Overexpression of ABCG2 confers resistance to pevonedistat, an NAE inhibitor.

Abstract:

:Pevonedistat is a potent, selective, first-in-class NEDD8 activating enzyme inhibitor. It is now under multiple clinical trials that investigate its anticancer effect against solid tumors and leukemia. ATP-binding cassette (ABC) transporters are membrane proteins that are involved in mediating multidrug resistance (MDR). In this article, we reveal that pevonedistat is a substrate of ABCG2 which decreases the therapeutic effect of pevonedistat. The cytotoxicity of pevonedistat was significantly weakened in ABCG2-overexpressing cells, and the drug resistance can be reversed by ABCG2 inhibitors. The ATPase assay suggested that pevonedistat can stimulate ABCG2 ATPase activity in a concentration-dependent manner. Pevonedistat showed little effect on the expression level or subcellular localization of ABCG2 after 72 h treatment. Furthermore, a pevonedistat resistance cell line S1-PR was established and overexpressed ABCG2. Generally, our study provides evidence that ABCG2 can be a prominent factor leading to pevonedistat-resistance. Furthermore, ABCG2 may also be utilized as a biomarker to monitor the development of pevonedistat resistance during cancer treatment.

journal_name

Exp Cell Res

authors

Wei LY,Wu ZX,Yang Y,Zhao M,Ma XY,Li JS,Yang DH,Chen ZS,Fan YF

doi

10.1016/j.yexcr.2020.111858

subject

Has Abstract

pub_date

2020-03-15 00:00:00

pages

111858

issue

2

eissn

0014-4827

issn

1090-2422

pii

S0014-4827(20)30052-5

journal_volume

388

pub_type

杂志文章
  • Differential regulation of extracellular signal-regulated kinase 1 and 2 activity during 12-O-tetradecanoylphorbol 13-acetate-induced differentiation of HL-60 cells.

    abstract::In this study we have analyzed short- and long-term changes in extracellular signal-regulated kinase (ERK) 1 and 2 activity during 12-O-tetradecanoylphorbol 13-acetate (TPA)-induced differentiation of human promyelocytic leukemia cells. Immunoprecipitation of HL-60 cellular extracts with an ERK antibody followed by in...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1997.3631

    authors: Meighan-Mantha RL,Wellstein A,Riegel AT

    更新日期:1997-08-01 00:00:00

  • C-banding of Peromyscus constitutive heterochromatin persists following histone hyperacetylation.

    abstract::Approx. 35% of the DNA of cultured cells from the cactus mouse, Peromuscus eremicus, is contained in highly condensed constitutive heterochromatin which can be visualized in metaphase chromosomes stained by the C-band technique. Previous studies have shown this constitutive heterochromatin to contain a large proportio...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(83)90209-4

    authors: Halleck MS,Schlegel RA

    更新日期:1983-09-01 00:00:00

  • Consequences of Shb and c-Abl interactions for cell death in response to various stress stimuli.

    abstract::The adaptor protein Shb has previously been shown to regulate apoptosis in response to cytokines and inhibitors of angiogenesis although the mechanisms governing these effects have remained obscure. We currently demonstrate interactions between Shb and c-Abl and that Shb regulates c-Abl kinase activity. The data sugge...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2006.10.011

    authors: Hägerkvist R,Mokhtari D,Lindholm C,Farnebo F,Mostoslavsky G,Mulligan RC,Welsh N,Welsh M

    更新日期:2007-01-15 00:00:00

  • A vacuolar-type proton ATPase mediates acidification of plasmalemmal vesicles during potocytosis.

    abstract::Previously we showed that the potocytosis of 5-methyltetrahydrofolate is dependent on the acidification of plasmalemmal vesicles created each time a caveolae, closes off from the cell surface. We now report that bafilomycin A1, which is a specific inhibitor of the V-type proton ATPase, inhibits 5-methyltetrahydrofolat...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1996.0133

    authors: Mineo C,Anderson RG

    更新日期:1996-05-01 00:00:00

  • Enhanced expression of heat shock proteins in gradually dying cells and their release from necrotically dead cells.

    abstract::Heat shock proteins (HSPs) have molecular chaperone functions in protein biogenesis as well as cytoprotective functions against deleterious environmental stresses, and they work mainly inside of the cells. HSPs are usually induced in living cells that have been exposed to mild stresses or have recovered from severe st...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2005.07.014

    authors: Saito K,Dai Y,Ohtsuka K

    更新日期:2005-10-15 00:00:00

  • The role of HGF on invasive properties and repopulation potential of human fetal hepatic progenitor cells.

    abstract:UNLABELLED:The success of hepatocyte transplantation has been limited by the low efficiency of transplanted cell integration into liver parenchyma. Human fetal hepatic progenitor cells (hepatoblasts) engraft more effectively than adult hepatocytes in mouse livers. However, the signals required for their integration are...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2009.07.007

    authors: Delgado JP,Vanneaux V,Branger J,Touboul T,Sentilhes L,Mainot S,Lainas P,Leclerc P,Uzan G,Mahieu-Caputo D,Weber A

    更新日期:2009-11-15 00:00:00

  • Morphology of endoplasmic reticulum and Golgi elements following microinjection of rat liver microsomes into Xenopus laevis oocyte cytoplasm.

    abstract::Fragments of rough endoplasmic reticulum and Golgi complex purified from rat liver homogenates were injected into Xenopus oocytes and the sites of microinjection analysed by electron microscopy at different times post-injection. The in vivo incubated fragments were located by their proximity to a microinjection vacuol...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(86)90495-7

    authors: Paiement J

    更新日期:1986-10-01 00:00:00

  • MicroRNA library screening identifies growth-suppressive microRNAs that regulate genes involved in cell cycle progression and apoptosis.

    abstract::Micro(mi)RNAs play important and varied roles in tumorigenesis; however, the full repertoire of miRNAs that affect cancer cell growth is not known. In this study, an miRNA library was screened to identify those that affect the growth of A549 tumor cells. Among 300 miRNAs, miR-28-5p, -323-5p, -510-5p, -552-3p, and -608...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2015.10.012

    authors: Choi YC,Yoon S,Byun Y,Lee G,Kee H,Jeong Y,Yoon J,Baek K

    更新日期:2015-12-10 00:00:00

  • Downregulation of endogenous STAT3 augments tumoricidal activity of interleukin 15 activated dendritic cell against lymphoma and leukemia via TRAIL.

    abstract::Effector functions in tumor resistance by dendritic cells (DCs) are less well characterized. In this study, we describe that the murine DCs upon stimulation with recombinant IL-15 in vitro or in vivo, expresses TNF superfamily member TRAIL which mediates cytotoxicity and growth inhibition against a murine lymphoma cal...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2014.08.012

    authors: Hira SK,Mondal I,Bhattacharya D,Manna PP

    更新日期:2014-10-01 00:00:00

  • A sequential staining technique for karyotypic analysis of interspecific somatic cell hybrids.

    abstract::We describe a sequential staining technique for the karyotypic analysis of interspecific mouse x human somatic cell hybrids. Fluorescence in situ hybridization of samples, previously stained using standard trypsin/Giemsa protocols, was instrumental in the identification of human chromosomes present in hybrid lines. Th...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1993.1098

    authors: Keherly MJ,Papaconstantinou J,McCombs JL

    更新日期:1993-04-01 00:00:00

  • Characterization of density-dependent regulation of the tyrosinase gene promoter: role of protein kinase C.

    abstract::The rate-limiting step in melanogenesis is catalyzed by tyrosinase, a multifunctional enzyme encoded by the albino locus. We have previously reported that depletion of protein kinase C by long-term treatment of B16 mouse melanoma cells with phorbol dibutyrate (PDBu) prevented cell density-dependent melanogenesis. This...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1997.3773

    authors: Mahalingam H,Watanabe A,Tachibana M,Niles RM

    更新日期:1997-11-25 00:00:00

  • Collagen XIV and a related recombinant fragment protect human vascular smooth muscle cells from calcium-/phosphate-induced osteochondrocytic transdifferentiation.

    abstract::Transdifferentiation of vascular smooth muscle cells (VSMC) promotes the development of vascular calcifications such as arteriosclerosis. The aim was to investigate effects of specific extracellular matrix (ECM) components on transdifferentiation of VSMC to identify novel ECM-based therapeutic tools. Human collagens I...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2017.06.018

    authors: Freise C,Bobb V,Querfeld U

    更新日期:2017-09-15 00:00:00

  • Subcellular distribution, secretion, and posttranslational modifications of clusterin in thyrocytes.

    abstract::A prominent secretory glycoprotein was detected in the culture medium of porcine thyrocytes which was identified as clusterin by microsequencing. Treatment of thyrocytes with thyroid stimulating hormone revealed a tight regulation of both synthesis and secretion of clusterin, with a distinct fraction of clusterin bein...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1999.4555

    authors: Lemansky P,Brix K,Herzog V

    更新日期:1999-08-25 00:00:00

  • Cell condensation in chondrogenic differentiation.

    abstract::Reduction of intercellular spaces in the areas of prospective cartilage and bone formation (precartilage condensation) precedes chondrogenesis and may represent an important step in the process of cartilage differentiation during limb skeletogenesis. We have attempted to clarify the role of the microenvironment establ...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/s0014-4827(05)80067-9

    authors: Tacchetti C,Tavella S,Dozin B,Quarto R,Robino G,Cancedda R

    更新日期:1992-05-01 00:00:00

  • Distinction of G0 cells from senescent cells in cultures of non-cycling human fetal lung fibroblasts by anti-MAP-1 monoclonal antibody staining.

    abstract::On staining with a monoclonal antibody raised against microtubule-associated protein-1 (MAP-1), dot-like structures were seen in the nuclei of interphase cells, but not in those of non-cycling G0-arrested cells. Dots were also not seen in the nuclei of non-cycling senescent human cells (IMR-90). A SV40-DNA-transformed...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(86)90062-5

    authors: Ohno T,Kako R,Sato C,Ohkawa A

    更新日期:1986-04-01 00:00:00

  • Osmotic swelling of endocytic compartments induced by internalized sucrose is restricted to mature lysosomes in cultured mammalian cells.

    abstract::To determine which endocytic compartments are sensitive to sucrose-induced osmotic swelling, CHO and Vero cells were cultured for 1-3 days in media containing 0.03 to 0.05 M sucrose. (Sucrose is internalized but not digested by these cells.) To immunolocalize late endocytic compartments, cells were fixed with formalde...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(91)90156-o

    authors: DeCourcy K,Storrie B

    更新日期:1991-01-01 00:00:00

  • Inflammation-driven colon neoplasmatogenesis in uPA-deficient mice is associated with an increased expression of Runx transcriptional regulators.

    abstract::Deregulation of the bone morphogenetic protein (BMP) pathway has been documented in colorectal cancer (CRC). Previously, we investigated possible associations between urokinase-type plasminogen activator (uPA) deficiency and expression of extracellular constituents of BMP signaling in a newly developed mouse model of ...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2017.10.025

    authors: Afaloniati H,Karagiannis GS,Hardas A,Poutahidis T,Angelopoulou K

    更新日期:2017-12-15 00:00:00

  • Induction of M-phase entry of prophase-blocked mouse oocytes through microinjection of okadaic acid, a specific phosphatase inhibitor.

    abstract::We report that a specific inhibitor of types 1 and 2A phosphatases, okadaic acid (OA), induces germinal vesicle break down (GVBD) and chromosome condensation when microinjected into denuded mouse oocytes maintained in prophase block by analogs of cAMP, inhibitors of phosphodiesterase, or a tumor-promoting phorbol este...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(91)90159-r

    authors: Gavin AC,Tsukitani Y,Schorderet-Slatkine S

    更新日期:1991-01-01 00:00:00

  • Analysis of 3-phosphoinositide-dependent kinase-1 signaling and function in ES cells.

    abstract::3-phosphoinositide-dependent kinase-1 (PDK1) phosphorylates and activates several kinases in the cAMP-dependent, cGMP-dependent and protein kinase C (AGC) family. Many putative PDK1 substrates have been identified, but have not been analyzed following transient and specific inhibition of PDK1 activity. Here, we demons...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2008.04.006

    authors: Tamgüney T,Zhang C,Fiedler D,Shokat K,Stokoe D

    更新日期:2008-07-01 00:00:00

  • Abnormal sperm parameters in humans are indicative of an abortive apoptotic mechanism linked to the Fas-mediated pathway.

    abstract::The life cycle of many cell types can hinge on the presence of death factors that can control programmed cell death. The Fas-mediated apoptotic pathway has been implicated in controlling apoptosis during spermatogenesis in a number of mammalian species. In the human, the presence of nuclear DNA damage in ejaculated sp...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1999.4586

    authors: Sakkas D,Mariethoz E,St John JC

    更新日期:1999-09-15 00:00:00

  • Resveratrol decreases TNFα-induced ICAM-1 expression and release by Sirt-1-independent mechanism in intestinal myofibroblasts.

    abstract::Up-regulation of intercellular adhesion molecule-1 (ICAM-1) and its soluble form are involved in the chronic inflammation. For the first time, we demonstrated that resveratrol (RE), a natural polyphenol with antioxidant and anti-inflammatory properties, reduces the increase of expression and release of ICAM-1, due to ...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2019.06.024

    authors: Domazetovic V,Bonanomi AG,Stio M,Vincenzini MT,Iantomasi T

    更新日期:2019-09-15 00:00:00

  • EAT/mcl-1 expression in the human embryonal carcinoma cells undergoing differentiation or apoptosis.

    abstract::Differentiation and apoptosis are precisely regulated events in early embryogenesis. Retinoic acid-induced differentiation in the embryonal carcinoma (EC) cell line NCR-G3 triggers concurrent induction of apoptosis. Using this system, which serves as a model of early embryogenesis, the expression of various bcl-2-rela...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.2001.5203

    authors: Sano M,Umezawa A,Abe H,Akatsuka A,Nonaka S,Shimizu H,Fukuma M,Hata J

    更新日期:2001-05-15 00:00:00

  • The regulation of transcriptional repression in hypoxia.

    abstract::A sufficient supply molecular oxygen is essential for the maintenance of physiologic metabolism and bioenergetic homeostasis for most metazoans. For this reason, mechanisms have evolved for eukaryotic cells to adapt to conditions where oxygen demand exceeds supply (hypoxia). These mechanisms rely on the modification o...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审

    doi:10.1016/j.yexcr.2017.02.024

    authors: Cavadas MAS,Cheong A,Taylor CT

    更新日期:2017-07-15 00:00:00

  • Shape of my heart: Cell-cell adhesion and cytoskeletal dynamics during Drosophila cardiac morphogenesis.

    abstract::The fruit fly Drosophila melanogaster has recently emerged as an excellent system to investigate the genetics of cardiovascular development and disease. Drosophila provides an inexpensive and genetically-tractable in vivo system with a large number of conserved features. In addition, the Drosophila embryo is transpare...

    journal_title:Experimental cell research

    pub_type: 杂志文章,评审

    doi:10.1016/j.yexcr.2017.03.057

    authors: McFaul CMJ,Fernandez-Gonzalez R

    更新日期:2017-09-01 00:00:00

  • Tks5 recruits AFAP-110, p190RhoGAP, and cortactin for podosome formation.

    abstract::Podosome formation in vascular smooth muscle cells is characterized by the recruitment of AFAP-110, p190RhoGAP, and cortactin, which have specific roles in Src activation, local down-regulation of RhoA activity, and actin polymerization, respectively. However, the molecular mechanism that underlies their specific recr...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/j.yexcr.2009.06.012

    authors: Crimaldi L,Courtneidge SA,Gimona M

    更新日期:2009-09-10 00:00:00

  • Caloric restriction: conservation of cellular replicative capacity in vitro accompanies life-span extension in mice.

    abstract::We have tested whether life-long caloric restriction (CR) slows or delays the age-related loss of cellular replicative potential that occurs during normal aging in ad libitum (AL) fed mice. Both mean and maximum life spans of the restricted animals (60% of AL intake) were significantly extended 30-40% by CR treatment....

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1995.1091

    authors: Pendergrass WR,Li Y,Jiang D,Fei RG,Wolf NS

    更新日期:1995-04-01 00:00:00

  • Cytosolic free calcium spiking affected by intracellular pH change.

    abstract::The characteristics underlying cytosolic free calcium oscillation were evaluated by superfused dual wave-length microspectrofluorometry of fura-2-loaded single acinar cells from rat pancreas. Application of a physiological concentration of cholecystokinin octapeptide (CCK) (20 pM) induced a small basal increase in cyt...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/0014-4827(90)90173-8

    authors: Tsunoda Y

    更新日期:1990-06-01 00:00:00

  • Commitment of 3T3-F442A cells to adipocyte differentiation takes place during the first 24-36 h after adipogenic stimulation: TNF-alpha inhibits commitment.

    abstract::We studied the commitment of 3T3-F442A cells during stimulation with adipogenic serum or growth hormone. Confluent 3T3-F442A preadipocytes were incubated with adipogenic medium for increasing times; the number of adipose clusters, GPDH activity, and lipid accumulation were evaluated. Results show that cell commitment ...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1016/s0014-4827(02)00036-8

    authors: Castro-Muñozledo F,Beltrán-Langarica A,Kuri-Harcuch W

    更新日期:2003-04-01 00:00:00

  • 50-kb chromatin fragmentation in the absence of apoptosis.

    abstract::Treatment with ionic detergents of nuclei isolated from various continuously growing cell lines generally yields chromatin samples of high viscosity. Extensive treatment with nuclease-free proteinase K or pronase solubilized the viscous lysates with > 90% of the DNA migrating at approximately 50 kb. Freshly prepared h...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1995.1381

    authors: Szabó G Jr

    更新日期:1995-12-01 00:00:00

  • Establishment of the permanent microvascular endothelial cell line PBMEC/C1-2 from porcine brains.

    abstract::Porcine brain microvascular endothelial cells (PBMEC) were isolated from fresh brains by several enzymatic digestion steps followed by a gradient centrifugation. Cells of the primary culture were transfected with pRNS-1, encoding for the small and large T-antigens of SV 40, by means of lipofection. After selection wit...

    journal_title:Experimental cell research

    pub_type: 杂志文章

    doi:10.1006/excr.1996.0298

    authors: Teifel M,Friedl P

    更新日期:1996-10-10 00:00:00