Induction of intercellular communications in epithelial cell cultures.

Abstract:

:A popular criterion of cell-cell communication in tissue cultures is dye coupling: the ability of the injected fluorescent dye of low molecular weight to be transferred from one cell to another. We report about a new factor which induces cell-to-cell dye coupling in previously uncoupled epithelial sheets. Paradoxically it is the standard fluorescent microscopy itself (that is, blue light of 320- to 480-nm wavelength) which induces rapid morphological alterations of cell culture followed by the transfer of fluorescent dye from one cell to another. Thus monitoring cell-cell dye coupling by fluorescent microscopy may itself induce the dye coupling in previously uncoupled epithelial cells.

journal_name

Exp Cell Res

authors

Sharovskaja YuYu,Chailakhjan LM,Margolis LB

doi

10.1016/0014-4827(88)90204-2

subject

Has Abstract

pub_date

1988-04-01 00:00:00

pages

404-8

issue

2

eissn

0014-4827

issn

1090-2422

journal_volume

175

pub_type

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