Abstract：
:The phosphorylation of glucose-regulated protein, GRP78, is thought to be involved in the regulation of the binding function of GRP78 to immunoglobulin heavy chains. The phosphorylation of GRP78 proceeded faster in transformed cells than in normal cells, whereas the levels of GRP78 synthesis and accumulation were similar in both cells. Treatment of the cells with tunicamycin caused a rapid decrease in GRP78 phosphorylation within 2 to 4 h in both cell types prior to GRP78 induction. Following a longer period of tunicamycin treatment, GRP78 phosphorylation recovered gradually in parallel with the accumulation of newly synthesized GRP78. The half-life of GRP78 was over 24 h and similar in both normal and transformed cells either with or without tunicamycin treatment. In contrast, the half-life of phosphate groups incorporated into GRP78 was about 120 min in both types of cells in the absence of tunicamycin treatment. When the cells were treated with tunicamycin, the half-life of the phosphate groups was shortened (-30 min) only in transformed cells, while it remained at untreated control levels in normal cells. These results suggest that GRP78 phosphorylation is important in functional regulation, and that the cells may carry out particular requirements such as increasing or decreasing secretory proteins by modulating GRP78 phosphorylation rather than GRP78 synthesis.
journal_name
Exp Cell Resjournal_title
Experimental cell researchauthors
Satoh M,Nakai A,Sokawa Y,Hirayoshi K,Nagata Kdoi
10.1006/excr.1993.1060subject
Has Abstractpub_date
1993-03-01 00:00:00pages
76-83issue
1eissn
0014-4827issn
1090-2422pii
S0014-4827(83)71060-8journal_volume
205pub_type
杂志文章abstract：:The effect of the cyclin-dependent (CDK) inhibitors olomoucine and roscovitine on cell kinetics was studied. To this end, nonsmall cell lung cancer (NSCLC) cell line MR65 and neuroblastoma cell line CHP-212 were pulse labeled with bromodeoxyuridine (BrdUrd) and chased in culture medium, to which various concentrations...
journal_title：Experimental cell research
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journal_title：Experimental cell research
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更新日期：2018-09-15 00:00:00
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journal_title：Experimental cell research
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1006/excr.1993.1098
更新日期：1993-04-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0014-4827(92)90041-6
更新日期：1992-11-01 00:00:00
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更新日期：2018-02-01 00:00:00
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journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(91)90351-t
更新日期：1991-06-01 00:00:00
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pub_type： 杂志文章
doi：10.1016/0014-4827(91)90423-r
更新日期：1991-12-01 00:00:00
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doi：10.1016/j.yexcr.2004.04.011
更新日期：2004-08-01 00:00:00
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doi：10.1016/j.yexcr.2003.11.030
更新日期：2004-04-01 00:00:00
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更新日期：2017-01-01 00:00:00
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doi：10.1016/j.yexcr.2016.10.024
更新日期：2016-12-10 00:00:00
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pub_type： 杂志文章
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更新日期：2004-09-10 00:00:00
abstract：:The expression patterns of the testis-specific and somatic forms of the pyruvate dehydrogenase (PDH) E1 alpha subunit genes were examined in adult mouse testis by in situ hybridization with specific cDNA probes and by immunostaining. A considerable increase in the mRNA level of the testis-specific PDH E1 alpha gene wa...
journal_title：Experimental cell research
pub_type： 杂志文章
doi：10.1016/0014-4827(92)90459-l
更新日期：1992-03-01 00:00:00
abstract：:Clear cell renal cell carcinoma (ccRCC) is one of the most common malignant tumors in the urinary system, and its incidence continues to increase. Regulator of calcineurin 1 (RCAN1), one of the genes on chromosome 21, is a crucial mediator of tumor inhibition. RCAN1.4 is best characterized as an endogenous inhibitor o...
journal_title：Experimental cell research
pub_type： 杂志文章
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更新日期：2018-11-15 00:00:00
