当前位置： SCI文献检索 > EXPERIMENTAL CELL RESEARCH期刊下所有文献 > Migration-stimulating factor (MSF) is over-expressed in non-small cell lung cancer and promotes cell migration and invasion in A549 cells over-expressing MSF.

Migration-stimulating factor (MSF) is over-expressed in non-small cell lung cancer and promotes cell migration and invasion in A549 cells over-expressing MSF.

Abstract：

:Migration-stimulating factor (MSF), an oncofetal truncated isoform of fibronectin, is a potent stimulator of cell invasion. However, its distribution and motogenic role in non-small cell lung cancer (NSCLC) have never been identified. In this study, real-time PCR and immunohistochemical staining (IHC) were performed to detect MSF mRNA and protein levels in tumor tissues and matched adjacent tumor-free tissues. Furthermore, to examine the effect of MSF on invasiveness, MSF was upregulated in A549 cells. The invasiveness and viability of A549 cells were then determined using a transwell migration assay and the 3-(4,5-Dimethylthiazol-2-yl)-2,5-diphenyltetrazolium bromide (MTT) viability assays, respectively. The expression level of MSF in NSCLC tissue was markedly higher than in matched adjacent tumor-free tissue. Additionally, the level of MSF protein expression in stage III and IV NSCLC samples was higher than in stage I and II NSCLC samples. More importantly, we also demonstrated that migration and invasion of A549 cells increased substantially after upregulating MSF, although proliferation remained unchanged. Meanwhile, we found no correlation between increasing motility and invasiveness of MSF-overexpressing cells and expression levels and activities of matrix metalloprotease MMP-2 and MMP-9. Our current study shows that MSF plays a role in migration and invasion of A549 cells and suggests that MSF may be a potential biomarker of NSCLC progression.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Deng X,Ma Q,Zhang B,Jiang H,Zhang Z,Wang Y

doi

10.1016/j.yexcr.2013.05.016

subject

Has Abstract

pub_date

2013-10-15 00:00:00

pages

2545-53

issue

17

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(13)00222-X

journal_volume

319

pub_type

杂志文章

相关文献

EXPERIMENTAL CELL RESEARCH文献大全
  • TNF-alpha and 9-cis-retinoic acid synergistically induce ICAM-1 expression: evidence for interaction of retinoid receptors with NF-kappa B.

    abstract：:TNF-alpha and 9-cis-retinoic acid (9-cis-R) synergistically enhance ICAM-1 protein expression in immortalized human aortic endothelial cells (HAECTs). At a TNF-alpha concentration of 0.1 ng/ml, 1 microM 9-cis-R enhanced ICAM-1 protein expression 4-fold. Treatment with 1 microM 9-cis-R alone caused no induction of ICAM...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1997.3913

    authors： Chadwick CC,Shaw LJ,Winneker RC

    更新日期：1998-03-15 00:00:00

  • Age-dependent migratory behavior of human endothelial cells revealed by substrate microtopography.

    abstract：:Cell migration is part of many important in vivo biological processes and is influenced by chemical and physical factors such as substrate topography. Although the migratory behavior of different cell types on structured substrates has already been investigated, up to date it is largely unknown if specimen's age affec...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2018.10.008

    authors： Sales A,Picart C,Kemkemer R

    更新日期：2019-01-01 00:00:00

  • Annexin VI is a mannose-6-phosphate-independent endocytic receptor for bovine β-glucuronidase.

    abstract：:Endocytosis and transport of bovine liver β-glucuronidase to lysosomes in human fibroblasts are mediated by two receptors: the well-characterized cation-independent mannose 6-phosphate receptor (IGF-II/Man6PR) and an IGF-II/Man6PR-independent receptor, which recognizes a Ser-Trp*-Ser sequence present on the ligand. Th...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2011.05.025

    authors： Ramírez-Mata A,Michalak C,Mendoza-Hernández G,León-Del-Río A,González-Noriega A

    更新日期：2011-10-01 00:00:00

  • Signal transduction in endothelial cells by the angiogenesis inhibitor histidine-rich glycoprotein targets focal adhesions.

    abstract：:Histidine-rich glycoprotein (HRGP) is an abundant heparin-binding plasma protein. We have shown that a fragment released from the central histidine/proline-rich (His/Pro-rich) domain of HRGP blocks endothelial cell migration in vitro and vascularization and growth of murine fibrosarcoma in vivo. The minimal active HRG...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2006.04.022

    authors： Lee C,Dixelius J,Thulin A,Kawamura H,Claesson-Welsh L,Olsson AK

    更新日期：2006-08-01 00:00:00

  • DEPDC1B knockdown inhibits the development of malignant melanoma through suppressing cell proliferation and inducing cell apoptosis.

    abstract：:Malignant melanoma (MM) remains the leading cause of skin cancer related death, which has very poor prognosis because of locoregional recurrence and distant metastasis. DEPDC1B (DEP domain-containing protein 1B), has been proved to be associated with some types of malignant tumors. However, the role of DEPDC1B in MM i...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2019.03.021

    authors： Xu Y,Sun W,Zheng B,Liu X,Luo Z,Kong Y,Xu M,Chen Y

    更新日期：2019-06-01 00:00:00

  • Detection in BHK cells of a precursor form for lamin A.

    abstract：:Lamins are structural proteins found in the fibrous lamina underlining the nuclear envelope. In vitro translation of polyadenylated RNA or polysomes followed by immunoprecipitation with a serum raised against BHK nuclear matrix proteins showed that lamin A (72 kD) is synthesized as a high molecular weight precursor (7...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(85)90084-9

    authors： Dagenais A,Bibor-Hardy V,Laliberté JF,Royal A,Simard R

    更新日期：1985-12-01 00:00:00

  • Inhibitors of phosphoinositide 3-kinase cause defects in the postendocytic sorting of beta2-adrenergic receptors.

    abstract：:Phosphatidylinositol 3-kinase inhibitors have been shown to affect endocytosis or subsequent intracellular sorting in various receptor systems. Agonist-activated beta(2)-adrenergic receptors undergo desensitization by mechanisms that include the phosphorylation, endocytosis and degradation of receptors. Following endo...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2007.04.034

    authors： Awwad HO,Iyer V,Rosenfeld JL,Millman EE,Foster E,Moore RH,Knoll BJ

    更新日期：2007-07-15 00:00:00

  • Isolation and immunohistochemical characterization of two developmentally regulated highly insoluble antigens from the membrane of sinusoidal rat liver cells.

    abstract：:Using sequential extractions with buffers containing or not containing neutral detergent, two highly insoluble protein components were found in livers of 30-day-old rats. These compounds (molecular weights (MW) 52 900 and 33 200 respectively) were practically absent from livers of young rats (between 5 and 8 postnatal...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(84)90197-6

    authors： Zanetta JP,Bingen A,Dontenwill M,Vincendon G

    更新日期：1984-12-01 00:00:00

  • PKMYT1 promoted the growth and motility of hepatocellular carcinoma cells by activating beta-catenin/TCF signaling.

    abstract：:Over-activation of beta-catenin/TCF signaling has been frequently observed in hepatocellular carcinoma (HCC). Better understanding the molecular mechanism for the aberrant activation of beta-catenin/TCF signaling would provide novel insights into the treatment of this malignancy. In this study, we have shown that the ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2017.06.014

    authors： Liu L,Wu J,Wang S,Luo X,Du Y,Huang D,Gu D,Zhang F

    更新日期：2017-09-15 00:00:00

  • Productive transcription of miR-124-3p by RelA and RNA polymerase II directs RIP1 ubiquitination-dependent apoptosis resistance during hypoxia.

    abstract：:The K63-linked ubiquitination of RIP1 coordinates survival/death homeostasis by driving transcription of genes downstream of RelA. Previously, we demonstrated that EGF-dependent RelA transactivation overcomes hypoxia-initiated apoptosis, yet the underlying mechanisms remain mysterious. We report here that UBXN1 defici...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2019.03.004

    authors： Song YK,Hu BC,Xu L,Liu JQ,Chen X,Zheng Y,Chen MH,Wang JZ,Sun RH,Mo SJ

    更新日期：2019-05-01 00:00:00

  • Induction of apoptosis in chronic lymphocytic leukemia cells and its prevention by phorbol ester.

    abstract：:Chronic lymphocytic leukemia lymphocytes were used to study mechanisms involved in apoptosis (programmed cell death). Apoptosis, which was determined by morphological changes including cell death and by internucleosomal DNA fragmentation, occurred during culture for 1 to 2 days in a portion of the cells from three of ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(92)90393-m

    authors： Forbes IJ,Zalewski PD,Giannakis C,Cowled PA

    更新日期：1992-02-01 00:00:00

  • Differential regulation of X-chromosome-linked inhibitor of apoptosis protein (XIAP) and caspase-3 by NMDA in developing hippocampal neurons; involvement of the mitochondrial pathway in NMDA-mediated neuronal survival.

    abstract：:N-methyl-d-aspartate (NMDA) subtype of glutamate receptors plays an important role during brain development and receptor activation has been shown to promote neuronal survival. The mechanisms by which NMDA increases neuronal survival are not fully understood. Here, we show that treatment with low concentrations of NMD...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2003.12.026

    authors： Korhonen L,Näpänkangas U,Steen H,Chen Y,Martinez R,Lindholm D

    更新日期：2004-05-01 00:00:00

  • Genetic and epigenetic changes in clonal descendants of irradiated human fibroblasts.

    abstract：:To study delayed genetic and epigenetic radiation effects, which may trigger radiation-induced carcinogenesis, we have established single-cell clones from irradiated and non-irradiated primary human fibroblasts. Stable clones were endowed with the same karyotype in all analyzed metaphases after 20 population doublings...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2018.06.034

    authors： Flunkert J,Maierhofer A,Dittrich M,Müller T,Horvath S,Nanda I,Haaf T

    更新日期：2018-09-15 00:00:00

  • Characterization of human bronchial epithelial cells immortalized by the E6 and E7 genes of human papillomavirus type 16.

    abstract：:We describe a novel human bronchial epithelial cell line immortalized after transfection with the E6 and E7 genes of human papillomavirus type 16. The established HBE4-E6/E7 cell line resembles morphologically the basal cells of the normal human bronchial epithelium and is nontumorigenic. A clonal isolate proved to be...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1994.1115

    authors： Viallet J,Liu C,Emond J,Tsao MS

    更新日期：1994-05-01 00:00:00

  • Dopamine 1 receptor activation protects mouse diabetic podocytes injury via regulating the PKA/NOX-5/p38 MAPK axis.

    abstract：:Diabetic nephropathy (DN) is a major microvascular complication of diabetes that can lead to end-stage renal disease. Podocytes constitute the last barrier of glomerular filtration, whose damage are the direct cause of proteinuria. Dopamine receptors are involved in the regulation of diabetes-induced glomerular hyperf...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2020.111849

    authors： Shao X,Zhang X,Hu J,Gao T,Chen J,Xu C,Wei C

    更新日期：2020-03-15 00:00:00

  • Towards a further understanding of the growth-inhibiting action of oxygen deficiency. Evaluation of the effect of antimycin on proliferating Ehrlich ascites tumour cells.

    abstract：:The effect of 1 microM antimycin on the proliferative properties, metabolism and basic cell composition of Ehrlich ascites tumour cells cultured in the second in vitro passage was studied. Continuous drug exposure of asynchronous cells caused rapid cessation of cell growth, characterized by the cell number and DNA, RN...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(85)90162-4

    authors： Löffler M

    更新日期：1985-03-01 00:00:00

  • Demonstration of a 7-nm RNP fiber as the basic structural element in a premessenger RNP particle.

    abstract：:Balbiani ring granules in Chironomus salivary glands represent premessenger ribonucleoprotein (RNP) particles, each containing a 35- to 40-kb message for a secretory polypeptide. Their gross structure can be described as an RNP ribbon bent into a toroid. We now demonstrate that an unfolded, thin RNP fiber is observed ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(92)90437-d

    authors： Lönnroth A,Alexciev K,Mehlin H,Wurtz T,Skoglund U,Daneholt B

    更新日期：1992-04-01 00:00:00

  • Overexpression of glial cell line-derived neurotrophic factor induces genes regulating migration and differentiation of neuronal progenitor cells.

    abstract：:The glial cell line-derived neurotrophic factor (GDNF) is involved in the development and maintenance of neural tissues. Mutations in components of its signaling pathway lead to severe migration deficits of neuronal crest stem cells, tumor formation, or ablation of the urinary system. In animal models of Parkinson's d...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2004.03.037

    authors： Pahnke J,Mix E,Knoblich R,Müller J,Zschiesche M,Schubert B,Koczan D,Bauer P,Böttcher T,Thiesen HJ,Lazarov L,Wree A,Rolfs A

    更新日期：2004-07-15 00:00:00

  • Differential expression of Raf-1 protooncogene in resting and activated human leukocyte populations.

    abstract：:In this study we examined by Northern blot analysis the expression of Raf-1 protooncogene in normal human peripheral blood leukocytes. Unlike thymocytes, circulating lymphocytes did not express appreciable levels of Raf-1 mRNA. In contrast, polymorphonuclear cells (PMN) had high levels of Raf-1 transcripts. Also densi...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(91)90366-3

    authors： Colotta F,Polentarutti N,Mantovani A

    更新日期：1991-06-01 00:00:00

  • GSK-3beta inhibition by lithium confers resistance to chemotherapy-induced apoptosis through the repression of CD95 (Fas/APO-1) expression.

    abstract：:Lithium exerts neuroprotective actions that involve the inhibition of glycogen synthase kinase-3beta (GSK-3beta). Otherwise, recent studies suggest that sustained GSK-3beta inhibition is a hallmark of tumorigenesis. In this context, the present study was undertaken to examine whether lithium modulated cancer cell sens...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2004.08.001

    authors： Beurel E,Kornprobst M,Blivet-Van Eggelpoël MJ,Ruiz-Ruiz C,Cadoret A,Capeau J,Desbois-Mouthon C

    更新日期：2004-11-01 00:00:00

  • Trefoil peptides promote restitution of wounded corneal epithelial cells.

    abstract：:The ocular surface shares many characteristics with mucosal surfaces. In both, healing is regulated by peptide growth factors, cytokines, and extracellular matrix proteins. However, these factors are not sufficient to ensure most rapid healing. Trefoil peptides are abundantly expressed epithelial cell products which e...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2000.5116

    authors： Göke MN,Cook JR,Kunert KS,Fini ME,Gipson IK,Podolsky DK

    更新日期：2001-04-01 00:00:00

  • Lactate production and amino acid incorporation in interleukin 3-dependent, factor-deprived hemopoietic murine cell lines.

    abstract：:Interleukin 3 (IL-3) stimulates the growth of various types of hemopoietic progenitors. In vitro, survival of a series of murine cell lines derived from either neoplastic or nonneoplastic hemopoietic tissue shows a strict IL-3 dependence. In order to test the implication of energy metabolism in this dependence as clai...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(90)90182-a

    authors： Reisbach G,Ellwart J,Dörmer P

    更新日期：1990-10-01 00:00:00

  • Measurement of cell-monolayer adhesion in cells transfected with N-CAM cDNAs.

    abstract：:To measure the adhesion of cells expressing the neural cell adhesion molecule N-CAM, mouse Lmtk- fibroblast cells were transfected by a calcium phosphate precipitation technique with eucaryotic expression vectors encoding N-CAM polypeptides. We obtained cell lines expressing the 140-kDa transmembrane isoform of N-CAM ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1993.1041

    authors： Woo MK,Murray BA

    更新日期：1993-02-01 00:00:00

  • Induction of apoptosis in human replicative senescent fibroblasts.

    abstract：:Cellular senescence is an irreversible growth phase characteristic of normal cells. We have found that human senescent fibroblasts can be induced to undergo programmed cell death (apoptosis) by ceramide, TNF-alpha, or okadaic acid. The most profound effects were induced by TNF-alpha and okadaic acid treatment. In the ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2001.5425

    authors： DeJesus V,Rios I,Davis C,Chen Y,Calhoun D,Zakeri Z,Hubbard K

    更新日期：2002-03-10 00:00:00

  • Identification of avian sarcoplasmic reticulum Ca(2+)-ATPase (SERCA3) as a novel 1,25(OH)(2)D(3) target gene in the monocytic lineage.

    abstract：:Osteoclasts are postmitotic, multinucleated giant cells generated by the fusion of hematopoietic mononuclear precursors from the monocyte-macrophage lineage. In culture, adherent macrophages from blood-derived monocytes grow, gather, and fuse together to form multinucleated osteoclast-like cells. These events are cont...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1999.4526

    authors： Machuca I,Domenget C,Jurdic P

    更新日期：1999-08-01 00:00:00

  • An optimized method for mouse liver sinusoidal endothelial cell isolation.

    abstract：:The objective of the present study was to develop an accurate and reproducible method for liver sinusoidal endothelial cell (LSEC) isolation in mice. Non-parenchymal cells were isolated using a modified two-step collagenase digestion combined with Optiprep density gradient centrifugation. LSEC were further purified us...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2016.10.024

    authors： Meyer J,Lacotte S,Morel P,Gonelle-Gispert C,Bühler L

    更新日期：2016-12-10 00:00:00

  • Histone H3 phosphorylation in human monocytes and during HL-60 cell differentiation.

    abstract：:Phosphorylation of the nucleosome core histone H3 (H3) on Ser-10 is thought to be a prerequisite for chromatin condensation at mitosis. Although during interphase, cell differentiation, or mitogenic activation of quiescent cells, changes in chromatin structure that involve local chromatin condensation/decondensation a...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1998.4283

    authors： Juan G,Traganos F,Darzynkiewicz Z

    更新日期：1999-01-10 00:00:00

  • Reactivation of DNA replication in nuclei from terminally differentiated cells: nuclear membrane permeabilization is required for initiation in Xenopus egg extract.

    abstract：:We have used Xenopus egg extract to investigate the requirements for reactivation of DNA replication in nuclei isolated from terminally differentiated chicken erythrocytes. Previous work has shown that reactivation of erythrocyte nuclei in egg extract is accompanied by chromatin decondensation, nuclear envelope reform...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1997.3520

    authors： Leno GH,Munshi R

    更新日期：1997-05-01 00:00:00

  • High yields of specific hybridomas obtained by electrofusion of murine lymphocytes immunized in vivo or in vitro.

    abstract：:We have studied the use of electrofusion to obtain hybridomas producing antigen-specific antibodies after immunization of murine lymphocytes in vitro. Under optimal conditions fusion frequencies of the order of magnitude of 10(-3) were obtained, which is approximately 80-fold higher than the mean value obtained with f...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(89)90405-9

    authors： van Duijn G,Langedijk JP,de Boer M,Tager JM

    更新日期：1989-08-01 00:00:00

  • Induction of tyrosinase in human melanoma cells by L-tyrosine phosphate and cytochalasin D.

    abstract：:Pigmentation of RVH 421 human melanoma cells is induced when cell division is inhibited by cytochalasin D or L-tyrosine phosphate. Increased pigmentation correlates with increased tyrosinase activity when this is monitored over a time-course. Parallel measurements show that the amount of tyrosinase mRNA correlates wit...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(92)90431-7

    authors： Winder AJ,Harris H

    更新日期：1992-04-01 00:00:00