当前位置： SCI文献检索 > EXPERIMENTAL CELL RESEARCH期刊下所有文献 > Skeletal myocyte hypertrophy requires mTOR kinase activity and S6K1.

Skeletal myocyte hypertrophy requires mTOR kinase activity and S6K1.

Abstract：

:The protein kinase mammalian target of rapamycin (mTOR) is a central regulator of cell proliferation and growth, with the ribosomal subunit S6 kinase 1 (S6K1) as one of the key downstream signaling effectors. A critical role of mTOR signaling in skeletal muscle differentiation has been identified recently, and an unusual regulatory mechanism independent of mTOR kinase activity and S6K1 is revealed. An mTOR pathway has also been reported to regulate skeletal muscle hypertrophy, but the regulatory mechanism is not completely understood. Here, we report the investigation of mTOR's function in insulin growth factor I (IGF-I)-induced C2C12 myotube hypertrophy. Added at a later stage when rapamycin no longer had any effect on normal myocyte differentiation, rapamycin completely blocked myocyte hypertrophy as measured by myotube diameter. Importantly, a concerted increase of average myonuclei per myotube was observed in IGF-I-stimulated myotubes, which was also inhibited by rapamycin added at a time when it no longer affected normal differentiation. The mTOR protein level, its catalytic activity, its phosphorylation on Ser2448, and the activity of S6K1 were all found increased in IGF-I-stimulated myotubes compared to unstimulated myotubes. Using C2C12 cells stably expressing rapamycin-resistant forms of mTOR and S6K1, we provide genetic evidence for the requirement of mTOR and its downstream effector S6K1 in the regulation of myotube hypertrophy. Our results suggest distinct mTOR signaling mechanisms in different stages of skeletal muscle development: While mTOR regulates the initial myoblast differentiation in a kinase-independent and S6K1-independent manner, the hypertrophic function of mTOR requires its kinase activity and employs S6K1 as a downstream effector.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Park IH,Erbay E,Nuzzi P,Chen J

doi

10.1016/j.yexcr.2005.05.017

keywords:

subject

Has Abstract

pub_date

2005-09-10 00:00:00

pages

211-9

issue

1

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(05)00247-8

journal_volume

309

pub_type

杂志文章

相关文献

EXPERIMENTAL CELL RESEARCH文献大全
  • Oocyte-specific deletion of Gsα induces oxidative stress and deteriorates oocyte quality in mice.

    abstract：:The stimulatory heterotrimeric Gs protein alpha subunit (Gsα) is a ubiquitous guanine nucleotide-binding protein that regulates the intracellular cAMP signaling pathway and consequently participates in a wide range of biological events. In the reproductive system, despite Gsα being associated with oocyte meiotic arres...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2018.07.023

    authors： Xie Y,Wu B,Jin Y,Zhang A,Sun X,Zhang X,Gao X,Dong R,Li H,Gao J

    更新日期：2018-09-15 00:00:00

  • Age-dependent migratory behavior of human endothelial cells revealed by substrate microtopography.

    abstract：:Cell migration is part of many important in vivo biological processes and is influenced by chemical and physical factors such as substrate topography. Although the migratory behavior of different cell types on structured substrates has already been investigated, up to date it is largely unknown if specimen's age affec...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2018.10.008

    authors： Sales A,Picart C,Kemkemer R

    更新日期：2019-01-01 00:00:00

  • Expression of spasmolysin (FIM-A.1): an integumentary mucin from Xenopus laevis.

    abstract：:In the past, a unique type of precursor for a secretory protein was discovered. It contains a central repetitive domain rich in threonine residues and terminal cysteine-rich domains. Due to striking homologies of these terminal domains with pancreatic spasmolytic polypeptide, originally the name "prepro-spasmolysin" w...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(90)90230-8

    authors： Hauser F,Gertzen EM,Hoffmann W

    更新日期：1990-08-01 00:00:00

  • Hsp90 inhibitor 17-AAG sensitizes Bcl-2 inhibitor (-)-gossypol by suppressing ERK-mediated protective autophagy and Mcl-1 accumulation in hepatocellular carcinoma cells.

    abstract：:Natural BH3-memitic (-)-gossypol shows promising antitumor efficacy in several kinds of cancer. However, our previous studies have demonstrated that protective autophagy decreases the drug sensitivities of Bcl-2 inhibitors in hepatocellular carcinoma (HCC) cells. In the present study, we are the first to report that H...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2014.08.039

    authors： Wang B,Chen L,Ni Z,Dai X,Qin L,Wu Y,Li X,Xu L,Lian J,He F

    更新日期：2014-11-01 00:00:00

  • Spontaneous apoptosis of thymocytes is uncoupled with progression through the cell cycle.

    abstract：:Most developing lymphocytes spontaneously die in the thymus during positive and negative selection of the T cell repertoire. By evaluating the expression of the proliferation antigens Ki-67 and PCNA, we demonstrated here that more than 95% of thymocytes are potentially proliferating. The coincidence within the same ce...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1996.0382

    authors： Pellicciari C,Bottone MG,Schaack V,Barni S,Manfredi AA

    更新日期：1996-12-15 00:00:00

  • Effect of shear stress on cytosolic Ca2+ of calf pulmonary artery endothelial cells.

    abstract：:The purpose of the present study was to determine if hemodynamic shear stress increases free cytosolic Ca2+ concentration ([Ca2+]i) of cultured pulmonary artery endothelial cells exposed to steady laminar fluid flow in a parallel plate chamber. Average [Ca2+]i was estimated by measuring cell-associated fura-2 fluoresc...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(92)90145-x

    authors： Schilling WP,Mo M,Eskin SG

    更新日期：1992-01-01 00:00:00

  • Nuclear domains in skeletal myotubes: the localization of transferrin receptor mRNA is independent of its half-life and restricted by binding to ribosomes.

    abstract：:The retention of mRNAs near the nuclei that synthesize them may be an important feature of the organization of multinucleated skeletal myotubes. Here, we assess the possible role of two factors in this localization. First, we examine the role of mRNA half-life, by studying the distribution of the mRNA for the transfer...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1997.3753

    authors： Ralston E,McLaren RS,Horowitz JA

    更新日期：1997-11-01 00:00:00

  • Poly(ADP ribose) polymerase-defective mutant cell clone of mouse L1210 cells.

    abstract：:By a sequential mutation and selection utilizing N-methyl-N'-nitro-N-nitrosoguanidine as a mutagen, we succeeded in separating a poly(ADP ribose) polymerase-defective mutant clone (Cl-3527) from a mouse L1210 cell clone (Cl-3). The enzyme activity per cell in Cl-3527 cells was only 8% of that in wild type L1210 (CCL 2...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/s0014-4827(05)80080-1

    authors： Yoshihara K,Itaya A,Hironaka T,Sakuramoto S,Tanaka Y,Tsuyuki M,Inada Y,Kamiya T,Ohnishi K,Honma M

    更新日期：1992-05-01 00:00:00

  • Peroxiredoxin V is essential for protection against apoptosis in human lung carcinoma cells.

    abstract：:Sensitivity of tumor cells to treatment with anticancer drugs depends on expression and function of antiapoptotic and antioxidant proteins. The goal of our study was to determine the functional role of the novel antioxidant protein Peroxiredoxin V (PrxV), in protection of human lung carcinoma cell lines against apopto...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2006.05.006

    authors： Kropotov A,Gogvadze V,Shupliakov O,Tomilin N,Serikov VB,Tomilin NV,Zhivotovsky B

    更新日期：2006-09-10 00:00:00

  • In vitro chondrogenesis of bone marrow-derived mesenchymal progenitor cells.

    abstract：:A culture system that facilitates the chondrogenic differentiation of rabbit bone marrow-derived mesenchymal progenitor cells has been developed. Cells obtained in bone marrow aspirates were first isolated by monolayer culture and then transferred into tubes and allowed to form three-dimensional aggregates in a chemic...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1997.3858

    authors： Johnstone B,Hering TM,Caplan AI,Goldberg VM,Yoo JU

    更新日期：1998-01-10 00:00:00

  • SIX4 activates Akt and promotes tumor angiogenesis.

    abstract：:Angiogenesis plays important roles in solid tumors progression. Growth factors such as vascular endothelial growth factors (VEGFs) can induce angiogenesis and hypoxia promotes the expression of VEGFs through activating hypoxia-inducible factor 1 (HIF-1α). However, the regulation of HIF-1α still not been fully understo...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2019.111495

    authors： Sun X,Hu F,Hou Z,Chen Q,Lan J,Luo X,Wang G,Hu J,Cao Z

    更新日期：2019-10-01 00:00:00

  • Inhibition of tumor growth by a truncated and soluble form of melanotransferrin.

    abstract：:Melanotransferrin is a glycoprotein expressed at the cell membrane and secreted in the extracellular environment. Recombinant truncated form of membrane-bound melanotransferrin (sMTf) was reported to exert in vitro anti-angiogenic properties. Here we show that sMTf treatment leads to a 50% inhibition of neovasculariza...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2007.04.013

    authors： Rolland Y,Demeule M,Michaud-Levesque J,Béliveau R

    更新日期：2007-08-01 00:00:00

  • Syndecan-1 ectodomain regulates matrix-dependent signaling in human breast carcinoma cells.

    abstract：:Syndecan-1 was overexpressed in T47D, MCF-7, or Hs578t human breast carcinoma cell lines, mimicking overexpression observed in carcinomas in vivo. Overexpression of syndecan-1, or its ectodomain alone fused to a glycosylphosphatidylinositol anchor (GPI-mS1ED), promotes cell rounding in 2D culture. Deletions within the...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2004.07.001

    authors： Burbach BJ,Ji Y,Rapraeger AC

    更新日期：2004-10-15 00:00:00

  • Ruta graveolens water extract inhibits cell-cell network formation in human umbilical endothelial cells via MEK-ERK1/2 pathway.

    abstract：:Angiogenesis is a process encompassing several steps such as endothelial cells proliferation, differentiation and migration to form a vascular network, involving different signal transduction pathways. Among these, ERK1/2 signaling mediates VEGF-dependent signaling pathway. Here we report that the water extract of Rut...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2018.01.025

    authors： Gentile MT,Russo R,Pastorino O,Cioffi S,Barbieri F,Illingworth EA,Grieco M,Chambery A,Colucci-D'Amato L

    更新日期：2018-03-01 00:00:00

  • p53-independent upregulation of KILLER/DR5 TRAIL receptor expression by glucocorticoids and interferon-gamma.

    abstract：:KILLER/DR5 is a death-domain-containing proapoptotic receptor that binds to the cytotoxic ligand TRAIL. It was originally reported that induction of KILLER/DR5 mRNA following DNA damage was p53-dependent, but some drugs that induce apoptosis can upregulate KILLER/DR5 mRNA expression in cell lines with mutated p53. We ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2000.5073

    authors： Meng RD,El-Deiry WS

    更新日期：2001-01-15 00:00:00

  • RCAN1.4 acts as a suppressor of cancer progression and sunitinib resistance in clear cell renal cell carcinoma.

    abstract：:Clear cell renal cell carcinoma (ccRCC) is one of the most common malignant tumors in the urinary system, and its incidence continues to increase. Regulator of calcineurin 1 (RCAN1), one of the genes on chromosome 21, is a crucial mediator of tumor inhibition. RCAN1.4 is best characterized as an endogenous inhibitor o...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2018.09.017

    authors： Song Z,Cao Q,Ruan H,Yang H,Wang K,Bao L,Cheng G,Xu T,Xiao H,Wang C,Liu D,Chen K,Zhang X

    更新日期：2018-11-15 00:00:00

  • Mechanical regulation of myofibroblast phenoconversion and collagen contraction.

    abstract：:Activated fibroblasts promote physiological wound repair following tissue injury. However, dysregulation of fibroblast activation contributes to the development of fibrosis by enhanced production and contraction of collagen-rich extracellular matrix. At the peak of their activities, fibroblasts undergo phenotypic conv...

    journal_title：Experimental cell research

    pub_type： 杂志文章,评审

    doi：10.1016/j.yexcr.2019.03.027

    authors： Hinz B,McCulloch CA,Coelho NM

    更新日期：2019-06-01 00:00:00

  • Inorganic phosphate regulates multiple genes during osteoblast differentiation, including Nrf2.

    abstract：:The process of osteoblast differentiation and matrix mineralization requires a rise in alkaline phosphatase enzymatic activity resulting in the generation of free phosphate. The ability of inorganic phosphate to regulate gene transcription and cellular function represents a potentially novel extracellular signaling me...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/s0014-4827(03)00213-1

    authors： Beck GR Jr,Moran E,Knecht N

    更新日期：2003-08-15 00:00:00

  • Activation of a cAMP pathway and induction of melanogenesis correlate with association of p16(INK4) and p27(KIP1) to CDKs, loss of E2F-binding activity, and premature senescence of human melanocytes.

    abstract：:There is strong evidence that the senescent phenotype, whether induced by telomere shortening, oxidative damage, or oncogenic stimuli, is an important tumor suppressive mechanism. The melanocyte is a cell of neural crest origin that produces the pigment melanin and can develop into malignant melanomas. To understand h...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1999.4688

    authors： Haddad MM,Xu W,Schwahn DJ,Liao F,Medrano EE

    更新日期：1999-12-15 00:00:00

  • Down-regulation of RBP-J mediated by microRNA-133a suppresses dendritic cells and functions as a potential tumor suppressor in osteosarcoma.

    abstract：BACKGROUND AND OBJECTIVE:In recent years, immunotherapy for the treatment of tumors have been established. Dendritic cells (DCs) are extremely efficient and professional antigen presenting cells (APCs), which are an important target for immune therapeutic interventions in cancer. In present study, we investigated wheth...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2016.10.019

    authors： Gao X,Han D,Fan W

    更新日期：2016-12-10 00:00:00

  • eIF4E-Overexpression imparts perillyl alcohol and rapamycin-mediated regulation of telomerase reverse transcriptase.

    abstract：:Translation is mediated partly by regulation of free eukaryotic initiation factor 4E (eIF4E) levels through PI3K-Akt-mTOR signaling. Cancer cells treated with the plant-derived perillyl alcohol (POH) or the mechanistic target of rapamycin (mTOR) inhibitor rapamycin dephosphorylate eIF4E-binding protein (4E-BP1) and at...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2013.05.029

    authors： Sundin T,Peffley D,Hentosh P

    更新日期：2013-08-01 00:00:00

  • Recruitment of phosphorylated small heat shock protein Hsp27 to nuclear speckles without stress.

    abstract：:During stress, the mammalian small heat shock protein Hsp27 enters cell nuclei. The present study examines the requirements for entry of Hsp27 into nuclei of normal rat kidney (NRK) renal epithelial cells, and for its interactions with specific nuclear structures. We find that phosphorylation of Hsp27 is necessary for...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2006.10.004

    authors： Bryantsev AL,Chechenova MB,Shelden EA

    更新日期：2007-01-01 00:00:00

  • Heterotrimeric G-proteins are implicated in the regulation of apoptosis in pancreatic beta-cells.

    abstract：:Recent studies have provided evidence that apoptosis of pancreatic beta-cells is important in the early etiology of both type I and type II diabetes mellitus. The mechanisms responsible for induction of apoptosis are unknown, but we present evidence that the signal transduction pathway controlling the process in pancr...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1996.0344

    authors： Loweth AC,Williams GT,Scarpello JH,Morgan NG

    更新日期：1996-11-25 00:00:00

  • Induction of intercellular communications in epithelial cell cultures.

    abstract：:A popular criterion of cell-cell communication in tissue cultures is dye coupling: the ability of the injected fluorescent dye of low molecular weight to be transferred from one cell to another. We report about a new factor which induces cell-to-cell dye coupling in previously uncoupled epithelial sheets. Paradoxicall...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(88)90204-2

    authors： Sharovskaja YuYu,Chailakhjan LM,Margolis LB

    更新日期：1988-04-01 00:00:00

  • PKC 412 sensitizes U1810 non-small cell lung cancer cells to DNA damage.

    abstract：:Non-small cell lung carcinoma (NSCLC) is characterized by resistance to drug-induced apoptosis, which might explain the survival of lung cancer cells following treatment. Recently we have shown that the broad-range kinase inhibitor staurosporine (STS) reactivates the apoptotic machinery in U1810 NSCLC cells [Joseph et...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2004.12.017

    authors： Hemström TH,Joseph B,Schulte G,Lewensohn R,Zhivotovsky B

    更新日期：2005-04-15 00:00:00

  • Sirtuin 5 overexpression attenuates glucolipotoxicity-induced pancreatic β cells apoptosis and dysfunction.

    abstract：:Recently, SIRT5 was reported to be a predominant desuccinylase and demalonylase in mitochondria. Ablation of SIRT5 enhances the systemic succinylation and malonylation of mitochondrial proteins, including various metabolic enzymes; however, its function in pancreatic β cells has not yet been clarified. In this study, ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2018.08.011

    authors： Wang Y,Liu Q,Huan Y,Li R,Li C,Sun S,Guo N,Yang M,Liu S,Shen Z

    更新日期：2018-10-01 00:00:00

  • Selective photothermal efficiency of citrate capped gold nanoparticles for destruction of cancer cells.

    abstract：:Gold nanoparticles are recently having much attention because of their increased applications in biomedical fields. In this paper, we demonstrated the photothermal efficacy of citrate capped gold nanoparticles (AuNPs) for the destruction of A431 cancer cells. Citrate capped AuNPs were synthesized successfully and char...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2011.04.010

    authors： Raji V,Kumar J,Rejiya CS,Vibin M,Shenoi VN,Abraham A

    更新日期：2011-08-15 00:00:00

  • In vitro replication and differentiation of normal human oral keratinocytes.

    abstract：:The replication kinetics and cytological changes of normal human oral keratinocytes (NHOK) isolated from the basal surface of oral epithelial sheet and cultured as dispersed cells in low (0.15 mM) Ca(2+) medium without serum were analyzed. Replicating NHOK were quantitated by cell count and identified by [(3)H]thymidi...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2000.4943

    authors： Kang MK,Bibb C,Baluda MA,Rey O,Park NH

    更新日期：2000-08-01 00:00:00

  • The metastasis suppressor NME1 inhibits melanoma cell motility via direct transcriptional induction of the integrin beta-3 gene.

    abstract：:Expression of the metastasis suppressor NME1 in melanoma is associated with reduced cellular motility, invasion, and metastasis, but mechanisms underlying these activities are not completely understood. Herein we report a novel mechanism through which NME1 drives formation of large, stable focal adhesions (FAs) in mel...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2018.11.010

    authors： Leonard MK,Novak M,Snyder D,Snow G,Pamidimukkala N,McCorkle JR,Yang XH,Kaetzel DM

    更新日期：2019-01-01 00:00:00

  • The over-expression of HAS2, Hyal-2 and CD44 is implicated in the invasiveness of breast cancer.

    abstract：:Within tumors there appears to be an intricate balance between hyaluronan (HA) synthesis and degradation where the invading edges display increased HA metabolism. The metabolism of HA has not been characterized in breast cancer cell lines; therefore, this study quantitatively identifies and characterizes the enzymes r...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2005.07.026

    authors： Udabage L,Brownlee GR,Nilsson SK,Brown TJ

    更新日期：2005-10-15 00:00:00