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Axin is expressed in mitochondria and suppresses mitochondrial ATP synthesis in HeLa cells.

Abstract：

:Many recent studies have revealed that axin is involved in numerous cellular functions beyond the negative regulation of β-catenin-dependent Wnt signaling. Previously, an association of ectopic axin with mitochondria was observed. In an effort to investigate the relationship between axin and mitochondria, we found that axin expression suppressed cellular ATP production, which was more apparent as axin expression levels increased. Also, mitochondrial expression of axin was observed using two axin-expressing HeLa cell models: doxycycline-inducible ectopic axin expression (HeLa-axin) and axin expression enhanced by long-term treatment with XAV939 (HeLa-XAV). In biochemical analysis, axin is associated with oxidative phosphorylation (OXPHOS) complex IV and is involved in defects in the assembly of complex IV-containing supercomplexes. Functionally, axin expression reduced the activity of OXPHOS complex IV and the oxygen consumption rate (OCR), suggesting axin-mediated mitochondrial dysfunction. Subsequent studies using various inhibitors of Wnt signaling showed that the reduction in cellular ATP levels was weaker in cases of ICAT protein expression and treatment with iCRT3 or NSC668036 compared with XAV939 treatment, suggesting that XAV939 treatment affects ATP synthesis in addition to suppressing Wnt signaling activity. Axin-mediated regulation of mitochondrial function may be an additional mechanism to Wnt signaling for regulation of cell growth.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Shin JH,Kim HW,Rhyu IJ,Kee SH

doi

10.1016/j.yexcr.2015.12.003

subject

Has Abstract

pub_date

2016-01-01 00:00:00

pages

12-21

issue

1

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(15)30171-3

journal_volume

340

pub_type

杂志文章

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