当前位置： SCI文献检索 > EXPERIMENTAL CELL RESEARCH期刊下所有文献 > Type I collagen promotes modulation of cultured rabbit arterial smooth muscle cells from a contractile to a synthetic phenotype.

Type I collagen promotes modulation of cultured rabbit arterial smooth muscle cells from a contractile to a synthetic phenotype.

Abstract：

:The phenotypic transition of smooth muscle cells (SMC) from a contractile to a synthetic state appears to be an early event in the pathogenesis of atherosclerosis. We examined the effects of extracellular matrix components on the phenotypic modulation of rabbit arterial SMC in primary culture by flow cytometry. The results demonstrate that freshly isolated SMC attached, spread, and started to proliferate on type I collagen as well as on fibronectin. Moreover, type I collagen was as efficient as fibronectin in promoting the transition of the cells into the synthetic phenotype without exogenous mitogens. However, unlike on fibronectin, the synthetic peptide GRGDSP (Gly-Arg-Gly-Asp-Ser-Pro) and the peptide KDGEA (Lys-Asp-Gly-Glu-Ala), which contains the recognition sequence for alpha 2 beta 1 integrin in type I collagen, interfered little with the attachment, spreading, and phenotypic modulation of the cells on type I collagen. On the other hand, the phenotypic modulation of the cells was counteracted by the anti-beta 1 integrin antibody. These findings indicate that type I collagen promotes the phenotypic transition of the rabbit arterial SMC by interacting with a cell surface receptor (beta 1 integrin family) for a cell-binding sequence without RGD and DGEA. In contrast, elastin, a major constituent of the media, suppressed the cell attachment and spreading and maintained the cells in the contractile phenotype as laminin. These results suggest diverse roles of type I collagen and elastin as well as of fibronectin and laminin in the control of the differentiated properties of arterial SMC.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Yamamoto M,Yamamoto K,Noumura T

doi

10.1006/excr.1993.1016

subject

Has Abstract

pub_date

1993-01-01 00:00:00

pages

121-9

issue

1

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(83)71016-5

journal_volume

204

pub_type

杂志文章

相关文献

EXPERIMENTAL CELL RESEARCH文献大全
  • Chronic kidney disease induces inflammation by increasing Toll-like receptor-4, cytokine and cathelicidin expression in neutrophils and monocytes.

    abstract：:TLR expression in neutrophils and monocytes is associated with increased cytokine synthesis, resulting in increased inflammation. However, the inflammatory pathway related to TLR and cathelicidin expression in these cells from CKD patients is unclear. To evaluate TLR4, cathelicidin, TNF-α, IL-6, IL-10 and MCP-1 expres...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2018.02.022

    authors： Grabulosa CC,Manfredi SR,Canziani ME,Quinto BMR,Barbosa RB,Rebello JF,Batista MC,Cendoroglo M,Dalboni MA

    更新日期：2018-04-15 00:00:00

  • Internalization of the hyaluronan receptor CD44 by chondrocytes.

    abstract：:Chondrocytes express CD44 as a primary receptor for the matrix macromolecule hyaluronan. Hyaluronan is responsible for the retention and organization of proteoglycan within cartilage, and hyaluronan-chondrocyte interactions are important for the assembly and maintenance of the cartilage matrix. Bovine articular chondr...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1999.4641

    authors： Aguiar DJ,Knudson W,Knudson CB

    更新日期：1999-11-01 00:00:00

  • Ruta graveolens water extract inhibits cell-cell network formation in human umbilical endothelial cells via MEK-ERK1/2 pathway.

    abstract：:Angiogenesis is a process encompassing several steps such as endothelial cells proliferation, differentiation and migration to form a vascular network, involving different signal transduction pathways. Among these, ERK1/2 signaling mediates VEGF-dependent signaling pathway. Here we report that the water extract of Rut...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2018.01.025

    authors： Gentile MT,Russo R,Pastorino O,Cioffi S,Barbieri F,Illingworth EA,Grieco M,Chambery A,Colucci-D'Amato L

    更新日期：2018-03-01 00:00:00

  • Tumor exosomes induce tunneling nanotubes in lipid raft-enriched regions of human mesothelioma cells.

    abstract：:Tunneling nanotubes (TnTs) are long, non-adherent, actin-based cellular extensions that act as conduits for transport of cellular cargo between connected cells. The mechanisms of nanotube formation and the effects of the tumor microenvironment and cellular signals on TnT formation are unknown. In the present study, we...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2014.01.014

    authors： Thayanithy V,Babatunde V,Dickson EL,Wong P,Oh S,Ke X,Barlas A,Fujisawa S,Romin Y,Moreira AL,Downey RJ,Steer CJ,Subramanian S,Manova-Todorova K,Moore MAS,Lou E

    更新日期：2014-04-15 00:00:00

  • Migration-stimulating factor (MSF) is over-expressed in non-small cell lung cancer and promotes cell migration and invasion in A549 cells over-expressing MSF.

    abstract：:Migration-stimulating factor (MSF), an oncofetal truncated isoform of fibronectin, is a potent stimulator of cell invasion. However, its distribution and motogenic role in non-small cell lung cancer (NSCLC) have never been identified. In this study, real-time PCR and immunohistochemical staining (IHC) were performed t...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2013.05.016

    authors： Deng X,Ma Q,Zhang B,Jiang H,Zhang Z,Wang Y

    更新日期：2013-10-15 00:00:00

  • Dynamic reorganization of chemokine receptors, cholesterol, lipid rafts, and adhesion molecules to sites of CD4 engagement.

    abstract：:T cell polarization and redistribution of cellular components are critical to processes such as activation, migration, and potentially HIV infection. Here, we investigate the effects of CD4 engagement on the redistribution and localization of chemokine receptors, CXCR4 and CCR5, adhesion molecules, and lipid raft comp...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2004.11.022

    authors： Nguyen DH,Giri B,Collins G,Taub DD

    更新日期：2005-04-01 00:00:00

  • Induction of endothelial cell migration by proline analogs and its relevance to angiogenesis.

    abstract：:The proline analogs cis-4-hydroxy-L-proline, 3,4-dehydro-L-proline, and 2-azetidinecarboxylic acid induced increases in the migration rate of retinal capillary endothelial cells, aortal endothelial cells, corneal endothelial cells, aortal smooth muscle cells, and retinal pericytes. cis-4-hydroxy-D-proline did not. The...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(88)90328-x

    authors： McAuslan BR,Reilly W,Hannan GN,Schindhelm K,Milthorpe B,Saur BA

    更新日期：1988-06-01 00:00:00

  • Effects of polyamines on the first division cycle of Xenopus laevis eggs.

    abstract：:The involvement of polyamines in cytokinesis has been examined in eggs of the amphibian X. laevis. Microinjection of spermine or spermidine into unfertilized eggs induced a shortening of the first division cycle and early formation of cleavage-like constrictions. Eggs were activated by injection and developed furrows ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(84)90593-7

    authors： Grant NJ,Aimar C,Oriol-Audit C

    更新日期：1984-02-01 00:00:00

  • Neddylation-mediated Nedd4-2 activation regulates ubiquitination modification of renal NBCe1.

    abstract：:The sodium-coupled bicarbonate cotransporter 1 (NBCe1) plays an essential role in the maintenance of acid-base homeostasis in the human body. However, little research has been done regarding the modification of NBCe1. Nedd4-2 is one of the most important ubiquitin E3 ligases in the kidney where it is responsible for m...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2020.111958

    authors： Tu J,Zhang B,Fang G,Chang W,Zhao Y

    更新日期：2020-05-15 00:00:00

  • Role of the F-actin cytoskeleton in the RVD and RVI processes in Ehrlich ascites tumor cells.

    abstract：:The role of the F-actin cytoskeleton in cell volume regulation was studied in Ehrlich ascites tumor cells, using a quantitative rhodamine-phalloidin assay, confocal laser scanning microscopy, and electronic cell sizing. A hypotonic challenge (160 mOsm) was associated with a decrease in cellular F-actin content at 1 an...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1999.4615

    authors： Pedersen SF,Mills JW,Hoffmann EK

    更新日期：1999-10-10 00:00:00

  • Cytosolic free calcium spiking affected by intracellular pH change.

    abstract：:The characteristics underlying cytosolic free calcium oscillation were evaluated by superfused dual wave-length microspectrofluorometry of fura-2-loaded single acinar cells from rat pancreas. Application of a physiological concentration of cholecystokinin octapeptide (CCK) (20 pM) induced a small basal increase in cyt...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(90)90173-8

    authors： Tsunoda Y

    更新日期：1990-06-01 00:00:00

  • A mutant allele of BARA/LIN-9 rescues the cdk4-/- phenotype by releasing the repression on E2F-regulated genes.

    abstract：:It has been proposed that C. elegans LIN-9 functions downstream of CDK4 in a pathway that regulates cell proliferation. Here, we report that mammalian BARA/LIN-9 is a predominantly nuclear protein that inhibits cell proliferation. More importantly, we demonstrate that BARA/LIN-9 also acts downstream of cyclin D/CDK4 i...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2006.04.002

    authors： Sandoval R,Xue J,Tian X,Barrett K,Pilkinton M,Ucker DS,Raychaudhuri P,Kineman RD,Luque RM,Baida G,Zou X,Valli VE,Cook JL,Kiyokawa H,Colamonici OR

    更新日期：2006-08-01 00:00:00

  • TNF-alpha and 9-cis-retinoic acid synergistically induce ICAM-1 expression: evidence for interaction of retinoid receptors with NF-kappa B.

    abstract：:TNF-alpha and 9-cis-retinoic acid (9-cis-R) synergistically enhance ICAM-1 protein expression in immortalized human aortic endothelial cells (HAECTs). At a TNF-alpha concentration of 0.1 ng/ml, 1 microM 9-cis-R enhanced ICAM-1 protein expression 4-fold. Treatment with 1 microM 9-cis-R alone caused no induction of ICAM...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1997.3913

    authors： Chadwick CC,Shaw LJ,Winneker RC

    更新日期：1998-03-15 00:00:00

  • Plakophilin3 loss leads to an increase in lipocalin2 expression, which is required for tumour formation.

    abstract：:An increase in tumour formation and metastasis are observed upon plakophilin3 (PKP3) loss. To identify pathways downstream of PKP3 loss that are required for increased tumour formation, a gene expression analysis was performed, which demonstrated that the expression of lipocalin2 (LCN2) was elevated upon PKP3 loss and...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2018.05.026

    authors： Basu S,Chaudhary N,Shah S,Braggs C,Sawant A,Vaz S,Thorat R,Gupta S,Dalal SN

    更新日期：2018-08-15 00:00:00

  • Fibroblast growth factors 1 and 2 inhibit adipogenesis of human bone marrow stromal cells in 3D collagen gels.

    abstract：:Multipotent human bone marrow stromal cells (hBMSCs) are the common progenitors of osteoblasts and adipocytes. A shift in hBMSC differentiation in favor of adipogenesis may contribute to the bone loss and marrow fat accumulation observed in aging and osteoporosis. Hence, the identification of factors modulating marrow...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2015.09.009

    authors： Le Blanc S,Simann M,Jakob F,Schütze N,Schilling T

    更新日期：2015-11-01 00:00:00

  • Cytofluorometric DNA base determination for the investigation of heterochromatin and heterochromatin amplification.

    abstract：:The measurement of chromomycin A3/DAPI fluorescence ratios is shown to allow base content determinations in eu- and heterochromatic regions of interphase cell nuclei. The base content values obtained in chromocenters and euchromatin of Scilla sibirica agree with those measured earlier [12] on the band and non-band are...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(83)90223-9

    authors： Leemann U,Ruch F

    更新日期：1983-09-01 00:00:00

  • Syndecan-1 ectodomain regulates matrix-dependent signaling in human breast carcinoma cells.

    abstract：:Syndecan-1 was overexpressed in T47D, MCF-7, or Hs578t human breast carcinoma cell lines, mimicking overexpression observed in carcinomas in vivo. Overexpression of syndecan-1, or its ectodomain alone fused to a glycosylphosphatidylinositol anchor (GPI-mS1ED), promotes cell rounding in 2D culture. Deletions within the...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2004.07.001

    authors： Burbach BJ,Ji Y,Rapraeger AC

    更新日期：2004-10-15 00:00:00

  • In situ hybridization in living cells: detection of RNA molecules.

    abstract：:Fluorescence hybridization is a widely used technique in cell biology and pathology for detecting specific nucleic acid (DNA and RNA) sequences in fixed cells. This technique does not, however, provide dynamic information on the intracellular behavior of the targeted molecules. The aim of this work was to investigate ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1996.3464

    authors： Paillasson S,Van De Corput M,Dirks RW,Tanke HJ,Robert-Nicoud M,Ronot X

    更新日期：1997-02-25 00:00:00

  • Mononuclear cell-mediated modulation of synovial cell metabolism. I. Collagen synthesis.

    abstract：:Human PHA-stimulated mononuclear cells produce a factor which inhibits synovial cell collagen and non-collagen protein synthesis, whereas it enhances hyaluronic acid (HA) production. Indomethacin (10(-4)-10(-6) M), a cyclo-oxygenase inhibitor, suppresses this effect, suggesting that the mechanism is prostaglandin-medi...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(85)90431-8

    authors： Pujol JP,Penfornis H,Arenzana-Seisdedos F,Bocquet J,Farjanel J,Rattner A,Brisset M,Virelizier JL,Beliard R,Loyau G

    更新日期：1985-05-01 00:00:00

  • Rictor-targeting exosomal microRNA-16 ameliorates lung fibrosis by inhibiting the mTORC2-SPARC axis.

    abstract：:Idiopathic pulmonary fibrosis (IPF), a progressive disorder of unknown etiology, is characterized by pathological lung fibroblast activation and proliferation resulting in abnormal deposition of extracellular matrix proteins within the lung parenchyma. The pathophysiological roles of exosomal microRNAs in pulmonary fi...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2020.112416

    authors： Inomata M,Kamio K,Azuma A,Matsuda K,Usuki J,Morinaga A,Tanaka T,Kashiwada T,Atsumi K,Hayashi H,Fujita K,Saito Y,Kubota K,Seike M,Gemma A

    更新日期：2021-01-15 00:00:00

  • Heterogeneity in distribution and content of p53 in SV40-transformed mouse fibroblasts.

    abstract：:The high level and intranuclear location of the cellular phosphoprotein p53 are usually regarded as invariant features of SV40-transformed fibroblast lines. During the development of improved methods for immunocytochemical detection of p53 using SVA31 E7 mouse fibroblasts, we have observed unexpected and marked variat...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(86)90510-0

    authors： Davis D,Wynford-Thomas D

    更新日期：1986-09-01 00:00:00

  • HRP-2, a heterogeneous nuclear ribonucleoprotein, is essential for embryogenesis and oogenesis in Caenorhabditis elegans.

    abstract：:Heterogeneous nuclear ribonucleoproteins (hnRNPs) have fundamental roles in the posttranscriptional control of gene expression. Here, we describe an hnRNP from Caenorhabditis elegans(HRP-2), which shares significant homology with mammalian hnRNP R, hnRNP Q and ACF, the essential complementation factor in ApoB mRNA edi...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2004.04.043

    authors： Kinnaird JH,Maitland K,Walker GA,Wheatley I,Thompson FJ,Devaney E

    更新日期：2004-08-15 00:00:00

  • RalGPS2 is involved in tunneling nanotubes formation in 5637 bladder cancer cells.

    abstract：:RalGPS2 is a Ras-independent Guanine Nucleotide Exchange Factor (GEF) for RalA containing a PH domain and an SH3-binding region and it is involved in several cellular processes, such as cytokinesis, control of cell cycle progression, differentiation, cytoskeleton organization and rearrangement. Up to now, few data hav...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2017.11.036

    authors： D'Aloia A,Berruti G,Costa B,Schiller C,Ambrosini R,Pastori V,Martegani E,Ceriani M

    更新日期：2018-01-15 00:00:00

  • The cell-cell adhesion molecule EpCAM interacts directly with the tight junction protein claudin-7.

    abstract：:We recently described that in the metastasizing rat pancreatic carcinoma line BSp73ASML the cell-cell adhesion molecule EpCAM, CD44 variant isoforms and the tetraspanins D6.1A and CD9 form a complex that is located in glycolipid-enriched membrane microdomains. This complex contains, in addition, an undefined 20 kDa pr...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2005.06.013

    authors： Ladwein M,Pape UF,Schmidt DS,Schnölzer M,Fiedler S,Langbein L,Franke WW,Moldenhauer G,Zöller M

    更新日期：2005-10-01 00:00:00

  • Cell condensation in chondrogenic differentiation.

    abstract：:Reduction of intercellular spaces in the areas of prospective cartilage and bone formation (precartilage condensation) precedes chondrogenesis and may represent an important step in the process of cartilage differentiation during limb skeletogenesis. We have attempted to clarify the role of the microenvironment establ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/s0014-4827(05)80067-9

    authors： Tacchetti C,Tavella S,Dozin B,Quarto R,Robino G,Cancedda R

    更新日期：1992-05-01 00:00:00

  • Functional expression of human DNA topoisomerase I and its subcellular localization in HeLa cells.

    abstract：:DNA topoisomerase (topo) I plays an important role in DNA metabolism by relieving the torsional restraints of DNA topology through ATP-independent single-strand DNA breakage. In the present study, we expressed human topo I in HeLa cells by fusing it to enhanced green fluorescent protein (EGFP). The EGFP-topo I fusion ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2000.4864

    authors： Mo YY,Wang P,Beck WT

    更新日期：2000-05-01 00:00:00

  • Aberrant miR-21 and miR-200b expression and its pro-fibrotic potential in hypertrophic scars.

    abstract：:The post-traumatic hypertrophic scar (HS) is a fibrotic disease with excessive extracellular matrix (ECM) production by fibroblasts in response to tissue injury. Although dysregulation of miRNAs is known to be involved in a variety of pathophysiologic processes, the role of miRNA in hypertrophic scar formation is uncl...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2015.10.018

    authors： Zhou R,Zhang Q,Zhang Y,Fu S,Wang C

    更新日期：2015-12-10 00:00:00

  • The cytoplasmic domain of the alpha1 integrin subunit influences stress fiber formation via the conserved GFFKR motif.

    abstract：:Integrins are heterodimeric transmembrane proteins that mediate substrate adhesion and migration but also the bidirectional transfer of information across the plasma membrane via their cytoplasmic domains. We addressed the question of whether the very short cytoplasmic tail of the alpha1 integrin subunit of alpha1beta...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2000.4831

    authors： Vossmeyer D,Kaufmann C,Löster K,Lucka L,Horstkorte R,Reutter W,Danker K

    更新日期：2000-04-10 00:00:00

  • Research progress on the effect of autophagy-lysosomal pathway on tumor drug resistance.

    abstract：:Autophagy is an intracellular degradation pathway that is highly conserved during the evolution of eukaryotes and is based on lysosome. Under nutritional deficiencies or stress, cells can clear damaged and necrotic organelles and proteins through autophagy to maintain the homeostasis of cells and organisms. Studies ha...

    journal_title：Experimental cell research

    pub_type： 杂志文章,评审

    doi：10.1016/j.yexcr.2020.111925

    authors： Li J,Liu G,Li L,Yao Z,Huang J

    更新日期：2020-04-15 00:00:00

  • Gene expression profiling in response to the histone deacetylase inhibitor BL1521 in neuroblastoma.

    abstract：:Neuroblastoma is a childhood tumor with a poor survival in advanced stage disease despite intensive chemotherapeutic regimes. The new histone deacetylase (HDAC) inhibitor BL1521 has shown promising results in neuroblastoma. Inhibition of HDAC resulted in a decrease in proliferation and metabolic activity, induction of...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2005.06.024

    authors： de Ruijter AJ,Meinsma RJ,Bosma P,Kemp S,Caron HN,van Kuilenburg AB

    更新日期：2005-10-01 00:00:00