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Demonstration of a 7-nm RNP fiber as the basic structural element in a premessenger RNP particle.

Abstract：

:Balbiani ring granules in Chironomus salivary glands represent premessenger ribonucleoprotein (RNP) particles, each containing a 35- to 40-kb message for a secretory polypeptide. Their gross structure can be described as an RNP ribbon bent into a toroid. We now demonstrate that an unfolded, thin RNP fiber is observed after low salt treatment of isolated Balbiani ring granules. Moreover, the thin RNP fiber, 7 nm in diameter, can be revealed as the main structural element in Balbiani ring granules studied in situ in 3-D with electron microscope tomography. It is proposed that the thin RNP fiber consists of a premessenger RNA molecule coiled around a filamentous core of polymeric proteins, which has functional implications for processes such as assembly of RNP, intranuclear degradation of RNA, and delivery of RNA through the nuclear pores.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Lönnroth A,Alexciev K,Mehlin H,Wurtz T,Skoglund U,Daneholt B

doi

10.1016/0014-4827(92)90437-d

keywords:

subject

Has Abstract

pub_date

1992-04-01 00:00:00

pages

292-6

issue

2

eissn

0014-4827

issn

1090-2422

journal_volume

199

pub_type

杂志文章

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