当前位置： SCI文献检索 > EXPERIMENTAL CELL RESEARCH期刊下所有文献 > Peroxiredoxin V is essential for protection against apoptosis in human lung carcinoma cells.

Peroxiredoxin V is essential for protection against apoptosis in human lung carcinoma cells.

Abstract：

:Sensitivity of tumor cells to treatment with anticancer drugs depends on expression and function of antiapoptotic and antioxidant proteins. The goal of our study was to determine the functional role of the novel antioxidant protein Peroxiredoxin V (PrxV), in protection of human lung carcinoma cell lines against apoptosis. Analysis of expression of PrxV in multiple lung carcinoma cell lines revealed a positive correlation between the expression of PrxV and radioresistance in vitro. Clones of the lung carcinoma cells U1810 with down-regulated expression of PrxV, or with its impaired enzymatic function (expression of redox-negative PrxV), demonstrated increased sensitivity to treatment with anticancer drugs etoposide and adriamycin. Pre-treatment of these clones with antioxidant N-acetyl-cysteine did not change their sensitivity to adriamycin, suggesting the involvement of a non-redox activity of PrxV. Expression of the redox-negative PrxV mainly affected the mitochondrial pathway of apoptosis, as assessed by cytochrome c release assay. Impairment of the PrxV enzymatic function also affected transmembrane potential and calcium loading capacity of mitochondria, as well as mitochondrial morphology. Altogether, these findings suggest that PrxV is a multifunctional protein, which is essential for protection against apoptosis induced by anticancer drugs.

journal_name

Exp Cell Res

journal_title

Experimental cell research

authors

Kropotov A,Gogvadze V,Shupliakov O,Tomilin N,Serikov VB,Tomilin NV,Zhivotovsky B

doi

10.1016/j.yexcr.2006.05.006

subject

Has Abstract

pub_date

2006-09-10 00:00:00

pages

2806-15

issue

15

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(06)00187-X

journal_volume

312

pub_type

杂志文章

相关文献

EXPERIMENTAL CELL RESEARCH文献大全
  • Establishment of a germ-line competent C57BL/6 embryonic stem cell line.

    abstract：:Embryonic stem (ES) cell lines have been derived from blastocysts of the inbred mouse strain C57BL/6. The highest frequencies of ES cell colonies were observed when blastocysts were explanted directly onto growth-arrested feeder layers of 5637 human bladder carcinoma cells in the presence of conditioned medium. One of...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(91)90430-3

    authors： Ledermann B,Bürki K

    更新日期：1991-12-01 00:00:00

  • Nonmuscle and smooth muscle myosin isoforms in bovine endothelial cells.

    abstract：:A panel of monoclonal antibodies, specific for human platelet (NM-A9, NM-F6, and NM-G2) and for bovine smooth muscle (SM-E7) myosin heavy chains (MHC), were used to study the composition and the distribution of myosin isoforms in bovine endothelial cells (EC), in vivo and in vitro. Using indirect and double immunofluo...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(90)90136-x

    authors： Borrione AC,Zanellato AM,Giuriato L,Scannapieco G,Pauletto P,Sartore S

    更新日期：1990-09-01 00:00:00

  • Inhibitors of phosphoinositide 3-kinase cause defects in the postendocytic sorting of beta2-adrenergic receptors.

    abstract：:Phosphatidylinositol 3-kinase inhibitors have been shown to affect endocytosis or subsequent intracellular sorting in various receptor systems. Agonist-activated beta(2)-adrenergic receptors undergo desensitization by mechanisms that include the phosphorylation, endocytosis and degradation of receptors. Following endo...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2007.04.034

    authors： Awwad HO,Iyer V,Rosenfeld JL,Millman EE,Foster E,Moore RH,Knoll BJ

    更新日期：2007-07-15 00:00:00

  • A SUV39H1-low chromatin state characterises and promotes migratory properties of cervical cancer cells.

    abstract：:Metastatic progression is a major cause of mortality in cervical cancers, but factors regulating migratory and pre-metastatic cell populations remain poorly understood. Here, we sought to assess whether a SUV39H1-low chromatin state promotes migratory cell populations in cervical cancers, using meta-analysis of data f...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2019.02.010

    authors： Rodrigues C,Pattabiraman C,Vijaykumar A,Arora R,Narayana SM,Kumar RV,Notani D,Varga-Weisz P,Krishna S

    更新日期：2019-05-15 00:00:00

  • Morphology of endoplasmic reticulum and Golgi elements following microinjection of rat liver microsomes into Xenopus laevis oocyte cytoplasm.

    abstract：:Fragments of rough endoplasmic reticulum and Golgi complex purified from rat liver homogenates were injected into Xenopus oocytes and the sites of microinjection analysed by electron microscopy at different times post-injection. The in vivo incubated fragments were located by their proximity to a microinjection vacuol...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(86)90495-7

    authors： Paiement J

    更新日期：1986-10-01 00:00:00

  • Melanoma cells produce multiple laminin isoforms and strongly migrate on α5 laminin(s) via several integrin receptors.

    abstract：:Melanoma cells express and interact with laminins (LMs) and other basement membrane components during invasion and metastasis. In the present study we have investigated the production and migration-promoting activity of laminin isoforms in melanoma. Immunohistochemistry of melanoma specimens and immunoprecipitation/we...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2010.12.019

    authors： Oikawa Y,Hansson J,Sasaki T,Rousselle P,Domogatskaya A,Rodin S,Tryggvason K,Patarroyo M

    更新日期：2011-05-01 00:00:00

  • Induction of apoptosis by cancer chemotherapy.

    abstract：:Studies performed over the past five years have demonstrated that there are two major cell-intrinsic pathways for inducing apoptosis, one that begins with ligation of cell surface death receptors and another that involves mitochondrial release of cytochrome c. Several reports have suggested that anticancer drugs kill ...

    journal_title：Experimental cell research

    pub_type： 杂志文章,评审

    doi：10.1006/excr.2000.4838

    authors： Kaufmann SH,Earnshaw WC

    更新日期：2000-04-10 00:00:00

  • Conditional inactivation of Brca1 in the mouse ovarian surface epithelium results in an increase in preneoplastic changes.

    abstract：:Epithelial ovarian cancer (EOC) is thought to arise from the ovarian surface epithelium (OSE); however, the molecular events underlying this transformation are poorly understood. Germline mutations in the BRCA1 tumor suppressor gene result in a significantly increased risk of developing EOC and a large proportion of s...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2006.09.026

    authors： Clark-Knowles KV,Garson K,Jonkers J,Vanderhyden BC

    更新日期：2007-01-01 00:00:00

  • Transforming NRK cells with avian sarcoma virus reduces the extracellular Ca2+ requirement without affecting the calcicalmodulin requirement for the G1/S transition.

    abstract：:NRK rat cells infected with a transformation-defective, temperature-sensitive (ts) mutant of the avian sarcoma virus could not proliferate in Ca2+-deficient medium at a nonpermissive temperature (40 degrees C) that inactivated the viral pp60v-src-transforming product and rendered the cells phenotypically untransformed...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(85)90140-5

    authors： Durkin JP,Whitfield JF

    更新日期：1985-04-01 00:00:00

  • Hair follicle-specific keratins and their diseases.

    abstract：:The human keratin family comprises 54 members, 28 type I and 26 type II. Out of the 28 type I keratins, 17 are epithelial and 11 are hair keratins. Similarly, the 26 type II members comprise 20 epithelial and 6 hair keratins. As, however, 9 out of the 37 epithelial keratins are specifically expressed in the hair folli...

    journal_title：Experimental cell research

    pub_type： 杂志文章,评审

    doi：10.1016/j.yexcr.2007.02.032

    authors： Schweizer J,Langbein L,Rogers MA,Winter H

    更新日期：2007-06-10 00:00:00

  • MIP-1alpha induces activation of phosphatidylinositol-3 kinase that associates with Pyk-2 and is necessary for B-cell migration.

    abstract：:The chemokine macrophage inflammatory protein-1 alpha [MIP-1alpha] causes migration of B cells and also induces changes in antibody secretion. However, the signal transduction pathways leading to these phenotypic changes remain undefined. We have identified a signal transduction pathway initiated by MIP-1alpha in B ce...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2001.5272

    authors： Rumsey LM,Teague RM,Benedict SH,Chan MA

    更新日期：2001-08-01 00:00:00

  • Scavenger receptors mediate adhesion of activated B lymphocytes.

    abstract：:The scavenger receptor (SR-A) is considered to play a role in host defense by scavenging endotoxins, oxidized proteins, and denatured or otherwise modified self components, which are routed toward degradation in macrophages. Recent data suggest that SR-A also functions as an adhesion molecule. Our previous finding of ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1997.3876

    authors： Yokota T,Ehlin-Henriksson B,Hansson GK

    更新日期：1998-02-25 00:00:00

  • Diadenosine tetraphosphate (Ap4A) is compartmentalized in nuclei of mammalian cells.

    abstract：:The intracellular compartmentation of Ap4A in various growth and cell-cycle stages in mammalian cells was studied by applying a non-aqueous extraction procedure for cell nuclei. In both slowly and in exponentially growing Ehrlich ascites tumour cells from random cultures, more than 75% of the whole cellular Ap4A conte...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(86)90607-5

    authors： Weinmann-Dorsch C,Grummt F

    更新日期：1986-08-01 00:00:00

  • ES cell cycle rates affect gene targeting frequencies.

    abstract：:We have investigated the gene targeting frequency at the hprt locus in a range of embryonic stem cell lines selected for variations in cell cycle parameters. Our results show that targeting frequency varies with cell line by as much as 12-fold between nonisogenic lines and 3-fold between isogenic lines and that a noni...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1996.3470

    authors： Udy GB,Parkes BD,Wells DN

    更新日期：1997-03-15 00:00:00

  • Species-divergent regulation of human and mouse osteocalcin genes by calciotropic hormones.

    abstract：:Although osteocalcin is the most abundant noncollagenous protein in bone, its role remains undefined. Recent studies have reported diametrically opposing responses in the vitamin D regulation of the mouse vs the human and rat osteocalcin genes. The aim of this study was to increase the understanding of these differenc...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2000.4912

    authors： Thomas GP,Bourne A,Eisman JA,Gardiner EM

    更新日期：2000-08-01 00:00:00

  • Characterization of STIP, a multi-domain nuclear protein, highly conserved in metazoans, and essential for embryogenesis in Caenorhabditis elegans.

    abstract：:We report here the identification and characterization of STIP, a multi-domain nuclear protein that contains a G-patch, a coiled-coil, and several short tryptophan-tryptophan repeats highly conserved in metazoan species. To analyze their functional role in vivo, we cloned nematode stip-1 genes and determined the spati...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2007.01.003

    authors： Ji Q,Huang CH,Peng J,Hashmi S,Ye T,Chen Y

    更新日期：2007-04-15 00:00:00

  • A cytosolic activator of DNA replication is tyrosine phosphorylated in its active form.

    abstract：:Cytosolic extracts from actively dividing lymphoid cells have been shown to induce DNA synthesis in isolated, quiescent nuclei. An initiating factor in such extracts (activator of DNA replication; ADR) is a > 90-kDa aprotinin-binding protein whose activity is inhibitable not only by aprotinin, but also by several othe...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1993.1090

    authors： Fresa KL,Autieri MV,Coffman FD,Georgoff I,Cohen S

    更新日期：1993-04-01 00:00:00

  • Modification of integrin-mediated cell attachment to substrata by serine proteinases in the presence and absence of divalent cations.

    abstract：:The sensitivity to serine proteinases of cellular proteins involved in cell-matrix adhesion was investigated using C32 melanoma cells. Cells dissociated from monolayers by the metal chelator ethylenediaminetetraacetic acid were incubated with proteolytic enzymes, and then attachment was quantified by standard cell adh...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1993.1226

    authors： Fujii K,Lazarus GS,Schechter NM

    更新日期：1993-09-01 00:00:00

  • Thrombin peptide, TP508, induces differential gene expression in fibroblasts through a nonproteolytic activation pathway.

    abstract：:Prior studies have shown that synthetic peptides representing the domain of thrombin responsible for high-affinity binding to fibroblasts stimulate chemotactic and cell proliferative signals through a nonproteolytic mechanism. One of these peptides, TP508, has recently been shown to be chemotactic for neutrophils, to ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1998.4372

    authors： Sower LE,Payne DA,Meyers R,Carney DH

    更新日期：1999-03-15 00:00:00

  • Alpha-actinin arcs in megakaryocyte spreading.

    abstract：:Cultured megakaryocytes, isolated from guinea pig bone marrow, were treated with buffer or adenosine diphosphate (ADP) (10 microM) on plain or coated glass surfaces. Control cells were rounded and non-adherent. The nucleotide induced the cells to spread to several times the initial diameter, and to become flattened an...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/0014-4827(84)90649-9

    authors： Leven RM,Nachmias VT

    更新日期：1984-06-01 00:00:00

  • Long non-coding RNA HNF1A-AS1 upregulates OTX1 to enhance angiogenesis in colon cancer via the binding of transcription factor PBX3.

    abstract：:Colon cancer shows characteristics of metastasis, which is associated with angiogenesis. Increasing evidence highlights long non-coding RNAs (lncRNAs) as important participants in angiogenesis of cancers, including colon cancer. Hence, this study investigated the role of HNF1A-AS1 in angiogenesis of colon cancer. RT-q...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2020.112025

    authors： Wu J,Meng X,Jia Y,Chai J,Wang J,Xue X,Dang T

    更新日期：2020-08-15 00:00:00

  • Abnormal sperm parameters in humans are indicative of an abortive apoptotic mechanism linked to the Fas-mediated pathway.

    abstract：:The life cycle of many cell types can hinge on the presence of death factors that can control programmed cell death. The Fas-mediated apoptotic pathway has been implicated in controlling apoptosis during spermatogenesis in a number of mammalian species. In the human, the presence of nuclear DNA damage in ejaculated sp...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1999.4586

    authors： Sakkas D,Mariethoz E,St John JC

    更新日期：1999-09-15 00:00:00

  • Tetracyclines cause cell stress-dependent ATF4 activation and mTOR inhibition.

    abstract：:Tetracyclines have long been used as valuable broad-spectrum antibiotics. The high antibacterial activity of tetracyclines, combined with their good tolerability, has led to their widespread use in treating various infectious diseases. However, similar to other antibiotics, tetracyclines are also known for their adver...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2013.11.012

    authors： Brüning A,Brem GJ,Vogel M,Mylonas I

    更新日期：2014-01-15 00:00:00

  • An enhancer required for transcription of the Col6a1 gene in muscle connective tissue is induced by signals released from muscle cells.

    abstract：:Collagen VI is a survival factor for skeletal muscle produced by endomysial cells and localized in connective tissue around muscle fibers. Mutations of its genes (COL6A1, COL6A2 and COL6A3) cause two muscular disorders, Bethlem myopathy and Ullrich disease. Expression of Collagen VI is highly dynamic during developmen...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2008.08.006

    authors： Braghetta P,Ferrari A,Fabbro C,Bizzotto D,Volpin D,Bonaldo P,Bressan GM

    更新日期：2008-11-15 00:00:00

  • The nuclear factor kappa B (NF-κB) activation is required for phagocytosis of staphylococcus aureus by RAW 264.7 cells.

    abstract：:Nuclear factor kappa B (NF-κB) is a ubiquitous transcription factor which controls the expression of various genes involved in immune responses. However, it is not clear whether NF-κB activation is critical for phagocytosis when Staphylococcus aureus is the pathogen. Using oligonucleotide microarrays, we investigated ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2014.04.018

    authors： Zhu F,Yue W,Wang Y

    更新日期：2014-10-01 00:00:00

  • Anchorage-independent culture of bovine granulosa cells: the effects of basic fibroblast growth factor and dibutyryl cAMP on cell division and differentiation.

    abstract：:During ovarian folliculogenesis granulosa cells divide while in contact with stromal cells and other granulosa cells. Following ovulation, however, they cease dividing and differentiate into large luteal cells. When cultured in monolayer, granulosa cells spontaneously differentiate into luteal cells, thus confounding ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1994.1084

    authors： Lavranos TC,Rodgers HF,Bertoncello I,Rodgers RJ

    更新日期：1994-04-01 00:00:00

  • Measurement of cell-monolayer adhesion in cells transfected with N-CAM cDNAs.

    abstract：:To measure the adhesion of cells expressing the neural cell adhesion molecule N-CAM, mouse Lmtk- fibroblast cells were transfected by a calcium phosphate precipitation technique with eucaryotic expression vectors encoding N-CAM polypeptides. We obtained cell lines expressing the 140-kDa transmembrane isoform of N-CAM ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.1993.1041

    authors： Woo MK,Murray BA

    更新日期：1993-02-01 00:00:00

  • HaCaT keratinocyte migration is dependent on epidermal growth factor receptor signaling and glycogen synthase kinase-3alpha.

    abstract：:After epithelial disruption by tissue injury, keratinocytes migrate from the wound edge into a provisional matrix. This process is stimulated by growth factors that signal through epidermal growth factor (EGF) receptor, including EGF, heparin-binding EGF-like growth factor (HB-EGF) and transforming growth factor-alpha...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/j.yexcr.2006.05.009

    authors： Koivisto L,Jiang G,Häkkinen L,Chan B,Larjava H

    更新日期：2006-09-10 00:00:00

  • Human telomerase can immortalize Indian muntjac cells.

    abstract：:The mechanisms of replicative senescence by telomere shortening are not fully understood. The Indian muntjac has the fewest chromosomes of all mammals, greatly simplifying the analysis of each telomere over time. In this study, telomere shortening was observed throughout the life span of cultured normal muntjac cells ...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1006/excr.2002.5645

    authors： Zou Y,Yi X,Wright WE,Shay JW

    更新日期：2002-11-15 00:00:00

  • 5-azacytidine reactivates the erythroid differentiation potential of the myeloid-restricted murine cell line 32D Ro.

    abstract：:32D cells grown for 1 year in interleukin-3 (IL-3) and granulocyte colony-stimulating factor (G-CSF) generated the 32D Ro cell line which retained the parental mast cell phenotype but lost ability to generate erythroid cells in response to erythropoietin (EPO). In order to clarify the mechanisms underlying such restri...

    journal_title：Experimental cell research

    pub_type： 杂志文章

    doi：10.1016/s0014-4827(03)00032-6

    authors： Baiocchi M,Di Rico C,Di Pietro R,Di Baldassarre A,Migliaccio AR

    更新日期：2003-05-01 00:00:00