Induction of tyrosinase in human melanoma cells by L-tyrosine phosphate and cytochalasin D.

abstract：:Mitotic BHK21 cells were examined by immunofluorescence optical sectioning after staining with anti-vimentin. Throughout all stages of mitosis, intact 10-nm filaments were observed to form a cage around the mitotic spindle. These observations were confirmed by serial section electron microscopy which demonstrated asse...

journal_title：Experimental cell research

authors： Blose SH,Bushnell A

更新日期：1982-11-01 00:00:00

abstract：BACKGROUND:Breast cancer is the major cause of cancer-related deaths in females world-wide. Doxorubicin-based therapy has limited efficacy in breast cancer due to drug resistance, which has been shown to be associated with the epithelial-to-mesenchymal transition (EMT). However, the molecular mechanisms linking the EMT...

journal_title：Experimental cell research

authors： Liu Y,Du F,Chen W,Yao M,Lv K,Fu P

更新日期：2013-12-10 00:00:00

abstract：:Recruitment of mesenchymal stem cells (MSC) to tissue damages is a promising approach for in situ tissue regeneration. The physiological mechanisms and regulatory processes of MSC trafficking to injured tissue remain poorly understood. However, the pivotal role of chemokines in MSC recruitment has already been shown. ...

journal_title：Experimental cell research

authors： Binger T,Stich S,Andreas K,Kaps C,Sezer O,Notter M,Sittinger M,Ringe J

更新日期：2009-05-01 00:00:00

abstract：:We previously identified a novel centrosomal protein CPAP, which carries a 112-residue motif that is essential for microtubule destabilization. In this report, we define both the microtubule (MT) binding and destabilizing domains in human CPAP and analyze the mutations that affect its MT-destabilizing activity. Analys...

journal_title：Experimental cell research

authors： Hsu WB,Hung LY,Tang CJ,Su CL,Chang Y,Tang TK

更新日期：2008-08-15 00:00:00

abstract：:ZO-2 is a MAGUK protein that in confluent epithelial sheets localizes at tight junctions (TJ) whereas in sparse cultures accumulates in clusters at the nucleus. Here, we have characterized several nuclear properties of ZO-2. We observe that ZO-2 is present in the nuclear matrix and co-immunoprecipitates with lamin B(1...

journal_title：Experimental cell research

authors： Jaramillo BE,Ponce A,Moreno J,Betanzos A,Huerta M,Lopez-Bayghen E,Gonzalez-Mariscal L

更新日期：2004-07-01 00:00:00

abstract：:Formation of the neural tube is the morphological hallmark for development of the embryonic central nervous system (CNS). Therefore, neural tube development is a crucial step in the neurulation process. Slit/Robo signaling was initially identified as a chemo-repellent that regulated axon growth cone elongation, but it...

journal_title：Experimental cell research

authors： Wang G,Li Y,Wang XY,Han Z,Chuai M,Wang LJ,Ho Lee KK,Geng JG,Yang X

更新日期：2013-05-01 00:00:00

abstract：:Synthetic tubugis are equally potent but more stable than their natural forms. Their anticancer potential was estimated on a solid melanoma in vitro and in vivo. Tubugi-1 induced the apoptosis in B16 cells accompanied with strong intracellular production of reactive species, subsequently imposing glutathione and thiol...

journal_title：Experimental cell research

authors： Drača D,Mijatović S,Krajnović T,Pristov JB,Đukić T,Kaluđerović GN,Wessjohann LA,Maksimović-Ivanić D

更新日期：2019-07-15 00:00:00

abstract：:Osteopontin (OPN) is a multifunctional protein implicated in cellular adhesion and migration. Phosphorylation has emerged as a post-translational modification important for certain biological activities of OPN. This study demonstrates that adhesion of isolated neonatal rat osteoclasts in vitro was augmented on bovine ...

journal_title：Experimental cell research

authors： Ek-Rylander B,Andersson G

更新日期：2010-02-01 00:00:00

abstract：:The binding of actinomycin D (actD) to fixed human metaphase chromosomes was studied by using autoradiography with [3H]actD and indirect immunofluorescence with a specific anti-actD antibody. At concentrations of 0.01 and 0.1 micrograms/ml there was a uniform distribution of drug along the chromosomes as observed by b...

journal_title：Experimental cell research

authors： Brothman AR,Lindell TJ

更新日期：1984-03-01 00:00:00

abstract：:KRIT1 is a disease gene responsible for Cerebral Cavernous Malformations (CCM). It encodes for a protein containing distinct protein-protein interaction domains, including three NPXY/F motifs and a FERM domain. Previously, we isolated KRIT1B, an isoform characterized by the alternative splicing of the 15th coding exon...

journal_title：Experimental cell research

authors： Francalanci F,Avolio M,De Luca E,Longo D,Menchise V,Guazzi P,Sgrò F,Marino M,Goitre L,Balzac F,Trabalzini L,Retta SF

更新日期：2009-01-15 00:00:00

abstract：:The influence of the cylindrical substratum surface on the actin microfilament bundle and the extracellular matrix (fibronectin and laminin) patterns in nontransformed epithelial cells of the IAR-2 rat line and in their N-ras-transformed descendants, IAR-ras-c4 cells, was studied. The cells were cultured on substrata ...

journal_title：Experimental cell research

authors： Levina EM,Domnina LV,Rovensky YA,Vasiliev JM

更新日期：1996-11-25 00:00:00

abstract：:Despite great progress for two decades in microRNAs (miRNAs), the direct regulation of host gene by intragenic (mostly intronic) miRNA is conceptually plausible but evidence-limited. Here, we report that intronic miR-932 could target its host gene via binding with coding sequence (CDS) region rather than regular 3'UTR...

journal_title：Experimental cell research

authors： Qian J,Tu R,Yuan L,Xie W

更新日期：2016-06-10 00:00:00

abstract：:RNA polymerase II transcripts are confined to nuclear compartments. A detailed analysis of the nuclear topology of RNA from individual genes was performed for transcripts from the marker gene coding for chloramphenicol acetyltransferase, expressed at a high level from the HTLV-1 LTR promoter. The construct was transfe...

journal_title：Experimental cell research

authors： Bridger JM,Kalla C,Wodrich H,Weitz S,King JA,Khazaie K,Kräusslich HG,Lichter P

更新日期：2005-01-15 00:00:00

abstract：:Mammalian stanniocalcin-2 (STC2) is a secreted glycoprotein hormone with a putative role in unfolded protein response and apoptosis. Here we reported that STC2 expression was sporadically abrogated in human cancer cells by transcriptional silencing associated with CpG island promoter hypermethylation. Direct sequencin...

journal_title：Experimental cell research

authors： Law AY,Lai KP,Ip CK,Wong AS,Wagner GF,Wong CK

更新日期：2008-05-01 00:00:00

abstract：:The effect of SK&F 96365 (1-(beta-[3-(4-methoxyphenyl)propoxyl]-4- methoxyphenethyl)-1H-imidazole hydrochloride), a recently synthesized inhibitor of receptor-mediated calcium entry, was investigated on human hematopoietic cell lines. We found that treatment of the T-cell leukemia line Jurkat with SK&F 96365 inhibited...

journal_title：Experimental cell research

authors： Nordström T,Nevanlinna HA,Andersson LC

更新日期：1992-10-01 00:00:00

abstract：:We report that a specific inhibitor of types 1 and 2A phosphatases, okadaic acid (OA), induces germinal vesicle break down (GVBD) and chromosome condensation when microinjected into denuded mouse oocytes maintained in prophase block by analogs of cAMP, inhibitors of phosphodiesterase, or a tumor-promoting phorbol este...

journal_title：Experimental cell research

authors： Gavin AC,Tsukitani Y,Schorderet-Slatkine S

更新日期：1991-01-01 00:00:00

abstract：:Rat peritoneal mast cells (RPMC) and rat basophilic leukemia (RBL) cells are representative of connective tissue-type (CTMC) and mucosal-type (MMC) mast cells, respectively. Using polyethylene glycol, we have fused RPMC with 6-thioguanine resistant, HAT (hypoxanthine, aminopterin, thymidine) sensitive RBL-CA10.7 or RB...

journal_title：Experimental cell research

authors： Zheng YL,Chan BM,Rector ES,Berczi I,Froese A

更新日期：1991-06-01 00:00:00

abstract：:A panel of monoclonal antibodies, specific for human platelet (NM-A9, NM-F6, and NM-G2) and for bovine smooth muscle (SM-E7) myosin heavy chains (MHC), were used to study the composition and the distribution of myosin isoforms in bovine endothelial cells (EC), in vivo and in vitro. Using indirect and double immunofluo...

journal_title：Experimental cell research

authors： Borrione AC,Zanellato AM,Giuriato L,Scannapieco G,Pauletto P,Sartore S

更新日期：1990-09-01 00:00:00

abstract：:Embryonic stem (ES) cell lines have been derived from blastocysts of the inbred mouse strain C57BL/6. The highest frequencies of ES cell colonies were observed when blastocysts were explanted directly onto growth-arrested feeder layers of 5637 human bladder carcinoma cells in the presence of conditioned medium. One of...

journal_title：Experimental cell research

authors： Ledermann B,Bürki K

更新日期：1991-12-01 00:00:00

abstract：:The effect of RU 486, a synthetic steroid that is a powerful antagonist of glucocorticoid hormones, was tested on the transcription of several glucocorticoid-regulated genes in different cell types: inflammatory murine macrophages and two human mammary gland-derived cell lines, MDA-MB-231 and HBL-100. The transcriptio...

journal_title：Experimental cell research

authors： Busso N,Collart M,Vassalli JD,Belin D

更新日期：1987-12-01 00:00:00

abstract：:hnRNP I, also referred to as polypyrimidine tract binding protein, is one of the proteins associated with nascent pre-mRNA in the heterogeneous nuclear ribonucleoprotein (hnRNP) complexes. As for all karyophilic proteins, the nuclear import of hnRNP proteins requires specific sequence determinants that in many instanc...

journal_title：Experimental cell research

authors： Romanelli MG,Weighardt F,Biamonti G,Riva S,Morandi C

更新日期：1997-08-25 00:00:00

abstract：:Vigilin, a protein with a continuous series of 14 KH motifs, forms part of a multiprotein complex containing tRNA. Several lines of evidence have suggested that vigilin expression is enhanced in those cells which were actively engaged in protein synthesis. Accordingly, we show here by immunoelectronmicroscopy a close ...

journal_title：Experimental cell research

authors： Kruse C,Emmrich J,Rumpel E,Klinger MH,Grünweller A,Rohwedel J,Krammer HJ,Kühnel W,Müller PK

更新日期：1998-02-25 00:00:00

abstract：:A monoclonal antibody specific for vimentin is described which, by immunofluorescence and immunoelectron microscopy, decorates fibrillar and/or granular structures in mitotic and early postmitotic cells but does not react with vimentin filaments of interphase stages of various cultured cells (rat vascular smooth muscl...

journal_title：Experimental cell research

authors： Franke WW,Grund C,Kuhn C,Lehto VP,Virtanen I

更新日期：1984-10-01 00:00:00

abstract：:Swiss 3T3 cells can be made quiescent at low density by plating in medium MCDB 402 supplemented with dexamethasone (DEX), insulin (INS) and bovine plasma fibronectin (BPFn) for 3 days. One hour after stimulation of these cells by fibroblast growth factor (FGF), an increase in the rate of protein synthesis can be measu...

journal_title：Experimental cell research

authors： Shipley GD,Ham RG

更新日期：1983-07-01 00:00:00

abstract：:Human and rodent cells undergoing apoptosis were observed to express high levels of a novel 45,000 M(r) protein. The protein, which we have termed apoptosis specific protein (ASP), was found in Burkitt lymphoma (BL) cells and in adenovirus-transformed human and rat embryo cells induced into apoptosis by a variety of s...

journal_title：Experimental cell research

authors： Grand RJ,Milner AE,Mustoe T,Johnson GD,Owen D,Grant ML,Gregory CD

更新日期：1995-06-01 00:00:00

abstract：:There is increasing evidence that the hypersensitive response during plant-pathogen interactions is a form of programmed cell death. In an attempt to understand the biochemical nature of this form of programmed cell death in the cowpea-cowpea rust fungus system, proteolytic activity in extracts of fungus-infected and ...

journal_title：Experimental cell research

authors： D'Silva I,Poirier GG,Heath MC

更新日期：1998-12-15 00:00:00

abstract：:KILLER/DR5 is a death-domain-containing proapoptotic receptor that binds to the cytotoxic ligand TRAIL. It was originally reported that induction of KILLER/DR5 mRNA following DNA damage was p53-dependent, but some drugs that induce apoptosis can upregulate KILLER/DR5 mRNA expression in cell lines with mutated p53. We ...

journal_title：Experimental cell research

authors： Meng RD,El-Deiry WS

更新日期：2001-01-15 00:00:00

abstract：:The phosphorylation of glucose-regulated protein, GRP78, is thought to be involved in the regulation of the binding function of GRP78 to immunoglobulin heavy chains. The phosphorylation of GRP78 proceeded faster in transformed cells than in normal cells, whereas the levels of GRP78 synthesis and accumulation were simi...

journal_title：Experimental cell research

authors： Satoh M,Nakai A,Sokawa Y,Hirayoshi K,Nagata K

更新日期：1993-03-01 00:00:00

abstract：:Previous studies have shown that calpains are autolytically cleaved during the disease process of mdx dystrophy, a mouse model for Duchenne muscular dystrophy, indicating that calpains may be activated and play a role in proteolysis that occurs in muscular dystrophy (J. Biol. Chem. 270(18), 10909-10914, 1995). In the ...

journal_title：Experimental cell research

authors： Spencer MJ,Tidball JG

更新日期：1996-08-01 00:00:00

abstract：:In human and non-human primates, migratory trophoblasts penetrate the uterine epithelium, invade the endometrium, enter the uterine vasculature, and migrate within the arteries. The mechanisms that regulate this directional migration are unknown. We have used early gestation macaque trophoblasts to test the hypothesis...

journal_title：Experimental cell research

authors： Thirkill TL,Lowe K,Vedagiri H,Blankenship TN,Barakat AI,Douglas GC

更新日期：2005-05-01 00:00:00