arteriosus (DA), associated with a reduced concentration of elastin binding protein (EBP), a 67-kDa galactolectin. It is also seen in cultured aortic (Ao) smooth muscle cells (SMC) following the release of the EBP by glycosaminoglycans rich in N-acetylgal...

    journal_title:Experimental cell research

    pub_type: 杂志文章


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  • Rictor-targeting exosomal microRNA-16 ameliorates lung fibrosis by inhibiting the mTORC2-SPARC axis.

    abstract::Idiopathic pulmonary fibrosis (IPF), a progressive disorder of unknown etiology, is characterized by pathological lung fibroblast activation and proliferation resulting in abnormal deposition of extracellular matrix proteins within the lung parenchyma. The pathophysiological roles of exosomal microRNAs in pulmonary fi...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Inomata M,Kamio K,Azuma A,Matsuda K,Usuki J,Morinaga A,Tanaka T,Kashiwada T,Atsumi K,Hayashi H,Fujita K,Saito Y,Kubota K,Seike M,Gemma A

    更新日期:2021-01-15 00:00:00

  • Conventional protein kinase C mediates phorbol-dibutyrate-induced cytoskeletal remodeling in a7r5 smooth muscle cells.

    abstract::Phorbol dibutyrate (PDBu) induced the formation of podosome-like structures together with partial disassembly of actin stress fibers in A7r5 smooth muscle cells. These podosomes contained alpha-actinin, F-actin, and vinculin and exhibit a tubular, column-like structure arising perpendicularly from the bottom of PDBu-t...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Hai CM,Hahne P,Harrington EO,Gimona M

    更新日期:2002-10-15 00:00:00

  • An investigation of adhesion and detachment in slime mould amoebae using columns of hydrophobic beads.

    abstract::Vegetative amoebae of Dictyostelium discoideum (strain AX2) adhere to columns of hydrophobic beads (Bio-Beads SM-2) which have been coated with axenic growth medium. This binding is blocked by sodium azide and other inhibitors of aerobic respiration. Bound cells are not detached by lowering the temperature but they ca...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Glynn PJ,Clarke KR

    更新日期:1984-05-01 00:00:00

  • Induction of M-phase entry of prophase-blocked mouse oocytes through microinjection of okadaic acid, a specific phosphatase inhibitor.

    abstract::We report that a specific inhibitor of types 1 and 2A phosphatases, okadaic acid (OA), induces germinal vesicle break down (GVBD) and chromosome condensation when microinjected into denuded mouse oocytes maintained in prophase block by analogs of cAMP, inhibitors of phosphodiesterase, or a tumor-promoting phorbol este...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Gavin AC,Tsukitani Y,Schorderet-Slatkine S

    更新日期:1991-01-01 00:00:00

  • Homocysteine regulates expression of Herp by DNA methylation involving the AARE and CREB binding sites.

    abstract::Herp (homocysteine-induced endoplasmic reticulum protein) is an ER-resident membrane protein, which has a ubiquitin-like domain at its N-terminus. Expression of Herp protein is up-regulated in response to ER stress, including homocysteine. Herp stabilizes neuronal Ca(2+) homeostasis and is involved in improving the ba...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Lenz B,Bleich S,Beutler S,Schlierf B,Schwager K,Reulbach U,Kornhuber J,Bönsch D

    更新日期:2006-12-10 00:00:00

  • Mechanical regulation of myofibroblast phenoconversion and collagen contraction.

    abstract::Activated fibroblasts promote physiological wound repair following tissue injury. However, dysregulation of fibroblast activation contributes to the development of fibrosis by enhanced production and contraction of collagen-rich extracellular matrix. At the peak of their activities, fibroblasts undergo phenotypic conv...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Hinz B,McCulloch CA,Coelho NM

    更新日期:2019-06-01 00:00:00

  • p120 serine and threonine phosphorylation is controlled by multiple ligand-receptor pathways but not cadherin ligation.

    abstract::p120-catenin (p120) regulates cadherin turnover and is required for cadherin stability. Extensive and dynamic phosphorylation on tyrosine, serine and threonine residues in the N-terminal regulatory domain has been postulated to regulate p120 function, possibly through modulation of the efficiency of p120/cadherin inte...

    journal_title:Experimental cell research

    pub_type: 杂志文章


    authors: Xia X,Carnahan RH,Vaughan MH,Wildenberg GA,Reynolds AB

    更新日期:2006-10-15 00:00:00

  • Liver 'organ on a chip'.

    abstract::The liver plays critical roles in both homeostasis and pathology. It is the major site of drug metabolism in the body and, as such, a common target for drug-induced toxicity and is susceptible to a wide range of diseases. In contrast to other solid organs, the liver possesses the unique ability to regenerate. The phys...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审


    authors: Beckwitt CH,Clark AM,Wheeler S,Taylor DL,Stolz DB,Griffith L,Wells A

    更新日期:2018-02-01 00:00:00